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    15 June 2025, Volume 27 Issue 6
    AGRICULTURAL INNOVATION FORUM
    A Review of Application of Federated Learning in Smart Agriculture Systems
    Minrui TANG, Liang HE, Shenghao GU, Wanxia YANG, Ruijun YUE, Yi TAN, Lei WANG, Tengfei FENG
    2025, 27(6):  1-15.  DOI: 10.13304/j.nykjdb.2024.0002
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    As information technology advances, the collection, processing, analysis and application of agricultural data have become the primary driving force of smart agriculture. In traditional smart agricultural management systems, it is usually required to centralize agricultural data on a central server for analysis and model training, which often poses the risk of data leakage. The leakage of key agricultural privacy data seriously affects the interests of farmers and agricultural institutions, so many the farmers and institutions will carefully handle the issue of sharing original data. To address this issue, federated learning allows different agricultural institutions, farms and agricultural enterprises to complete the training of farming decision models under the condition of only sharing encrypted models, reducing the risk of agricultural privacy data leakage and protecting the legitimate rights and interests of data providers. The theoretical development, technological innovation and practical application of federated learning technology in the field of smart agriculture were introduced. Based on the development trend of smart agriculture systems, it proposed design suggestions for a smart agriculture system based on federated learning. This paper provided references for researchers and practitioners in related fields, offering theoretical value and practical guidance for advancing agricultural data science, ensuring agricultural data security and enhancing the level of agricultural intelligence.

    Spatiotemporal Characteristics and Their Influencing Factors of Crop Diversification in China
    Weiming WANG, Xin PAN, Deping KONG, Yu AN, Shuang GUO, Zhimei SUN, Cheng XUE, Rongjun SUN, Wenqi MA, Huasen XU
    2025, 27(6):  16-27.  DOI: 10.13304/j.nykjdb.2024.0086
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    Intensive production has resulted in a gradual simplification of crop cultivation, but there is a lack of research on quantifying the spatial and temporal variation of crop diversification and evaluating their influencing factors in China. Based on multivariate data about crop production, arable land utilization, socio-economic and production technology from 1949 to 2021 in China, the spatiotemporal characteristics of crop diversification and their driving factors were quantitatively analyzed using generalized likelihood ratio test statistic and random forest model. The results showed that the crop diversification index (CDI) and grain crop diversification index (GCDI) across China showed several significant turning points in history, in which the highest values both were the period from 1991 to 2005. The CDI and GCDI were the greatest in the Sichuan basin and its surrounding areas, and the lowest in South China. On the national scale, the per capita disposable income of rural residents (OECDRR) accounted for the greatest percentage of identified contributing factors for the CDI and GCDI, by 21.0% and 27.5%, respectively. On the regional scale, the cereal scale advantage index,the legume scale advantage index and OECDRR were categorized as the paramount factor for the CDI and GCDI in the Northeast Plain Region. The cereal scale advantage index and OECDRR were the most common of the identified contributing factors for the CDI and GCDI in the Arid and Semi-arid Northern Region and the Huang-Huai-Hai Plain Region. In the other 6 agricultural regions, the CDI and GCDI were obviously driven by the OECDRR. Therefore, it was necessary for regulating planting structure in China to relocate the agricultural functions about crop production and optimize the cultivation area of cereals and legumes, according to the reality of the rural economy,which would consolidate the foundation of food security, promote the green development of agriculture and improve the ability of agriculture to cope with climate change.

    BIOTECHNOLOGY & LIFE SCIENCE
    Genome-wide Identification and Tissue Expression Pattern Analysis of HSP70 Gene Family in Thinopyrum elongatum
    Jilin SUN, Jiaqi ZHANG, Fansen MENG, Silong SUN
    2025, 27(6):  28-38.  DOI: 10.13304/j.nykjdb.2024.0409
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    Heat shock protein 70 (HSP70) constitutes a highly conserved and potent protein family. However, the investigation of HSP70 in Thinopyrum elongatum has not been documented. To identify and study HSP70 gene family in Thinopyrum elongatumTeHSP70 genes were identified based on the systematic application of bioinformatics methods and their expression patterns under salt stress and in different tissues were systematically analyzed. The results showed that a total of 29 TeHSP70 genes were identified in Thinopyrum elongatum, which was categorized into 3 subfamilies and evenly distributed across 7 chromosomes. There were 26 TeHSP70 genes localized in cytoplasm, and all 29 TeHSP70 genes included alpha-helix, extended strand, beta-turn and random coil. The TeHSP70s genes exhibited highly conserved motifs and diverse gene structures, and included multiple hormonal and stress-responsive cis-acting elements in upstream. The collinearity analysis revealed the presence of 3 collinear gene pairs, as well as tandem repeat and segment repeat events, within 29 TeHSP70 genes, and there were numerous homologous gene pairs among Thinopyrum elongatum, rice and wheat, whereas there was a scarcity of such pairs between Thinopyrum elongatum and Arabidopsis. Protein interaction network analysis revealed that there were 11 TeHSP70 genes with a complex and closely protein interaction network. Analysis of expression patterns showed that, with increasing of salt stress (0~300 mmol·L-1), 7 TeHSP70 genes exhibited continuous up-regulation and 6 TeHSP70 genes showed continuous down-regulation, while the remaining 16 members either did not show any expression or displayed inconsistent changes. Similarly, 25 TeHSP70 genes were expressed across all tissues, and 4 TeHSP70 genes were only expressed in some tissues. Above results provided effective references for further analysis of HSP70 genes function of Thinopyrum elongatum.

