关键词: 东方百合&lsquo, 索邦&rsquo, 愈伤诱导, 增殖, 生根诱导

Abstract: As the main market fresh cut lily variety, Lilium orential ‘Sorbonne’ has great market potential. In order to meet the market demand, flower organs were used as initial materials to study the callus induction, cluster bud proliferation and rooting culture of L. orential ‘Sorbonne’ in vitro culture. The results showed that ovary was the appropriate initial explant for callus induction, and the lower part of leaf or bulb was the appropriate explant for further propagation. The suitable medium for callus induction was MS+ 2.0 mg·L^-1 2,4-D+0.2 mg·L^-1 6-BA+30 g·L^-1 sucrose+6.5 g·L^-1 agar, pH 5.8. Cluster bud proliferation medium was MS+ l.0 mg·L^-1 6-BA + 0.l mg·L^-1 NAA +30 g·L^-1 sucrose+6.5 g·L^-1 agar, pH 5.8. Rooting culture medium was MS+0.5 mg·L^-1 NAA+30 g·L^-1 sucrose+6.5 g·L^-1 agar, pH 5.8. The results provided a basis for further improving the in vitro culture system of L. orential ‘Sorbonne’ and for large-scale production of high-quality seed bulbs.

Key words: Lilium orential ‘Sorbonne’, callus induction, proliferation, roots induction