Journal of Agricultural Science and Technology ›› 2022, Vol. 24 ›› Issue (3): 48-56.DOI: 10.13304/j.nykjdb.2021.0599
• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles
Ning QIN1(), Junru LI1, Rui TIAN1, Zhenqi SHAO1, Xihuan LI1(
), Caiying ZHANG2(
)
Received:
2021-07-21
Accepted:
2021-09-22
Online:
2022-03-15
Published:
2022-03-14
Contact:
Xihuan LI,Caiying ZHANG
秦宁1(), 李俊茹1, 田蕊1, 邵振启1, 李喜焕1(
), 张彩英2(
)
通讯作者:
李喜焕,张彩英
作者简介:
秦宁 E-mail: 1362822834@qq.com
基金资助:
CLC Number:
Ning QIN, Junru LI, Rui TIAN, Zhenqi SHAO, Xihuan LI, Caiying ZHANG. Mining of Genetic Loci and Screening of Candidate Genes for Seed Tocopherol Content in Soybean[J]. Journal of Agricultural Science and Technology, 2022, 24(3): 48-56.
秦宁, 李俊茹, 田蕊, 邵振启, 李喜焕, 张彩英. 大豆籽粒生育酚遗传位点发掘及候选基因筛选[J]. 中国农业科技导报, 2022, 24(3): 48-56.
项目 Item | 均值 Mean | 标准差 SD | 变异系数 CV/% | 最大值 Max | 最小值 Min | 偏度 Skew | 峰度 Kurt | 显著性(P值) Sig. (P value) |
---|---|---|---|---|---|---|---|---|
α-生育酚 α-tocopherol/(μg·g‒1) | 7.10 | 2.60 | 36.62 | 15.48 | 0.91 | 0.39 | 0.15 | 2.54×10‒10 |
γ-生育酚 γ-tocopherol/(μg·g‒1) | 127.86 | 12.19 | 9.53 | 165.23 | 97.34 | 0.33 | -0.17 | 2.50×10‒10 |
δ-生育酚 δ-tocopherol/(μg·g‒1) | 65.65 | 9.21 | 14.03 | 90.58 | 39.13 | -0.06 | -0.16 | 2.34×10‒10 |
总生育酚 Total-tocopherol/(μg·g‒1) | 200.61 | 18.53 | 9.24 | 253.90 | 149.13 | 0.04 | -0.12 | 1.67×10‒10 |
α-生育酚占比 α-tocopherol proportion/% | 3.54 | 1.26 | 35.59 | 7.62 | 0.56 | 0.34 | -0.04 | 3.23×10‒10 |
γ-生育酚占比 γ-tocopherol proportion/% | 63.80 | 3.22 | 5.05 | 74.47 | 54.54 | 0.05 | 0.35 | 2.78×10‒10 |
δ-生育酚占比 δ-tocopherol proportion/% | 32.66 | 2.84 | 8.70 | 40.55 | 24.32 | -0.07 | 0.09 | 2.52×10‒10 |
Table 1 Genetic variations of seed tocopherol and its components in RIL population
项目 Item | 均值 Mean | 标准差 SD | 变异系数 CV/% | 最大值 Max | 最小值 Min | 偏度 Skew | 峰度 Kurt | 显著性(P值) Sig. (P value) |
---|---|---|---|---|---|---|---|---|
α-生育酚 α-tocopherol/(μg·g‒1) | 7.10 | 2.60 | 36.62 | 15.48 | 0.91 | 0.39 | 0.15 | 2.54×10‒10 |
γ-生育酚 γ-tocopherol/(μg·g‒1) | 127.86 | 12.19 | 9.53 | 165.23 | 97.34 | 0.33 | -0.17 | 2.50×10‒10 |
δ-生育酚 δ-tocopherol/(μg·g‒1) | 65.65 | 9.21 | 14.03 | 90.58 | 39.13 | -0.06 | -0.16 | 2.34×10‒10 |
总生育酚 Total-tocopherol/(μg·g‒1) | 200.61 | 18.53 | 9.24 | 253.90 | 149.13 | 0.04 | -0.12 | 1.67×10‒10 |
α-生育酚占比 α-tocopherol proportion/% | 3.54 | 1.26 | 35.59 | 7.62 | 0.56 | 0.34 | -0.04 | 3.23×10‒10 |
γ-生育酚占比 γ-tocopherol proportion/% | 63.80 | 3.22 | 5.05 | 74.47 | 54.54 | 0.05 | 0.35 | 2.78×10‒10 |
δ-生育酚占比 δ-tocopherol proportion/% | 32.66 | 2.84 | 8.70 | 40.55 | 24.32 | -0.07 | 0.09 | 2.52×10‒10 |
