中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (6): 64-71.DOI: 10.13304/j.nykjdb.2023.0797
邹声浩1(), 喻奇伟2(
), 贺帅2, 张学伟3, 马黔2, 马关凯2, 席飞虎1, 罗东升1, 王茂贤2(
), 罗贞宝2(
), 景延秋1(
)
收稿日期:
2023-11-01
接受日期:
2024-01-09
出版日期:
2025-06-15
发布日期:
2025-06-23
通讯作者:
王茂贤,罗贞宝,景延秋
作者简介:
邹声浩 E-mail:3235271728@qq.com基金资助:
Shenghao ZOU1(), Qiwei YU2(
), Shuai HE2, Xuewei ZHANG3, Qian MA2, Guankai MA2, Feihu XI1, Dongsheng LUO1, Maoxian WANG2(
), Zhenbao LUO2(
), Yanqiu JING1(
)
Received:
2023-11-01
Accepted:
2024-01-09
Online:
2025-06-15
Published:
2025-06-23
Contact:
Maoxian WANG,Zhenbao LUO,Yanqiu JING
摘要:
为研究外源小球藻对干旱胁迫下烤烟幼苗生理特性的影响,以烤烟品种‘中烟100’为试验材料,以喷施清水为对照(CK),分别设置干旱处理(D)及在干旱处理的基础上将小球藻稀释50(T1)、100(T2)、150倍(T3)后施用于烤烟幼苗,对不同处理下烤烟幼苗的生理特性进行比较。结果表明,施用小球藻后,烤烟幼苗的抗氧化酶活性、光合特性及可溶性蛋白和叶绿素含量均得到提升,丙二醛和脯氨酸含量下降,其中T1处理对于缓解干旱胁迫对烤烟的生长发育的效果最佳。综上,施用小球藻能有效缓解干旱胁迫对烤烟幼苗的影响。
中图分类号:
邹声浩, 喻奇伟, 贺帅, 张学伟, 马黔, 马关凯, 席飞虎, 罗东升, 王茂贤, 罗贞宝, 景延秋. 外源小球藻对干旱胁迫下烤烟幼苗生理特性的影响[J]. 中国农业科技导报, 2025, 27(6): 64-71.
Shenghao ZOU, Qiwei YU, Shuai HE, Xuewei ZHANG, Qian MA, Guankai MA, Feihu XI, Dongsheng LUO, Maoxian WANG, Zhenbao LUO, Yanqiu JING. Effect of Exogenous Chlorellavulgaris on Physiological Characteristics of Flue-cured Tobacco Seedlings Under Drought Stress[J]. Journal of Agricultural Science and Technology, 2025, 27(6): 64-71.
处理 Treatment | 土壤平均含水量 Average soil moisture content/% | 小球藻含量 Content of Chlorella vulgaris/(mg·L-1) |
---|---|---|
CK | 43 | — |
D | 21 | — |
T1 | 21 | 79.40±0.29 |
T2 | 21 | 38.60±3.13 |
T3 | 21 | 24.48±1.62 |
表1 试验处理及方法
Table 1 Test processing and methods
处理 Treatment | 土壤平均含水量 Average soil moisture content/% | 小球藻含量 Content of Chlorella vulgaris/(mg·L-1) |
---|---|---|
CK | 43 | — |
D | 21 | — |
T1 | 21 | 79.40±0.29 |
T2 | 21 | 38.60±3.13 |
T3 | 21 | 24.48±1.62 |
图1 不同处理下烤烟叶片的抗氧化酶活性注:不同小写字母表示同一时间不同处理间在P<0.05水平差异显著。
Fig. 1 Activity of antioxidant enzymes in flue-cured tobacco leaves under different treatmentsNote:Different lowercase letters indicate significant differences between different treatments of same time at P<0.05 level.
图2 不同处理下烤烟的丙二醛含量注:不同小写字母表示同一时间不同处理间在P<0.05水平差异显著。
Fig. 2 MDA content of flue-cured tobacco under different treatmentsNote: Different lowercase letters indicate significant differences between different treatments of same time at P<0.05 level.
图3 不同处理下烤烟的根系活力注:不同小写字母表示同一时间不同处理间在P<0.05水平差异显著。
Fig. 3 Root vitality of flue-cured tobacco under different treatmentsNote:Different lowercase letters indicate significant differences between different treatments of same time at P<0.05 level.
图4 不同处理下烤烟渗透调节物质含量注:不同小写字母表示同一时间不同处理间在P<0.05水平差异显著。
Fig. 4 Contents of osmotic regulating substances in flue-cured tobacco under different treatmentsNote: Different lowercase letters indicate significant differences between different treatments of same time at P<0.05 level.