    Comprehensive Evaluation and Screening of Drought Resistance of 86 Millet Germplasm Resources During Germination Period
    Xinwei XUE, Dan LIU, Shi ZHANG, Wenyu HAN, Ankang MU, Zhikun YU, Fan YANG, Yahui WEN, Jialin ZHANG, Yongping ZHANG, Xianrui WANG
    2025, 27(6):  39-51.  DOI: 10.13304/j.nykjdb.2024.0515
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    The seed germination stage is the most susceptible to external environmental factors. In order to screen millet germplasm resources with strong drought resistance at germination stage, 86 millet germplasms from Inner Mongolia were as materials. 2 treatments were set including PEG-6000 with mass volume fraction of 0% and 20%, 7 traits were determined including germination potential (GP), germination rate (GR), germination index (GI), root length (RL), bud length (BL), root bud ratio (RBR) and fresh weight (FW). The drought resistance of different millet germplasms at germination stage was evaluated by comprehensive evaluation method. The results showed that the variation coefficients of 7 traits were 4.00%~37.44%, and the drought resistance coefficients were 20.06%~33.84%. Correlation analysis showed that there were correlations among the drought resistance coefficients of traits with the highest correlation (0.92) between the germination index and the germination rate. Principal component analysis got 5 principal components with the cumulative contribution rate of 99.25%. The cluster analysis showed that 86 millet germplasms were divided into 5 groups. And 12 millet germplasms were screened with high drought tolerance, including CMG01, CMG04, CMG05, CMG20, CMG30, CMG37, CGK9, GG2, JMK10, CMG58, CMG60, CMG62. The prediction model for evaluating drought resistance of millet germination was developed using stepwise regression analysis, Y=-0.225+0.838x3+0.170x4+0.219x5+0.116x6, which x3x4x5x6 were GI, RL, BL and FW, respectively, and they could be as indicators of drought resistance during the germination period of millet grains. Above results provided the germplasm basis and theoretical reference for breeding new varieties and the drought resistance mechanism of millet.

    Identification and Evaluation of Salt Tolerance in Sorghum at Seedling Stage
    Manhong WANG, Meijuan XIAO, Ahmad IRSHAD, Eltyb Ahmed Nimir NIMIR, Ibrahim El Dessougi HANADI, Guisheng ZHOU, Guanglong ZHU
    2025, 27(6):  52-63.  DOI: 10.13304/j.nykjdb.2024.0616
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    In order to explore the salt tolerance of different sorghum varieties at seedling stage, and screen salt tolerant variety to improve and develop saline soil,19 sorghum varieties were used as materials, and 4 salinity gradients including 0(CK)、100(S1)、150(S2)、200 mmol·L-1 NaCl(S3) were set up to determine germination index and 11 parameters of aboveground and belowground at the germination and seedling stage. The data was analyzed by the analysis of variance, correlation analysis, principal component analysis, affiliation function analysis and cluster analysis to evaluate the salt tolerance of sorghum seedlings. The results showed that the evaluated parameters were significantly affected under salt stress, and the parameters were decreased gradually with salt level increased. Among the treatments, the maximum decreased was showed under S3 treatment. Compared with CK the plant height was decreased by 79.60%, stem diameter by 44.6%, fresh weight of aboveground by 89.97%, dry weigh of aboveground by 62.30%, total root length by 95.65%, root total surface area by 94.56% and root volume of sorghum by 91.97% under S3 treatment. The evaluated 19 sorghum varieties were classified into 4 groups according to the comprehensive salt tolerance coefficient, salt tolerance measure and hierarchical cluster analysis, of which 5 varieties were classified in strong salt tolerance group, another 5 in middle salt tolerance group, 7 varieties in weak salt tolerance group, and 3 varieties in salt sensitive group. Principal component analysis showed that the 3 principal components could represent 78.50% of the original 11 indicators, and root total surface area, dry weight of aboveground and fresh weight of underground could be used as the main indicators in evaluating salt tolerance of sorghum seedlings. The highlight was built a comprehensive salt tolerance evaluation method by integrating aboveground phenotypes and underground root system, which could provide theoretical support for the screening and breeding of salt-tolerant crops.