项目 Item | α-生育酚 α-tocopherol | γ-生育酚 γ-tocopherol | δ-生育酚 δ-tocopherol | 总生育酚 Total-tocopherol | α-生育酚占比 α-tocopherol proportion | γ-生育酚占比 γ-tocopherol proportion |
---|---|---|---|---|---|---|
γ-生育酚 γ-tocopherol | ‒0.018 | |||||
δ-生育酚 δ-tocopherol | 0.256** | 0.410** | ||||
总生育酚 Total-tocopherol | 0.256** | 0.859** | 0.803** | |||
α-生育酚占比 α-tocopherol proportion | 0.966** | ‒0.237** | 0.064 | 0.011 | ||
γ-生育酚占比 γ-tocopherol proportion | ‒0.515** | 0.298** | ‒0.713** | ‒0.231** | ‒0.479** | |
δ-生育酚占比 δ-tocopherol proportion | 0.154** | ‒0.233** | 0.780** | 0.256** | 0.098 | ‒0.921** |
Table 2 Correlation coefficients of tocopherol and its components in RIL population
项目 Item | α-生育酚 α-tocopherol | γ-生育酚 γ-tocopherol | δ-生育酚 δ-tocopherol | 总生育酚 Total-tocopherol | α-生育酚占比 α-tocopherol proportion | γ-生育酚占比 γ-tocopherol proportion |
---|---|---|---|---|---|---|
γ-生育酚 γ-tocopherol | ‒0.018 | |||||
δ-生育酚 δ-tocopherol | 0.256** | 0.410** | ||||
总生育酚 Total-tocopherol | 0.256** | 0.859** | 0.803** | |||
α-生育酚占比 α-tocopherol proportion | 0.966** | ‒0.237** | 0.064 | 0.011 | ||
γ-生育酚占比 γ-tocopherol proportion | ‒0.515** | 0.298** | ‒0.713** | ‒0.231** | ‒0.479** | |
δ-生育酚占比 δ-tocopherol proportion | 0.154** | ‒0.233** | 0.780** | 0.256** | 0.098 | ‒0.921** |
QTL | 单个QTL Individual QTL | 性状 Trait | 染色体Chromo-some | 左标记 Left marker | 物理位置 Physical position | 右标记 Right marker | 物理位置 Physical position | 遗传位置Genetic position | LOD | 贡献率 PVE/% | 加性效应 Additive | 区间 Confidence interval | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
左Left | 右Right | ||||||||||||
qTOC‑A2 | qα‑TOC‑A2 | α-生育酚 α-tocopherol | 8 | ss715602131 | 43 896 715 | ss715602331 | 45 913 059 | 268.4 | 5.2 | 6.26 | 0.691 | 256.4 | 269.4 |
qδ‑TOC‑A2 | δ-生育酚 δ-tocopherol | 258.2 | 3.6 | 6.77 | 2.726 | 244.6 | 269.4 | ||||||
qTotal‑TOC‑A2 | 总生育酚Total-tocopherol | 266.3 | 3.4 | 4.98 | 3.981 | 252.9 | 269.4 | ||||||
qα‑/Total‑TOC‑A2 | α-生育酚占比 α-tocopherol proportion | 267.5 | 3.3 | 4.08 | 0.269 | 253.6 | 269.4 | ||||||
qγ‑/Total‑TOC‑A2 | γ-生育酚占比 γ-tocopherol proportion | 255.1 | 2.5 | 3.85 | -0.748 | 239.3 | 269.4 | ||||||
qTOC‑A1 | qα‑TOC‑A1 | α-生育酚 α-tocopherol | 5 | ss715591799 | 40 916 521 | ss715591790 | 40 974 254 | 98.2 | 7.5 | 8.52 | 0.801 | 97.3 | 98.7 |
qγ‑/Total‑TOC‑A1 | γ-生育酚占比 γ-tocopherol proportion | 98.2 | 6.0 | 6.34 | -0.956 | 97.4 | 98.7 | ||||||
qγ‑TOC‑A1 | γ-生育酚 γ-tocopherol | ss715591790 | 40 974 254 | ss715591780 | 41 007 475 | 98.7 | 18.1 | 21.92 | -5.564 | 98.2 | 98.7 | ||
