水稻矮秆迟抽穗突变体d534的性状及其对赤霉素的敏感性分析

doi:10.13304/j.nykjdb.2023.0649

中国农业科技导报 ›› 2024, Vol. 26 ›› Issue (3): 7-14.DOI: 10.13304/j.nykjdb.2023.0649

水稻矮秆迟抽穗突变体d534的性状及其对赤霉素的敏感性分析

曾建光¹^,²(), 刘桃李¹^,², 孙林娟²^,³, 袁定阳²^,³, 黄钰博²^,³, 金晨钟¹(), 谭炎宁¹^,²^,³()

^1.湖南人文科技学院农业与生物技术学院，湖南娄底 417000
^2.国家耐盐碱水稻技术创新中心，海南三亚 572000
^3.海南大学热带作物学院，海口 570100

收稿日期:2023-08-29 接受日期:2023-11-22 出版日期:2024-03-15 发布日期:2024-03-07
通讯作者: 金晨钟,谭炎宁
作者简介:曾建光 E-mail：zjg2315763483@163.com
基金资助:
三亚崖州湾科技城管理局科技计划项目(202002006);海南省重大科技计划项目(ZDKJ2020001);湖南省自然科学基金资助项目(2021JJ30485)

Analysis of Character and GibberellinSensitivity of Rice Dwarfism and Late-Heading Mutant d534

Jianguang ZENG¹^,²(), Taoli LIU¹^,², Linjuan SUN²^,³, Dingyang YUAN²^,³, Yubo HUANG²^,³, Chenzhong JIN¹(), Yanning TAN¹^,²^,³()

^1.School of Agriculture and Biotechnology, Hunan University of Humanities and Technology, Hunan Loudi 417000, China
^2.National Saline-alkali Tolerant Rice Technology Innovation Center, Hainan Sanya 572000, China
^3.School of Tropical Crops, Hainan University, Haikou 570100, China

Received:2023-08-29 Accepted:2023-11-22 Online:2024-03-15 Published:2024-03-07
Contact: Chenzhong JIN,Yanning TAN

摘要/Abstract

摘要：

水稻半矮秆突变体在株型改良和高产育种中具有重要价值。前期通过辐射诱变籼稻品种五山丝苗（R534）获得了半矮秆且抽穗期延迟突变体d534，分析了d534的表型、遗传模式和GA₃敏感性。大田性状分析发现，和野生型R534相比，d534的抽穗期延迟了7 d，株高下降了29.22 cm，降幅为26.86%。d534的穗及倒1至倒5节都明显缩短，长度分别为野生型的71.27%、68.75%、70.18%、85.17%、77.86%和71.18%，说明d534的目的基因调控穗及茎节的伸长。遗传分析发现，d534的矮化和迟抽穗性状受同一核基因控制，呈隐性遗传。内源GA₃含量测定发现，一叶一心期d534茎中GA₃含量为0.17 ng·g^-1，比R534降低了60.47%，表明d534的矮化与内源GA₃合成不足有关。用25和50 mg·L^-1外源GA₃处理一叶一心期幼苗5 d，d534的株高比未处理组（0 mg·L^-1 GA₃）增加了71.76%和74.98%，且超过了未处理组R534的株高，说明外源GA₃能回补d534的株高表型；qRT-PCR分析发现，与R534相比，d534茎和叶中OsGA20ox2和OsGA3ox2基因表达水平均显著下调，表明d534内源GA₃合成不足可能与OsGA20ox2和OsGA3ox2基因表达水平下调有关。明确了d534是茎节缩短且内源GA₃积累不足的矮化突变体，为解析d534半矮秆性状形成的生物学机理奠定了基础。

关键词: 水稻, d534突变体, 半矮秆, 迟抽穗, 赤霉素

Abstract:

