紫外-可见光谱库在农药杀菌剂隐性排查中的应用

doi:10.13304/j.nykjdb.2023.0247

摘要/Abstract

摘要：

为建立紫外-可见光谱库以期能够快速准确的检索农药杀菌剂中添加的隐性成分，通过高效液相色谱仪，以乙腈和水做为流动相，采用梯度洗脱分离及提取98种常规杀菌剂标准物质的紫外-可见光谱图，以标准物质的紫外-可见光谱图来建立紫外-可见光谱库。根据光谱的相似度和标样的保留时间来匹配目标化合物，从而检索出农药制剂中添加的隐性成分。通过2个杀菌剂来验证谱库检索的准确性，结果显示，谱库检索的正确性达100%。该方法具有检测目标化合物准确率高、操作简单、易学、成本较低等优点。

关键词: 高效液相色谱仪, 杀菌剂, 紫外-可见光谱库, 农药

Abstract:

To establish the ultraviolet-visible （UV-VIS） spectral library to quickly and accurately retrieve the hidden components added in the pesticide fungicide， with acetonitrile and water as the mobile phase， the UV-VIS spectrograms of 98 conventional bactericide standards were separated and extracted by gradient elution using a high-performance liquid chromatograph， and the UV-VIS spectrograms of the standard substances were used to establish the UV-VIS spectrogram library. The target compound was matched according to the spectral similarity and the retention time of the standard sample， so as to retrieve the hidden components added in the pesticide preparation. 2 bactericides were used to verify the accuracy of the spectral library retrieval， and the results showed that the accuracy of the spectral library retrieval reached 100%. This method had the advantages of high accuracy， easy operation and low cost.

Key words: high-performance liquid chromatography, bactericide, ultraviolet-visible spectrum library, pesticide

中图分类号:

陈颖, 刘小锋, 陈一萍, 廖丽萍, 傅洪涛. 紫外-可见光谱库在农药杀菌剂隐性排查中的应用[J]. 中国农业科技导报, 2024, 26(12): 122-128.

Ying CHEN, Xiaofeng LIU, Yiping CHEN, Liping LIAO, Hongtao FU. Application of UV-visible Spectral Library in Recessive Screening of Pesticide Fungicides[J]. Journal of Agricultural Science and Technology, 2024, 26(12): 122-128.

图/表 15

表1 梯度洗脱

Table 1 Gradient elution

时间 Time/min	单元 Unit	处理命令 Processing command	值 Value
0.01	泵 Pump	BConc	30
8.00	泵 Pump	BConc	30
8.01	泵 Pump	BConc	72
18.00	泵 Pump	BConc	72
18.01	泵 Pump	BConc	92
27.00	泵 Pump	BConc	92
27.01	泵 Pump	BConc	30
34.00	泵 Pump	BConc	30
34.01	控制器 Controller	停止 Stop	—

图1 10%苯醚甲环唑悬浮剂的液相色谱图

Fig. 1 Liquid chromatogram of 10% difenoconazole deflocculant

图2 A1峰化合物的紫外-可见光谱库检索图

Fig. 2 Search of peak A1 in UV-VIS spectral library

表2 A1峰化合物的紫外-可见光谱库检索结果

Table 2 Retrieval result of peak A1 in UV-VIS spectral library

峰 Peak	A1峰 Peak A1
序列号 Serial number	13
相似度 Similarity	0.999 971
化合物名称 Name of compound	嘧菌酯标 Azoxystrobin
保留时间 Retention time/min	16.237

图3 A2峰化合物的紫外-可见光谱库检索图

Fig. 3 Search of peak A2 in UV-VIS spectral library

表3 A2峰化合物的紫外-可见光谱库检索结果

Table 3 Retrieval result of peak A2 in UV-VIS spectral library

峰 Peak	A2峰 Peak A2
序列号 Serial number	585
相似度 Similarity	0.999 917
化合物名称 Name of compound	苯醚甲环唑标 Difenoconazole
保留时间 Retention time/min	19.584

图4 A3峰化合物的紫外-可见光谱库检索图

Fig. 4 Search of peak A3 in UV-VIS spectral library

表4 A3峰化合物的紫外-可见光谱库检索结果

Table 4 Retrieval result of peak A3 in UV-VIS spectral library

峰 Peak	A3峰 Peak A3
序列号 Serial number	669
相似度 Similarity	0.999 451
化合物名称 Name of compound	吡唑醚菌酯标 Pyraclostrobin
保留时间 Retention time/min	20.911

图5 40%戊唑醇多菌灵悬浮剂的液相色谱图

Fig. 5 Liquid chromatogram of 40% pentazole alcohol and carbendazim deflocculant

图6 B1峰化合物的紫外-可见光谱库检索图

Fig. 6 Search of peak B1 in UV-VIS spectral library

表5 B1峰化合物的紫外-可见光谱库检索结果

Table 5 Retrieval result of peak B1 in UV-VIS spectral library

峰 Peak	B1峰 Peak B1
序列号 Serial number	18
相似度 Similarity	0.999 297
化合物名称 Name of compound	多菌灵标 Carbendazim
保留时间 Retention time/min	7.302

图7 B2峰化合物的紫外-可见光谱库检索图

Fig. 7 Search of peak B2 in UV-VIS spectral library

表6 B2峰化合物的紫外-可见光谱库检索结果

Table 6 Retrieval result of peak B2 in UV-VIS spectral library

峰 Peak	B2峰 Peak B2
序列号 Serial number	444
相似度 Similarity	0.999 840
化合物名称 Name of compound	福美双标 Thiram
保留时间 Retention time/min	14.337

图8 B3峰化合物的紫外-可见光谱库检索图

Fig. 8 Search of peak B3 in UV-VIS spectral library

表7 B3峰化合物的紫外-可见光谱库检索结果

Table 7 Retrieval result of peak B3 in UV-VIS spectral library

峰 Peak	B3峰 Peak B3
序列号 Serial number	184
相似度 Similarity	0.999 596
化合物名称 Name of compound	戊唑醇标 Tebuconazole
保留时间 Retention time/min	17.007

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