糯玉米萌发期耐低温品种资源的综合评价

doi:10.13304/j.nykjdb.2022.0550

摘要/Abstract

摘要：

为构建不同糯玉米品种萌发期耐低温评价体系，以48个糯玉米品种为试验材料，测定低温胁迫下糯玉米的10个萌发及生长指标，通过主成分分析法、隶属函数法、聚类分析和回归分析，对糯玉米品种耐低温性进行综合评价与分类。结果表明，主成分分析将10个测定指标转化为根系生长因子和种子萌发因子2个相互独立的综合指标，建立了糯玉米耐低温综合得分模型Y=0.647 7Y₁+0.144 2Y₂（其中，Y₁、Y₂为2个主成分，Y₁代表的主要指标包括相对根干重、相对根表面积、相对根体积、相对根长，反映低温胁迫下糯玉米根系生长的状态；Y₂代表的主要指标包括相对苗干重、相对发芽率、相对活力指数、相对发芽势，反映低温胁迫下糯玉米的萌发情况）。通过隶属函数法对48个糯玉米品种的耐低温能力进行排序，筛选出5个耐低温能力较强的品种，依次为‘润彩糯686’‘景坡82’‘润糯175’‘润糯605’和‘天贵糯932’。聚类分析将48个糯玉米品种划分成4个类群。回归分析表明，相对根干重、相对苗长、相对发芽势、相对发芽率、相对苗干重和相对根表面积可作为糯玉米耐低温评价的重要指标。多方法关联分析的综合评价方法可对不同耐低温能力的糯玉米品种进行有效筛选分类，为糯玉米耐低温评价及耐低温种质资源的筛选提供参考。

关键词: 糯玉米, 低温胁迫, 主成分分析, 隶属函数法

Abstract:

In order to construct evaluation system of different waxy maize varieties during germination for low temperature tolerance， 48 waxy maize varieties were used as test materials to measure 10 indicators on germination and growth under low temperature stress. And the low temperature tolerances of waxy maize varieties were comprehensively evaluated by principal component analysis， membership function method， cluster analysis and regression analysis. The results showed that 10 measurement indexes were transformed into 2 independent comprehensive indexes by principal component analysis， including root growth factor and seed germination factor. The comprehensive score model of low temperature tolerance of waxy maize was established， Y=0.647 7Y₁+0.144 2Y₂， where Y₁ and Y₂ were scores of the two principal components， respectively. Y₁ included relative root dry weight， relative root surface area， relative root volume， and relative root length， which could reflect the state of root growth of waxy maize under low temperature stress； and Y₂ included relative seedling dry weight， relative germination rate， relative vitality index， and relative germination potential， which could reflect the germination of waxy maize under low temperature stress. The 48 waxy maize varieties were ranked by affiliation function method for low temperature tolerance， and the five varieties with the strong low temperature resistance were screened， including ‘Runcainuo 686’‘ Jingpo 82’‘Runnuo 175’‘Runnuo 605’ and ‘Tianguinuo 932’. The cluster analysis classified 48 waxy maize varieties into 4 groups. The regression analysis showed that the relative root dry weight， relative seedling length， relative germination potential， relative germination rate， relative seedling dry weight and relative root surface area could be used as important indicators for the evaluation of low-temperature tolerance of waxy maize. The comprehensive evaluation of multiple methods could effectively screen and classify waxy maize varieties with different low-temperature tolerances， which provided reference for the evaluation of waxy maize low-temperature tolerance and the screening of low-temperature tolerance germplasm resources.

Key words: waxy maize, chilling stress, principal component analysis, membership function method

中图分类号:

S513

郭胜微, 边思文, 丁建文, 张晓辰, 杨兴, 杜锦, 向春阳. 糯玉米萌发期耐低温品种资源的综合评价[J]. 中国农业科技导报, 2023, 25(2): 38-47.

Shengwei GUO, Siwen BIAN, Jianwen DING, Xiaochen ZHANG, Xing YANG, Jin DU, Chunyang XIANG. Comprehensive Evaluation of Low Temperature Tolerance of Waxy Maize Varieties at Germination Stage[J]. Journal of Agricultural Science and Technology, 2023, 25(2): 38-47.