Rice semi-dwarf mutants play important roles in plant type improvement and high-yield breeding. Previously， a semi-dwarf mutant named d534 was obtained by mutagenesis from the indica rice cv. Wushan Simiao （R534）. The phenotype， genetic module and GA₃ sensitivity of d534 were analyzed in this study. Field observations found that， compared with the wild type R534， d534 delayed to head for 7 d and plant height decreased 29.22 cm（by 26.86%）. The panicle and the first internode from top 1^st to 5^th internode were significantly shortened by 71.27%， 68.75%， 70.18%， 85.17%， 77.86% and 71.18% compared with wild type in the length， indicating that the target gene of d534 would control the elongation of panicle and all internodes. Genetic analysis revealed that the phenotypes of dwarfism and late-heading were both controlled by the same recessive nuclear gene. The determination of endogenous GA₃ content showed that the GA₃ in the stem of d534 seedlings was 0.17 ng·g^-1， which was 60.47% lower than that of R534， indicating that the phenotype of dwarfism in d534 was related to the lack of endogenous GA₃. After treated with 25 and 50 mg·L^-1 GA₃ for 5 d on d534 seedlings， the plant height was 71.76% and 74.98% higher than that of untreated group （0 mg·L^-1 GA₃）. Meanwhile， such a height was more than that of untreated R534 seedlings， indicating that exogenous GA₃ would rescue the plant height of d534. qRT-PCR analysis showed that the expression levels of OsGA20ox2 and OsGA3ox2 genes in d534 stem and leaf were significantly down-regulated compared with R534， indicating that the insufficient synthesis of endogenous GA₃ in d534 might be related to the down-regulation of OsGA20ox2 and OsGA3ox2 gene expression levels. This study confirmed that d534 was a semi-dwarf mutant with shortened internodes and insufficient endogenous GA₃. It provided basis for revealing the biological mechanism underlying the semi-dwarfism in d534.

Key words: rice, d534 mutant, semi-dwarfism, late-heading, gibberellin

中图分类号:

S511

曾建光, 刘桃李, 孙林娟, 袁定阳, 黄钰博, 金晨钟, 谭炎宁. 水稻矮秆迟抽穗突变体d534的性状及其对赤霉素的敏感性分析[J]. 中国农业科技导报, 2024, 26(3): 7-14.

Jianguang ZENG, Taoli LIU, Linjuan SUN, Dingyang YUAN, Yubo HUANG, Chenzhong JIN, Yanning TAN. Analysis of Character and GibberellinSensitivity of Rice Dwarfism and Late-Heading Mutant d534[J]. Journal of Agricultural Science and Technology, 2024, 26(3): 7-14.

