Journal of Agricultural Science and Technology ›› 2022, Vol. 24 ›› Issue (12): 142-152.DOI: 10.13304/j.nykjdb.2022.0995
• INNOVATIVE GERMPLASM • Previous Articles Next Articles
Peng ZHU1(), Ke XU1, Xitie LING2, Jinyan WANG2, Yuwen YANG2, Baolong ZHANG2, Shi QIU2(
)
Received:
2022-11-17
Accepted:
2022-12-05
Online:
2022-12-15
Published:
2023-02-06
Contact:
Shi QIU
朱鹏1(), 许轲1, 凌溪铁2, 王金彦2, 杨郁文2, 张保龙2, 裘实2(
)
通讯作者:
裘实
作者简介:
朱鹏 E-mail:1203759422@qq.com;
基金资助:
CLC Number:
Peng ZHU, Ke XU, Xitie LING, Jinyan WANG, Yuwen YANG, Baolong ZHANG, Shi QIU. Excavation and Utilization of Herbicide-resistant Rice and Wheat Germplasm Resources Under Rice-Wheat Rotation System[J]. Journal of Agricultural Science and Technology, 2022, 24(12): 142-152.
朱鹏, 许轲, 凌溪铁, 王金彦, 杨郁文, 张保龙, 裘实. 稻麦轮作体系下抗除草剂稻麦种质资源的挖掘与利用[J]. 中国农业科技导报, 2022, 24(12): 142-152.
1 | XU L, LI X X, WANG X Y, et al.. Comparing the grain yields of direct-seeded and transplanted rice: a meta-analysis [J/OL]. Agronomy, 2019,9(11):767[2022-10-16]. . |
2 | 李儒海. 稻麦(油)两熟田杂草子实的水流传播机制及杂草可持续管理模式的研究[D]. 南京:南京农业大学,2009. |
LI R H. Mechanisms on weed seed dispersal by irrigation water and sustainable weed management strategy under rice-wheat/oilseed rape double cropping system [D]. Nanjing:Nanjing Agricultural University, 2009. | |
3 | 张为农. 我国水稻除草剂发展趋势[J]. 农药市场信息,2014(6):33-34. |
ZHANG W N. Development trend of rice herbicide in China [J]. Pesticide Market News, 2014(6):33-34. | |
4 | 赵平, 严秋旭, 李新, 等. 全球水稻田除草剂的使用现状及市场[J]. 农药,2012,51(11):781-784. |
ZHAO P, YAN Q X, LI X, et al.. Global market and use of herbicides in rice field [J]. Agrochemicals,2012,51(11): 781-784. | |
5 | HAKIM A R, JURAIMI S M, REZAUL K, et al.. Effectiveness of herbicides to control rice weeds in diverse saline environments [J/OL]. Sustainability, 2021,13(4): 2053[2022-10-16]. . |
6 | RAO A N, JOHNSON D E, SIYAPRASAD B, et al.. Weed management in direct-seeded rice [J]. Adv. Agron.,2007,93(6):153-255. |
7 | CHAUHAN B S. Weed ecology and weed management strategies for dry-seeded rice in Asia [J/OL]. Weed Technol., 2017, 26(1):1-13 [2022-10-16]. . |
8 | CHAUHAN B, GILL G. Ecologically based weed management strategies [M]// CHAUHAN B, MAHAJAN G. Recent Advances in Weed Management. New York: Springer, 2014. |
9 | HOLZNER W. Concepts categories and characteristics of weeds [M/OL]// HOLZNER W, NUMATA M. Biology and Ecology of Weeds. Dordrecht: Springer, . |
10 | 张一宾, 张怿, 伍贤英. 世界农药新进展(三)[M]. 北京:化学工业出版社,2013:74-75. |
11 | 王险峰, 关成宏, 辛明远. 我国长残效除草剂使用概况、问题及对策[J]. 农药,2003,42(11):7-12. |
WANG X F, GUAN C H, XIN M Y. Long residual herbicides in China-current status problems and solutions [J]. Agrochenicals, 2003,42(11):7-12. | |
12 | 张朝贤,黄红娟,崔海兰,等. 抗药性杂草与治理[J]. 植物保护,2013,39(5):99-102. |
ZHANG C X, HUANG H J, CUI H L, et al.. Herbicide-resistant weeds and their management [J]. Plant Prot., 2013,39(5):99-102. | |
