中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (4): 154-161.DOI: 10.13304/j.nykjdb.2021.0128
彭田伟1(), 谢会雅2, 李思军3, 刘怡轩2, 帅开峰1,4, 彭媛媛1, 王青1, 李迪秦1(
)
收稿日期:
2021-02-03
接受日期:
2021-04-14
出版日期:
2022-04-15
发布日期:
2022-04-19
通讯作者:
李迪秦
作者简介:
彭田伟 E-mail: 1394111457@qq.com;
基金资助:
Tianwei PENG1(), Huiya XIE2, Sijun LI3, Yixuan LIU2, Kaifeng SHUAI1,4, Yuanyuan PENG1, Qing WANG1, Diqin LI1(
)
Received:
2021-02-03
Accepted:
2021-04-14
Online:
2022-04-15
Published:
2022-04-19
Contact:
Diqin LI
摘要:
为探讨不同浓度的植物生长调节剂和微生物菌剂复配施用对烟苗素质的影响,以烤烟新品系20619为材料,采用漂浮育苗,在烟苗4叶1心期喷施不同浓度的复硝酚钠和枯草芽孢杆菌菌剂复配剂,对8叶1心(成苗期)烟苗主要生长和生理指标进行测定与分析。结果表明,复硝酚钠和枯草芽孢杆菌菌剂复配喷施后,8叶1心烟苗的干物质积累、根系活力、叶片叶绿素含量及硝酸还原酶活性,均好于对照;且以喷施20 mg的1.8%复硝酚钠和7.5 g的枯草芽孢杆菌菌剂处理,烟苗全株干重(4.98 g·株-1)、干鲜比、根系活力(93.01 μg·g-1·h-1)、叶片叶绿素含量(0.548 5 mg·g-1)及硝酸还原酶(1.434 U·g-1)活性均表现最高;叶片中的过氧化氢酶、超氧化物岐化酶和过氧化物酶活性以及丙二醛含量均表现为最高。表明对4叶1心烟苗喷施复硝酚钠和枯草芽孢杆菌菌剂复配剂,有利于提高烟苗素质和培育壮苗。
中图分类号:
彭田伟, 谢会雅, 李思军, 刘怡轩, 帅开峰, 彭媛媛, 王青, 李迪秦. 复硝酚钠和枯草芽孢杆菌复配对烟苗生长和生理指标的影响[J]. 中国农业科技导报, 2022, 24(4): 154-161.
Tianwei PENG, Huiya XIE, Sijun LI, Yixuan LIU, Kaifeng SHUAI, Yuanyuan PENG, Qing WANG, Diqin LI. Effects of Sodium Dinitrate with Bacillus Sbutilis Complex on Growth and Physiological Indexes of Tobacco Seedlings[J]. Journal of Agricultural Science and Technology, 2022, 24(4): 154-161.
处理 Treatments | 复硝酚钠 Sodium dinitrophenol | 枯草芽孢杆菌菌剂 Bacillus subtilis agent | ||
---|---|---|---|---|
施用量 Applied content/mg | 含量 Content/% | 施用量 Applied content/g | 含量 Content/% | |
T1 | 20.0 | 0.398 | 7.5 | 0.148 |
T2 | 20.0 | 0.398 | 0.0 | 0.000 |
T3 | 15.0 | 0.299 | 7.5 | 0.148 |
T4 | 15.0 | 0.299 | 0.0 | 0.000 |
T5 | 10.0 | 0.199 | 7.5 | 0.148 |
T6 | 10.0 | 0.199 | 0.0 | 0.000 |
CK | 0.0 | 0.000 | 0.0 | 0.000 |
表1 不同处理施用量
Table 1 Applied content of different treatments
处理 Treatments | 复硝酚钠 Sodium dinitrophenol | 枯草芽孢杆菌菌剂 Bacillus subtilis agent | ||
---|---|---|---|---|
施用量 Applied content/mg | 含量 Content/% | 施用量 Applied content/g | 含量 Content/% | |
T1 | 20.0 | 0.398 | 7.5 | 0.148 |
T2 | 20.0 | 0.398 | 0.0 | 0.000 |
T3 | 15.0 | 0.299 | 7.5 | 0.148 |
T4 | 15.0 | 0.299 | 0.0 | 0.000 |
T5 | 10.0 | 0.199 | 7.5 | 0.148 |
T6 | 10.0 | 0.199 | 0.0 | 0.000 |
CK | 0.0 | 0.000 | 0.0 | 0.000 |
图1 不同处理下烟苗植株鲜重注:同一部位不同小写字母表示差异显著(P<0.05)。
Fig.1 Fresh weight of tobacco seedings under different treatmentsNote: Different small letters at the same part indicate significant difference(P<0.05).