胁迫时间Stress time/d | 处理 Treatment | 净光合速率Pn/ (μmol CO2·m-2·s-1) | 气孔导度Gs/ (mmol·m-2·s-1) | 胞间CO2浓度Ci/ (μL·L-1) | 蒸腾速率Tr/ (mmol·m-2·s-1) |
---|---|---|---|---|---|
0 | CK | 12.68±0.95 a | 0.38±0.26 a | 146.63±4.13 b | 4.36±1.17 bc |
D | 11.41±0.68 a | 0.37±0.03 a | 149.75±14.73 a | 4.53±0.59 b | |
T1 | 11.58±1.06 a | 0.33±0.12 a | 141.43±28.54 c | 4.72±1.36 a | |
T2 | 13.31±3.13 a | 0.36±0.24 a | 139.76±19.48 cd | 4.23±1.73 c | |
T3 | 11.93±1.47 a | 0.35±0.07 a | 139.47±18.09 d | 4.47±1.51 b | |
5 | CK | 16.13±0.45 a | 0.65±0.05 a | 151.27±30.55 e | 6.12±0.87 a |
D | 8.03±0.58 d | 0.57±0.01 b | 211.06±25.63 a | 5.59±0.49 b | |
T1 | 12.04±3.63 b | 0.32±0.12 c | 175.45±14.95 d | 4.63±1.84 b | |
T2 | 10.67±0.85 bc | 0.36±0.08 c | 184.53±15.71 c | 3.86±0.83 c | |
T3 | 9.53±2.86 cd | 0.41±0.05 c | 196.45±22.81 b | 3.49±1.04 d | |
10 | CK | 19.56±2.41 a | 0.89±0.06 a | 175.92±26.55 e | 9.23±2.66 a |
D | 11.28±1.09 d | 0.64±0.02 b | 243.84±12.82 a | 7.58±0.85 b | |
T1 | 16.22±1.52 b | 0.53±0.14 c | 197.73±61.48 d | 7.46±2.81 b | |
T2 | 14.48±0.65 c | 0.60±0.23 bc | 213.80±30.46 c | 6.85±0.93 c | |
T3 | 13.83±3.40 c | 0.69±0.18 b | 226.12±20.83 b | 6.53±1.06 d | |
15 | CK | 23.43±0.72 a | 1.03±0.26 a | 229.06±34.61 e | 12.88±2.69 a |
D | 14.52±1.02 d | 0.85±0.01 ab | 286.49±16.49 a | 9.69±0.52 b | |
T1 | 20.20±2.58 b | 0.74±0.23 b | 239.15±19.68 d | 8.36±1.75 bc | |
T2 | 18.81±2.96 bc | 0.79±0.20 b | 254.19±25.05 c | 7.93±0.91 bc | |
T3 | 17.23±1.28 c | 0.86±0.17 ab | 266.81±37.51 b | 7.24±3.15 c |
表2 不同处理下烤烟的光合特性
Table 2 Photosynthetic characteristics of flue-cured tobacco under different treatments
胁迫时间Stress time/d | 处理 Treatment | 净光合速率Pn/ (μmol CO2·m-2·s-1) | 气孔导度Gs/ (mmol·m-2·s-1) | 胞间CO2浓度Ci/ (μL·L-1) | 蒸腾速率Tr/ (mmol·m-2·s-1) |
---|---|---|---|---|---|
0 | CK | 12.68±0.95 a | 0.38±0.26 a | 146.63±4.13 b | 4.36±1.17 bc |
D | 11.41±0.68 a | 0.37±0.03 a | 149.75±14.73 a | 4.53±0.59 b | |
T1 | 11.58±1.06 a | 0.33±0.12 a | 141.43±28.54 c | 4.72±1.36 a | |
T2 | 13.31±3.13 a | 0.36±0.24 a | 139.76±19.48 cd | 4.23±1.73 c | |
T3 | 11.93±1.47 a | 0.35±0.07 a | 139.47±18.09 d | 4.47±1.51 b | |
5 | CK | 16.13±0.45 a | 0.65±0.05 a | 151.27±30.55 e | 6.12±0.87 a |
D | 8.03±0.58 d | 0.57±0.01 b | 211.06±25.63 a | 5.59±0.49 b | |
T1 | 12.04±3.63 b | 0.32±0.12 c | 175.45±14.95 d | 4.63±1.84 b | |
T2 | 10.67±0.85 bc | 0.36±0.08 c | 184.53±15.71 c | 3.86±0.83 c | |
T3 | 9.53±2.86 cd | 0.41±0.05 c | 196.45±22.81 b | 3.49±1.04 d | |
10 | CK | 19.56±2.41 a | 0.89±0.06 a | 175.92±26.55 e | 9.23±2.66 a |
D | 11.28±1.09 d | 0.64±0.02 b | 243.84±12.82 a | 7.58±0.85 b | |
T1 | 16.22±1.52 b | 0.53±0.14 c | 197.73±61.48 d | 7.46±2.81 b | |
T2 | 14.48±0.65 c | 0.60±0.23 bc | 213.80±30.46 c | 6.85±0.93 c | |
T3 | 13.83±3.40 c | 0.69±0.18 b | 226.12±20.83 b | 6.53±1.06 d | |