13 | 谷涛, 李永丰, 张自常,等. 杂草对激素类除草剂抗药性研究进展[J]. 植物保护,2021,47(1):15-26. |
GU T, LI Y F, ZHANG Z C, et al.. Research progress in weed resistance to auxin herbicides [J]. Plant Prot., 2021,47(1):15-26. | |
14 | 梁雪莲,王引斌,卫建强,等. 作物抗除草剂转基因研究进展[J]. 生物技术通报,2001(2):17-21. |
LIANG X L, WANG Y B, WEI J Q, et al.. The progress of studies on herbicide resistance gene of crop [J]. Biotechnol. Information, 2001(2):17-21. | |
15 | BECKIE H J, HARKER K N, HALL L M, et al.. A decade of herbicide-resistant crops in Canada [J]. Can. J. Plant Sci.,2006,86(4):1243-1264. |
16 | CONRAD A, PIEPER S, HOPPE H W, et al.. Glyphosate in German adults-time trend (2001 to 2015) of human exposure to a widely used herbicide [J]. Int. J. Hyg. Environ. Health, 2017,220(1):8-16. |
17 | DUKE S O, POWLES S B. Glyphosate: a once-in-a-century herbicide [J]. Pest Manag. Sci., 2008, 64(4):319-325. |
18 | SMART C C, MULLERG G, AMRHEIN N, et al.. Selective overproduction of 5-enol-pyruvylshikimic acid 3-phosphate synthase in a plant cell culture which tolerates high doses of the herbicide glyphosate [J]. J. Biol. Chem.,1985,260(30):16338-16346. |
19 | SIMRNJEET S, VIJAY K, JATINDER P K G, et al.. Herbicide glyphosate: toxicity and microbial degradation [J/OL]. Int. J. Environ. Res. Public Health, 2020, 17(20):7519[2022-10-16].. |
20 | STEINRUCKEN H C, AMRHEIN N. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase [J]. Biochem. Biophys. Res. Commun., 1980,94(4):1207-1212. |
21 | BOGER P, SANDYMAN G. Modern herbicides affecting typical plant processes [C]// HAUG G, HOFFMANN H. Controlled Release, Biochemical Effects of Pesticides, Inhibition of Plant Pathogenic Fungi. Chemistry of Plant Protection. Berlin, Heidelberg: Springer, 1990. |
22 | LEA P J. The inhibition of ammonia assimilation: a mechanism of herbicide action [J]. Topics Photosynth., 1991,10(6):267-298. |
23 | GONZALEZ-MORO M B, LOUREIRO-BELDARRIAN I, ESTAVILLO J M, et al.. Effect of photorespiratory C2 acids on CO2 assimilation, PS II photochemistry and the xanthophyll cycle in maize [J]. Photosynth. Res., 2003,78(2):161-73. |
24 | CHIPMAN D, BARAK Z, SCHLOSS J V. Biosynthesis of 2-aceto-2-hydroxy acids: acetolactate synthases and acetohydroxyacid synthase [J]. Biochim. Biophys. Acta, 1998,1385(2):401-419. |
25 | GRANDONI J A, MARTA P T, SCHLOSS J V. Inhibitors of branched-chain amino acid biosynthesis as potential antituberculosis agents [J]. Antimicrob. Chemoth., 1998,42(4):475-482. |
26 | SHOBA D, RAVEENDRAN M, MANONMANI S, et al.. Development and genetic characterization of a novel herbicide (Imazethapyr) tolerant mutant in rice (Oryza sativa L.) [J/OL]. Rice, 2017,10(1):10[2022-10-16]. . |