图2 不同处理下烟苗植株干重注:同一部位不同小写字母表示差异显著(P<0.05)。
Fig.2 Dry weight of tobacco seedings under different treatmentsNote: Different small letters at the same part indicate significant difference(P<0.05).
图3 不同处理烟苗植株鲜干比及全株干重根冠比注:同一部位不同小写字母表示差异显著(P<0.05)。
Fig.3 Radio of tobacco seedings fresh and dry,root?shoot ratio of dry weight of whole plant with different treatmentsNote: Different small letters at the same part indicate significant difference(P<0.05).
处理 Treatment | 根系活力 Roots vigor/(µg·g-1·h-1) |
---|---|
T1 | 93.01±0.24 a |
T2 | 72.07±0.92 d |
T3 | 84.99±1.78 b |
T4 | 71.66±0.43 d |
T5 | 78.56±0.66 c |
T6 | 68.37±0.39 e |
CK | 63.87±9.64 f |
表2 不同处理烟苗根系活力
Table 2 Tobacco seeding roots vigor of different treatments
处理 Treatment | 根系活力 Roots vigor/(µg·g-1·h-1) |
---|---|
T1 | 93.01±0.24 a |
T2 | 72.07±0.92 d |
T3 | 84.99±1.78 b |
T4 | 71.66±0.43 d |
T5 | 78.56±0.66 c |
T6 | 68.37±0.39 e |
CK | 63.87±9.64 f |
处理 Treatment | 叶绿素a含量 Chlorophyll a content/ (mg·g-1) | 叶绿素b含量 Chlorophyll b content/ (mg·g-1) | 叶绿素(a+b)含量 Chlorophyll (a+b) content/ (mg·g-1) |
---|---|---|---|
T1 | 0.368 0±0.002 9 a | 0.180 5±0.003 9 a | 0.548 4±0.003 4 a |
T2 | 0.312 8±0.002 6 c | 0.126 7±0.001 4 c | 0.439 5±0.004 0 c |
T3 | 0.348 6±0.003 9 b | 0.146 1±0.001 7 b | 0.494 7±0.005 1 b |
T4 | 0.297 6±0.000 9 d | 0.116 6±0.002 6 d | 0.414 2±0.003 5 d |
T5 | 0.333 3±0.005 1 c | 0.125 1±0.002 9 c | 0.458 4±0.005 4 c |
T6 | 0.271 6±0.001 7 e | 0.089 1±0.002 2 e | 0.360 7±0.002 9 e |
CK | 0.222 7±0.023 1 f | 0.073 7±0.001 0 f | 0.296 4±0.022 7 f |
表3 不同处理烟苗叶片叶绿素含量
Table 3 Chlorophyll content under of tobacco seeding leaves different treatments
处理 Treatment | 叶绿素a含量 Chlorophyll a content/ (mg·g-1) | 叶绿素b含量 Chlorophyll b content/ (mg·g-1) | 叶绿素(a+b)含量 Chlorophyll (a+b) content/ (mg·g-1) |
---|---|---|---|
T1 | 0.368 0±0.002 9 a | 0.180 5±0.003 9 a | 0.548 4±0.003 4 a |
T2 | 0.312 8±0.002 6 c | 0.126 7±0.001 4 c | 0.439 5±0.004 0 c |
T3 | 0.348 6±0.003 9 b | 0.146 1±0.001 7 b | 0.494 7±0.005 1 b |
T4 | 0.297 6±0.000 9 d | 0.116 6±0.002 6 d | 0.414 2±0.003 5 d |
T5 | 0.333 3±0.005 1 c | 0.125 1±0.002 9 c | 0.458 4±0.005 4 c |
T6 | 0.271 6±0.001 7 e | 0.089 1±0.002 2 e | 0.360 7±0.002 9 e |
CK | 0.222 7±0.023 1 f | 0.073 7±0.001 0 f | 0.296 4±0.022 7 f |
处理 Treatment | 超氧化物岐化酶SOD/(U·g-1) | 过氧化物酶 POD/(U·g-1) | 过氧化氢酶 CAT/(U·mg-1) | 硝酸还原酶 NR/(U·g-1) | 丙二醛 MDA/(U·mg-1) |
---|---|---|---|---|---|
