燕麦根际土壤细菌多样性对盐胁迫的响应

doi:10.13304/j.nykjdb.2021.0906

中国农业科技导报 ›› 2023, Vol. 25 ›› Issue (1): 153-165.DOI: 10.13304/j.nykjdb.2021.0906

• 生物制造资源生态 • 上一篇

燕麦根际土壤细菌多样性对盐胁迫的响应

李峰¹(), 殷丛培²(), 殷冉³, 王凡², 韩永亮⁴, 杨志敏¹(), 刘建成¹()

^1.张家口市农业科学院，河北张家口 075000
^2.河北农业大学农学院，河北保定 071000
^3.河北农业大学植物保护学院，河北保定 071000
^4.邯郸市农业科学院，河北邯郸 056000

收稿日期:2021-10-25 接受日期:2022-03-08 出版日期:2023-01-15 发布日期:2023-04-17
通讯作者: 杨志敏,刘建成
作者简介:李峰 E-mail：20234350@qq.com
殷丛培 E-mail：ycongpei2015@163.com第一联系人：李峰和殷丛培为共同第一作者。
基金资助:
河北省重点研发计划项目(19226424D);河北省现代农业产业技术体系草业创新团队建设专项(HBCT2018160201);张家口市科技支持计划项目(1511063C);张家口市重点研发计划项目(1911016C-2)

Response of Rhizosphere Soil Bacterial Community Diversity to Salt Stress in Oat （Avena sativa L.）

Feng LI¹(), Congpei YIN²(), Ran YIN³, Fan WANG², Yongliang HAN⁴, Zhimin YANG¹(), Jiancheng LIU¹()

^1.Zhangjiakou Academy of Agricultural Sciences，Hebei Zhangjiakou 075000，China
^2.College of Agronomy，Hebei Agricultural University，Hebei Baoding 071000，China
^3.College of Plant Protection，Hebei Agricultural University，Hebei Baoding 071000，China
^4.Handan Academy of Agriculture Science，Hebei Handan 056000，China

Received:2021-10-25 Accepted:2022-03-08 Online:2023-01-15 Published:2023-04-17
Contact: Zhimin YANG,Jiancheng LIU

摘要/Abstract

摘要：

为探究燕麦（Avena sativa L.）在不同盐胁迫处理下根际土壤微生物的群落多样性及变化特征，选取领袖（Souris，S）和爱沃（Everleaf，E）2个燕麦品种为试验材料，分别设置0（Y0）、3（Y1）、6（Y2）和9（Y3） g?kg^-1 NaCl胁迫处理。采用高通量测序技术对不同盐胁迫处理下燕麦根际土壤微生物进行测序，分析微生物群落多样性及其与土壤盐胁迫的相关性。结果表明，2个燕麦品种根际土壤细菌群落的多样性在4种盐胁迫处理下存在差异，其中S-Y2的群落丰度最高，S-Y3的多样性最丰富。在不同分类水平上，细菌变形菌门（Proteobacteria）、放线菌纲（Actinobacteria）、根瘤菌目（Rhizobiales）、norank_c_Subgroup_6科和norank_c_Subgroup_6属的相对丰度较高。盐胁迫影响燕麦根际微生物的群落组成，随着盐胁迫程度的增加，其菌落组成的相对丰度发生显著变化。通过对操作分类单元（OTU）丰度的16S功能预测和COG、KEGG代谢通路数据库对比发现，燕麦根际微生物的功能富集于新陈代谢、遗传信息处理、环境信息处理、细胞过程和生物体系统等。由此表明，根际微生物在燕麦生长和响应盐胁迫中起重要作用，为进一步挖掘和利用根际功能微生物奠定了理论基础。

关键词: 燕麦, 根际, 16S, 微生物群落结构, 盐胁迫

Abstract:

To investigate the diversity of microbial communities in the rhizosphere of oats （Avena sativa L.） under salt stress， the microbial communities in rhizosphere soils of two oat varieties Souris （S） and Everleaf （E） treated with 0 （Y0）， 3 （Y1）， 6 （Y2） and 9 g?kg^-1 NaCl （Y3） were sequenced using high-throughput sequencing technology. And the relationship between changes in microbial community diversity and salt stress in oats was further analyzed. The results showed that there were significant differences among the bacterial community diversity of different salt stress treatments， and the bacterial community abnudance of S-Y2 and the diversity of S-Y3 samples were highest. At the different classification level， the relative abundances of Proteobacteria， Actinobacteria， Rhizobiales， norank_c_Subgroup_6 and norank_c_Subgroup_6 were higher in the bacterial community. Salt stress affected the community composition of oat rhizosphere microorganisms， and the relative abundances changed significantly with the increase of salt stress. Through 16S functional prediction， COG metabolic pathway database and KEGG metabolic pathway database， it was found that the functions of oat rhizosphere microorganisms were enriched in metabolism， genetic information processing， environmental information processing， cellular processes and organismal systems. These functions of the rhizosphere microbial community were more active under salt stress， which played an important role in growth and stress response of oats. These results provided new space for the isolation of species an strains， and laid a theoretical basis for further mining and utilization of rhizosphere functional microbe.