    Effect of Exogenous Chlorellavulgaris on Physiological Characteristics of Flue-cured Tobacco Seedlings Under Drought Stress
    Shenghao ZOU, Qiwei YU, Shuai HE, Xuewei ZHANG, Qian MA, Guankai MA, Feihu XI, Dongsheng LUO, Maoxian WANG, Zhenbao LUO, Yanqiu JING
    2025, 27(6):  64-71.  DOI: 10.13304/j.nykjdb.2023.0797
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    To study the effect of exogenous Chlorella on physiological characteristics of flue-cured tobacco seedlings under drought stress, the tobacco variety ‘Zhongyan 100’ was as the experimental material. The seedling of flue-cured tobacco was sprayed water as control (CK), and treated with drought (D), and was sprayed by 50 (T1), 100 (T2), 150 times (T3) of Chlorellavulgaris on the basis of drought treatment. The physiological characteristics of flue-cured tobacco seedlings under different treatments were compared. The results showed that the application of Chlorella vulgaris increased the antioxidant enzyme activity, soluble protein content, photosynthetic characteristics and chlorophyll content of tobacco, and decreased the content of malondialdehyde and proline decreased. T1 treatment had the best effect on promoting growth and development of flue-cured tobacco under drought stress. In conclusion, the application of Chlorella vulgaris could effectively alleviate the impact of drought stress on seedling of flue-cured tobacco.

    Analysis of SNP Loci and Alternative Splicing Events in Coffea arabica L. Under Drought Stress
    Haohao YU, Xiangshu DONG, Hao ZHAO, Zhongxian LI, Faguang HU, Yanan LI, Yuqiang LOU, Feifei HE
    2025, 27(6):  72-82.  DOI: 10.13304/j.nykjdb.2023.0903
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    To enrich the information on single nucleotide polymorphism (SNP) loci and elucidate the role of alternative splicing (AS) events in response to drought stress in Coffea arabica L., C. arabica cultivar ‘Catimor’was employed as the experimental material for drought stress treatment for 7 and 14 d, respectively. RNA-seq technology combined with bioinformatics methods was utilized to statistically analyze the drought-resistant SNP loci and AS events in C.arabica. The results showed that a total of 265 047 SNP loci were identified in all the samples, with a transition rate of 60.72% and a transversion rate of 39.28%, distributed on 11 chromosomes, with the most SNPs on Chr. 2. The numbers of SNP loci located in exons and introns of the gene were 110 582 and 100 936, respectively. Sequence annotation revealed that the SNP loci were mainly enriched in amino acid synthesis, transport and carbon metabolism. The results of AS event analysis showed that 3 809 and 4 142 different AS events were identified in the comparison groups of 2 drought stages (0 d of drought vs 7 d of drought; 7 d of drought vs 14 d of drought), respectively. Among them, SE was found to be the most prevalent splicing mode. The genes responsible for these AS events showed significant associations with spliceosome and mRNA surveillance pathway. Through comparative analysis between differential expression genes and AS genes, 4 splicing factors (LOC113699257,LOC113712703, LOC113741326 and LOC113730764) consistently demonstrated significant up-regulation under drought stress conditions. These drought-induced splicing factors might play crucial roles in regulating coffee response to drought stress or root development. Above results laid a foundation for further revealing the molecular mechanism of C.arabica in response to drought stress.

    Identification of Aspergillus tubingensis and the Effect of Gene Editing Methods on Its Homologous Recombination Efficiency
    Licun LIANG, Huimin YU, Xiaochen GUAN, Huoqing HUANG, Bin YAO, Haomeng YANG
    2025, 27(6):  83-92.  DOI: 10.13304/j.nykjdb.2023.0923
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    Aspergillus tubingensis is a filamentous fungus with broad application prospects in feed, food, biological control, environmental restoration and so on. Combining morphological observation with molecular detection, Ehrlich reagent staining, a strain of Aspergillus tubingensis was successfully identified and was named as F316. In order to explore a suitable gene editing method for F316 and facilitate genetic engineering modification, CRISPR/Cas9 was applied to insert hygromycin resistance genes into the spore pigment synthesis related gene fwnA. Through spore color statistics and resistance gene PCR verification, the effects of different delivery methods of Cas9 and gRNA on homologous recombination efficiency were compared. The results showed that, for F316 strain, the homologous recombination efficiency of expression cassette method, self-replicating plasmid method and ribonucleoprotein complex (RNP) method were 70.14%, 66.67% and 25.70%, respectively. In addition, the length of homologous arms in donor DNA also affected the homologous recombination efficiency. When the length of homologous arms was 2 000 bp, the recombination efficiency was the best.

