忍冬叶多糖提取工艺优化及抗菌活性分析

doi:10.13304/j.nykjdb.2023.0312

中国农业科技导报 ›› 2023, Vol. 25 ›› Issue (11): 218-226.DOI: 10.13304/j.nykjdb.2023.0312

• 方法与技术创新 • 上一篇

忍冬叶多糖提取工艺优化及抗菌活性分析

史瑞武¹(), 张素芳², 牛丽娜², 苏田², 赵晓茜³, 那冬晨², 陈伟²()

^1.临汾职业技术学院生命科学系, 山西临汾 041000
^2.山西师范大学生命科学学院, 太原 030000
^3.西北师范大学生命科学学院, 兰州 730070

收稿日期:2023-04-19 接受日期:2023-08-25 出版日期:2023-11-15 发布日期:2023-11-20
通讯作者: 陈伟
作者简介:史瑞武 E-mail：srw0583@163.com；
基金资助:
科技部国家重点研发计划项目(2020YFF0425265);临汾市各地方政府基金项目(2009);山西省应用基础研究计划项目(201901D211403);山西省教育教学“十四五”规划课题(GH-220053);青海省科技计划项目(2022ZY022)

Optimization on Extraction of Polysaccharide from Honeysuckle （Lonicera japonica） Leaves and Its Antibacterial Activity

Ruiwu SHI¹(), Sufang ZHANG², Lina NIU², Tian SU², Xiaoqian ZHAO³, Dongchen NA², Wei CHEN²()

^1.Department of Life Sciences，Linfen Vocational and Technical College，Shanxi Linfen 041000，China
^2.College of Life Science，Shanxi Normal University，Taiyuan 030000，China
^3.College of Life Science，Northwest Normal University，Lanzhou 730070，China

Received:2023-04-19 Accepted:2023-08-25 Online:2023-11-15 Published:2023-11-20
Contact: Wei CHEN

摘要/Abstract

摘要：

为明确忍冬叶多糖体外抑菌及保湿效果，以优化后的水提醇沉法提取忍冬叶多糖，采用预加菌液倾注平板－打孔法，验证忍冬叶多糖对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌的抑菌作用；并利用忍冬叶多糖制备忍冬多糖保湿霜，探究忍冬叶多糖的皮肤保湿效果。结果表明，优化后的提取方法所得的多糖含量为（17.29%±0.41%）。忍冬叶多糖对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌均有抑制作用，对大肠杆菌的抑制效果最佳，平均抑菌圈直径为（15.80±0.58） mm。皮肤水分测试结果表明，忍冬叶多糖具有良好的皮肤补水性及保湿性，具有潜在开发价值。以上研究结果优化了忍冬叶多糖提取工艺，为忍冬的深度开发及提高忍冬附加值奠定了基础。

关键词: 忍冬叶, 多糖, 抑菌试验, 保湿

Abstract:

In order to clarify the antibacterial and moisturizing effects of polysaccharide from honeysuckle leaves in vitro， the polysaccharide was extracted by the optimized water extraction and alcohol precipitation method in this experiment. The antibacterial experiments against Escherichia coli， Bacillus subtilis and Staphylococcusaureus were carried out by the pouring plate with pre-additive bacterial solution and punching method. The polysaccharide from Lonicera japonica leaves was used to prepare the polysaccharide moisturizing cream so as to explore its moisturizing effect. The results showed that the content of polysaccharide was （17.29%±0.41%） using the optimized extraction method. The polysaccharide of Lonicera japonica leaves had inhibitory effects on the 3 bacteria， among them， the inhibitory effect on Escherichia coli was the best， with an average diameter of （15.80±0.58） mm， which was highly sensitive. The skin moisture test showed that the polysaccharide from Lonicera japonica leaves had good skin rehydration and moisturizing properties， which had potential development value. The extraction process of polysaccharides from Lonicera japonica leaves， laying a foundation for the deep development of Lonicera japonica and continuously improving its added value.

Key words: leaves of Lonicera japonica, polysaccharide, antibacterial test, moisturizing

中图分类号:

S377

史瑞武, 张素芳, 牛丽娜, 苏田, 赵晓茜, 那冬晨, 陈伟. 忍冬叶多糖提取工艺优化及抗菌活性分析[J]. 中国农业科技导报, 2023, 25(11): 218-226.

Ruiwu SHI, Sufang ZHANG, Lina NIU, Tian SU, Xiaoqian ZHAO, Dongchen NA, Wei CHEN. Optimization on Extraction of Polysaccharide from Honeysuckle （Lonicera japonica） Leaves and Its Antibacterial Activity[J]. Journal of Agricultural Science and Technology, 2023, 25(11): 218-226.