27 | GARCIA M D, NOUWENS A, LONHIENNE T G, et al.. Comprehensive understanding of acetohydroxyacid synthase inhibition by different herbicide families [J]. Proc. Nat. Acad. Sci. USA, 2017, 114(7):1091-1100. |
28 | LIU Y D, LL Y Y, WANG X Y. Acetohydroxyacid synthases: evolution, structure, and function [J]. Appl. Microbiol. Biotechnol., 2016,100(20):8633-8649. |
29 | DEYBACH J C, DASILVA V, GRANDCHAMP B, et al.. The mitochondrial location of protoporphyrinogen oxidase [J]. Eur. Biochem., 1985,149(2):431-435. |
30 | MATRINGE M, SCALLA R. Studies on the mode of action of acifluorfen-methyl in nonchlorophyllous cells: accumulation of tetrapyrroles [J]. Plant Physiol., 1988,86(2):619-622. |
31 | SALAS R A, BURGOS N R, TRANEL P J, et al.. Resistance to PPO-inhibiting herbicide in palmer amaranth from Arkansas [J]. Pest Manag. Sci., 2016,72(5):864-869. |
32 | SALAS-PEREZ R A, BURGOS N R, RANGANI G, et al.. Frequency of Gly-210 deletion mutation among protoporphyrinogen oxidase inhibitor-resistant palmer amaranth (Amaranthus palmeri) populations [J]. Weed Sci., 2017,65 (6):718-731. |
33 | BEAUDENIES R, EDMUANDS A J F, FRASER T E M, et al.. Herbicidal 4-hydroxyphenylpyruvate dioxygenase inhibitors-a review of the triketone chemistry story from a syngenta perspective [J]. Bioorg. Med. Chem., 2009,17(12):4134-4152. |
34 | FOYER C H, DESCOURVIERES P, KUNERT K J. Protection against oxygen radicals: an important defence mechanism studied in transgenic plants [J]. Plant Cell Environ.,1997,17(5):507-523. |
35 | NORRIS S R, BARRETTE T R, DELLA P D. Genetic dissection of carotenoid synthesis in Arabidopsis defines plastoquinone as an essential component of phytoene desaturation [J]. Plant Cell, 1995,7(12):2139-2149. |
36 | HESS F D. Light-dependent herbicides: an overview [J]. Weed Sci.,2000,48(2):160-170. |
37 | AHRENS H, LANGE G, MULLER T, et al.. 4-hydroxyphenylpyruvate dioxygenase inhibitors in combination with safeners: solutions for modern and sustainable agriculture [J]. Angew. Chem. Int. Edit., 2013,52(36): 9388-9398. |
38 | KAUNDUN S S. Resistance to acetyl-CoA carboxylaseinhibiting herbicides [J]. Pest Manag. Sci., 2014,70(9):1405-1417. |
39 | BUSI R, GOGGIN D E, HEAP I M, et al.. Weed resistance to synthetic auxin herbicides [J]. Pest Manage. Sci., 2018,74(10):2265-2276. |
40 | ALICE A W, VIJAY K N, LOGAN G, et al.. Characterization of fenoxaprop-P-ethyl-resistant Junglerice (Echinochloa colona) from Mississippi [J]. Weed Sci., 2016,64(4):588-595. |
41 | BASAK S, ALAM M J, GOODWIN D, et al.. Detecting the effect of ACCase-targeting herbicides on ACCase activity utilizing a malachite green colorimetric functional assay [J]. Weed Sci., 2022,70(1):14-19. |
42 | KE J, WEN T N, NIKOLAU B J, et al.. Coordinate regulation of the nuclear and plastidic genes coding for subunits of the heteromeric acetyl-coenzyme a carboxylase [J]. Plant Physiol., 2000,122(4):1057-1071. |