图/表 6

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编号Code	品种名称Name of variety	编号 Code	品种名称Name of variety	编号 Code	品种名称Name of variety	编号 Code	品种名称Name of variety
1	京科糯2000 Jingkenuo 2000	13	万糯2000 Wannuo 2000	25	润糯175 Runnuo175	37	天贵糯932 Tianguinuo 932
2	景坡白糯 Jingpobainuo	14	景科糯1号 Jingkenuo 1	26	润甜糯760 Runtiannuo 760	38	润糯73 Runnuo 73
3	农科玉336 Nongkeyu 336	15	润黑糯729 Runheinuo 729	27	润甜糯618 Runtiannuo 618	39	润甜糯828 Runtiannuo 828
4	津糯215 Jinnuo 215	16	彩甜糯6号 Caitiannuo 6	28	景糯307 Jingnuo 307	40	大黄粘 Dahuangnian
5	润黑糯829 Runheinuo 829	17	景坡82 Jingpo 82	29	景彩甜糯2128 Jingcaitiannuo 2128	41	润甜糯876 Runtiannuo 876
6	润糯72 Runnuo 72	18	润黑甜糯966 Runheitiannuo 966	30	润糯605 Runnuo 605	42	华耐甜糯358 Huanaitiannuo 358
7	润糯163 Runnuo 163	19	润黑糯529 Runheinuo 529	31	景颇早18 Jingpozao 18	43	栗香红糯 Lixianghongnuo
8	润彩糯686 Runcainuo 686	20	津糯72 Jinnuo 72	32	景颇556 Jingpo 556	44	津糯1+1 Jinnuo 1+1
9	津糯69 Jinnuo 69	21	景坡银糯 Jingpoyinnuo	33	润糯597 Runnuo 597	45	润糯863 Runnuo 863
10	润糯1704 Runnuo 1704	22	润甜糯907 Runtiannuo 907	34	润糯615 Runnuo 615	46	黄糯早53 Huangnuozao 53
11	润糯622 Runnuo 622	23	润黑糯629 Runheinuo 629	35	景颇早糯 Jingpozaonuo	47	润糯197 Runnuo 197
12	润甜糯70 Runtiannuo 70	24	景甜糯1248 Jingtiannuo 1248	36	津糯711 Jinnuo 711	48	润糯650 Runnuo 650

编号Code	品种名称Name of variety	编号 Code	品种名称Name of variety	编号 Code	品种名称Name of variety	编号 Code	品种名称Name of variety
1	京科糯2000 Jingkenuo 2000	13	万糯2000 Wannuo 2000	25	润糯175 Runnuo175	37	天贵糯932 Tianguinuo 932
2	景坡白糯 Jingpobainuo	14	景科糯1号 Jingkenuo 1	26	润甜糯760 Runtiannuo 760	38	润糯73 Runnuo 73
3	农科玉336 Nongkeyu 336	15	润黑糯729 Runheinuo 729	27	润甜糯618 Runtiannuo 618	39	润甜糯828 Runtiannuo 828
4	津糯215 Jinnuo 215	16	彩甜糯6号 Caitiannuo 6	28	景糯307 Jingnuo 307	40	大黄粘 Dahuangnian
5	润黑糯829 Runheinuo 829	17	景坡82 Jingpo 82	29	景彩甜糯2128 Jingcaitiannuo 2128	41	润甜糯876 Runtiannuo 876
6	润糯72 Runnuo 72	18	润黑甜糯966 Runheitiannuo 966	30	润糯605 Runnuo 605	42	华耐甜糯358 Huanaitiannuo 358
7	润糯163 Runnuo 163	19	润黑糯529 Runheinuo 529	31	景颇早18 Jingpozao 18	43	栗香红糯 Lixianghongnuo
8	润彩糯686 Runcainuo 686	20	津糯72 Jinnuo 72	32	景颇556 Jingpo 556	44	津糯1+1 Jinnuo 1+1
9	津糯69 Jinnuo 69	21	景坡银糯 Jingpoyinnuo	33	润糯597 Runnuo 597	45	润糯863 Runnuo 863
10	润糯1704 Runnuo 1704	22	润甜糯907 Runtiannuo 907	34	润糯615 Runnuo 615	46	黄糯早53 Huangnuozao 53
11	润糯622 Runnuo 622	23	润黑糯629 Runheinuo 629	35	景颇早糯 Jingpozaonuo	47	润糯197 Runnuo 197
12	润甜糯70 Runtiannuo 70	24	景甜糯1248 Jingtiannuo 1248	36	津糯711 Jinnuo 711	48	润糯650 Runnuo 650

指标Index	第1主成分PC 1	第2主成分PC 2
相对发芽势RGE	0.227	0.309
相对发芽率RGR	0.268	0.374
相对活力指数RVI	0.328	0.372
相对苗长RSL	0.340	-0.025
相对根长RRL	0.350	-0.314
相对根表面积RRSA	0.358	-0.309
相对根体积RRV	0.351	-0.284
相对苗干重RSDW	0.253	0.463
相对根干重RRDW	0.363	0.067
相对根系数RRN	0.290	-0.363
特征值Eigenvalue	6.477	1.442
贡献率Contribution rate/%	64.766	14.417
累计贡献率Cumulative contribution rate/%	64.766	79.183

指标Index	第1主成分PC 1	第2主成分PC 2
相对发芽势RGE	0.227	0.309
相对发芽率RGR	0.268	0.374
相对活力指数RVI	0.328	0.372
相对苗长RSL	0.340	-0.025
相对根长RRL	0.350	-0.314
相对根表面积RRSA	0.358	-0.309
相对根体积RRV	0.351	-0.284
相对苗干重RSDW	0.253	0.463
相对根干重RRDW	0.363	0.067
相对根系数RRN	0.290	-0.363
特征值Eigenvalue	6.477	1.442
贡献率Contribution rate/%	64.766	14.417
累计贡献率Cumulative contribution rate/%	64.766	79.183