qα‑/Total‑TOC‑A1 | α-生育酚占比 α-tocopherol proportion | 98.3 | 11.9 | 13.72 | 0.491 | 98.2 | 98.7 | ||||||
qTotal‑TOC‑A1 | 总生育酚Total-tocopherol | ss715591954 | 39 886 822 | ss715591831 | 40 684 517 | 94.3 | 9.1 | 12.32 | -6.226 | 90.4 | 96.4 | ||
qTOC‑H | qα‑TOC‑H | α-生育酚 α-tocopherol | 12 | ss715611499 | 1 379 873 | ss715613700 | 980 800 | 153.7 | 5.5 | 6.22 | -0.685 | 147.8 | 153.7 |
qα‑/Total‑TOC‑H | α-生育酚占比 α-tocopherol proportion | 152.9 | 5.1 | 5.83 | -0.320 | 147.5 | 157.9 | ||||||
qγ‑/Total‑TOC‑H | γ-生育酚占比 γ-tocopherol proportion | 153.8 | 3.9 | 4.10 | 0.769 | 150.0 | 156.7 | ||||||
qTOC‑F | qγ‑TOC‑F | γ-生育酚 γ-tocopherol | 13 | ss715616114 | 39 590 255 | ss715616142 | 39 911 642 | 116.6 | 6.6 | 7.53 | -3.272 | 115.7 | 119.2 |
qTotal‑TOC‑F | 总生育酚Total-tocopherol | 116.5 | 5.0 | 6.26 | -4.454 | 115.7 | 119.4 | ||||||
qTOC‑G | qδ‑TOC‑G | δ-生育酚 δ-tocopherol | 18 | ss715629792 | 20 419 500 | ss715629811 | 20 502 705 | 104.5 | 3.8 | 4.50 | 2.222 | 104.4 | 104.8 |
qTotal‑TOC‑G | 总生育酚Total-tocopherol | ss715629923 | 21 629 092 | ss715629930 | 21 908 634 | 106.4 | 2.7 | 3.21 | 3.191 | 105.5 | 106.8 | ||
qδ‑/Total‑TOC‑G | δ-生育酚占比 δ-tocopherol proportion | ss715629675 | 19 772 410 | ss715629709 | 19 955 096 | 103.6 | 3.4 | 6.19 | 0.643 | 103.3 | 103.6 | ||
qTOC‑L | qα‑TOC‑L | α-生育酚 α-tocopherol | 19 | ss715633071 | 514 053 | ss715636028 | 580 135 | 116.0 | 4.9 | 5.43 | 0.639 | 115.8 | 116.8 |
qα‑/Total‑TOC‑L | α-生育酚占比 α-tocopherol proportion | ss715636293 | 809 326 | ss715636173 | 674 202 | 110.1 | 5.2 | 5.77 | 0.318 | 104.0 | 113.4 | ||
qTOC‑M | qγ‑TOC‑M | γ-生育酚 γ-tocopherol | 7 | ss715598313 | 5 265 136 | ss715598195 | 4 429 189 | 99.2 | 3.5 | 4.43 | 2.503 | 92.2 | 107.6 |
qTotal‑TOC‑M | 总生育酚Total-tocopherol | ss715597765 | 3 872 947 | ss715597135 | 3 167 571 | 110.1 | 2.6 | 3.08 | 3.112 | 109.2 | 115.8 |
Table 3 Pleiotropic QTLs of seed tocopherol and its components in soybean RIL population
QTL | 单个QTL Individual QTL | 性状 Trait | 染色体Chromo-some | 左标记 Left marker | 物理位置 Physical position | 右标记 Right marker | 物理位置 Physical position | 遗传位置Genetic position | LOD | 贡献率 PVE/% | 加性效应 Additive | 区间 Confidence interval | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
左Left | 右Right | ||||||||||||
qTOC‑A2 | qα‑TOC‑A2 | α-生育酚 α-tocopherol | 8 | ss715602131 | 43 896 715 | ss715602331 | 45 913 059 | 268.4 | 5.2 | 6.26 | 0.691 | 256.4 | 269.4 |
qδ‑TOC‑A2 | δ-生育酚 δ-tocopherol | 258.2 | 3.6 | 6.77 | 2.726 | 244.6 | 269.4 | ||||||
qTotal‑TOC‑A2 | 总生育酚Total-tocopherol | 266.3 | 3.4 | 4.98 | 3.981 | 252.9 | 269.4 | ||||||
