Abstract: This paper determined the detoxifying enzyme and acetyl cholinesterase (AChE) activity of Myzus persicae after feeding on ginsenoside by leaf impregnation method, and compared the effects of Rg1 and Rb1 on the enzyme activity  by using ginsenoside Rg1 and Rb1 as the experimental materials and M. persicae as the experimental insect. The results showed that: Rg1 and Rb1 significantly inhibited the feeding of M. persicae, and the detoxifying enzyme activity of M. persicae at a lower concentration. After treatment for 24 h, Rg1 significantly inhibited glutathione -S- transferase (GSTs), multi-function oxidase (MFO), carboxylesterase (CarE) and AChE. After treatment for 48 h, Rb1 significantly inhibited the MFO, CarE and AChE of M. persicae. Moreover, the inhibition of Rg1 on detoxifying enzyme of M. persicae was more significant than that of Rb1; Rg1 and Rb1 could induce the enhancement of enzyme activity and break the balance of detoxifying enzyme activity of M. persicae. These results could provide reference for biological control of plant pests and the development of new pesticides.

Key words: ginsenoside, Rg1, Rb1, Myzus persicae, enzyme activity

摘要： 以人参单体皂苷Rg1和Rb1为试验材料，以同翅目桃蚜为试验昆虫，测定了桃蚜取食单体皂苷后解毒酶及乙酰胆碱酯酶活性，比较了Rg1和Rb1对酶活性的影响。结果表明：Rg1和Rb1对桃蚜取食有显著抑制作用，在较低浓度时对桃蚜体内解毒酶活性有抑制作用。处理24 h后，Rg1对桃蚜体内谷胱甘肽-S-转移酶、多功能氧化酶、羧酸酯酶、乙酰胆碱酯酶有显著抑制作用。处理48 h后，Rb1对桃蚜体内的多功能氧化酶、羧酸酯酶、乙酰胆碱酯酶有明显的抑制作用；且Rg1对桃蚜解毒酶的抑制作用比Rb1显著；Rg1和Rb1有诱导酶活性增强的作用，打破了桃蚜体内解毒酶活性均衡。研究结果可为植物害虫的生物防治及新农药开发提供参考。

关键词: 人参单体皂苷, Rg1, Rb1, 桃蚜, 酶活性