    INTELLIGENT AGRICULTURE & AGRICULTURAL MACHINERY
    Chlorophyll Content Inversion of Spring Wheat Based on Unmanned Aerial Vehicle Hyperspectral and Integrated Learning
    Sile HU, Yulong BAO, Tubuxinbayaer, Jifeng TAO, Enliang GUO
    2025, 27(6):  93-103.  DOI: 10.13304/j.nykjdb.2024.0010
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    Chlorophyll content is a key indicator for monitoring crop growth, and its rapid, effective and accurate estimation is crucial for assessing crop health. By collecting unmanned aerial vehicle (UAV) hyperspectral images from 3 growth stages and combining them with ground-measured chlorophyll data, various machine learning and ensemble learning models were employed to estimate the chlorophyll content in spring wheat,and the estimation accuracy of different models were compared. The results showed that the canopy reflectance of spring wheat was generally consistent across different growth stages, but significant differences in spectral reflectance intensity were observed in the 770~900 nm wavelength range. 16 spectral indices all showed significant correlations with chlorophyll content, among which optimized vegetation index 1, plant biochemical index and normalized difference red edge index maintained high correlation throughout the entire growth cycle. The prediction accuracy of the Stacking and Voting ensemble learning models was higher than the basic models, with the Voting ensemble model performing particularly well. In the test set, determination coefficient (R2)values of 3 growth stages were 0.78, 0.77 and 0.73, and root mean square error (RMSE) values were 8.70, 11.36 and 16.17, respectively. Compared with random forest, support vector regression, K_nearest neighbor and ridge regression models, the R2 of the Voting model was on average 0.17, 0.14 and 0.22 higher, and the RMSE was 4.64, 2.54 and 6.51 lower, indicating its superior predictive ability. Above results provided new perspectives and methods for precision agriculture and crop health monitoring.

    Design and Experimentation of Conveying and Separating Device for Fresh-eating Sweet Potato Harvester in Sandy Soil
    Gang ZHENG, Peng XU, Dongquan CHEN, Songmei YANG, Minsheng WU, Ranbing YANG
    2025, 27(6):  104-112.  DOI: 10.13304/j.nykjdb.2024.0092
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    Aiming at the problems of mechanization harvesting high skin breaking rate of fresh-eating sweet potato under sandy soil, this study designed a rubber-wrapped integrated conveying and separating device. The device key components were designed, the quadratic regression orthogonal rotating combination design was adopted to conduct field tests and performance analysis with the line speed of the conveying and separating device, the amplitude of the jitter device, and the digging depth as test factors, and the sweet potato skin breaking rate and loss rate as the evaluation indexes, as well as the variance analysis response surface drawing and parameter optimization were carried out by using Design Expert software of the test results. Optimal working parameter combination after optimization: the line speed of the conveying and separating device was 0.28 m·s-1, the amplitude of the shaking device was 14 mm, the digging depth was 232 mm, forecast the skin breaking rate was 0.851%, the loss rate was 0.678%. According to the verification test get the parameter combination below the skin breaking rate was 0.866% and the loss rate was 0.694%, and the optimization results were basically consistent with the verification results, which met the requirements of fresh-eating sweet potato harvesting in sandy land.

    Research on End-to-end Low-light Environment Black Pig Detection Technology Integrating IAT Module
    Mengzhen HUANG, Hao LI, Huanjun HU, Zipeng LI, Zhongyin SHENG, Yifan LIU, Zhenyan XIA, Aoyun ZHENG
    2025, 27(6):  113-125.  DOI: 10.13304/j.nykjdb.2024.0493
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    To address the issues of poor image quality, difficulty in recognition and localization, as well as false positives and false negatives caused by occlusion and adhesion in scenarios involving clustered black pigs under low-light conditions, a detection model named low-light animal detection network (LADnet) was proposed. Firstly, an illumination-adaptive transformer (IAT) and a coordinate attention (CA) mechanism were utilized to enhance the brightness and reduce noise in the images. Then, a selective kernel convolutional attention (SKCA) module was designed to improve the model’s ability to perceive black pigs. Finally, the ReLU activation function was employed to mitigate problems related to gradient vanishing and explosion. The results showed that the LADnet model achieved precision, recall and mean average precision (mAP@0.5) of 97.32%, 86.61% and 92.73%, respectively, representing improvements of 1.07, 6.15 and 3.05 percentage points compared to the baseline model. Compared to single-stage object detection models such as SSD and YOLOv5, LADnet achieved an average accuracy improvement of 8.33 and 7.35 percentage points, respectively. In comparison with two-stage models like Cascade R-CNN, Faster R-CNN and DAB_DETR, LADnet not only demonstrated higher detection accuracy but also achieved a smaller parameter size and faster detection speed, making it more suitable for the real-time detection requirements. The LADnet model demonstrated exceptional detection performance and enhanced robustness in low-light black pig detection tasks, providing an efficient and reliable tool for the accurate identification of black pigs in low-light environments, which holded significant importance for advancing the development of intelligent farming under low-light condition.