图/表 6

参考文献 44

1	徐炳声.中国植物志[M].北京:科学出版社, 1988: 1-259.
2	SHANG X F, PAN H, LI M X, et al.. Lonicera japonica Thunb.: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine [J]. J. Ethnopharmacol., 2011, 138(1): 1-21.
3	苏征.不同品种忍冬藤生药学比较研究[D].济南:山东中医药大学, 2018.
	SU Z. Comparative study on pharmacognosy of different varieties of Lonicera japonica [D]. Ji’nan: Shandong University of Traditional Chinese Medicine, 2018.
4	杨绍坤.金银花的化学成分与生物活性研究[J].化工设计通讯, 2019, 45(11): 149-177.
	YANG S K. Studies on chemical constituents and bioactivities of honeysuckle [J]. Chem. Eng. Design Communicat., 2019, 45(11): 149-177.
5	陈两绵, 邹芳艳, 刘晓谦, 等. 3种检测方法比较忍冬药用和非药用部位的抗氧化活性[J]. 世界中医药, 2021, 16(17): 2513-2519, 2526.
	CHEN L M, ZOU F Y, LIU X Q, et al.. Comparison of antioxidant activities between the medicinal parts and non-medicinal parts of lonicera japonica by three test methods [J]. World J. Trad. Chin., 2021, 16(17): 2513-2519, 2526.
6	朱姮, 陈静娴, 文蕾, 等. 忍冬叶化学成分的研究进展[J]. 山东科学, 2016, 29(6): 30-39.
	ZHU H, CHEN J X, WEN L, et al.. Research advances on chemical constituents of leaves from Lonicera japonica Thunb. [J]. Shandong Sci., 2016, 29(6): 30-39.
7	DELATTRE C, FENORADOSOA T A, MICHAUD P. Galactans: an overview of their most important sourcing and applications as natural polysaccharides [J]. Braz. Arch. Biol. Technol., 2011, 54(6): 1075-1092.
8	邓青芳, 周欣, 陈华国. 多糖抗肝损伤作用及其机制研究进展[J]. 中国中药杂志, 2016, 41(16): 2958-2967.
	DENG Q F, ZHOU X, CHEN H G. Advance in study on hepatoprotective effects and its mechanism of polysaccharides [J]. China J. Chin. Materia Med., 2016, 41(16): 2958-2967.
9	邢爽, 方颂平, 姚洪礼, 等. 基于中药、菌菇及茶多糖的研究进展[J]. 北方园艺, 2020(18): 144-151.
	XING S, FANG S P, YAO H L, et al.. Research progress on polysaccharides from chinese herbs, mushroom and tea [J]. Northern Hortic., 2020(18): 144-151.
10	MOHAMMED A S A, NAVEED M, JOST N. Polysaccharides; classification, chemical properties, and future perspective applications in fields of pharmacology and biological medicine (a review of current applications and upcoming potentialities) [J]. J. Polym. Environ., 2021, 29(8): 2359-2371.
11	吕欣, 谭春玲, 麦名娜, 等. 超声辅助酶法提取金银花多糖的工艺优化[J]. 湖南农业科学, 2021 (4): 90-95.
	LYU X, TAN C L, MAI M N, et al.. Process optimization of ultrasonic-assisted aqueous enzymatic method in the extraction of lonicera japonica polysaccharides [J]. Hunan Agric. Sci.,2021(4): 90-95.
12	何金莲. 党参和金银花的活性组分分析[D]. 重庆:重庆医科大学, 2020.
	HE J L. Active component analysis of Codonopsis pilosula and honeysuckle [D]. Chongqing: Chongqing Medical University, 2020.
13	林雄平, 陈晓清, 苏育才, 等. 金银花和苦丁茶多糖提取物抗菌活性研究[J]. 亚热带植物科学, 2008, 98(1): 51-53.
	LIN X P, CHEN X Q, SU Y C, et al.. Antimicrobial activities of polysaccharide extracts from Flos lonicerae and Ilex kudingcha [J]. Subtrop. Plant Sci., 2008, 98 (1): 51-53.
14	殷洪梅, 吕新勇, 萧伟. 金银花多糖的制备工艺优化及免疫活性研究[J]. 中国中药杂志, 2010, 35(4): 453-455.
15	周立, 刘裕红, 贾俊. 复合酶法提取金银花多糖及其抗氧化性[J]. 山东农业大学学报(自然科学版), 2014, 45(5): 646-650.
	ZHOU L, LIU Y H, JIA J. Extraction process of polysaccharide from Lonicera japonica with compound enzyme treatment and its antioxidant activity [J]. J. Shandong Agric. Univ. (Nat. Sci.), 2014, 45(5): 646-650.
16	刘玉国, 刘玉红, 蒋海强. 金银花多糖对小鼠S180肉瘤的抑制作用与机制研究[J]. 肿瘤学杂志, 2012, 18(8): 584-587.
