Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (1): 50-57.DOI: 10.13304/j.nykjdb.2021.0843
• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles
Zhanwu YANG(), Hui DU, Xinzhu XING, Wenlong LI, Youbin KONG, Xihuan LI, Caiying ZHANG(
)
Received:
2021-09-28
Accepted:
2021-12-04
Online:
2023-01-15
Published:
2023-04-17
Contact:
Caiying ZHANG
杨占武(), 杜汇, 邢馨竹, 李文龙, 孔佑宾, 李喜焕, 张彩英(
)
通讯作者:
张彩英
作者简介:
杨占武 E-mail:1091748213@qq.com;
基金资助:
CLC Number:
Zhanwu YANG, Hui DU, Xinzhu XING, Wenlong LI, Youbin KONG, Xihuan LI, Caiying ZHANG. Functional Analysis of Cytochrome P450 Family GmCYP78A71 in Soybean Nodulation[J]. Journal of Agricultural Science and Technology, 2023, 25(1): 50-57.
杨占武, 杜汇, 邢馨竹, 李文龙, 孔佑宾, 李喜焕, 张彩英. 大豆细胞色素P450家族GmCYP78A71固氮功能解析[J]. 中国农业科技导报, 2023, 25(1): 50-57.
引物名称 Primer name | 引物序列 Primer sequence (5’-3’) |
---|---|
Promoter-F | CCCCTGCAGGGCAATGAAAATAATGCCATGTC |
Promoter-R | CCCGGATCCGATTAGGAGCACGTAAGATGATTC |
RT-F | TGGTTGGTGAAATGGTGATG |
RT-R | CCTCTACCACCCCTTTGTCC |
ORF-F | CCTCTAGAATGATTCCAACACTTGTTTGTATTG |
ORF-R | AAGGATCCTCAAGAGCTTATGGTGTTGAACCTG |
RNAi-F | GGGGTACCACTAGTATTGGCGCCACCTACGTAAA |
RNAi-R Actin11-F Actin11-R | GGGGATCCGAGCTCCCCAACGCCTCCTTTGTTTG ATCTTGACTGAGCGTGGTTATTCC GCTGGTCCTGGCTGTCTCC |
Table 1 Primers sused in this study
引物名称 Primer name | 引物序列 Primer sequence (5’-3’) |
---|---|
Promoter-F | CCCCTGCAGGGCAATGAAAATAATGCCATGTC |
Promoter-R | CCCGGATCCGATTAGGAGCACGTAAGATGATTC |
RT-F | TGGTTGGTGAAATGGTGATG |
RT-R | CCTCTACCACCCCTTTGTCC |
ORF-F | CCTCTAGAATGATTCCAACACTTGTTTGTATTG |
ORF-R | AAGGATCCTCAAGAGCTTATGGTGTTGAACCTG |
RNAi-F | GGGGTACCACTAGTATTGGCGCCACCTACGTAAA |
RNAi-R Actin11-F Actin11-R | GGGGATCCGAGCTCCCCAACGCCTCCTTTGTTTG ATCTTGACTGAGCGTGGTTATTCC GCTGGTCCTGGCTGTCTCC |
Fig. 1 Sequence analysis of GmCYP78A71 and expression of CYP78A familyA: Genome sequence of GmCYP78A71; B:GmCYP78A71 protein domain analysis; C: Expression analysis of CYP78A family
Fig. 2 Relative expression level of GmCYP78A71A: Expression levels of GmCYP78A71 in different organs; B: Expression levels of GmCYP78A71 in different developmental stages of nodule
Fig. 4 Phenotype and biological function analysis of nodules of GmCYP78A71 transgenic complex plantA: Phenotype of nodules; B: Relative expression level of GmCYP78A71; C: Nitrogenase activity; D: Nodule number; E: Nodule fresh weight. OX—Overexpression lines; WT—Wild type; RNAi—RNAi lines
1 | ZHAO B T, DAI A H, WEI H C, et al.. Arabidopsis KLU homologue GmCYP78A72 regulates seed size in soybean [J]. Plant Mol. Biol., 2016, 90(1-2): 33-47. |
2 | 杨红旗,郝仰坤.我国大豆产业回顾、现状与发展对策[J].广东农业科学, 2010, 37(1):188-191. |
YANG H Q, HAO Y K. Histroical review current situation and development countermeasure of Chinese soybean [J]. Guangdong Agric. Sci., 2010, 37(1): 188-191. | |
3 | 姚玉波,马春梅,张磊,等.施氮水平对大豆吸收利用氮素及产量的影响[J].东北农业大学学报. 2009, 40(4): 6-10. |
YAO Y B, MA C M, ZHANG L, et al.. Effect of nitrogen levels on absorption and utilization and utilization of nitrogen and yield of soybean [J]. J. Northeast Agric. Univ., 2009, 40(4): 6-10. | |
4 | CONLEY D J, PAERL H W, Howarth R W, et al.. Controlling Eutrophication: Nitrogen and Phosphorus [J]. Science, 2009, 323(5917): 1014-1015. |
5 | TILMAN D, FARGIONE J, WOLFF B, et al.. Forecasting agriculturally driven global environmental change [J]. Science, 2001, 292(5515): 281-284. |
6 | KIERS E T, HUTTON M G, DENISON R F. Human selection and the relaxation of legume defences against ineffective rhizobia [J]. Proc. Biol. Soc., 2007, 274(1629): 3119-3126. |
7 | YAN A M, WANG E T, KAN F L, et al.. Sinorhizobium meliloti associated with Medicago sativa and Melilotus spp. in arid saline soils in Xinjiang, China. [J]. Int. J. Syst. Evol. Microbiol., 2000, 50(5): 1887-1891. |
8 | PROVOROV N A, TIKHONOVICH I A. Genetic resources for improving nitrogen fixation in legume-rhizobia symbiosis [J]. Genet. Resour. Crop Evol., 2003, 50(1): 89-99. |
9 | 慈恩,高明.生物固氮的研究进展[J].中国农学通报, 2004, 20(1): 25-28. |
