中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (10): 35-43.DOI: 10.13304/j.nykjdb.2022.0198

• 生物技术 生命科学 • 上一篇    下一篇

草甘膦铵盐诱导谷子雄性不育的转录组分析

郭瑞锋1(), 任月梅1(), 杨忠1, 刘贵山2, 任广兵1, 张绶1, 朱文娟1   

  1. 1.山西农业大学高寒区作物研究所,山西 大同 037008
    2.五寨县农业农村局,山西 忻州 036200
  • 收稿日期:2022-03-15 接受日期:2022-05-28 出版日期:2022-10-15 发布日期:2022-10-25
  • 通讯作者: 任月梅
  • 作者简介:郭瑞锋 E-mail:guoruifeng229@126.com
  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-06-14.5-B7);山西农业大学生物育种工程项目(YZGC079);山西省谷子产业技术体系项目(GZTX202210);杂粮种质创新与分子育种国家实验室项目(202204010910001);国家重点研发计划项目(2021YFD1901103-5)

Transcriptomic Analysis of Mechanism of Foxtail Millet Male Infertility Induced by Glyphosate Ammonium Salt

Ruifeng GUO1(), Yuemei REN1(), Zhong YANG1, Guishan LIU2, Guangbing REN1, Shou ZHANG1, Wenjuan ZHU1   

  1. 1.High Latitude Crops Institute, Shanxi Agricultural University, Shanxi Datong 037008, China
    2.Bureau of Agriculture and Rural Affairs of Wuzhai County, Shanxi Xinzhou 036200, China
  • Received:2022-03-15 Accepted:2022-05-28 Online:2022-10-15 Published:2022-10-25
  • Contact: Yuemei REN

摘要:

为促进谷子杂种优势的利用,提高杂交育种效率,采用 Illumina HiSeq 高通量测序技术对草甘膦铵盐处理后的谷穗进行转录组测序,分析筛选响应草甘膦铵盐的差异表达基因,同时测定谷穗抗性淀粉、可溶性糖等生理指标。结果表明,草甘膦铵盐处理后产生的雄性不育谷穗与未处理谷穗相比,共检测到797个差异表达基因,其中645个差异表达基因获得GO功能分类,主要集中在糖代谢与生物合成、激素代谢和生物合成以及细胞壁等方面,反映了谷穗对草甘膦铵盐处理响应基因的生物学功能。通过KEGG富集分析,共建立了138条通路,发现差异表达基因中涉及植物激素信号转导途径的最多,为19个;其次是淀粉和蔗糖代谢途径,为16个。经诱导产生雄性不育的谷穗样品中抗性淀粉、可溶性糖和赤霉素含量降低,生长素含量增加,与GO和KEGG富集分析得到的差异表达基因的表达模式相吻合。以上结果为筛选适宜的化学杀雄试剂提供了理论依据。

关键词: 谷子, 草甘膦铵盐, 诱导雄性不育, 差异表达基因, 生理指标

Abstract:

To promote the heterosis utilization of foxtail millet and improve the efficiency of cross breeding, transcriptome sequencing of the grain spike treated by ammonium glyphosate was performed using the Illumina HiSeq high-throughput sequencing technology, the differentially expressed genes were analytically screened among different treatments. The physiological indexes such as resistant starch and soluble sugar were determined. The results showed that the grain ears treated with glyphosate had 797 differentially expressed genes compared with untreated ears, among which 645 differentially expressed genes obtained GO functional classification, and they mainly focused on glucose metabolism and biosynthesis, hormone metabolism and biosynthesis and cell wall, reflecting the biological function of response genes to glyphosate treatment. A total of 138 pathways were established by KEGG enrichment analysis, including 19 differentially expressed genes involved in the plant hormone signaling pathway and 16 differentially expressed genes involved in the starch and sucrose metabolism pathways. In the grain spike treated by ammonium glyphosate, the contents of resistant starch, soluble sugar and gibberellin decreased, while the IAA content increased, which were consistent with the expression pattern of differeatly expressed genes by GO and KEGG enrichment analysis. These above results provided a theoretical basis for screening suitable chemical androcide reagents.

Key words: foxtail millet, glyphosate ammonium salt, induced male sterility, differentially expressed genes, physiological indicators

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