	LIU Y G, LIU Y H, JIANG H Q. Inhibitory effect and mechanism of polysaccharide from Lonicera japonica on mice bearing S180 sarcoma [J]. J. Chin. Oncol., 2012, 18 (8): 584-587.
17	DAL’‍BELO S E, GASPAR L R, MAIA CAMPOS P M. Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques [J]. Skin Res. Technol., 2006, 12(4): 241-246.
18	邓庆华. 用正交试验法优化金银花多糖提取工艺[D]. 长春:东北师范大学, 2008.
	DENG Q H. Optimization of extraction technology of honeysuckle polysaccharide by orthogonal test [D]. Changchun: Northeast Normal University, 2008.
19	林慧钘, 高爱环, 陈煜鑫, 等.七星剑花茎多糖的提取工艺研究及保湿性能测试[J]. 化学工程与技术, 2021, 11(1): 37-44.
	LIN H X, GAO A H, CHEN Y X, et al.. Study on the extraction technology and moisturizing performance of polysaccharide from the stem of Hylocereus undatu [J]. Chem. Eng. Technol., 2021, 11(1): 37-44.
20	谭才邓, 朱美娟, 杜淑霞, 等. 抑菌试验中抑菌圈法的比较研究[J]. 食品工业, 2016, 37(11): 122-125.
	TAN C D, ZHU M J, DU S X, et al.. Study on the inhibition zone method in antimicrobial test [J]. Food Ind., 2016, 37(11): 122-125.
21	翟梅枝, 王磊, 何文君, 等. 核桃青皮乙醇提取物抑菌活性研究[J]. 西北植物学报, 2009, 29(12): 2542-2547.
	ZHAI M Z, WANG L, HE W J, et al.. Antibacterial activity of ethanol extract from walnut green husk [J]. Acta Bot. Bor-Occid. Sin., 2009, 29(12): 2542-2547.
22	王禹博. 链格孢菌SC-018对野慈姑的防除潜力及环境生物安全性评价研究[D]. 沈阳:沈阳农业大学, 2019.
	WANG Y B. Study on the control potential of Alternaria alternata strain SC-018 against Sagittaria trifolia and environmental biosafety assessment [D]. Shenyang: Shenyang Agricultural University, 2019.
23	韩红艳, 冀爱青, 李洪燕. 5种中药材体外抑菌活性研究[J]. 中兽医医药杂志, 2020, 39(2): 63-66.
	HAN H Y, JI A Q, LI H Y. Study on bacteriostasis in vitro activity of five kinds of traditional Chinese medicine [J]. J. Trad. Chin. Vet. Med., 2020, 39(2): 63-66.
24	MORETTIN P A, BUSSAB W O. Estatística Básica [M]. 8th Ed n. SA: Saraiva Educação, 2017.
25	CAPON R J. Extracting value: mechanistic insights into the formation of natural product artifacts—case studies in marine natural products [J]. Nat. Prod. Rep., 2020, 37(1): 55-79.
26	刘冰月. 羊栖菜岩藻多糖的提取分离及其应用研究[D]. 无锡:江南大学, 2017.
	LIU B Y. Extraction, separation and application of polysaccharide from Sargassum fusiforme [D]. Wuxi: Jiangnan University, 2017.
27	高玉杰, 吕海涛. 浒苔多糖和硒化浒苔多糖抑菌作用研究[J]. 食品科技, 2013, 38(1): 195-198, 205.
	GAO Y J, LYU H T. Antimibacterial activities of Enteromorpha polysaccharide and selenium Enteromorpha polysaccharide [J]. Food Sci. Technol., 2013, 38(1): 195-198, 205.
28	PÉTERSZEGI G, ISNARD N, ROBERT A M, et al.. Studies on skin aging. preparation and properties of fucose-rich oligo-and polysaccharides. effect on fibroblast proliferation and survival [J]. Biomed. Pharmacother, 2003, 57(5-6): 187-194.
29	叶翠芳, 王巧利, 刘秋英, 等. 紫菜多糖体外抗紫外线辐射活性研究[J]. 天然产物研究与开发, 2015, 27(4): 641-644, 666.
	YE C F, WANG Q L, LIU Q Y, et al.. Study on the anti-ultraviolet activity of porphyra polysaccharides in vitro [J]. Nat. Prod. Res. Dev., 2015, 27(4): 641-644, 666.
30	陈淑芳, 李明星, 蒙文萍, 等. 海藻多糖在化妆品中的应用研究进展[J]. 海洋科学, 2021, 45(3): 143-151.
	CHEN S F, LI M X, MENG W P, et al.. Application of seaweed polysaccharides in cosmetics [J]. Mar. Sci., 2021, 45(3): 143-151.
31	阳赵艳, 张泽俊, 李浪, 等. 植物多糖在化妆品中的应用[J]. 昭通学院学报,2018, 40(5): 40-43.
