Establishment of Comprehensive Evaluation and Prediction Equation for Cucumber Wet-cold Tolerance at Seedling Stage

doi:10.13304/j.nykjdb.2021.0484

Abstract

Abstract:

To establish the evaluation system of cucumber on wet-cold tolerance， the high humidity and low temperature environment was set in growth chambers. The morphological and physiological indexes of 20 cucumber varieties at seedling stage were determined for evaluating the wet-cold tolerance. And the prediction equation of cucumber wet-cold tolerance was established by principal component， membership function and stepwise regression analysis. The results showed that the prediction equation of cucumber wet-cold tolerance was established： Y=0.492+0.032 I₆-0.084 I₄-0.029 I₇， in which， Y represented the comprehensive value， I₄， I₆ and I₇ represented the relative values of catalase activity， proline content and polyphenol oxidase activity in the high humidity and low temperature environment and normal environment， respectively. The higher the Y value was， the more tolerant to wet-cold the cucumber would be. Validation analysis showed that the average accuracy of the prediction equation was 95.9%. It showed that the prediction equation could be used to evaluate wet-cold tolerance of cucumber， which provided technique supports for wet-cold tolerance breeding and cultivation of cucumber.

Key words: cucumber, wet-cold tolerance, identification index, prediction equation

摘要：

为建立黄瓜耐湿冷鉴定评价体系，利用人工气候箱设置湿冷环境处理，通过主成分析和隶属函数分析筛选黄瓜幼苗期耐湿冷性的形态和生理指标，通过逐步回归建立耐湿冷性预测方程。结果表明，黄瓜苗期耐湿冷性方程为：Y=0.492+0.032 I₆-0.084 I₄-0.029 I₇，其中，Y代表综合耐湿冷能力，其值越高表明该品种的耐湿冷性越强；I₄、I₆和I₇分别代表相对过氧化氢酶活性、相对脯氨酸含量和相对多酚氧化酶活性。验证分析表明，该预测方程的平均精确度为95.9%，可用于黄瓜耐湿冷性评价，为黄瓜耐湿冷育种和设施栽培提供了技术支持。

关键词: 黄瓜, 耐湿冷性, 鉴定指标, 预测方程

CLC Number:

S642.2

Yuanyuan DING, Xi’ao WANG, Ce LIU, Shuju LI, Zhihui CHENG. Establishment of Comprehensive Evaluation and Prediction Equation for Cucumber Wet-cold Tolerance at Seedling Stage[J]. Journal of Agricultural Science and Technology, 2022, 24(11): 87-96.

丁圆圆, 王曦奥, 刘策, 李淑菊, 程智慧. 黄瓜耐湿冷性苗期综合评价预测方程的建立[J]. 中国农业科技导报, 2022, 24(11): 87-96.

Figures/Tables 9

Table 1 Information of test materials

缩写

Abbreviation

品种

Variety

来源

Source

耐湿冷性

Wet-cold tolerance

Zn26

中农26

Zhongnong 26

中国农业科学院蔬菜花卉研究所

Institute of Vegetable and Flower， Chinese Academy of Agricultural Sceinces

中 Moderate

Syz

三叶早

Sanyezao

泰安禾元有限公司

Taian Heyuan Co.， Ltd.

敏感 Sensitive

Jyys

京研优胜

Jingyanyousheng

国家蔬菜工程中心

National Vegetable Engineering Center

中 Moderate

QsF1

秋胜F1

Qiusheng F1

吉林环球有限公司

Jilin Huanqiu Co.， Ltd.

弱 Weak

Ymt2188

雅美特2188

Yameite 2188

寿光欣欣然有限公司

Shouguang Xinxinran Co.， Ltd.

弱 Weak

FyF1

富阳F1

Fuyang F1

寿光洪亮有限公司

Shouguang Hongliang Co.， Ltd.

弱 Weak

Jy41*

津优41

Jinyou 41

天津科润黄瓜所

Tianjin Kerun Cucumber Institute

敏感 Sensitive

Table 1 Information of test materials

缩写

Abbreviation

品种

Variety

来源

Source

耐湿冷性

Wet-cold tolerance

Jy4*

津优4号

Jinyou 4

天津科润黄瓜所

Tianjin Kerun Cucumber Institute

中 Moderate

Zn28*

中农28

Zhongnong 28

中科院蔬菜花卉研究所

Institute of Vegetable and Flower， Chinese Academy of Agricultural Sceinces

强 High

Hl35

荷兰35

Helan 35

寿光宏伟种业有限公司

Shouguang Hongwei Co.， Ltd.