图/表 7

参考文献 29

1	JENNINGS P R. Plant type as a rice breeding objective [J]. Crop Sci., 1964, 4(1):13-15.
2	余汉勇,魏兴华,王一平,等.等位酶和SSR标记研究水稻矮仔占衍生品种的遗传差异[J].中国水稻科学, 2004(6):3-8.
	YU H Y, WEI X H, WANG Y P, et al.. Morphological, allozyme and SSR markers were used to study the genetic diversity of rice dwarf accounted derivatives [J]. Chin. J. Rice Sci., 2004(6):3-8.
3	PENG S, CASSMAN K G, VIRMANI S S, et al.. Yield potential trends of tropical rice since the release of IR8 and the challenge of increasing rice yield potential [J]. Crop Sci., 1999, 39(6):1552-1559.
4	LANGE T, PIMENTA LANGE M J. The multifunctional dioxygenases of gibberellin synthesis [J]. Plant Cell Physiol., 2020, 61 (11):1869-1879.
5	ITOH H, TATSUMI T, SAKAMOTO T, et al.. A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase [J]. Plant Mol. Biol., 2004, 54(4):533-547.
6	SPIELMEYER W, ELLIS M H, CHANDLER P M, et al.. Semidwarf (sd-1), ‘green revolution’ rice, contains a defective gibberellin 20-oxidase gene [J]. Proc. Natl. Acad. Sci. USA, 2002, 99(13):9043-9048.
7	SAKAMOTO T, MORINAKA Y, ISHIYAMA K, et al.. Genetic manipulation of gibberellin metabolism in transgenic rice [J]. Nat. Biotechnol., 2003, 21(8):909-913.
8	SUN T P. The molecular mechanism and evolution of the GA-GID1-DELLA signaling module in plants [J]. Curr. Biol., 2011, 21(9):R338-R345.
9	YOSHIDA H, TANIMOTO E, HIRAI T, et al.. Evolution and diversification of the plant gibberellin receptor GID1 [J]. Proc. Natl. Acad. Sci. USA, 2018, 115 (33):E7844-E7853.
10	GOMI K, SASAKI A, ITOH H, et al.. GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice [J]. Plant J., 2004, 37(4):626-634.
11	FANG Z, JI Y, HU J, et al.. Strigolactones and brassinosteroids antagonistically regulate the stability of the D53-OsBZR1 complex to determine FC1 expression in rice tillering [J]. Mol. Plant, 2020, 13(4):586-597.
12	WEN X, SUN L, CHEN Y, et al.. Rice dwarf and low tillering 10 (OsDLT10) regulates tiller number by monitoring auxin homeostasis [J/OL]. Plant Sci., 2020, 297:110502 [2023-11-29]. .
13	CHEN D, QIU Z, HE L, et al.. The rice LRR-like 1 protein YELLOW AND PREMATURE DWARF 1 is involved in leaf senescence induced by high light [J]. J. Exp. Bot., 2021, 72(5):1589-1605.
14	黄道强,周少川,李宏.优质稻新品种五山丝苗的选育及利用[J].广东农业科学, 2011, 38(9):15-16.
	HUANG D Q, ZHOU S C, LI H. Breeding and utilization of new high quality rice variety wushan silk seedling [J]. Guangdong Agric. Sci., 2011, 38(9):15-16.
15	谭炎宁,杨震,袁定阳.γ 射线诱变籼稻保持系 T98B 突变体的鉴定与农艺性状分析[J].激光生物学报, 2019, 28(3):274-280.
	TANG Y N, YANG Z, YUAN D Y. Identification and agronomic traits analysis of γ-ray mutagenesis of maintainer line T98B in indica rice [J]. Acta Laser Biol. Sin., 2019, 28(3):274-280.
16	CHEN Z, LIN T, WANG K. A powerful variant-set association test bbased on chi-square distribution [J]. Genetics, 2017, 207(3):903-910.
17	MULLER M, MUNNE-BOSCH S. Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry [J]. Plant Methods, 2011, 7:37.
18	KENNETH J, LIVAK, THOMAS D, et al.. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method [J]. Methods, 2001, 25 (4):402-408.
19	袁隆平.选育超高产杂交水稻的进一步设想[J].杂交水稻, 2012, 27(6):1-2.
	YUAN L P. Further ideas on breeding super high yield hybrid rice [J]. Hybrid Rice, 2012, 27(6):1-2.
20	谷福林,翟虎渠,万建民,等.水稻矮秆性状研究及矮源育种利用[J].江苏农业学报, 2003(1):48-54.
	GU F L, DI H Q, WAN J M, et al.. Studies on rice dwarf traits and utilization of dwarf source breeding [J]. Jiangsu J. Agric. Sci., 2003(1):48-54.
21	FERRERO-SERRANO Á, CANTOS C, ASSMANN S M. The role of dwarfing traits in historical and modern agriculture with a focus on rice [J/OL]. Cold Spring Harb Perspect Biol., 2019, 11(11):a034645 [2023-11-29].
22	NIU Y, CHEN T, ZHAO C, et al.. Improving crop lodging resistance by adjusting plant height and stem strength [J/OL]. Agronomy, 2021, 11(12):2421 [2023-11-29]. .
23	ITOH H, UEGUCHI-TANAKA M, SATO Y, et al.. The gibberellin signaling pathway is regulated by the appearance and disappearance of SLENDER RICE1 in nuclei [J]. Plant Cell, 2002, 14(1):57-70.
24	BISWAS S, TIAN J, LI R, et al.. Investigation of CRISPR/Cas9-induced SD1 rice mutants highlights the importance of molecular characterization in plant molecular breeding [J]. J. Genet. Genom., 2020, 47(5):273-280.
25	AOKI T, KITANO H, KAMEYA N, et al.. Accelerated shoot overgrowth of rice mutant ao-1 is epistatic to gibberellin-sensitive and -insensitive dwarf mutants [J]. J. Plant Res., 2002, 115(3):195-202.
26	ANGIRA B, ADDISON CK, CERIOLI T, et al.. Haplotype characterization of the sd1 semidwarf gene in united states rice [J]. Plant Genome, 2019, 12 (3):1-9.
27	ITOH H, UEGUCHI-TANAKA M, SENTOKU N, et al.. Cloning and functional analysis of gibberellin 3β-hydroxylase genes that are differently expressed during the growth of rice [J]. Proc. Natl. Acad. Sci. USA, 2001(98):8909-8914.
28	XUE Y, ZHANG Y, SHAN J, et al.. Growth repressor gmRAV binds to the gmGA3ox promoter to negatively regulate plant height development in soybean [J/OL]. Int. J. Mol. Sci., 2022, 23(3):1721 [2023-11-29]. .
29	WANG R, YANG X, GUO S, et al.. MiR319-targeted OsTCP21 and OsGAmyb regulate tillering and grain yield in rice [J]. J. Integr. Plant Biol., 2021, 63(7):1260-1272.