T1 | 88.92±1.39 a | 109.5±31.1 a | 44.36±0.42 a | 1.434±0.304 a | 16.94±0.07 a |
T2 | 74.37±0.36 c | 60.3±16.3 b | 14.89±0.20 d | 0.591±0.019 c | 12.90±0.02 d |
T3 | 87.84±0.72 a | 89.8±14.7 ab | 39.88±0.30 b | 0.917±0.003 b | 14.47±0.08 b |
T4 | 66.81±1.15 d | 68.9±8.0 b | 14.51±0.15 d | 0.520±0.008 c | 12.04±0.04 e |
T5 | 85.12±1.92 b | 86.0±17.6 ab | 34.52±0.27 c | 0.812±0.006 b | 13.78±0.10 c |
T6 | 63.68±1.54 e | 64.8±10.8 b | 13.68±0.20 d | 0.501±0.005 c | 11.06±0.02 f |
CK | 59.02±1.05 f | 58.4±4.8 b | 9.95±0.09 f | 0.451±0.003 c | 8.97±0.04 g |
表4 不同配施处理下烟苗叶片酶活性及丙二醛含量
Table 4 Enzyme activity and malondialdehyde content of tobacco seeding leaves under different treatments
处理 Treatment | 超氧化物岐化酶SOD/(U·g-1) | 过氧化物酶 POD/(U·g-1) | 过氧化氢酶 CAT/(U·mg-1) | 硝酸还原酶 NR/(U·g-1) | 丙二醛 MDA/(U·mg-1) |
---|---|---|---|---|---|
T1 | 88.92±1.39 a | 109.5±31.1 a | 44.36±0.42 a | 1.434±0.304 a | 16.94±0.07 a |
T2 | 74.37±0.36 c | 60.3±16.3 b | 14.89±0.20 d | 0.591±0.019 c | 12.90±0.02 d |
T3 | 87.84±0.72 a | 89.8±14.7 ab | 39.88±0.30 b | 0.917±0.003 b | 14.47±0.08 b |
T4 | 66.81±1.15 d | 68.9±8.0 b | 14.51±0.15 d | 0.520±0.008 c | 12.04±0.04 e |
T5 | 85.12±1.92 b | 86.0±17.6 ab | 34.52±0.27 c | 0.812±0.006 b | 13.78±0.10 c |
T6 | 63.68±1.54 e | 64.8±10.8 b | 13.68±0.20 d | 0.501±0.005 c | 11.06±0.02 f |
CK | 59.02±1.05 f | 58.4±4.8 b | 9.95±0.09 f | 0.451±0.003 c | 8.97±0.04 g |
指标 Index | 过氧化物酶 POD | 超氧化物岐化酶 SOD | 过氧化氢酶 CAT | 硝酸还原酶 NR | 丙二醛 MDA |
---|---|---|---|---|---|
叶干重 Leaf dry weight | 0.634** | 0.957** | 0.885** | 0.854** | 0.950** |
茎干重 Stem dry weight | 0.635** | 0.920** | 0.938** | 0.866** | 0.928** |
根干重 Root dry weight | 0.646** | 0.973** | 0.923** | 0.866** | 0.962** |
全株鲜干比 Radio of plant fresh to dry weight | -0.639** | -0.916** | -0.892** | -0.846** | -0.938** |
表5 干物质积累量和全株鲜干比与不同酶及丙二醛的相关性
Table 5 Correlation of dry matter accumulation and whole plant fresh to dry ration with different enzymes and malondialdehyde
指标 Index | 过氧化物酶 POD | 超氧化物岐化酶 SOD | 过氧化氢酶 CAT | 硝酸还原酶 NR | 丙二醛 MDA |
---|---|---|---|---|---|
叶干重 Leaf dry weight | 0.634** | 0.957** | 0.885** | 0.854** | 0.950** |
茎干重 Stem dry weight | 0.635** | 0.920** | 0.938** | 0.866** | 0.928** |
根干重 Root dry weight | 0.646** | 0.973** | 0.923** | 0.866** | 0.962** |
全株鲜干比 Radio of plant fresh to dry weight | -0.639** | -0.916** | -0.892** | -0.846** | -0.938** |
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