弱 Weak

Sy4

疏研4号

Shuyan 4

湖南农科所

Hunan Academy of Agricultural Sciences

敏感 Sensitive

Jy1

津优1号

Jinyou 1

天津科润黄瓜研究所

Tianjin Kerun Cucumber Institute

强 High

Jy64

津优64号

Jinyou 64

天津科润黄瓜研究所

Tianjin Kerun Cucumber Institute

强 High

Bn5

博耐5号

Bonai 5

天津德瑞特种业有限公司

Tianjin Derit Co.， Ltd.

强 High

Msjp313

妙收金品313

Miaoshoujinpin 313

北京妙收有限公司

Beijing Miaoshou Co.， Ltd.

敏感 Sensitive

Ccmc

长春密刺

Changchunmici

沈阳谷雨有限公司

Shenyang Guyu Co.， Ltd.

中 Moderate

Jy35*

津优35

Jinyou 35

天津科润黄瓜研究所

Tianjin Kerun Cucumber Institute

弱 Weak

Rg2*

瑞光2号

Ruiguang 2

国家蔬菜工程中心

National Vegetable Engineering Center

弱 Weak

Sy11*

疏研11号

Shuyan 11

湖南农科所

Hunan Academy of Agricultural Sciences

敏感 Sensitive

Drt8-9F*

德瑞特8-9F

Deruite 8-9F

天津德瑞特有限公司

Tianjin Derit Co.， Ltd.

中 Moderate

Table 2 Relative values of different indexes under wet?cold treatment of different cucumber varieties

品种 Variety	过氧化氢酶 CAT	苯丙氨酸解氨酶 PAL	多酚氧化酶PPO	超氧阴离子 $O 2 -$	谷胱甘肽 GSH	可溶性糖 SS	脯氨酸 Pro	株高 SH	茎粗 SD	叶长 LL
Zn26	0.93±0.012 d	1.35±0.095 cdef	1.01±0.053 gh	1.73±0.107 bcd	1.58±0.077 c	1.32±0.062 ab	3.94±0.078 c	0.67±0.029 abc	0.89±0.078 abc	0.98±0.008 a
Syz	0.62±0.088 e	0.96±0.021 g	0.70±0.057 i	1.25±0.098 gh	1.48±0.098 c	0.61±0.049 g	1.80±0.074 d	0.59±0.017 bc	0.82±0.016 bc	0.71±0.037 c
FyF1	1.22±0.061 c	1.00±0.033 g	1.372±0.029 ef	2.17±0.065 a	1.12±0.056 d	0.90±0.042 ef	3.73±0.402 c	0.64±0.024 abc	0.92±0.029 ab	0.90±0.024 ab
Sy4	1.28±0.120 c	1.48±0.119 bcd	2.50±0.045 b	1.99±0.082 ab	1.42±0.081 c	1.24±0.087 bc	1.13±0.087 e	0.59±0.008 bc	0.88±0.024 abc	0.89±0.017 ab
Jy64	2.17±0.049 a	1.38±0.115 cde	1.47±0.041 de	1.04±0.133 hi	2.58±0.082 a	0.85±0.021 efg	5.45±0.093 a	0.73±0.024 ab	0.96±0.024 a	0.83±0.029 bc
Bn5	0.58±0.061 e	1.05±0.107 fg	1.52±0.029 de	1.26±0.075 fgh	1.54±0.074 c	1.54±0.066 a	5.37±0.107 a	0.78±0.024 a	0.90±0.037 abc	0.90±0.024 ab
Hl35	1.11±0.049 cd	1.70±0.119 b	3.44±0.172 a	1.53±0.050 def	0.33±0.021 f	0.98±0.049 cde	3.47±0.021 bc	0.61±0.033 bc	0.81±0.016 bc	0.89±0.005 ab
Ccmc	1.07±0.074 cd	2.59±0.049 a	2.06±0.078 c	1.45±0.054 efg	0.76±0.025 e	0.85±0.021 efg	3.77±0.021 bc	0.71±0.016 ab	0.94±0.029 ab	0.98±0.021 a
Jy1	0.59±0.039 e	1.05±0.033 g	1.66d±0.057 d	1.03±0.021 hi	2.12±0.024 b	1.31±0.071 ab	4.61±0.062 b	0.74±0.033 ab	0.87±0.012 abc	0.89±0.012 ab
Ymt2188	1.11±0.080 cd	1.09±0.020 efg	0.56±0.070 i	1.82±0.017 bc	1.01±0.050 d	1.27±0.155 b	1.22±0.049 e	0.52±0.026 c	0.80±0.088 c	0.85±0.057 b
QsF1	1.57±0.078 b	1.08±0.045 efg	1.11±0.012 fg	1.66±0.086 cde	1.17±0.046 d	0.67±0.087 fg	3.68±0.050 bc	0.67±0.045 ab	0.85±0.034 abc	0.82±0.057 b
Jyys	0.96±0.069 d	1.51±0.091 bc	1.20±0.066 fg	1.25±0.033 fgh	1.24±0.008 d	0.90±0.021 def	3.36±0.109 c	0.70±0.057 ab	0.89±0.033 abc	0.85±0.005 b
Msjp313	1.59±0.091 b	1.21±0.086 defg	0.80±0.029 hi	1.86±0.036 bc	1.03±0.033 d	1.17±0.070 bcd	1.05±0.037 e	0.60±0.049 bc	0.87±0.037 abc	0.86±0.037 b
均值Mean	1.13	1.34	1.50	1.55	1.35	1.04	3.25	0.65	0.87	0.87
标准差SD	0.43	0.44	0.78	0.38	0.58	0.28	1.49	0.08	0.06	0.07
变异系数CV/%	38.05	32.84	52.00	24.52	42.96	26.92	45.85	12.31	6.90	8.04