Key words: oat, rhizosphere, 16S, microbial community structure, salt stress

中图分类号:

S512.6

李峰, 殷丛培, 殷冉, 王凡, 韩永亮, 杨志敏, 刘建成. 燕麦根际土壤细菌多样性对盐胁迫的响应[J]. 中国农业科技导报, 2023, 25(1): 153-165.

Feng LI, Congpei YIN, Ran YIN, Fan WANG, Yongliang HAN, Zhimin YANG, Jiancheng LIU. Response of Rhizosphere Soil Bacterial Community Diversity to Salt Stress in Oat （Avena sativa L.）[J]. Journal of Agricultural Science and Technology, 2023, 25(1): 153-165.

图/表 8

表1 燕麦根际细菌群落测序质量

Table 1 Sequencing quality of rhizosphere bacteria in oat

样本编号 Sample ID	有效序列数 Seq num	碱基数 Base num	序列平均长度 Mean length/bp	最短序列长度 Min length/bp	最长序列长度 Max length/bp
S-Y0	162 093	67 727 026	417.82	252.00	506.33
S-Y1	160 893	67 266 875	418.08	244.33	491.67
S-Y2	195 869	81 822 543	417.74	214.00	475.33
S-Y3	172 634	72 184 160	418.14	247.67	486.33
E-Y0	155 935	65 237 362	418.36	241.33	482.00
E-Y1	146 417	61 178 315	417.80	250.67	499.00
E-Y2	176 735	73 796 761	417.56	257.67	476.33
E-Y3	176 596	73 524 218	416.35	240.67	488.67

图 1 不同盐胁迫燕麦根际土壤细菌群落组成A：稀释曲线；B：燕麦根际土壤细菌Sobs指数；C：不同样品中 OTUs 数目花瓣图；***表示在P<0.001水平显著。

Fig. 1 Bacterial composition of oats rhizosphere soil samples under different salt stressA： Sparse curves； B： Sobs indices of oat rhizosphere soil； C： Flower plot analysis for bacterial species （OTUs） of different samples； *** represents significant differences at P<0.001 level.

图2 不同盐胁迫下燕麦根际土壤细菌的Alpha多样性

Fig. 2 Alpha diversity index of bacterial in oats rhizosphere soil under different salt stress

图3 不同盐胁迫下燕麦根际土壤细菌的β多样性A：OTU水平NMDS分析；B：属水平PCoA分析；C：不同品种分组；D：盐胁迫程度分组

Fig. 3 Beta diversity of bacterial in oats rhizosphere soil under different salt stressA： NMDS on OTU level； B： PCoA on genus level； C： Groups of different varieties； D： Groups of different treatment

图4 盐胁迫下燕麦根际土壤细菌群落结构A：门水平；B：纲水平；C：目水平；D：科水平

Fig. 4 Bacterial community abundance in oat rhizosphere soil under salt stressA： Phylum level； B： Class level； C： Order level； D： Family level

图5 不同盐处理下2个燕麦品种根际细菌属分类水平的相对丰度A：Y0；B：Y1；C：Y2；D：Y3；采用fdr法校正P值（P<0.05）。

Fig. 5 Relative abundance （genera level） of bacteria in rhizosphere soil of two oat varieties under different salt treatmentsA： Y0；B： Y1；C： Y2；D： Y3； corrected P-values are calculated by the fdr false discovery rate approach （P<0.05）.

图6 燕麦根际微生物的系统发生进化关系和功能预测A：燕麦根际微生物的系统发生进化关系；B：燕麦根际微生物的一级和二级功能预测

Fig. 6 Evolutionary relationship and function prediction in rhizosphere soil bacterial of oatsA： Evolutionary relationship in rhizosphere soil of oats； B： Function prediction of oat rhizosphere bacterial COG analysis in level 1 and 2

图7 细菌系统发生树及菌群功能预测A：KEGG一级和二级代谢通路；B：KEGG三级代谢通路

Fig. 7 Bacterial phylogenetic tree and prediction of flora functionA： KEGG of level 1，2； B： KEGG of level 3

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燕麦根际土壤细菌多样性对盐胁迫的响应

Response of Rhizosphere Soil Bacterial Community Diversity to Salt Stress in Oat （Avena sativa L.）

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