Application of UV-visible Spectral Library in Recessive Screening of Pesticide Fungicides

doi:10.13304/j.nykjdb.2023.0247

Abstract

Abstract:

To establish the ultraviolet-visible （UV-VIS） spectral library to quickly and accurately retrieve the hidden components added in the pesticide fungicide， with acetonitrile and water as the mobile phase， the UV-VIS spectrograms of 98 conventional bactericide standards were separated and extracted by gradient elution using a high-performance liquid chromatograph， and the UV-VIS spectrograms of the standard substances were used to establish the UV-VIS spectrogram library. The target compound was matched according to the spectral similarity and the retention time of the standard sample， so as to retrieve the hidden components added in the pesticide preparation. 2 bactericides were used to verify the accuracy of the spectral library retrieval， and the results showed that the accuracy of the spectral library retrieval reached 100%. This method had the advantages of high accuracy， easy operation and low cost.

Key words: high-performance liquid chromatography, bactericide, ultraviolet-visible spectrum library, pesticide

摘要：

为建立紫外-可见光谱库以期能够快速准确的检索农药杀菌剂中添加的隐性成分，通过高效液相色谱仪，以乙腈和水做为流动相，采用梯度洗脱分离及提取98种常规杀菌剂标准物质的紫外-可见光谱图，以标准物质的紫外-可见光谱图来建立紫外-可见光谱库。根据光谱的相似度和标样的保留时间来匹配目标化合物，从而检索出农药制剂中添加的隐性成分。通过2个杀菌剂来验证谱库检索的准确性，结果显示，谱库检索的正确性达100%。该方法具有检测目标化合物准确率高、操作简单、易学、成本较低等优点。

关键词: 高效液相色谱仪, 杀菌剂, 紫外-可见光谱库, 农药

CLC Number:

Ying CHEN, Xiaofeng LIU, Yiping CHEN, Liping LIAO, Hongtao FU. Application of UV-visible Spectral Library in Recessive Screening of Pesticide Fungicides[J]. Journal of Agricultural Science and Technology, 2024, 26(12): 122-128.

陈颖, 刘小锋, 陈一萍, 廖丽萍, 傅洪涛. 紫外-可见光谱库在农药杀菌剂隐性排查中的应用[J]. 中国农业科技导报, 2024, 26(12): 122-128.

Figures/Tables 15

References 21

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时间 Time/min	单元 Unit	处理命令 Processing command	值 Value
0.01	泵 Pump	BConc	30
8.00	泵 Pump	BConc	30
8.01	泵 Pump	BConc	72
18.00	泵 Pump	BConc	72
18.01	泵 Pump	BConc	92
27.00	泵 Pump	BConc	92
27.01	泵 Pump	BConc	30
34.00	泵 Pump	BConc	30
34.01	控制器 Controller	停止 Stop	—

时间 Time/min	单元 Unit	处理命令 Processing command	值 Value
0.01	泵 Pump	BConc	30
8.00	泵 Pump	BConc	30
8.01	泵 Pump	BConc	72
18.00	泵 Pump	BConc	72
18.01	泵 Pump	BConc	92
27.00	泵 Pump	BConc	92
27.01	泵 Pump	BConc	30
34.00	泵 Pump	BConc	30
34.01	控制器 Controller	停止 Stop	—

峰 Peak	A1峰 Peak A1
序列号 Serial number	13
相似度 Similarity	0.999 971
化合物名称 Name of compound	嘧菌酯标 Azoxystrobin
保留时间 Retention time/min	16.237

峰 Peak	A1峰 Peak A1
序列号 Serial number	13
相似度 Similarity	0.999 971
化合物名称 Name of compound	嘧菌酯标 Azoxystrobin
保留时间 Retention time/min	16.237

峰 Peak	A2峰 Peak A2
序列号 Serial number	585
相似度 Similarity	0.999 917
化合物名称 Name of compound	苯醚甲环唑标 Difenoconazole
保留时间 Retention time/min	19.584