15 | CK | 23.43±0.72 a | 1.03±0.26 a | 229.06±34.61 e | 12.88±2.69 a |
D | 14.52±1.02 d | 0.85±0.01 ab | 286.49±16.49 a | 9.69±0.52 b | |
T1 | 20.20±2.58 b | 0.74±0.23 b | 239.15±19.68 d | 8.36±1.75 bc | |
T2 | 18.81±2.96 bc | 0.79±0.20 b | 254.19±25.05 c | 7.93±0.91 bc | |
T3 | 17.23±1.28 c | 0.86±0.17 ab | 266.81±37.51 b | 7.24±3.15 c |
胁迫时间 Stress time/d | 处理 Treatment | 叶绿素a含量 Chl a content/(mg·g-1) | 叶绿素b含量 Chl b content/(mg·g-1) | 类胡萝卜素含量 Car content/(mg·g-1) |
---|---|---|---|---|
0 | CK | 1.74±0.51 a | 0.67±0.15 a | 0.39±0.07 a |
D | 1.03±0.68 c | 0.31±0.03 b | 0.20±0.03 a | |
T1 | 1.12±0.39 c | 0.37±0.07 b | 0.22±0.05 a | |
T2 | 1.38±0.31 b | 0.44±0.12 b | 0.26±0.02 a | |
T3 | 1.54±0.43 b | 0.49±0.17 ab | 0.29±0.04 a | |
5 | CK | 4.91±0.93 a | 1.28±0.56 a | 0.68±0.07 a |
D | 2.87±0.67 d | 0.83±0.56 b | 0.41±0.10 b | |
T1 | 3.55±0.78 b | 0.88±0.41 b | 0.57±0.12 ab | |
T2 | 3.26±1.13 c | 0.97±0.33 b | 0.50±0.08 ab | |
T3 | 3.03±1.05 d | 1.15±0.28 a | 0.45±0.06 b | |
10 | CK | 7.35±1.86 a | 2.95±1.04 a | 0.87±0.32 a |
D | 5.17±2.04 e | 1.56±0.38 e | 0.55±0.09 bc | |
T1 | 6.22±1.31 b | 2.53±0.43 b | 0.71±0.06 ab | |
T2 | 5.98±1.67 c | 2.26±0.52 c | 0.66±0.23 bc | |
T3 | 5.61±1.74 d | 1.89±0.24 d | 0.52±0.14 c | |
15 | CK | 9.01±1.58 a | 3.83±1.21 a | 1.60±0.48 a |
D | 7.23±1.52 d | 2.67±0.36 d | 1.08±0.37 d | |
T1 | 7.92±2.07 b | 3.25±1.15 b | 1.39±0.25 b | |
T2 | 7.67±1.39 c | 3.03±1.09 c | 1.26±0.35 bc | |
T3 | 7.21±2.12 d | 2.83±0.57 d | 1.17±0.19 cd |
表3 不同处理下烤烟的叶绿素含量
Table 3 Chlorophyll content of flue-cured tobacco under different treatments
胁迫时间 Stress time/d | 处理 Treatment | 叶绿素a含量 Chl a content/(mg·g-1) | 叶绿素b含量 Chl b content/(mg·g-1) | 类胡萝卜素含量 Car content/(mg·g-1) |
---|---|---|---|---|
0 | CK | 1.74±0.51 a | 0.67±0.15 a | 0.39±0.07 a |
D | 1.03±0.68 c | 0.31±0.03 b | 0.20±0.03 a | |
T1 | 1.12±0.39 c | 0.37±0.07 b | 0.22±0.05 a | |
T2 | 1.38±0.31 b | 0.44±0.12 b | 0.26±0.02 a | |
T3 | 1.54±0.43 b | 0.49±0.17 ab | 0.29±0.04 a | |
5 | CK | 4.91±0.93 a | 1.28±0.56 a | 0.68±0.07 a |
D | 2.87±0.67 d | 0.83±0.56 b | 0.41±0.10 b | |
T1 | 3.55±0.78 b | 0.88±0.41 b | 0.57±0.12 ab | |
T2 | 3.26±1.13 c | 0.97±0.33 b | 0.50±0.08 ab | |
T3 | 3.03±1.05 d | 1.15±0.28 a | 0.45±0.06 b | |
10 | CK | 7.35±1.86 a | 2.95±1.04 a | 0.87±0.32 a |
D | 5.17±2.04 e | 1.56±0.38 e | 0.55±0.09 bc | |
T1 | 6.22±1.31 b | 2.53±0.43 b | 0.71±0.06 ab | |
T2 | 5.98±1.67 c | 2.26±0.52 c | 0.66±0.23 bc | |
T3 | 5.61±1.74 d | 1.89±0.24 d | 0.52±0.14 c | |
15 | CK | 9.01±1.58 a | 3.83±1.21 a | 1.60±0.48 a |
D | 7.23±1.52 d | 2.67±0.36 d | 1.08±0.37 d | |
T1 | 7.92±2.07 b | 3.25±1.15 b | 1.39±0.25 b | |
T2 | 7.67±1.39 c | 3.03±1.09 c | 1.26±0.35 bc | |
T3 | 7.21±2.12 d | 2.83±0.57 d | 1.17±0.19 cd |
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