43 | NG C H, RATNAM W, SURIF S, et al.. Inheritance of glyphosate resistance in goosegrass (Eleusine indica) [J]. Weed Sci., 2004,52(4):564-570. |
44 | ZHU B, HUANG D N, YANG W. Production of herbicide-resistant transgenic rice plants from immature embryos using biolistic method [J]. Sci. Agric. Sin., 1996,29(6):15-20. |
45 | GROSSMANN K. Auxin herbicides:current status of mechanism and mode of action [J]. Pest Manage. Sci.,2010,66(2):113-120. |
46 | ROSS J J, O’NEILL D P, WOLBANG C M, et al.. Auxin-gibberellin interactions and their role in plant growth [J]. Plant Growth Regul., 2001,20(4):336-353. |
47 | KOU J P, LI N, SONG G W, et al.. Knowledge about Agricultural GMOs-100 Questions. [M] 2nd. edn. Beijing: China Agriculture Press, 2012. |
48 | 蒋田田, 文君慧. 我国抗除草剂转基因作物面临的机遇和挑战[J]. 安徽农业科学,2021,49(22):239-242. |
JIANG T T, WEN J H. Opportunities and challenges facing genetically modified herbicide-resistant crops in China [J]. Anhui. Agric. Sci., 2021,49(22):239-242. | |
49 | 张玉池, 王晓蕾, 徐文蓉, 等. 国内外抗除草剂基因专利的分析[J]. 杂草学报,2017,35(2):1-22. |
ZHANG Y C, WANG X L, XU W R, et al.. Analysis on the patents of herbicide resistance gene at home and abroad [J]. J. Weed Sci., 2017,35(2):1-22. | |
50 | 童汉华, 曹一平, 章善庆. 耐草甘膦作物的选育研究概况[J]. 中国稻米,2006,12(6):12-14. |
TONG H H, CAO Y P, ZHANG S Q. Overview of research on breeding of glyphosate-tolerant crops [J]. China Rice, 2006,12(6):12-14. | |
51 | CHHAPEKAR S, RAGHAVENDRARAO S, PAVAN G, et al.. Transgenic rice expressing a codon-modified synthetic CP4- EPSPS confers tolerance to broad-spectrum herbicide, glyphosate [J]. Plant Cell Rep., 2015,34(5):721-731. |
52 | 王云鹏, 马景勇, 马瑞, 等. 土壤宏基因组中抗草甘膦新基因的克隆与转化水稻的研究[J]. 作物学报,2014,40(7):1190- 1196. |
WANG Y P, MA J Y, MA R, et al.. Cloning of new herbicide resistant gene in soil metagenomics and generation of transgenic rice plants [J]. Acta Agron. Sin.,2014,40(7):1190-1196. | |
53 | 梅锋. 转基因抗虫耐草甘膦水稻在浙江省的生产性试验[D]. 杭州:浙江大学,2015. |
MEI F. Production test of transgenic insect-resistant glyphosate tolerant rice in Zhejiang province [D]. Hangzhou: Zhejiang University, 2015 | |
54 | 董玉凤. 转G2-aroA基因抗草甘膦水稻的获得及G2-EPSPS蛋白拆分重组后的草甘膦抗性分析[D]. 北京:中国农业科学院,2017. |
DONG Y F. Glyphosate resistance of transgenic rice with G2-aroA gene and glyphosate resistance analysis after G2-EPSPS protein resolution and recombination [D]. Beijing: Chinese Academy of Agricultural Sciences,2017. | |
55 | 陈海伟, 张鲁华, 陈德富, 等. 除草剂及抗除草剂作物的应用现状与展望[J]. 生物技术通报,2012(10):35-40. |
CHEN H W, ZHANG L H, CHEN D F, et al.. Current utilization status and future prospect of herbicide and herbicide-resistant crops [J]. Biotechnol. Inform., 2012(10):35-40. | |
56 | DUKE S O, POWLES S B. Glyphosate-resistant crops and weeds:now and in the future [J]. Agbioforum,2009, 12(3-4):346-357. |