qα‑/Total‑TOC‑A2 | α-生育酚占比 α-tocopherol proportion | 267.5 | 3.3 | 4.08 | 0.269 | 253.6 | 269.4 | ||||||
qγ‑/Total‑TOC‑A2 | γ-生育酚占比 γ-tocopherol proportion | 255.1 | 2.5 | 3.85 | -0.748 | 239.3 | 269.4 | ||||||
qTOC‑A1 | qα‑TOC‑A1 | α-生育酚 α-tocopherol | 5 | ss715591799 | 40 916 521 | ss715591790 | 40 974 254 | 98.2 | 7.5 | 8.52 | 0.801 | 97.3 | 98.7 |
qγ‑/Total‑TOC‑A1 | γ-生育酚占比 γ-tocopherol proportion | 98.2 | 6.0 | 6.34 | -0.956 | 97.4 | 98.7 | ||||||
qγ‑TOC‑A1 | γ-生育酚 γ-tocopherol | ss715591790 | 40 974 254 | ss715591780 | 41 007 475 | 98.7 | 18.1 | 21.92 | -5.564 | 98.2 | 98.7 | ||
qα‑/Total‑TOC‑A1 | α-生育酚占比 α-tocopherol proportion | 98.3 | 11.9 | 13.72 | 0.491 | 98.2 | 98.7 | ||||||
qTotal‑TOC‑A1 | 总生育酚Total-tocopherol | ss715591954 | 39 886 822 | ss715591831 | 40 684 517 | 94.3 | 9.1 | 12.32 | -6.226 | 90.4 | 96.4 | ||
qTOC‑H | qα‑TOC‑H | α-生育酚 α-tocopherol | 12 | ss715611499 | 1 379 873 | ss715613700 | 980 800 | 153.7 | 5.5 | 6.22 | -0.685 | 147.8 | 153.7 |
qα‑/Total‑TOC‑H | α-生育酚占比 α-tocopherol proportion | 152.9 | 5.1 | 5.83 | -0.320 | 147.5 | 157.9 | ||||||
qγ‑/Total‑TOC‑H | γ-生育酚占比 γ-tocopherol proportion | 153.8 | 3.9 | 4.10 | 0.769 | 150.0 | 156.7 | ||||||
qTOC‑F | qγ‑TOC‑F | γ-生育酚 γ-tocopherol | 13 | ss715616114 | 39 590 255 | ss715616142 | 39 911 642 | 116.6 | 6.6 | 7.53 | -3.272 | 115.7 | 119.2 |
qTotal‑TOC‑F | 总生育酚Total-tocopherol | 116.5 | 5.0 | 6.26 | -4.454 | 115.7 | 119.4 | ||||||
qTOC‑G | qδ‑TOC‑G | δ-生育酚 δ-tocopherol | 18 | ss715629792 | 20 419 500 | ss715629811 | 20 502 705 | 104.5 | 3.8 | 4.50 | 2.222 | 104.4 | 104.8 |
qTotal‑TOC‑G | 总生育酚Total-tocopherol | ss715629923 | 21 629 092 | ss715629930 | 21 908 634 | 106.4 | 2.7 | 3.21 | 3.191 | 105.5 | 106.8 | ||
qδ‑/Total‑TOC‑G | δ-生育酚占比 δ-tocopherol proportion | ss715629675 | 19 772 410 | ss715629709 | 19 955 096 | 103.6 | 3.4 | 6.19 | 0.643 | 103.3 | 103.6 | ||
qTOC‑L | qα‑TOC‑L | α-生育酚 α-tocopherol | 19 | ss715633071 | 514 053 | ss715636028 | 580 135 | 116.0 | 4.9 | 5.43 | 0.639 | 115.8 | 116.8 |
qα‑/Total‑TOC‑L | α-生育酚占比 α-tocopherol proportion | ss715636293 | 809 326 | ss715636173 | 674 202 | 110.1 | 5.2 | 5.77 | 0.318 | 104.0 | 113.4 | ||
qTOC‑M | qγ‑TOC‑M | γ-生育酚 γ-tocopherol | 7 | ss715598313 | 5 265 136 | ss715598195 | 4 429 189 | 99.2 | 3.5 | 4.43 | 2.503 | 92.2 | 107.6 |
qTotal‑TOC‑M | 总生育酚Total-tocopherol | ss715597765 | 3 872 947 | ss715597135 | 3 167 571 | 110.1 | 2.6 | 3.08 | 3.112 | 109.2 | 115.8 |
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