Effect of Exogenous Chlorellavulgaris on Physiological Characteristics of Flue-cured Tobacco Seedlings Under Drought Stress

doi:10.13304/j.nykjdb.2023.0797

Abstract

Abstract:

To study the effect of exogenous Chlorella on physiological characteristics of flue-cured tobacco seedlings under drought stress， the tobacco variety ‘Zhongyan 100’ was as the experimental material. The seedling of flue-cured tobacco was sprayed water as control （CK）， and treated with drought （D）， and was sprayed by 50 （T1）， 100 （T2）， 150 times （T3） of Chlorellavulgaris on the basis of drought treatment. The physiological characteristics of flue-cured tobacco seedlings under different treatments were compared. The results showed that the application of Chlorella vulgaris increased the antioxidant enzyme activity， soluble protein content， photosynthetic characteristics and chlorophyll content of tobacco， and decreased the content of malondialdehyde and proline decreased. T1 treatment had the best effect on promoting growth and development of flue-cured tobacco under drought stress. In conclusion， the application of Chlorella vulgaris could effectively alleviate the impact of drought stress on seedling of flue-cured tobacco.

Key words: Chlorella vulgaris, nitrogen fixation, drought, flue-cured tobacco

摘要：

为研究外源小球藻对干旱胁迫下烤烟幼苗生理特性的影响，以烤烟品种‘中烟100’为试验材料，以喷施清水为对照（CK），分别设置干旱处理（D）及在干旱处理的基础上将小球藻稀释50（T1）、100（T2）、150倍（T3）后施用于烤烟幼苗，对不同处理下烤烟幼苗的生理特性进行比较。结果表明，施用小球藻后，烤烟幼苗的抗氧化酶活性、光合特性及可溶性蛋白和叶绿素含量均得到提升，丙二醛和脯氨酸含量下降，其中T1处理对于缓解干旱胁迫对烤烟的生长发育的效果最佳。综上，施用小球藻能有效缓解干旱胁迫对烤烟幼苗的影响。

关键词: 小球藻, 固氮, 干旱, 烤烟

CLC Number:

S572

Shenghao ZOU, Qiwei YU, Shuai HE, Xuewei ZHANG, Qian MA, Guankai MA, Feihu XI, Dongsheng LUO, Maoxian WANG, Zhenbao LUO, Yanqiu JING. Effect of Exogenous Chlorellavulgaris on Physiological Characteristics of Flue-cured Tobacco Seedlings Under Drought Stress[J]. Journal of Agricultural Science and Technology, 2025, 27(6): 64-71.

邹声浩, 喻奇伟, 贺帅, 张学伟, 马黔, 马关凯, 席飞虎, 罗东升, 王茂贤, 罗贞宝, 景延秋. 外源小球藻对干旱胁迫下烤烟幼苗生理特性的影响[J]. 中国农业科技导报, 2025, 27(6): 64-71.