品种 Variety	Y₁	Y₂	U₁	U₂	D值 D value	排名Ranking
京科糯2000 Jingkenuo 2000	-1.578	-0.396	0.336	0.623	0.388	36
景坡白糯 Jingpobainuo	0.842	0.007	0.544	0.682	0.569	19
农科玉336 Nongkeyu 336	2.077	-0.046	0.650	0.674	0.654	10
津糯215 Jinnuo 215	-1.192	-1.566	0.369	0.450	0.384	38
润黑糯829 Runheinuo 829	2.625	-0.620	0.697	0.589	0.677	8
润糯72 Runnuo 72	0.903	-0.757	0.549	0.569	0.553	20
润糯163 Runnuo 163	-2.603	-1.186	0.249	0.506	0.295	43
润彩糯686 Runcainuo 686	6.167	-0.159	1.000	0.658	0.938	1
津糯69 Jinnuo 69	-3.697	-0.831	0.155	0.558	0.228	46
润糯1704 Runnuo 1704	-2.757	0.238	0.235	0.716	0.323	40
润糯622 Runnuo 622	2.830	-4.612	0.714	0.000	0.584	15
润甜糯70 Runtiannuo 70	-0.107	0.138	0.462	0.701	0.506	26
万糯2000 Wannuo 2000	1.538	1.191	0.603	0.857	0.650	11
景科糯1号 Jingkenuo 1	-0.363	1.383	0.440	0.885	0.521	24
润黑糯729 Runheinuo 729	2.066	1.090	0.649	0.842	0.684	7
彩甜糯6号 Caitiannuo 6	-0.887	0.816	0.396	0.801	0.469	31
景坡82 Jingpo 82	4.599	2.160	0.866	1.000	0.890	2
润黑甜糯966 Runheitiannuo 966	0.951	-0.173	0.553	0.655	0.572	17
润黑糯529 Runheinuo 529	1.147	0.560	0.570	0.764	0.605	13
津糯72 Jinnuo 72	-5.295	0.450	0.018	0.747	0.151	47
景坡银糯 Jingpoyinnuo	0.005	1.013	0.472	0.831	0.537	22
润甜糯907 Runtiannuo 907	-2.674	-0.246	0.242	0.645	0.316	41
润黑糯629 Runheinuo 629	1.673	-1.838	0.615	0.410	0.578	16
景甜糯1248 Jingtiannuo 1248	-2.268	0.180	0.277	0.708	0.356	39
润糯175 Runnuo 175	4.582	-0.397	0.864	0.622	0.820	3
润甜糯760 Runtiannuo 760	1.870	-0.080	0.632	0.669	0.639	12
润甜糯618 Runtiannuo 618	-1.436	-0.317	0.349	0.634	0.401	34
景糯307 Jingnuo 307	2.089	1.986	0.651	0.974	0.709	6
景彩甜糯2128 Jingcaitiannuo 2128	-1.162	0.666	0.372	0.779	0.446	32
润糯605 Runnuo 605	3.580	0.957	0.778	0.822	0.786	4
景颇早18 Jingpozao 18	1.396	1.926	0.591	0.965	0.659	9
景颇556 Jingpo 556	-1.047	1.279	0.382	0.870	0.471	30
润糯597 Runnuo 597	-5.504	0.139	0.000	0.702	0.128	48
润糯615 Runnuo 615	2.039	-2.376	0.646	0.330	0.589	14
景颇早糯 Jingpozaonuo	0.671	0.524	0.529	0.758	0.571	18
津糯711 Jinnuo 711	-3.148	-1.248	0.202	0.497	0.256	44
天贵糯932 Tianguinuo 932	3.642	-0.307	0.784	0.636	0.757	5
润糯73 Runnuo 73	-0.328	0.155	0.443	0.704	0.491	28
润甜糯828 Runtiannuo 828	0.121	-0.478	0.482	0.611	0.505	27
大黄粘 Dahuangnian	0.082	0.924	0.479	0.818	0.540	21
润甜糯876 Runtiannuo 876	-1.368	-1.076	0.354	0.522	0.385	37
华耐甜糯358 Huanaitiannuo 358	-4.567	1.809	0.080	0.948	0.238	45
栗香红糯 Lixianghongnuo	0.168	0.122	0.486	0.699	0.525	23
津糯1+1 Jinnuo 1+1	0.080	0.216	0.478	0.713	0.521	25
润糯863 Runnuo 863	-1.301	-0.928	0.360	0.544	0.394	35
黄糯早53 Huangnuozao 53	-1.316	0.111	0.359	0.697	0.420	33
润糯197 Runnuo 197	-0.535	0.419	0.426	0.743	0.483	29
润糯650 Runnuo 650	-2.609	-0.822	0.248	0.560	0.305	42
权重Weight			0.818	0.182