    ANIMAL AND PLANT HEALTH
    Proteome Analysis of Rice Response to Gibberella fujikuroi Infection
    Lihua SHAO, Peng LI
    2025, 27(6):  126-135.  DOI: 10.13304/j.nykjdb.2024.0337
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    Bakanae disease, a common and highly harmful fungal disease of rice, seriously impacts rice yield. To clarify the up-regulated differential proteins of high-resistance varieties in response to the bakanae pathogen, the TMT (tandem mass tag) quantitative proteomics technology was employed to compare the proteomes before and after infection with the bakanae pathogen, and the significantly differentially expressed proteins with P≤0.05 and a fold change (FC)>1.2 or <0.83 were screened. The results showed that 124 up-regulated and 75 down-regulated differentially expressed proteins were screened. The 124 proteins with up-regulated expression were subjected to gene ontology (GO) enrichment analysis, and got 88 enrichment items, among which 4 items were significantly enriched, including 2 biological processes (response to stress and response to oxidative stress), 2 molecular functions (peroxidase activity and heme binding), which should be beneficial to remove peroxides in rice bud tissue and had a protective effect on cells. A total of 45 pathways were obtained by (kyoto encyclopedia of genes and genomes KEGG) enrichment analysis, and 3 pathways were significantly enriched for upregulated differential proteins including phenylpropanoid biosynthesis, the biosynthesis of secondary metabolites and phenylalanine metabolism, respectively, which should be beneficial toimprove disease resistance of rice. There were 3 differential proteins (Os01t0327400-01, Os12t0112000-01, Os01t0963000-04) were common among 4 items of GO significant enrichment and 3 pathways of KEGG significant enrichment, which could be the key proteins in the bakanae disease resistance. Above results provided scientific theoretical basis for the research of disease resistance.

    Preparation of Quercetin from Sophora japonica and Its Effect Together with Colistin on Salmonella spp.
    Xiaoyan XIONG, Dandan TIAN, Yuxin GONG, Yu QIAO, Xiaoqing XU, Mengxin HE, Bo SHI, Qing PENG
    2025, 27(6):  136-147.  DOI: 10.13304/j.nykjdb.2024.0157
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    Quercetin is a flavonoid compound with various biological activities, mostly existing in the form of glycosides in plants. Sophora japonica is an ideal raw material for preparing quercetin, with advantages such as wide planting area and low price. This study investigated the synergistic antibacterial effects of quercetin against Salmonella spp. that combined with colistin. The quercetin was prepared from the Sophora japonica buds Aspergillus niger fermentation and isolated from the semi-solid fermentation products using ultrasonic extraction methods, purified and structurally identified using HPLC, ESI-MS and NMR methods. The antibacterial activities of quercetin and colistin, both individually and in combination, against Salmonella were evaluated using dilution and checkerboard methods. The results showed that the highest product of quercetin was 73.7 mg·g-1 after fermentation for 96 h and the purity reached to 98% after purification and identification. After evaluation of antibacterial activity, the minimum inhibitory concentrations (MICs) of colistin against S. typhimuriumS. pullorum 1789 and 520 were 2, 8, and 4 μg·mL-1, respectively, while the MICs of quercetin against the mentioned strains were all above 500 μg·mL-1. When quercetin was used together with colistin, the synergistic antibacterial effects were observed against above Salmonella strains, particularly against S. pullorum 1789 and 520, with fractional inhibitory concentration index (FICI) values below 0.5, and the MIC value of colistin were reduced to 1 μg·mL-1. The study demonstrated the synergistic inhibitory effect of quercetin prepared from Sophora japonica through A. niger fermentation combined with colistin against Salmonella, providing a scientific basis for developing new antibacterial combination strategies.

    FOOD QUALITY & PROCESSING AND STORAGE
    Three-level Mid-infrared Spectroscopy Study of Tubers Starch Crystal/amorphous Structure and Thermostability
    Wei WANG, Linnan DU, Yuanyuan XU, Hongwei YU
    2025, 27(6):  148-157.  DOI: 10.13304/j.nykjdb.2023.0961
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    The structure of tubers starch including sweet potato starch, potato starch and tapioca starch were studied by one-dimensional mid-infrared spectroscopy (MIR) and second derivative MIR. The crystal/amorphous structure and thermostability of 3 kinds of starch (sweet patota starch, patota starch and tapioca starch) were also discovered by two dimensional MIR (2D-MIR). The results showed that the infrared absorption modes of tubers starch included C-O stretching vibration (νC-O-tubers). The tubers starch contained crystal and amorphous structures. With the increase of temperature (303~433 K), the main functional group of tubers starch crystal/amorphous structure showed different sensitivity and order to the heat. The amorphous structure of 3 kinds of starch were more sensitive to temperature changes, and changed firstly, while the crystal structure was relatively stable. Above results provided a theoretical basis for studying the properties of tubers starch and expanding the application of MIR spectroscopy in the food field.