	YANG Z Y, ZHANG Z J, LI L, et al.. Application of plant polysaccharide in cosmetics [J]. J. Zhaotong Univ., 2018, 40(5): 40-43.
32	LUAN F, JI Y F, PENG L X, et al.. Extraction, purification, structural characteristics and biological properties of the polysaccharides from Codonopsis pilosula: a review [J/OL]. Carbohyd. Polym., 2021, 261:117863 [2023-03-19]. .
33	ZHOU P Y, EID M, XIONG W F, et al.. Comparative study between cold and hot water extracted polysaccharides from Plantago ovata seed husk by using rheological methods [J/OL]. Food Hydrocolloid, 2020, 101:105465 [2023-03-19]. .
34	王文丽, 张金玲, 魏亚宁, 等. 天然多糖提取、纯化及生物活性研究进展[J]. 食品工业科技,2022, 43(22): 470-480.
	WANG W L, ZHANG J L, WEI Y N, et al.. Extraction, purification and bioactivity of natural polysaccharides: a review [J]. Sci. Technol. Food Ind., 2022, 43(22): 470-480.
35	闫光玲, 张锋, 金娟, 等. 金银花多糖分子量及单糖组成研究[J]. 中国中医药信息杂志, 2019, 26(2): 97-101.
	YAN G L, ZHANG F, JIN J, et al.. Study on molecular weight and monosaccharide composition of polysaccharide from Lonicerae japonicae Flos [J]. Chin. J. Inf. Tradit. Chin. Med., 2019, 26(2): 97-101.
36	刘红萍, 赵琳, 龙源元, 等. 金银花多糖的组成成分与抗氧化活性[J]. 贵州农业科学, 2019, 47(6): 107-112.
	LIU H P, ZHAO L, LONG Y Y, et al.. Components and antioxidant activity of Lonicera japonica polysaccharides [J]. Guizhou Agric. Sci, 2019, 47(6): 107-112.
37	曹珈荣. 乳糖酸对金黄色葡萄球菌抑菌作用研究[D]. 沈阳:沈阳农业大学, 2019.
	CAO J R. Study on the antibacterial effect of lactic acid on Staphylococcus aureus [D]. Shenyang: Shenyang Agricultural University, 2019.
38	BISSETT D L, ANDERSON R L. Genetic evidence for the physiological significance of the D-tagatose 6-phosphate pathway of lactose and D-galactose degradation in Staphylococcus aureus [J]. J. Bacteriol., 1974, 119(3): 698-704.
39	李影影. 几种天然提取物在功能性化妆品中的应用研究[D]. 开封:河南大学, 2016.
	LI Y Y. Application of several natural extracts in functional cosmetics [D]. Kaifeng: Henan University, 2016.
40	王笑月, 刘效兰, 薛燕, 等. 黄精及其复配植物多糖提取工艺优化及人体保湿评价[J]. 食品工业科技, 2018, 39(5): 221-225, 240.
	WANG X Y, LIU X L, XUE Y, et al.. Optimization of process extraction for polysaccharides of Polygonatum and its prescription and evaluation of moisture retention capacity of human body [J]. Sci. Technol. Food Ind., 2018, 39(5): 221-225, 240.
41	郭子叶, 蔡春尔, 耿中雷, 等. 浒苔粗多糖化妆品开发潜力研究[J]. 中国海洋药物, 2014, 33(2): 57-62.
	GUO Z Y, CAI C E, GENG Z L,et al.. Study on potential use of crude polysaccharides from Ulva prolifera in cosmetic [J]. Chin. J. Mar. Drugs, 2014, 33(2): 57-62.
42	任清, 王玢, 李奇, 等. 平菇多糖的提取及其抗氧化保湿功效研究[J]. 香料香精化妆品, 2008 (4): 23-26.
	REN Q, WANG B, LI Q, et al.. Extraction of polysaccharides from Pleurotus ostreatus and study on its antioxidation and moisturizing property [J]. Flavour Fragrance Cosmet., 2008 (4): 23-26.
43	夏诗琪, 王培玲, 张丽, 等. 中草药饲料添加剂的抑菌研究及应用现状[J]. 江西农业大学学报, 2020, 42(6): 1231-1236.
	XIA S Q, WANG P L, ZHANG L, et al.. A study of bacteriostasis of Chinese herbal medicine feed additive and its application status [J]. Acta Agric. Univ. Jiangxiensis, 2020, 42(6): 1231-1236.
44	张超, 毕海林, 陈汇鑫, 等. 中药对动物感染性病原的抑制作用研究[J]. 中兽医医药杂志, 2019, 38(4): 94-96.
	ZHANG C, BI H L, CHEN H X, et al.. Inhibition of traditional Chinese medicine on infectious pathogens in animals [J]. J. Trad. Chin. Vet. Med., 2019, 38(4): 94-96.

