Application and Prospect of Virus-induced Gene Silencing in Crop Gene Function Research

doi:10.13304/j.nykjdb.2020.0781

Abstract

Abstract:

With the completion of crop genome sequencing， a large number of genes' functions affecting important agronomic traits of crops are waiting to be discovered. Due to the lack of genetic transformation methods， the research on gene function of most crops is progressing slowly. Virus-induced gene silencing （VIGS） technology does not rely on genetic transformation. Through virus vector inoculation， efficient silencing of targeted genes can be achieved in contemporary plants. VIGS technology is increasingly used in reverse genetics for analysis of gene functions and metabolic pathways in different crops due to its fast onset time， high silencing efficiency， low operating cost， high-throughput and wide application of plants. This article introduced the technical principles and development process of VIGS， systematically summarized the application of VIGS in different crop research， and then summarized and discussed the limitations of existing VIGS applications and several key factors affecting its silence efficiency， and finally the application in future plant biology research was prospected， which would provide reference for its further application and development.

Key words: virus-induced gene silencing （VIGS）, viral vector, crop, gene function, reverse genetics

摘要：

随着作物基因组测序工作的陆续完成，大量影响作物重要农艺性状的基因功能等待挖掘。由于缺少高效的遗传转化方法，多种作物的基因功能研究进展缓慢。病毒诱导的基因沉默（virus-induced gene silencing， VIGS）技术不依赖遗传转化，通过病毒载体接种即可在当代植物体内实现对靶向基因的沉默。VIGS技术因其起效时间快、沉默效率高、操作成本低、便于高通量和应用植物范围广等特点，被越来越多地应用于不同作物基因功能和代谢通路解析的反向遗传学研究中。介绍了VIGS的技术原理及其发展过程，系统归纳了VIGS在不同作物中的应用，总结和讨论了现有VIGS应用的局限性以及影响其沉默效率的几个关键因素，并对VIGS技术在未来植物生物学研究中的应用进行了展望，以期为VIGS技术的进一步应用和发展提供参考。

关键词: 病毒诱导的基因沉默, 病毒载体, 作物, 基因功能, 反向遗传学

CLC Number:

Q943.2

Mengyuan HAO, Qi HANG, Gongyao SHI. Application and Prospect of Virus-induced Gene Silencing in Crop Gene Function Research[J]. Journal of Agricultural Science and Technology, 2022, 24(1): 1-13.

郝梦媛, 杭琦, 师恭曜. VIGS基因沉默技术在作物基因功能研究中的应用与展望[J]. 中国农业科技导报, 2022, 24(1): 1-13.

Figures/Tables 3

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