Figures/Tables 7

References 24

1	GITAU M M, FARKAS A, BALLA B, et al.. Strain-specific biostimulant effects of Chlorella and Chlamydomonas green microalgae on Medicago truncatula [J/OL]. Plants, 2021, 10(6):1060 [2023-10-08]. .
2	郝俊光,潘喜芳,莫维,等.小球藻作为食品利用的国外报道研究进展[J].中国酿造,2022,41(10):18-24.
	HAO J G, PAN X F, MO W, et al.. Research progress of foreign reports on Chlorella as food utilization [J]. Chin Brew., 2022,41(10):18-24.
3	任超杰,钟丹妮,周民.微藻在生物医学领域的研究进展[J].浙江大学学报(医学版),2021,50(2):261-266.
	REN C J, ZHONG D N, ZHOU M. Research progress on the biomedical application of microalgae [J]. Zhejiang Univ. (Med. Sci.), 2021, 50(2):261-266.
4	元文霞,毕影东,樊超,等.我国生物肥料的发展现状与应用[J].农业科技通讯,2022(12):4-9.
5	边建文,崔岩,杨宋琪,等.微藻生物肥料的农业应用研究进展[J].中国土壤与肥料,2020(5):1-9.
	BIAN J W, CUI Y, YANG S Q, et al.. Research progress in agricultural application of microalgae bio-fertilizer [J]. Soil Fert. Sci. China, 2020(5):1-9.
6	LU Y D, XU J. Phytohormones in microalgae:a new opportunity for microalgal biotechnology [J]. Trends Plant Sci., 2015, 20(5):273-282.
7	HUSSAIN H I, KASINAdHUNI N, ARIOLIT. The effect of seaweed extract on tomato plant growth, productivity and soil [J]. J. Appl. Phycol., 2021, 332:1305-1314.
8	RASHAD Y M, EL-SHARKAWY H H, ELAZAB N T. Ascophyllum nodosum extract and mycorthizalcolonization synergistically trigger immune responses in pea plants against rhizoctonia root rot, and enhanceplant growth and productivity [J/OL]. J. Fungi, 2022, 8(3):268 [2023-10-08]. .
9	张继红,任丹丹,姜玉声,等.微藻营养价值及其在水产生物营养强化中的应用[J].食品工业科技,2016,37(20):371-376.
	ZHANG J H, REN D D, JIANG Y S, et al.. Microalgae in aquaculture: a review to nutritional value and rotifers enrichment [J]. Sci. Technol. Food Ind., 2016, 37(20):371-376.
10	邱智超,勾宇春,张志鹏,等.藻类资源在农业种植业中的应用研究进展[J].农业资源与环境学报,2023,40(4):840-851.
	QIU Z C, GOU Y C, ZHANG Z P, et al.. Research progress in the application of algal resources in agriculture:a review [J]. J. Agric. Resour. Environ., 2023, 40(4):840-851.
11	熊斯微,范泽,张海涛,等.小球藻的营养价值及其在水产动物饲料中的应用[J].饲料工业,2024,45(6):7-13.
	XIONG S W, FAN Z, ZHANG H T, et al.. Nutritional value and applications in aquatic animal feed of Chlorella [J]. Feed Ind., 2024, 45(6):7-13.
12	EL-BAKY H H A, EL-BAZ F K, BAROTY G S E. Enhancing antioxidant availability in wheat grains from plants grown under seawater stress in response to microalgae extract treatments [J]. J. Sci. Food Agric., 2010, 90(2):299-303.
13	OANCEA F, VELEA S, FATU V, et al.. Micro-algae based plant biostimulant and its effect on water stressed tomato plants [J]. Romanian J. Plant Prot., 2013, 6:104-117.
14	代晓燕,陈培钰,李子绅,等.不同钾水平下外源铵对烟草幼苗根系生长及钾吸收的影响[J].中国土壤与肥料,2023(9):120-127.
	DAI X Y, CHEN P Y, LI Z S, et al.. Effects of exogenous ammonium on root growth and potassium uptake of tobacco under different potassium concentrations [J]. Soil Fert. Sci. China, 2023(9):120-127.
15	卢军,钱宇,杨柳,等.低温胁迫对烟草种子活力和理化特性的影响[J].种子科技,2022,40(10):4-6.
16	蒋伟峰,金佳威,常剑波,等.根施γ-聚谷氨酸对烤烟根系活力及烟叶产量和品质的影响[J].西北农林科技大学学报(自然科学版),2023,51(7):56-64.
	JIANG W F, JIN J W, CHANG J B, et al.. Effects of γ-poly glutamic acid on root activity, yield and quality of flue-cured tobacco [J]. J. Northwest A&F Univ. (Nat. Sci.), 2023, 51(7):56-64.
17	萧浪涛,王三根.植物生理学实验技术[M].北京:中国农业出版社,2005:1-262.
18	王亚虹,许自成,高森,等.烟草干旱胁迫研究进展[J].节水灌溉,2016(12):103-107, 111.
	WANG Y H, XU Z C, GAO S, et al.. Research progress of tobacco drought stress [J]. Water Saving Irrig., 2016(12):103-107, 111.
19	李佳,刘涛,马菊莲,等.烟草响应干旱胁迫与抗旱遗传育种研究进展[J].江苏农业科学,2023,51(8):34-43.
	LI J, LIU T, MA J L, et al.. Research progress on drought stress response and drought resistance genetic breeding of tobacco [J]. Jiangsu Agric. Sci., 2023, 51(8):34-43.
20	魏文志,夏文水,吴玉娟.小球藻糖蛋白的分离纯化与抗氧化活性评价[J].食品与机械,2006(5):20-22.
	WEI W Z, XIA W S, WU Y J. Isolation purification and anti-oxidation of the glycoprotein from Chlorella pyrenoidosa [J]. Food Mach., 2006(5):20-22.
21	邓帅涛. 小球藻生物肥制备体系优化及其对小麦的促生作用[D].太原:山西农业大学,2022.
	DENG S T. Optimization of preparation system of Chlorella sorokiniana DT01 biofertilizer and its growth promoting effect on wheat [D]. Taiyuan: Shanxi Agricultural University, 2022.
22	王丽君,李冬,申洪涛,等.油菜素内酯对干旱胁迫下烤烟幼苗生长生理及光合特性的影响[J].西北农林科技大学学报(自然科学版),2020,48(11):33-41.
	WANG L J, LI D, SHEN H T, et al.. Effects of brassinolide on growth, physiology and photosynthetic characteristics of flue-cured tobacco seedlings under drought stress [J]. J. Northwest A&F Univ. (Nat. Sci.), 2020, 48(11):33-41.
23	张靖洁. 微藻生物肥改善土壤肥力及根际菌群和提高小油菜产量品质效应的研究[D].太原:山西农业大学,2022.
	ZHANG J J. Liquid microalgal biofertilizer enhance yield and quality of Brassica chinensis by improving soil fertility and rhizosphere flora [D]. Taiyuan: Shanxi Agricultural University, 2022.
24	李岩.小球藻诱导植物气孔闭合的机制及其抗蒸腾效应研究[D].杭州:浙江大学,2017.
	LI Y. The mechanisms underlying Chlorella-induced stomatal closure and its anti-transpiration effect in plants [D]. Hangzhou: Zhejiang University, 2017.

