Effect of TS-PAA Water Retaining Agent on Growth and Photosynthetic Characteristics of Cigar under Drought Stress

doi:10.13304/j.nykjdb.2020.0970

Abstract

Abstract:

In order to explore the effects of the TS-PAA water-retaining agent on the growth and photosynthetic characteristics of cigar under drought stress， a pot experiment was carried out with Dexue 3 cigar as material， and 5 water-retaining agent dosages were set. The soil moisture content， cigar growth and development index， leaf active oxygen content， antioxidant enzyme activity and photosynthetic characteristics were measured under different dosages of water-retaining agents. The results were as followed： ① with the aggravation of drought stress， the application of this water-retaining agent could effectively increase soil moisture content， cigar root activity， dry/fresh weight of the shoots and roots of cigar； ②under drought stress， the application of TS-PAA water retaining agent increased the activity of superoxide dismutase （SOD）， peroxidase （POD）， catalase （CAT） and soluble protein content of cigar leaves， and decreased the content of malondialdehyde （MDA）， hydrogen peroxide （H₂O₂） and superoxide anion， but the effects were different with different dosages； ③the application of TS-PAA water retention agent under drought stress increased the photosynthetic pigment content and photosynthesis of cigar tobacco leaves， and the chlorophyll content， net photosynthetic rate （P_n）， transpiration rate （T_r）， stomatal conductance （G_s） and water use efficiency （WUE） increased firstly and then decreased with the increase of the amount of TS-PAA， while the intercellular CO₂ concentration （C_i） and foliar water pressure deficit （VPD） showed the opposite trend. Therefore， the application of TS-PAA water retaining agent enhanced drought stress tolerance of cigar under drought stress. In this study， the effect of water retaining agent was optimal at 0.24% （the ratio of water retaining agent quality to soil dry weight）.

Key words: drought stress, water retaining agent, cigar, growth and development, photosynthetic characteristics

摘要：

为探究TS-PAA保水剂对干旱胁迫下雪茄烟生长发育及光合特性的影响，以德雪三号雪茄烟为材料进行盆栽试验，测定了不同用量保水剂处理下的植烟土壤含水率、雪茄烟生长发育指标、叶片活性氧含量、抗逆性酶活性和光合特性。结果表明：①随着干旱胁迫程度加剧，施用TS-PAA保水剂能有效提高土壤含水率、雪茄烟根系活力和地上部及根系干鲜重；②干旱胁迫下，施用TS-PAA保水剂提高了雪茄烟叶片超氧化物歧化酶、过氧化物酶、过氧化氢酶活性和可溶性蛋白含量，降低了丙二醛、过氧化氢和超氧阴离子的含量，但不同用量作用效果不同；③干旱胁迫下施用TS-PAA保水剂提高了雪茄烟叶片光合色素含量和光合作用，叶绿素含量、净光合速率、蒸腾速率、气孔导度、水分利用效率都随着保水剂用量的增加呈现先增加后下降趋势，胞间CO₂浓度和叶面水气压亏缺变化趋势与之相反。因此，干旱胁迫下施用TS-PAA保水剂能增强雪茄烟干旱胁迫耐受性，以0.24%（保水剂质量与土壤干重的比例）的用量作用效果最佳。

关键词: 干旱胁迫, 保水剂, 雪茄烟, 生长发育, 光合特性

CLC Number:

S567

Fangling WANG, Mingyue ZHANG, Yaru ZHOU, Qinglin GUAN, Xinyan LI, Qiu ZHONG, Mingqin ZHAO. Effect of TS-PAA Water Retaining Agent on Growth and Photosynthetic Characteristics of Cigar under Drought Stress[J]. Journal of Agricultural Science and Technology, 2022, 24(4): 162-172.

王方玲, 张明月, 周亚茹, 管庆林, 李欣燕, 钟秋, 赵铭钦. 干旱胁迫下TS-PAA保水剂对雪茄烟生长发育和光合特性的影响[J]. 中国农业科技导报, 2022, 24(4): 162-172.

Figures/Tables 8

Fig.1 Effect of different dosages of water-retaining agent on soil moisture content under drought stressNote： Different small letters at the same treatment time indicate significant difference at P<0.05 level.

Fig.2 Effect of different dosages of water-retaining agent on cigar root activity under drought stressNote： Different small letters at the same treatment time indicate significant difference at P<0.05 level.

Fig.3 Effect of different dosages of water-retaining agent on the fresh and dry weights of the shoots and roots of cigar under drought stressNote： Different small letters at the same treatment time indicate significant difference at P<0.05 level.

Fig.4 Effect of different dosages of water-retaining agent on active oxygen content of cigar tobacco leaves under drought stressNote： Different small letters at the same treatment time indicate significant difference at P<0.05 level.

Fig.5 Effect of different dosages of water-retaining agent on membrane lipid peroxidation of cigar tobacco leaves under drought stressNote： Different small letters at the same treatment time indicate significant difference at P<0.05 level.

Fig.6 Effect of different dosages of water-retaining agent on the soluble protein content of cigar tobacco leaves under drought stressNote： Different small letters at the same treatment time indicate significant difference at P<0.05 level.

Table 1 Effect of different amounts of water-retaining agent on photosynthetic pigment content of cigar leaves under drought stress

处理 Treatment	叶绿素a Chlorophyll a/（mg·g^-1）	叶绿素 b Chlorophyll b/（mg·g^-1）	类胡萝卜素 Carotenoids/（mg·g^-1）	总叶绿素 Total chlorophyll/（mg·g^-1）	叶绿素 a/b Chlorophyll a/b
CK	1.12±0.08 b	0.27±0.01 b	0.31±0.02 a	1.52±0.10 b	4.09±0.18 a
T1	1.36±0.17 a	0.32±0.03 a	0.38±0.04 a	1.71±0.21 ab	4.21±0.47 a
T2	1.37±0.20 a	0.34±0.04 a	0.39±0.10 a	1.81±0.17 a	4.03±0.18 a
T3	1.51±0.01 a	0.36±0.02 a	0.37±0.02 a	1.87±0.06 a	4.28±0.26 a
T4	1.50±0.10 a	0.36±0.05 a	0.38±0.03 a	1.93±0.12 a	4.16±0.31 a

Table 2 Effect of different dosages of water-retaining agent on photosynthetic characteristics of cigar under drought stress

处理Treatment	P_n/ （μmol·m^-2·s^-1）	T_r/ （mmol·m^-2·s^-1）	G_s/ （mmol·m^-2·s^-1）	C_i/ （μL·L^-1）	WUE/ （μmol·mmol^-1）	VPD/ kPa
CK	4.20±0.10 d	1.86±0.08 c	0.05±0.02 c	274.63±10.28 a	4.73±0.16 b	5.02±0.13 a
T1	4.67±0.20 c	1.95±0.09 bc	0.08±0.01 b	253.97±0.01 b	4.91±0.05 b	4.61±0.27 b
T2	4.96±0.07 b	2.07±0.08 b	0.11±0.02 a	238.18±2.22 c	5.09±0.03 a	4.10±0.14 c
T3	5.27±0.17 a	2.26±0.09 a	0.12±0.01 a	270.93±3.72 a	5.22±0.12 a	3.83±0.16 c
T4	4.91±0.14 bc	2.01±0.12 bc	0.08±0.01 b	272.23±8.44 a	4.83±0.07 b	4.53±0.13 b

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