CI E, GAO M. Research progress on biological nitrogen fixation [J]. Chin. Agric. Sci. Bull., 2004, 20(1): 25-28. | |
10 | 关大伟,李力,岳现录,等.我国大豆的生物固氮潜力研究[J].植物营养与肥料学报, 2014, 2(6): 1497-1504. |
GUAN D W, LI L I, YUE X L, et al.. Study on potential of biological nitrogen fixation of soybean in China [J]. J. Plant Nutr. Fert., 2014, 20(6): 1497-1504. | |
11 | NEBERT D W, NELSON D R, ADESNIK M, et al.. The P450 superfamily: updated listing of all genes and recommended nomenclature for the chromosomal loci [J]. DNA, 1989, 8(1): 1-13. |
12 | NELSON D, WERCK-REICHHART D. A P450-centric view of plant evolution [J]. Plant J., 2011, 66(1): 194-211. |
13 | RENSING S A, LANG D, ZIMMER A D, et al.. The physcomitrella genome reveals evolutionary insights into the conquest of land by plants [J]. Science, 2008, 319(5859): 64-69. |
14 | NELSON D R. Plant cytochrome P450s from moss to poplar [J]. Phytochem. Rev., 2006, 5(2-3): 193-204. |
15 | ANASTASIOU E, KENZ S, GERSTUNG M, et al.. Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling [J]. Dev. Cell, 2007, 13(6): 843-856. |
16 | FANG W J, WANG Z B, CUI R F, et al.. Maternal control of seed size by EOD3/CYP78A6 in Arabidopsis thaliana [J]. Plant J., 2012, 70(6). 929-939. |
17 | MAEDA S, DUBOUZET J G, KONDOU Y, et al.. The rice CYP78A gene BSR2 confers resistance to Rhizoctonia solani and affects seed size and growth in Arabidopsis and rice [J/OL]. Sci. Rep., 2019, 9(1), 587 [2021-08-10]. . |
18 | ZONDLO S C, IRISH V F. CYP78A5 encodes a cytochrome P450 that marks the shoot apical meristem boundary in Arabidopsis [J]. Plant J., 1999, 19(3): 259-268. |
19 | TAKASHI N, MISAKI K, HIROAKI U, et al.. Highly pleiotropic functions of CYP78As and AMP1 are regulated in non-cell-autonomous/organ-specific manners [J]. Plant Physiol., 2021, 186(1): 767-781. |
20 | KATSUMATA T, FUKAZAWA J, MAGOME H, et al.. Involvement of the CYP78A subfamily of cytochrome P450 monooxygenases in protonema growth and gametophore formation in the moss Physcomitrella patens [J]. Biosci. Biotechnol. Biochem., 2011, 75(2): 331-336. |
21 | DAI A H, YANG S X, ZHOU H K, et al.. Evolution and expression divergence of the CYP78A subfamily genes in soybean [J/OL]. Genes, 2018, 9(12): 611 [2021-08-10]. . |
22 | GUTTIKONDA S K, TRUPTI J, BISHT N C, et al.. Whole genome co-expression analysis of soybean cytochrome P450 genes identifies nodulation-specific P450 monooxygenases [J]. BMC Plant Biol., 2010, 10(1): 243-243. |
23 | KENNETH J L, THOMAS D S. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J]. Methods, 2000, 25(4): 402-408. |
24 | 邢馨竹,杨占武,孔佑宾,等.大豆类胡萝卜素裂解双加氧酶GmCCD8固氮功能解析[J].中国农业科技导报,2022,24(1):46-53. |
XING X Z, YANG Z W, KONG Y B, et al.. Fuctional analysis of carotenoid cleavage dioxygenases GmCCD8 in soybean nodulation [J]. J. Agric. Sci. Technol., 2022,24(1):46-53. | |
25 | ZHAO Y J, CHENG Q Q, SU P, et al.. Research progress relating to the role of cytochrome P450 in the biosynthesis of terpenoids in medicinal plants [J]. Appl. Microbiol. Biotechnol., 2014, 98(6): 2371-2383. |
26 | HULL A K, VIJ R, CELENZA J L. Arabidopsis cytochrome P450s that catalyze the first step of tryptophan-dependent indole-3-acetic acid biosynthesis [J]. Proc. Natl. Acad. Sci. USA, 2000, 97(5): 2379-2384. |
27 | QI X L, LIU C L, SONG L L, et al.. PaCYP78A9, a cytochrome P450, regulates fruit size in sweet cherry (Prunus avium L.) [J/OL]. Front. Plant Sci., 2017, 8:2076 [2021-08-10]. . |
28 | HAYASHI S, REID D E, LORENC M T, et al.. Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots [J]. Plant Biotechnol. J., 2012, 10(8): 995-1010. |
29 | APPLEBY C A, TURNER G L, Macnicol P K. Involvement of oxyleghaemoglobin and cytochrome P-450 in an efficient oxidative phosphorylation pathway which supports nitrogen fixation in Rhizobium [J]. Biochim. Biophys. Acta, 1975, 387(3): 461-474. |
30 | JUNG W, YU O, LAU S M C, et al.. Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes [J]. Nat. Biotechnol., 2000, 18(2): 208-212. |
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