胁迫时间Stress time/d	处理 Treatment	净光合速率P_n/ （μmol CO₂·m^-2·s^-1）	气孔导度G_s/ （mmol·m^-2·s^-1）	胞间CO₂浓度C_i/ （μL·L^-1）	蒸腾速率T_r/ （mmol·m^-2·s^-1）
0	CK	12.68±0.95 a	0.38±0.26 a	146.63±4.13 b	4.36±1.17 bc
	D	11.41±0.68 a	0.37±0.03 a	149.75±14.73 a	4.53±0.59 b
	T1	11.58±1.06 a	0.33±0.12 a	141.43±28.54 c	4.72±1.36 a
	T2	13.31±3.13 a	0.36±0.24 a	139.76±19.48 cd	4.23±1.73 c
	T3	11.93±1.47 a	0.35±0.07 a	139.47±18.09 d	4.47±1.51 b
5	CK	16.13±0.45 a	0.65±0.05 a	151.27±30.55 e	6.12±0.87 a
	D	8.03±0.58 d	0.57±0.01 b	211.06±25.63 a	5.59±0.49 b
	T1	12.04±3.63 b	0.32±0.12 c	175.45±14.95 d	4.63±1.84 b
	T2	10.67±0.85 bc	0.36±0.08 c	184.53±15.71 c	3.86±0.83 c
	T3	9.53±2.86 cd	0.41±0.05 c	196.45±22.81 b	3.49±1.04 d
10	CK	19.56±2.41 a	0.89±0.06 a	175.92±26.55 e	9.23±2.66 a
	D	11.28±1.09 d	0.64±0.02 b	243.84±12.82 a	7.58±0.85 b
	T1	16.22±1.52 b	0.53±0.14 c	197.73±61.48 d	7.46±2.81 b
	T2	14.48±0.65 c	0.60±0.23 bc	213.80±30.46 c	6.85±0.93 c
	T3	13.83±3.40 c	0.69±0.18 b	226.12±20.83 b	6.53±1.06 d
15	CK	23.43±0.72 a	1.03±0.26 a	229.06±34.61 e	12.88±2.69 a
	D	14.52±1.02 d	0.85±0.01 ab	286.49±16.49 a	9.69±0.52 b
	T1	20.20±2.58 b	0.74±0.23 b	239.15±19.68 d	8.36±1.75 bc
	T2	18.81±2.96 bc	0.79±0.20 b	254.19±25.05 c	7.93±0.91 bc
	T3	17.23±1.28 c	0.86±0.17 ab	266.81±37.51 b	7.24±3.15 c

