Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (9): 69-82.DOI: 10.13304/j.nykjdb.2022.1035

• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles     Next Articles

Mining of Candidate Genes and Genetic Loci Conferring Drought Tolerance in Soybean

Rui TIAN(), Hua ZHANG, Meihong HUANG, Zhenqi SHAO, Xihuan LI(), Caiying ZHANG()   

  1. North China Key Laboratory for Crop Germplasm Resources of Education Ministry,College of Agronomy,Hebei Agricultural University,Hebei Baoding 071001,China
  • Received:2022-11-27 Accepted:2022-12-26 Online:2023-09-15 Published:2023-09-28
  • Contact: Xihuan LI,Caiying ZHANG

大豆抗旱遗传位点及候选基因发掘

田蕊(), 张华, 黄玫红, 邵振启, 李喜焕(), 张彩英()   

  1. 河北农业大学农学院,教育部华北作物种质资源研究与利用重点实验室,河北 保定 071001
  • 通讯作者: 李喜焕,张彩英
  • 作者简介:田蕊 E-mail:tianrui7726@163.com
  • 基金资助:
    现代农业科技奖励性后补助资金项目(17927670H);河北省现代农业产业技术体系创新团队项目(HBCT2019190203)

Abstract:

Chlorophyll is an essential pigment for plant photosynthesis. Drought tolerance affects chlorophyll synthesis, reduces photosynthetic efficiency, and decreases soybean yield. In order to explore the genetic loci of chlorophyll content in soybean under drought stress, drought resistance index (DRI) were calculated by chlorophyll contents under drought and well-watered conditions. Moreover, based on the DRI and re-sequenced genotypes of the soybean natural population (199 accessions) at 20× sequencing depth, the genome-wide association study (GWAS) was performed. The results showed that 237 single nucleotide polymorphisms(SNPs) in 18 loci on the chromosomes 1, 2, 3, 7, 10, 16, 17 and 18 were identified to associate with DRI. Moreover, 181 SNPs (76.37% of the total) were located on chromosome 16, of which 9 SNPs were located in the gene introns, 17 in exons, 21 in upstream or downstream, and 1 in 3’UTR. Based on these, 57 candidate genes flanking the associated SNPs were found, and according to the gene annotation, SNP mutation, and RNA expression, 3 candidate genes, Glyma.16G063600 (F-box protein), Glyma.10G007000 (AP2-EREBP), and Glyma.17G143900 (AP2/ERF) were screened out which might be responsible for the drought tolerance in soybean. Above results provided important theoretical foundation for drought tolerance genetic improvement in soybean.

Key words: soybean, chlorophyll content, drought stress, GWAS

摘要:

叶绿素是植物光合作用必需色素,干旱胁迫可影响其合成并降低光合效率,进而影响大豆产量。为发掘有关大豆干旱胁迫叶绿素含量遗传位点,以199份大豆品种资源构建的自然群体为材料,测定其干旱及正常条件下的叶绿素含量,计算抗旱指数(drought resistance index, DRI),并依据抗旱指数和20×重测序基因型进行全基因组关联分析(genome-wide association study, GWAS)。结果表明,共定位到237个显著关联单核苷酸多态性(single nucleotide polymorphism,SNP)标记(划分为18个位点),分别位于1、2、3、7、10、16、17及18号染色体。在16号染色体关联到181个SNPs,占总SNPs的76.37%,属热点区域,其中9个位于基因内含子区,17个位于外显子区,11个位于上游,10个位于下游,1个位于3’UTR。基于显著关联SNPs获得57个基因,依据基因注释、DNA水平SNP等位变异、RNA水平表达模式,对57个基因进行分析,筛选出3个与大豆抗旱指数相关基因,其中Glyma.16G063600编码F-box蛋白,Glyma.10G007000编码AP2-EREBP转录因子,Glyma.17G143900编码AP2/ERF结构域蛋白。以上研究结果为大豆抗旱育种奠定理论基础。

关键词: 大豆, 叶绿素含量, 干旱胁迫, 全基因组关联分析

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