Optimization on Extraction of Polysaccharide from Honeysuckle （Lonicera japonica） Leaves and Its Antibacterial Activity

doi:10.13304/j.nykjdb.2023.0312

Abstract

Abstract:

In order to clarify the antibacterial and moisturizing effects of polysaccharide from honeysuckle leaves in vitro， the polysaccharide was extracted by the optimized water extraction and alcohol precipitation method in this experiment. The antibacterial experiments against Escherichia coli， Bacillus subtilis and Staphylococcusaureus were carried out by the pouring plate with pre-additive bacterial solution and punching method. The polysaccharide from Lonicera japonica leaves was used to prepare the polysaccharide moisturizing cream so as to explore its moisturizing effect. The results showed that the content of polysaccharide was （17.29%±0.41%） using the optimized extraction method. The polysaccharide of Lonicera japonica leaves had inhibitory effects on the 3 bacteria， among them， the inhibitory effect on Escherichia coli was the best， with an average diameter of （15.80±0.58） mm， which was highly sensitive. The skin moisture test showed that the polysaccharide from Lonicera japonica leaves had good skin rehydration and moisturizing properties， which had potential development value. The extraction process of polysaccharides from Lonicera japonica leaves， laying a foundation for the deep development of Lonicera japonica and continuously improving its added value.

Key words: leaves of Lonicera japonica, polysaccharide, antibacterial test, moisturizing

摘要：

为明确忍冬叶多糖体外抑菌及保湿效果，以优化后的水提醇沉法提取忍冬叶多糖，采用预加菌液倾注平板－打孔法，验证忍冬叶多糖对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌的抑菌作用；并利用忍冬叶多糖制备忍冬多糖保湿霜，探究忍冬叶多糖的皮肤保湿效果。结果表明，优化后的提取方法所得的多糖含量为（17.29%±0.41%）。忍冬叶多糖对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌均有抑制作用，对大肠杆菌的抑制效果最佳，平均抑菌圈直径为（15.80±0.58） mm。皮肤水分测试结果表明，忍冬叶多糖具有良好的皮肤补水性及保湿性，具有潜在开发价值。以上研究结果优化了忍冬叶多糖提取工艺，为忍冬的深度开发及提高忍冬附加值奠定了基础。

关键词: 忍冬叶, 多糖, 抑菌试验, 保湿

CLC Number:

S377

Ruiwu SHI, Sufang ZHANG, Lina NIU, Tian SU, Xiaoqian ZHAO, Dongchen NA, Wei CHEN. Optimization on Extraction of Polysaccharide from Honeysuckle （Lonicera japonica） Leaves and Its Antibacterial Activity[J]. Journal of Agricultural Science and Technology, 2023, 25(11): 218-226.

史瑞武, 张素芳, 牛丽娜, 苏田, 赵晓茜, 那冬晨, 陈伟. 忍冬叶多糖提取工艺优化及抗菌活性分析[J]. 中国农业科技导报, 2023, 25(11): 218-226.

Figures/Tables 6

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成分Component	含量Content	成分 Component	含量Content
乳木果油 Shea butter	3 g	山梨酸钾 Potassium sorbate	0.12 g
蜂蜡Bee wax	1.2 g	甜杏仁油Sweet almond	0.3 mL
鲸蜡硬脂醇 Cetearyl alcohol	7 g	橄榄油Olive oil	0.3 mL
卡波姆Homopolymer	0.6 g	氢化聚葵烯Hydrogenated polysuncene	1 mL
玻尿酸Hyaluronic acid	0.3 g	GTCC	1 mL
柠檬酸Citric acid	0.1 g	甘油Glycerol	2 mL
EDTA-Na₂	0.06 g	神经酰胺Ceramide	8 mL
无水甜菜碱Betaine Anhydrous	3 g	丁二醇1，2-Butanediol	2 mL
海藻糖Trehalose	2.4 g	忍冬叶多糖 Polysaccharide of Lonicera japonica leaves	0.5/1.0 g

成分Component	含量Content	成分 Component	含量Content
乳木果油 Shea butter	3 g	山梨酸钾 Potassium sorbate	0.12 g
蜂蜡Bee wax	1.2 g	甜杏仁油Sweet almond	0.3 mL
鲸蜡硬脂醇 Cetearyl alcohol	7 g	橄榄油Olive oil	0.3 mL
卡波姆Homopolymer	0.6 g	氢化聚葵烯Hydrogenated polysuncene	1 mL
玻尿酸Hyaluronic acid	0.3 g	GTCC	1 mL
柠檬酸Citric acid	0.1 g	甘油Glycerol	2 mL
EDTA-Na₂	0.06 g	神经酰胺Ceramide	8 mL
无水甜菜碱Betaine Anhydrous	3 g	丁二醇1，2-Butanediol	2 mL
海藻糖Trehalose	2.4 g	忍冬叶多糖 Polysaccharide of Lonicera japonica leaves	0.5/1.0 g

菌种 Bacterial species	对照 CK3	试验组 Experimental group
菌种 Bacterial species	对照 CK3	M1	M2	M3	平均值 Mean value
大肠杆菌 Escherichia coli	0.00	16.50	15.50	15.50	15.80±0.58
枯草芽孢杆菌 Bacillus subtilis	0.00	11.50	12.00	14.50	12.70±1.61
金黄色葡萄球菌 Staphylococcus aureus	0.00	13.50	14.00	12.54	13.30±0.74

菌种 Bacterial species	对照 CK3	试验组 Experimental group
菌种 Bacterial species	对照 CK3	M1	M2	M3	平均值 Mean value
大肠杆菌 Escherichia coli	0.00	16.50	15.50	15.50	15.80±0.58
枯草芽孢杆菌 Bacillus subtilis	0.00	11.50	12.00	14.50	12.70±1.61
金黄色葡萄球菌 Staphylococcus aureus	0.00	13.50	14.00	12.54	13.30±0.74

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