胁迫时间Stress time/d	处理 Treatment	净光合速率P_n/ （μmol CO₂·m^-2·s^-1）	气孔导度G_s/ （mmol·m^-2·s^-1）	胞间CO₂浓度C_i/ （μL·L^-1）	蒸腾速率T_r/ （mmol·m^-2·s^-1）
0	CK	12.68±0.95 a	0.38±0.26 a	146.63±4.13 b	4.36±1.17 bc
	D	11.41±0.68 a	0.37±0.03 a	149.75±14.73 a	4.53±0.59 b
	T1	11.58±1.06 a	0.33±0.12 a	141.43±28.54 c	4.72±1.36 a
	T2	13.31±3.13 a	0.36±0.24 a	139.76±19.48 cd	4.23±1.73 c
	T3	11.93±1.47 a	0.35±0.07 a	139.47±18.09 d	4.47±1.51 b
5	CK	16.13±0.45 a	0.65±0.05 a	151.27±30.55 e	6.12±0.87 a
	D	8.03±0.58 d	0.57±0.01 b	211.06±25.63 a	5.59±0.49 b
	T1	12.04±3.63 b	0.32±0.12 c	175.45±14.95 d	4.63±1.84 b
	T2	10.67±0.85 bc	0.36±0.08 c	184.53±15.71 c	3.86±0.83 c
	T3	9.53±2.86 cd	0.41±0.05 c	196.45±22.81 b	3.49±1.04 d
10	CK	19.56±2.41 a	0.89±0.06 a	175.92±26.55 e	9.23±2.66 a
	D	11.28±1.09 d	0.64±0.02 b	243.84±12.82 a	7.58±0.85 b
	T1	16.22±1.52 b	0.53±0.14 c	197.73±61.48 d	7.46±2.81 b
	T2	14.48±0.65 c	0.60±0.23 bc	213.80±30.46 c	6.85±0.93 c
	T3	13.83±3.40 c	0.69±0.18 b	226.12±20.83 b	6.53±1.06 d
15	CK	23.43±0.72 a	1.03±0.26 a	229.06±34.61 e	12.88±2.69 a
	D	14.52±1.02 d	0.85±0.01 ab	286.49±16.49 a	9.69±0.52 b
	T1	20.20±2.58 b	0.74±0.23 b	239.15±19.68 d	8.36±1.75 bc
	T2	18.81±2.96 bc	0.79±0.20 b	254.19±25.05 c	7.93±0.91 bc
	T3	17.23±1.28 c	0.86±0.17 ab	266.81±37.51 b	7.24±3.15 c

