关键词: 产气荚膜梭菌, 长双歧杆菌, 表乳糖, 共培养, 抑菌

Abstract: Clostridium perfringens is a common pathogen in human, livestock and poultry, which can cause acute food poisoning, chronic intestinal infection in human or necrotizing enteritis in animals. It seriously endangers human health and the development of livestock and poultry breeding. In this paper, the effects of the specific carbon sources such as epilactose and its isomers lactose and lactulose were studied by the single-strain culture of Bifidobacterium longum and the co-culture of B. longum and C. perfringens. The results showed that B. longum could grow well in mTPY medium with epilactose as a carbon source and showed a similar growth trend as lactose. In addition, B. longum could significantly inhibit the growth of C. perfringens in a co-culture system, and the viability of B. longum in the epilactose medium was significantly better than that in lactose and lactulose ones. Moreover, an oxford cup test was also applied to measure the antibacterial effect of B. longum on C. perfringens, showing that the culture supernatant of B. longum had a good antibacterial activity against C. perfringens. These results provided theoretical reference for the inhibition of C. perfringens by B. longum, and also provide important guidance for further study of epilactose and its regulation mechanism.

Key words: Clostridium perfringens, Bifidobacterium longum, epilactose, co-culture, bacteriostatics