成分Component	含量Content	成分 Component	含量Content
乳木果油 Shea butter	3 g	山梨酸钾 Potassium sorbate	0.12 g
蜂蜡Bee wax	1.2 g	甜杏仁油Sweet almond	0.3 mL
鲸蜡硬脂醇 Cetearyl alcohol	7 g	橄榄油Olive oil	0.3 mL
卡波姆Homopolymer	0.6 g	氢化聚葵烯Hydrogenated polysuncene	1 mL
玻尿酸Hyaluronic acid	0.3 g	GTCC	1 mL
柠檬酸Citric acid	0.1 g	甘油Glycerol	2 mL
EDTA-Na₂	0.06 g	神经酰胺Ceramide	8 mL
无水甜菜碱Betaine Anhydrous	3 g	丁二醇1，2-Butanediol	2 mL
海藻糖Trehalose	2.4 g	忍冬叶多糖 Polysaccharide of Lonicera japonica leaves	0.5/1.0 g

成分Component	含量Content	成分 Component	含量Content
乳木果油 Shea butter	3 g	山梨酸钾 Potassium sorbate	0.12 g
蜂蜡Bee wax	1.2 g	甜杏仁油Sweet almond	0.3 mL
鲸蜡硬脂醇 Cetearyl alcohol	7 g	橄榄油Olive oil	0.3 mL
卡波姆Homopolymer	0.6 g	氢化聚葵烯Hydrogenated polysuncene	1 mL
玻尿酸Hyaluronic acid	0.3 g	GTCC	1 mL
柠檬酸Citric acid	0.1 g	甘油Glycerol	2 mL
EDTA-Na₂	0.06 g	神经酰胺Ceramide	8 mL
无水甜菜碱Betaine Anhydrous	3 g	丁二醇1，2-Butanediol	2 mL
海藻糖Trehalose	2.4 g	忍冬叶多糖 Polysaccharide of Lonicera japonica leaves	0.5/1.0 g

菌种 Bacterial species	对照 CK3	试验组 Experimental group
菌种 Bacterial species	对照 CK3	M1	M2	M3	平均值 Mean value
大肠杆菌 Escherichia coli	0.00	16.50	15.50	15.50	15.80±0.58
枯草芽孢杆菌 Bacillus subtilis	0.00	11.50	12.00	14.50	12.70±1.61
金黄色葡萄球菌 Staphylococcus aureus	0.00	13.50	14.00	12.54	13.30±0.74

菌种 Bacterial species	对照 CK3	试验组 Experimental group
菌种 Bacterial species	对照 CK3	M1	M2	M3	平均值 Mean value
大肠杆菌 Escherichia coli	0.00	16.50	15.50	15.50	15.80±0.58
枯草芽孢杆菌 Bacillus subtilis	0.00	11.50	12.00	14.50	12.70±1.61
金黄色葡萄球菌 Staphylococcus aureus	0.00	13.50	14.00	12.54	13.30±0.74

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[10]	杨晓斌,张腾,李华*. 养殖鲆鲽类免疫增强剂的研究进展[J]. , 2013, 15(6): 46-54.
[11]	孙晓晨,初晓宇,伍宁丰. 细菌Ⅰ型普鲁兰酶的研究进展[J]. , 2012, 14(2): 73-80.
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Optimization on Extraction of Polysaccharide from Honeysuckle （Lonicera japonica） Leaves and Its Antibacterial Activity

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