›› 2013, Vol. 15 ›› Issue (5): 1-7.DOI: doi:10.3969/j.issn.10080864.2013.05.01

• 农业微生物制剂与酶产品创制特稿 •    下一篇

嗜酸糖苷水解酶研究进展

罗会颖1,姚斌1*,范云六2   

  1. (1.中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081; 2.中国农业科学院生物技术研究所| 北京 100081)
  • 出版日期:2013-10-15 发布日期:2013-12-16
  • 通讯作者: 姚斌,研究员,博士,博士生导师,主要从事饲料酶研究。E\|mail:binyao@caas.cn
  • 作者简介:罗会颖|副研究员|博士|主要从事极端酶及酶工程研究。E\|mail:luohuiying@caas.cn。
  • 基金资助:

    国家杰出青年科学基金(31225026);国家863计划项目(2012AA022208)资助。

Research Progress on Acidophilic Glycoside Hydrolase

LUO Hui\|ying1, YAO Bin1*, FAN Yun\|liu2   

  1. (1.Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081|2.Biotechnology Research Institute, CAAS, Beijing 100081, China)
  • Online:2013-10-15 Published:2013-12-16

摘要:

随着极端微生物及极端酶的广泛研究,嗜酸酶因其在极端酸性环境中具有高的酶活性和稳定性而倍受关注,并取得了较大的研究进展。嗜酸糖苷水解酶是嗜酸酶中最重要的一类,在生物能源、饲料、食品等工业中具有重要的应用前景。综述了重要嗜酸糖苷水解酶,包括嗜酸淀粉酶、嗜酸纤维素酶、嗜酸木聚糖酶和甘露聚糖酶在基因的挖掘、表达、分子改良嗜酸机制研究以及应用等方面国内外的研究进展,展望了嗜酸糖苷水解酶未来可能的研究方向和发展前景。

关键词: 极端酶;嗜酸菌;嗜酸糖苷水解酶;嗜酸机制

Abstract:

Extremophiles and enzymes from extremophiles are widely studied. Of them, acidophilic enzyme attracts much attention, due to its high activity and stability under extreme acidic conditions, and this research has made rapid progress. Acidophilic glycosyl hydrolase is one of the most important acidophilic enzymes, and has significant application prospect in bio\|energy, animal feed, food and other industries. This paper reviewed the gene cloning, heterologous expression, molecular modification and acidophilic mechanisms of important acidophilic glycosyl hydrolases, including amylase, cellulase, xylanase, and mannanase. The research orientation and development prospects were also elucidated in this paper.

Key words: extremozyme, acidophilic microbe, acidophilic glycoside hydrolase, acidophilic mechanism

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