性状Character		R534	d534
播种-抽穗历期 Sowing-to-heading time/d		85	91
单株有效穗 Effective panicle per plant		7.63±1.37	8.25±1.75^*
株高 Plant height/cm		108.80±4.71	79.58±2.73^**
穗长 Panicle length/cm		24.64±4.94	17.56±2.06^**
倒1节 1^st internode from the top	长度Length/cm	34.80±1.92	23.43±3.73^**
倒1节 1^st internode from the top	直径Diameter/cm	0.38±0.04	0.46±0.03^**
倒2节 2^nd internode from the top	长度Length/cm	20.32±3.62	14.26±3.94^**
倒2节 2^nd internode from the top	直径Diameter/cm	0.46±0.02	0.58±0.03^**
倒3节 3^rd internode from the top	长度Length/cm	15.57±4.77	11.07±2.23^**
倒3节 3^rd internode from the top	直径Diameter/cm	0.51±0.05	0.68±0.03^**
倒4节 4^th internode from the top	长度Length/cm	7.27±2.63	5.56±1.24^**
倒4节 4^th internode from the top	直径Diameter/cm	0.62±0.02	0.79±0.03^**
倒5节 5^th internode from the top	长度Length/cm	3.92±0.48	2.79±0.31^**
倒5节 5^th internode from the top	直径Diameter/cm	0.66±0.04	0.88±0.05^**

性状Character		R534	d534
播种-抽穗历期 Sowing-to-heading time/d		85	91
单株有效穗 Effective panicle per plant		7.63±1.37	8.25±1.75^*
株高 Plant height/cm		108.80±4.71	79.58±2.73^**
穗长 Panicle length/cm		24.64±4.94	17.56±2.06^**
倒1节 1^st internode from the top	长度Length/cm	34.80±1.92	23.43±3.73^**
倒1节 1^st internode from the top	直径Diameter/cm	0.38±0.04	0.46±0.03^**
倒2节 2^nd internode from the top	长度Length/cm	20.32±3.62	14.26±3.94^**
倒2节 2^nd internode from the top	直径Diameter/cm	0.46±0.02	0.58±0.03^**
倒3节 3^rd internode from the top	长度Length/cm	15.57±4.77	11.07±2.23^**
倒3节 3^rd internode from the top	直径Diameter/cm	0.51±0.05	0.68±0.03^**
倒4节 4^th internode from the top	长度Length/cm	7.27±2.63	5.56±1.24^**
倒4节 4^th internode from the top	直径Diameter/cm	0.62±0.02	0.79±0.03^**
倒5节 5^th internode from the top	长度Length/cm	3.92±0.48	2.79±0.31^**
倒5节 5^th internode from the top	直径Diameter/cm	0.66±0.04	0.88±0.05^**

杂交组合 Hybrid combinations	世代 Generation	群体单株数量 Number of individuals	矮化晚花 Dwarfing and late flowering	正常株高正常花期 Normal plant height and flowering period	实际分离比 Actual separation ratio	χ²（χ²_0.05≤3.84）
d534/R534	F₁	35	0	35	—	—
R534/d534	F₁	26	0	26	—	—
d534/R534	F₂	1 331	342	989	1∶2.89	0.018
R534/d534	F₂	980	256	724	1∶2.83	0.029

杂交组合 Hybrid combinations	世代 Generation	群体单株数量 Number of individuals	矮化晚花 Dwarfing and late flowering	正常株高正常花期 Normal plant height and flowering period	实际分离比 Actual separation ratio	χ²（χ²_0.05≤3.84）
d534/R534	F₁	35	0	35	—	—
R534/d534	F₁	26	0	26	—	—
d534/R534	F₂	1 331	342	989	1∶2.89	0.018
R534/d534	F₂	980	256	724	1∶2.83	0.029

材料 Material	GA₃质量浓度 GA₃ content/（mg·L^-1）	株高 Plant height/cm
材料 Material	GA₃质量浓度 GA₃ content/（mg·L^-1）	0 d	5 d	10 d
R534	0	14.21±0.51	19.72±0.87	27.34±1.94
	25	14.09±0.44	28.56±1.85^**	44.23±2.87^**
	50	14.11±0.38	29.13±2.01^**	45.16±2.54^**
d534	0	11.27±0.59	16.29±1.08	21.35±1.85
	25	11.32±0.52	23.51±1.95^**	36.67±2.82^**
	50	11.37±0.61	23.88±2.15^**	37.36±3.94^**

水稻矮秆迟抽穗突变体d534的性状及其对赤霉素的敏感性分析

Analysis of Character and GibberellinSensitivity of Rice Dwarfism and Late-Heading Mutant d534

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