胁迫时间 Stress time/d	处理 Treatment	叶绿素a含量 Chl a content/（mg·g^-1）	叶绿素b含量 Chl b content/（mg·g^-1）	类胡萝卜素含量 Car content/（mg·g^-1）
0	CK	1.74±0.51 a	0.67±0.15 a	0.39±0.07 a
	D	1.03±0.68 c	0.31±0.03 b	0.20±0.03 a
	T1	1.12±0.39 c	0.37±0.07 b	0.22±0.05 a
	T2	1.38±0.31 b	0.44±0.12 b	0.26±0.02 a
	T3	1.54±0.43 b	0.49±0.17 ab	0.29±0.04 a
5	CK	4.91±0.93 a	1.28±0.56 a	0.68±0.07 a
	D	2.87±0.67 d	0.83±0.56 b	0.41±0.10 b
	T1	3.55±0.78 b	0.88±0.41 b	0.57±0.12 ab
	T2	3.26±1.13 c	0.97±0.33 b	0.50±0.08 ab
	T3	3.03±1.05 d	1.15±0.28 a	0.45±0.06 b
10	CK	7.35±1.86 a	2.95±1.04 a	0.87±0.32 a
	D	5.17±2.04 e	1.56±0.38 e	0.55±0.09 bc
	T1	6.22±1.31 b	2.53±0.43 b	0.71±0.06 ab
	T2	5.98±1.67 c	2.26±0.52 c	0.66±0.23 bc
	T3	5.61±1.74 d	1.89±0.24 d	0.52±0.14 c
15	CK	9.01±1.58 a	3.83±1.21 a	1.60±0.48 a
	D	7.23±1.52 d	2.67±0.36 d	1.08±0.37 d
	T1	7.92±2.07 b	3.25±1.15 b	1.39±0.25 b
	T2	7.67±1.39 c	3.03±1.09 c	1.26±0.35 bc
	T3	7.21±2.12 d	2.83±0.57 d	1.17±0.19 cd

胁迫时间 Stress time/d	处理 Treatment	叶绿素a含量 Chl a content/（mg·g^-1）	叶绿素b含量 Chl b content/（mg·g^-1）	类胡萝卜素含量 Car content/（mg·g^-1）
0	CK	1.74±0.51 a	0.67±0.15 a	0.39±0.07 a
	D	1.03±0.68 c	0.31±0.03 b	0.20±0.03 a
	T1	1.12±0.39 c	0.37±0.07 b	0.22±0.05 a
	T2	1.38±0.31 b	0.44±0.12 b	0.26±0.02 a
	T3	1.54±0.43 b	0.49±0.17 ab	0.29±0.04 a
5	CK	4.91±0.93 a	1.28±0.56 a	0.68±0.07 a
	D	2.87±0.67 d	0.83±0.56 b	0.41±0.10 b
	T1	3.55±0.78 b	0.88±0.41 b	0.57±0.12 ab
	T2	3.26±1.13 c	0.97±0.33 b	0.50±0.08 ab
	T3	3.03±1.05 d	1.15±0.28 a	0.45±0.06 b
10	CK	7.35±1.86 a	2.95±1.04 a	0.87±0.32 a
	D	5.17±2.04 e	1.56±0.38 e	0.55±0.09 bc
	T1	6.22±1.31 b	2.53±0.43 b	0.71±0.06 ab
	T2	5.98±1.67 c	2.26±0.52 c	0.66±0.23 bc
	T3	5.61±1.74 d	1.89±0.24 d	0.52±0.14 c
15	CK	9.01±1.58 a	3.83±1.21 a	1.60±0.48 a
	D	7.23±1.52 d	2.67±0.36 d	1.08±0.37 d
	T1	7.92±2.07 b	3.25±1.15 b	1.39±0.25 b
	T2	7.67±1.39 c	3.03±1.09 c	1.26±0.35 bc
	T3	7.21±2.12 d	2.83±0.57 d	1.17±0.19 cd

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[13]	Yongjin LIANG, Ruixuan ZHU, Beilei WEI, Xiaomai YUAN, Wuyang CHENG, Bo PENG, Ziting WANG, Jianyu WEI. Effect of Combined Application of Organic Fertilizer on Tobacco Leaf Quality in China： a Meta-analysis [J]. Journal of Agricultural Science and Technology, 2024, 26(12): 164-175.
[14]	Yuan HE, Xiaotong GU, Liqing FENG, Huijun DUAN, Yongsheng TAO. Screening and Evaluation of Drought Resistance Index for Maize Hybrids During Seedling and Germination Stages [J]. Journal of Agricultural Science and Technology, 2024, 26(10): 30-40.
[15]	Rui TIAN, Hua ZHANG, Meihong HUANG, Zhenqi SHAO, Xihuan LI, Caiying ZHANG. Mining of Candidate Genes and Genetic Loci Conferring Drought Tolerance in Soybean [J]. Journal of Agricultural Science and Technology, 2023, 25(9): 69-82.