    Effects of Gibberellic Acid on Berry and Raisin of Perlette Grape
    Shijian BAI, Jinge HU
    2025, 27(6):  158-169.  DOI: 10.13304/j.nykjdb.2024.0231
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    In order to investigate the effect of exogenous gibberellic acid (GA3) treatment on the quality of Perlette grapes and raisins, 5 treatments were set including no GA3 treatment during bloom(A) , inflorescence 75% blooming with 75 mg·L-1 GA3 treatment (B), inflorescence 100% blooming with 75 mg·L-1 GA3 treatment (C), 2 d post-bloom with 75 mg·L-1 GA3 treatment (D), and 4 d post-bloom with 75 mg·L-1 GA3 treatment (E). All the above treatments were treated with GA3 150 mg·L-1 at 10 d post-bloom, and no spraying GA3 during bloom and post-bloom was as the control (CK), berries quality, raisin quality and yield were assessed. The results showed that berry mass, cluster mass and berry hardness of Perlette grape was significantly increase by GA3 treatment, but the total soluble solids content was significantly decreased. The single raisin mass was significantly increased by GA3 treatment, phenolic content and antioxidant activity were enhanced, but plumpness was decreased, browning was decreased. The shape index of grapes and raisins were significantly increased by GA3 treatment during bloom, and promoted the color of grapes and raisins turn to green. The yield of raisins and the single mass of raisins were the highest in treatment D, with 12.56 t·hm-2, 1.30 g, respectively, and the raisin shape index was 2.42, relatively plump. The results of principal component analysis intuitively reflect the quality and yield distribution of grapes and raisins in each treatment. A comprehensive evaluation model was established, and it was found that treatment D had the highest comprehensive score. The best effect was treatment of 2 d post-bloom with 75 mg·L-1 GA3+10 d post-bloom with 150 mg·L-1 GA3. Above results provided technical reference for further optimizing and standardizing the use of GA3 in Perlette grapes, and improved the quality of grapes and raisins.

    BIO-MANUFACTURING & RESOURCE AND ECOLOGY
    Research Progress on Effect of Arbuscular Mycorrhizal Fungi on Soil Carbon Balance
    Ying ZHANG, Haihong GU, Yanjun AI, Fuping LI, Zijing WU
    2025, 27(6):  170-183.  DOI: 10.13304/j.nykjdb.2023-0971
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    Due to the increasing CO2 emissions from human activities, there is a pressing issue of global warming that poses a serious threat to the security of ecosystems. Arbuscular mycorrhizal fungi (AMF) have been able to form symbiotic systems with 80% of land plants, which have improved plant stress resistance and promoted plant growth, and have good application prospects. However, the effects of AMF on soil carbon storage have been unstable and the application technology have been immature, which have seriously limited their application.The impact of AMF on both the fixation and decomposition of soil carbon were systematically examined including how AMF influences plant photosynthesis, nutrient absorption and transformation within plants, microbial community activity near root systems, and soil carbon decomposition in proximity to roots. Furthermore, it addressed existing issues in practical applications with the aim of providing theoretical support for research on soil carbon balance through AMF.

    Characteristics of Runoff and Sediment Yield of Different Treatment Modes of Dump Slope in Typical Grassland Mining Area
    Pengli SHI, Yanping LIU, Jianying GUO, Zhenqi YANG, Jinsheng FAN
    2025, 27(6):  184-194.  DOI: 10.13304/j.nykjdb.2023.0906
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    In order to analysis the characteristics of runoff and sediment yield under different management modes of dump slope in typical grassland mining area, the Hesigewulanan open-pit coal mine in Xilinguole League was taken as the research object. 6 treatments were set up, including mixed grass (HC), mixed grass+shrub (HCG), mixed grass+diamond grid land preparation (HCS), mixed grass+pin-shaped land preparation (HCP), mixed grass+strip land preparation (HCH) and bare slope (CK). The K-means clustering method was used to divide the rainfall types in the study area. Combined with the runoff and sediment yield data of 15 natural rainfalls in runoff plots from 2021 to 2023, the characteristics of runoff and sediment yield under different management modes and their response to rainfall were analyzed. The results showed that, taking rainfall duration, rainfall and rainfall intensity as characteristic indexes, erosive rainfall could be divided into 3 types of rain, rain type Ⅰ showed short rainfall duration, small rainfall, heavy rainfall intensity, rain type Ⅱ showed medium rainfall duration, medium rainfall, medium rainfall intensity, rain type Ⅲ showed long rainfall duration, large rainfall, small rainfall intensity. There was no uniform rule for the cumulative runoff of various types of control measures with the change of rainfall type. Among them, HC had the least runoff in rain type I with 36.15 L, and HCS had the least runoff in rain type Ⅱ and Ⅲ with 36.14 and 23.15 L, respectively. Under single vegetation measure, the runoff and sediment yield of HC treatment were significantly less than those of HCG treatment, and under composite modes, the runoff and sediment yield of HCS treatment were significantly less than those of other treatments. In general, the multi-year cumulative runoff and sediment yield of various types of control measures showed HCS<HCH<HCP<HCG<HC<CK. The rainfall was the most significant index affecting runoff and sediment yield. The response characteristics of runoff and sediment yield in different treatments to various rainfall parameters were different. The HCS treatment had the best effect of runoff and sediment reduction and least affected by rainfall, while the runoff and sediment yield of CK were significantly affected by rainfall. Above results indicated that the layout of control measures could effectively prevent and control the soil and water loss of the dump slope. The layout of mixed grass+diamond grid land preparation measures had the best effect on reducing the runoff and sediment.