57 | XIAO G Y, YUAN L P, SUN S S M. Strategy and utilization of a herbicide resistance gene in two-line hybrid rice [J]. Mol. Breed.,2007,20(2):287-292. |
58 | WANG S, SHI L, SUN Z, et al.. Agrobacterium-mediated transformation of rice (Oryza sativa L.) with atzA gene [J]. Agric. Sci. China, 2004,37(8):1093-1098. |
59 | 黄大年, 李敬阳, 章善庆. 用除草剂基因快速检测和提高杂交稻纯度的新技术[J]. 科学通报,1998,44(1):67-70. |
HUANG D N, LI J Y, ZHANG S Q. A new technique for rapid detection of herbicide genes and improving the purity of hybrid rice [J]. Sci. Bull., 1998,44(1):67-70. | |
60 | 秦冠男, 翁绿水, 肖国樱. 水稻密码子优化基因Mat# 增强草铵膦抗性[J]. 农业生物技术学报,2016,24(10):1457-1465. |
QIN G N, WENG LS, XIAO G Y. The Mat# gene optimized by codon bias of rice (Oryza sativa) enhances resistance to glufosinate [J]. J. Agric. Biotechnol., 2016, 24(10): 1457-1465. | |
61 | LI Z, HAYASHIMOTO A, MURAI N A. Sulfonylurea herbicide-resistance gene from Arabiopsis thaliana as a new selectiable market for production of fertile transgenic rice plants [J]. Plant Physiol., 1992,100(2):662-668. |
62 | KAWAI K, KAKU K, IZAWA N, et al.. Functional analysis of transgenic rice plants expressing a novel mutated ALS gene of rice [J]. J. Pesticide Sci., 2007,32(4): 385- 392. |
63 | LEE H J, LEE S B, CHUNG J S, et al.. Transgenic rice plants expressing a bacillus subtilis protoporphyrinogen oxidase gene are resistance to diphenyl ether herbicide oxyfluorfen [J]. Plant Cell Physiol., 2000,41(6):743-749. |
64 | JUNG H, KUK I Y, BACK K, et al.. Resistance pattern and antioxidant enzyme profiles of protoporphyrinogen oxidase(PROTOX) inhibitor-resistant transgenic rice [J]. Pesticide Biochem. Physiol., 2008,91(1):53-65. |
65 | LIANG Y, MINAMI H, SATO F. Isolation of herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase from cultured coptis japonica cells [J]. Biosci. Biotechnol. Biochem., 2008,72(11):3058- 3062. |
66 | LEE C M, YEO Y S, LEE J H, et al.. Identification of a novel 4-hydroxyphenylpyruvate dioxygenase from the soil metagenome [J]. Biochem. Biophys. Res. Commun., 2008,370(2):322-326. |
67 | 黄彦,夏冰洁,崔中利. 硝磺草酮抗性菌株的筛选及抗性基因的克隆表达[J]. 微生物学通报, 2015,42(10);1895-1902. |
HUANG Y, XIA B J, CUI Z L. Isolation of mesotrione-resistant strain and cloning and expression of HPPD [J]. Microbiol. China, 2015, 42(10):1895-1902. | |
68 | 高爱保, 郭平毅. 谷子抗除草剂的质体ACCase基因及系统发育分析[J]. 中国农学通报,2016,32(30):61-67. |
GAO A B, GUO P Y. Herbicide resistance ACCase gene in setaria italic and molecular phylogeny [J]. Chin. Agric. Sci. Bull., 2016,32(30):61-67. | |
69 | BEHRENS M R, MUTLU N, CHAKRABORTY S, et al.. Dicamba resistance: enlarging and preserving biotechnology-based weed management strategies [J]. Science,2007,316(5828):1185-1188. |
70 | 苏少泉. 非转基因抗耐除草剂作物的开发与应用[J]. 世界农药,2013,10(5):27-29. |
