Abstract:

In order to screen out microalgae, which was fast growing with high oil productivity, 40 strains were isolated from the municipal waste-water. An excellent microalga was screened by high-throughput method (cultured in 96-well plates and stained by Nile red). The isolated strain was identified as Chlorella sp. assisted by both 18S rDNA sequencing and morphological observation. After sewage cultivation for 7 d, its lipid content was 31.21%, and the removal efficiencies of total nitrogen, ammonia nitrogen, total phosphorus and COD were 71.6%, 98.2%, 99.4%, 78.8%, respectively. This microalga had the potential of application prospect in the coupling system of sewage treatment with micro-algae bioenergy production.

Key words: microalgal isolate, identification, lipid content, wastewater treatment

摘要：

为筛选在城市生活污水中生长快、产油高的微藻，从污水样品中分离出40株微藻，采用高通量方法（96孔板培养、尼罗红染色）从中筛选出一株优良藻株，经18S rRNA基因序列分析及形态学鉴定属于小球藻属（Chlorella）。污水培养7 d后，其油脂含量达31.21%，污水的TN、NH+4-N、TP和COD去除率分别为71.6%、98.2%、99.4%和78.8%。该藻株在污水处理与微藻生物能源生产耦合系统中具有潜在的应用前景。

关键词: 微藻分离；鉴定；油脂含量；污水处理