    Effects of Combined Application of Biochar and Nitrogen Fertilizer on Aggregate and Organic Carbon Content of Black Soil in Northeast China
    Fu QING, Hongyue LIANG, Jing SUN, Xinrui LU, Yunjiang LIANG
    2025, 27(6):  195-204.  DOI: 10.13304/j.nykjdb.2024.0007
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    In order to improve soil structure and enhance soil fertility in the black soil area, the long-term positioning experiment in the black soil area was used as the research platform to study the effects of the combination of biochar and nitrogen fertilizer on soil aggregate and organic carbon content, and to explore the maximum influencing factors. 3 biochar levels of 10 (C1), 20 (C2) and 50 g·kg-1 (C3) were set, and 3 nitrogen fertilizer levels of 0 (N0), 300 (N1) and 600 kg·hm-2 (N2) were set. The results showed, compared with CK, the soil bulk density decreased by 2.73%~8.20%, and the soil water content increased by 1.04%~31.24%, with no significant difference among all treatments. The combination application of biochar and nitrogen promoted the transformation of <0.053 mm aggregate to ≥2 mm aggregate. The aggregate stability was significantly improved, and the aggregate mass ratio (R0.25), mean weight diameter (MWD) and geometric mean diameter (GMD) of soil ≥0.25 mm increased by 25.06%, 21.71% and 12.50%, respectively. The organic carbon content of all grain grades of aggregates increased, and the organic carbon content of aggregates ≥2 mm and <0.053 mm increased significantly, with the increases of 13.19%~54.42% and 13.78%~41.34%, respectively. Principal component analysis (PCA) showed that R0.25 was the key factor affecting soil organic carbon content, and C2N1 treatment was the best application measure to improve the structure of black soil. Above research results provided a theoretical basis for curbing the degradation of black soil in northeast China and improving soil fertility.

    Effects of Different Foliar Fertilizers on Growth, Development and Fruit Quality of ‘Pinot Noir’ Grape
    Juanjuan HUANG, Zhiqiang ZHANG, Juan MAO, Zonghuan MA, Baihong CHEN
    2025, 27(6):  205-217.  DOI: 10.13304/j.nykjdb.2023.0890
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    To explore the effects of different foliar fertilizers on the growth, development and fruit quality of ‘Pinot Noir’ grapes, 5-year-old ‘Pinot Noir’ grapevines were used as the experimental materials. 6 types of foliar fertilizers, including inorganic nutrition type (T1), amino acid type (T2), hormone type (T3), water-soluble macronutrient type (T4), humic acid type (T5) and resistance induction type (T6) were sprayed, and clean water was sprayed as a control (CK). The changes of leaf area, new shoot base thickness, leaf SPAD value and mineral element content at different stages were analyzed, and the relevant indicators of fruit quality were determined at maturity stage. The results showed that the T5 treatment significantly increased the leaf area of grapes. At 15, 30, 45, 60 and 75 d after flowering, the leaf areas were increased by 14.10%, 12.34%, 15.47%, 15.27% and 15.70%, respectively, compared with that of CK. All treatments increased the new shoot base thickness of grape plants, with the T5 treatment showing the most significant effect. Compared with CK, each treatment increased the leaf SPAD values at different stages, but there was no significant difference between treatments. The mineral element content analysis showed that T1 treatment significantly increased the contents of macronutrients in leaves, among which K content was most significantly increased. T6 treatment had the best effect on the promotion of medium element content, while T5 treatment had a good effect on the accumulation of trace elements, and the accumulation of Cu was significantly increased at 60 and 75 d after flowering. In terms of fruit quality, T5 treatment significantly increased the anthocyanin, flavonoid, tannin and total phenolic content of grape fruits by 16.88%, 38.38%, 24.74% and 43.13%, respectively, compared with CK. Finally, principal component analysis of the fruit quality-related indicators at harvest showed that T5 treatment had the highest score. In conclusion, all 6 types of foliar fertilizers promoted the growth and fruit quality of ‘Pinot Noir’ grape, with T5 treatment, a humic acid type foliar fertilizer, showing the best results and significantly enhancing fruit quality. Above results provided theoretical guidance for improving grape quality and yield.