SU S Q. The development and use of non-transgenic (tolerance) crops [J]. World Pesticides, 2013,10(5):27-29. | |
71 | 魏松红,纪明山,王英姿, 等. 应用诱变法筛选抗草甘膦小麦植标初步研究[J]. 现代农药,2006,6(3):42-44. |
WEI S H, JI M S, WANG Y Z, et al. Selection of glyphosate-resistant wheat with mutation [J]. Mod. Agrochem., 2006,6(3):42-44. | |
72 | LI J, MENG X B, ZONG Y, et al.. Gene replacements and insertions in rice by intron targeting using CRISPR-Cas9 [J/OL]. Nat. Plants, 2016(2):16139[2022-10-16]. . |
73 | ENDO M, OSAKABE K, ONO K, et al.. Molecular breeding of a novel herbicide-tolerant rice by gene targeting [J]. Plant J., 2007,52(1):157-166. |
74 | 唐晓艳, 柳威, 王承旭, 等. 水稻抗除草剂蛋白及其在植物育种中的应用的制作方法[P]. 中国:CN102586215A, 2012-07-18. |
75 | 夏兰琴, 孙永伟, 赵云德, 等. 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用的制作方法[P]. 中国:CN106811479A,2017-06-09. |
76 | 张保龙, 陈天子, 凌溪铁, 等. EMS诱变水稻创制抗咪唑啉酮除草剂种质[J]. 核农学报,2021,35(2):253-261. |
ZHANG B L, CHEN T Z, LING X T, et al.. Screening of imidazolinone-resistant rice from EMS-mutated populations [J]. J. Nucl. Agric. Sci., 2021,35(2):253-261. | |
77 | 范方军, 王芳权, 李文奇, 等.抗咪草烟水稻资源的筛选[J]. 中国稻米,2018,24(6):108-109. |
FAN F J, WANG F Q, LI W Q, et al.. Screening of imazethapyr-resistant rice resources [J]. China Rice, 2018,24(6):108-109. | |
78 | TUINSTRA M R, AL-KHATIB K. Acetyl-CoA carboxylase herbicide resistant sorghum [P]. USA: US20100293628,2010-11-18. |
79 | LIU X, QIN R, LI J, et al.. A CRISPR-Cas9- mediated domain-specifific base-editing screen enables functional assessment of ACCase variants in rice [J]. Plant Biotechnol. J., 2020,18(9):1845-1847. |
80 | LI C, ZHANG R, MENG X, et al.. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors [J]. Nat. Biotechnol., 2020,38(7):875-882. |
81 | HINGA M, GRIFFIFIN S M, MOON S, et al.. Methods and compositions to produce rice resistant to accase inhibitors [P]. WO patent, WO2013016210, 2015-04-22. |
82 | LINSCOMBE S D. Rice cultivar designated 'PVL 01' [P]. US patent, US20200196551A1, 2018-12-27. |
[1] | Liuxi YI, Rula SA, Xin FAN, Can ZHAO, Ru LI, Bateer SIQIN. Evaluation of Flax Germplasm Phenotype [J]. Journal of Agricultural Science and Technology, 2022, 24(5): 56-67. |
[2] | Junjie ZOU, Miaoyun XU, Lan ZHANG, Yanzhong LUO, Yuan LIU, Hongyan ZHENG, Lei WANG. Molecular Characterizations of Stacked Transgenic Maize BBHTL8-1 with Insect Resistance, Glyphosate Tolerance and Improved Quality [J]. Journal of Agricultural Science and Technology, 2022, 24(2): 77-85. |
[3] | Saiqun WEN, Lingdi LIU, Wei TIAN, Xiaoliang XIE, Dongsheng JIA, Ming LIU, Chunxiu WEN, Tao JIANG. Identification of Perilla Germplasm for Both Medicinal and Edible Leaves [J]. Journal of Agricultural Science and Technology, 2022, 24(10): 62-70. |
[4] | Junjie ZOU, Miaoyun XU, Lan ZHANG, Hongyan ZHENG, Lei WANG. Molecular Characteristics and Functional Evaluation of Transgenic Maize BFL4-1 With Stacked Insect and Herbicide Resistance Traits [J]. Journal of Agricultural Science and Technology, 2022, 24(1): 31-37. |
[5] | WANG Ping. The Main Problems and Countermeasures of Seed Industry Development in China [J]. Journal of Agricultural Science and Technology, 2021, 23(11): 7-16. |
[6] | LI Jichao, ZHANG Jinyu, YANG Tianmei, YANG Meiquan, YANG Weize, XU Zongliang, ZUO Yingmei*. Comprehensive Evaluation and Physiological Mechanism of Drought Resistance of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz Germplasm Resources [J]. Journal of Agricultural Science and Technology, 2020, 22(10): 49-59. |
[7] | WANG Feiquan1, FENG Hua1, LUO Shengcai2, CHEN Rongbing1, YANG Huizhu1, LI Shaohua1, ZHNAG Jianming3, ZHANG Bo1, YE Jianghua1. Diversity Analysis of Agronomic Traits of Wuyi Mingcong Tea Plant Germplasm Resources [J]. Journal of Agricultural Science and Technology, 2019, 21(6): 43-54. |
[8] | XU Meiling1, HE Xiaohui2, SONG Yuchuan2, FAN Youyin2, LI Yongping1. Correlation Analysis of Routine Chemical Components, Polyphenol and Sensory Quality of Cigar Germplasm Resources [J]. Journal of Agricultural Science and Technology, 2019, 21(6): 124-134. |
[9] | FENG Bin, LI Huilin, YANG Shengxian, LIN Tao, WEI Zhongfen*. Analysis of Genetic Diversity of Sunflower Germpasm Resources in Guizhou [J]. Journal of Agricultural Science and Technology, 2018, 20(7): 34-41. |
[10] | WANG Xiangdong1, MA Yanzhi2*, KE Shaoying2. Effects of Different Herbicides on Yield and Effective Component of Bupleurum chinense [J]. Journal of Agricultural Science and Technology, 2018, 20(4): 132-137. |
[11] | YANG Yang1,2, LIANG Chengzhen2, MENG Zhigang2, CHEN Quanjia1, LUO Shuping1, ZHANG Rui2, GUO Sandui2*. Construction of Insect and Herbicide Resistant Expression Vector Based on GR79 EPSPS Screening Marker and Identification of Resistance [J]. Journal of Agricultural Science and Technology, 2018, 20(11): 22-28. |
[12] | CHEN Li-na1, 2, FANG Wei1, SI Hai-ping3, CAO Yong-sheng1*. Crop Germplasm Resources Data Integration Based on Ontology [J]. Journal of Agricultural Science and Technology, 2016, 18(3): 82-88. |
[13] | WANG Li-hong, LI Hui-bin, SUN Xin-bo, BIAN Xiu-ju. Evaluation and Analysis on Wild Germplasm Resources of Pennisetum alopecuroides [J]. Journal of Agricultural Science and Technology, 2016, 18(3): 134-140. |
[14] | CHENG Liang1,2, GUO Qing\|yun1,2*. Potential Research of Fusarium avenaceum Isolate GD\|2 as a Bioherbicide Agent for Wild Oats(Avena fatua L.) [J]. , 2014, 16(3): 70-80. |
[15] | YAO Wen-hua, HAN Xue-li, WANG Yan-fen, TAN Jing, XU Chun-xia, CHEN Hong-mei, FAN. Research Status and Development Strategy for Sweet Corn Breeding in China [J]. , 2011, 13(2): 1-8. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||