    Effects of Planting Years on Soil Salinization and Microbial Community in Facility Vineyards
    Xiaoyu QI, Yanjie GUO, Lu LIU, Zitao ZHANG, Lijuan ZHANG, Yanzhi JI
    2025, 27(6):  218-228.  DOI: 10.13304/j.nykjdb.2023.0953
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    In order to reveal the soil salinization characteristics of facility vineyards with different planting years and their effects on microbial communities, facility vineyards planted for 0 (farmland soil), 1~5, 6~10, 10~15 and >15 a were selected for investigation and sampling, and soil salt content, salt separators, microbial biomass carbon and nitrogen, and microbial community structure were analyzed. The results showed that the soil salt content of facility vineyard was significantly higher than that of farmland, and there was an obvious salinization trend. The increase of planting years aggravated the risk of soil salinization, and only the farmland soil was mildly saline, and the soil of the facility vineyard was moderately or severely saline, with a salt content of 3.47~5.12 g·kg-1. With the increase of planting years, the soluble cations and anions in each soil layer mainly increased with Na+ and SO42-, and the contents of Ca2+, Mg2+, K+, Na+, SO42- and Cl- in the surface soil showed a positive correlation with the salt content, and the correlation between Cl-,SO42- and the salt content reached a significant level. There was a positive correlation between soil microbial biomass carbon and nitrogen content and salt content, and the relationship between salt content and microbial biomass nitrogen was significant, and the microbial biomass carbon and nitrogen content was the lowest at facility vineyards planted for 6~10 a, which was 108.49 and 7.72 mg·kg-1, respectively, which was 55.66% and 80.97% lower than that of farmland soil. The relative abundance of Proteobacteria and Actinobacteria in soil bacterial communities of different planting years decreased with the increase of planting years, from 35.71% and 23.46% of 1~5 a to 31.60% and 11.61% when planting years >15 a. The relative abundance of firmicutes showed an upward trend with the increase of planting years. Cl-, Ca2+, K+ and Na+ were significant negatively correlated with the Shannon index of bacteria, and the correlation coefficient was between 0.89~0.98. In summary, with the increase of planting years, the degree of soil salinization increased, the soil bacterial diversity decreased, and the fungal diversity increased.

    Effect of Long-term Fertilization on Diversity of Bacterial Community and Distribution of ARGs in Grape Soil
    Huilai WANG, Shuai LI, Yin WANG, Dongtao WU, Jiawei MA, Zhengqian YE, Yongqing CHI, Mei WANG
    2025, 27(6):  229-239.  DOI: 10.13304/j.nykjdb.2024.0675
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    To examine the influence of long-term fertilization on the diversity of microbial community and the evolution of antibiotic resistance genes (ARGs) in grape soil, the grape soil continuously fertilized for 5 years was as material, with an adjacent plot of uncultivated and unfertilized soil as control (CK). Metagenomic sequencing technology and analysis of the physicochemical properties of the soil were employed to conduct a comprehensive investigation into the impact of long-term fertilization on the diversity of the microbial community and the distribution of ARGs in grape soil. The results revealed that, compared with CK, long-term fertilization resulted in a notable reduction of soil pH and a substantial alteration in soil nutrient composition. The contents of soil organic matter, total nitrogen and total potassium increased by 236.0%, 243.3% and 7.4%, respectively. Furthermore, the contents of alkali-soluble nitrogen and ammonium nitrogen increased significantly by 9.61 and 9.28 times, respectively. Additionally, the soil microbial biomass carbon and microbial biomass nitrogen increased by 19.2% and 8.53 times, respectively. Furthermore, compared with CK, long-term fertilization resulted in the addition of 218 new specific drug resistance genes and the reduction of 86 natural drug resistance genes in grape soil. The relative abundances of the genera Nitrospira and Anaeromyxobacter were decreased by 11.4% and 3.1%, respectively, as a consequence of the influence of physical and chemical factors such as soil pH, organic matter, total nitrogen and total potassium. The relative abundances of the genera PseudogracilibacillusStaphylococcusPseudolabrys and Virgibacillus increased by 10.5%, 6.1%, 0.6% and 5.4%, respectively. These changes in dominant bacterial groups were closely related to ARGs and played a role in regulating the overall function and metabolism of the grape soil microbiota. In conclusion, long-term fertilization could markedly enhance the physical and chemical properties of grape soil, while also should exert a profound influence on the diversity of soil microbial community and the number of ARGs.

    Effects of Different Water and Nitrogen Coupling on Physiological Indexes and Yield of Protein Mulberry
    Shichao CHEN, Ju WANG, Fuqiang GUO, Rui HAO, Jianping SHI
    2025, 27(6):  240-249.  DOI: 10.13304/j.nykjdb.2024.0096
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    In order to solve the problems of slow growth, low yield and low water and fertilizer utilization efficiency of protein mulberry in arid and semi-arid areas, the plant height, basal diameter, aboveground biomass and protein content of protein mulberry to different water and nitrogen treatments were analyzed by carrying out water and nitrogen coupling test of protein mulberry. At the same time, taking yield, water use efficiency and partial factor productivity of nitrogen fertilizer as the optimal objectives, the effects of different water and nitrogen coupling treatments on the yield, water and fertilizer use efficiency of protein mulberry were analyzed by regression analysis, and the regulation mode of promoting water and nitrogen was put forward. The results showed that the coupling of water and nitrogen had significant effect on soil moisture content in 20 to 40 cm soil layer. The interaction between nitrogen application rate and irrigation amount had significant effects on plant height and basal diameter of protein mulberry, and had extremely significant effects on aboveground biomass and leaf protein content. Regression analysis showed that the yield, water use efficiency and nitrogen partial factor productivity of protein mulberry were the best when the nitrogen application rate was 67.24~82.06 kg·hm-2 and the irrigation amount was 164.30~200.59 mm. Above results could provide theoretical support for the efficient planting of protein mulberry and the scientific management of water and fertilizer in arid and semi-arid areas, which was of great significance for improving the biological yield and benefit of protein mulberry.