Table 2 Relative values of different indexes under wet?cold treatment of different cucumber varieties

品种 Variety	过氧化氢酶 CAT	苯丙氨酸解氨酶 PAL	多酚氧化酶PPO	超氧阴离子 $O 2 -$	谷胱甘肽 GSH	可溶性糖 SS	脯氨酸 Pro	株高 SH	茎粗 SD	叶长 LL
Zn26	0.93±0.012 d	1.35±0.095 cdef	1.01±0.053 gh	1.73±0.107 bcd	1.58±0.077 c	1.32±0.062 ab	3.94±0.078 c	0.67±0.029 abc	0.89±0.078 abc	0.98±0.008 a
Syz	0.62±0.088 e	0.96±0.021 g	0.70±0.057 i	1.25±0.098 gh	1.48±0.098 c	0.61±0.049 g	1.80±0.074 d	0.59±0.017 bc	0.82±0.016 bc	0.71±0.037 c
FyF1	1.22±0.061 c	1.00±0.033 g	1.372±0.029 ef	2.17±0.065 a	1.12±0.056 d	0.90±0.042 ef	3.73±0.402 c	0.64±0.024 abc	0.92±0.029 ab	0.90±0.024 ab
Sy4	1.28±0.120 c	1.48±0.119 bcd	2.50±0.045 b	1.99±0.082 ab	1.42±0.081 c	1.24±0.087 bc	1.13±0.087 e	0.59±0.008 bc	0.88±0.024 abc	0.89±0.017 ab
Jy64	2.17±0.049 a	1.38±0.115 cde	1.47±0.041 de	1.04±0.133 hi	2.58±0.082 a	0.85±0.021 efg	5.45±0.093 a	0.73±0.024 ab	0.96±0.024 a	0.83±0.029 bc
Bn5	0.58±0.061 e	1.05±0.107 fg	1.52±0.029 de	1.26±0.075 fgh	1.54±0.074 c	1.54±0.066 a	5.37±0.107 a	0.78±0.024 a	0.90±0.037 abc	0.90±0.024 ab
Hl35	1.11±0.049 cd	1.70±0.119 b	3.44±0.172 a	1.53±0.050 def	0.33±0.021 f	0.98±0.049 cde	3.47±0.021 bc	0.61±0.033 bc	0.81±0.016 bc	0.89±0.005 ab
Ccmc	1.07±0.074 cd	2.59±0.049 a	2.06±0.078 c	1.45±0.054 efg	0.76±0.025 e	0.85±0.021 efg	3.77±0.021 bc	0.71±0.016 ab	0.94±0.029 ab	0.98±0.021 a
Jy1	0.59±0.039 e	1.05±0.033 g	1.66d±0.057 d	1.03±0.021 hi	2.12±0.024 b	1.31±0.071 ab	4.61±0.062 b	0.74±0.033 ab	0.87±0.012 abc	0.89±0.012 ab
Ymt2188	1.11±0.080 cd	1.09±0.020 efg	0.56±0.070 i	1.82±0.017 bc	1.01±0.050 d	1.27±0.155 b	1.22±0.049 e	0.52±0.026 c	0.80±0.088 c	0.85±0.057 b
QsF1	1.57±0.078 b	1.08±0.045 efg	1.11±0.012 fg	1.66±0.086 cde	1.17±0.046 d	0.67±0.087 fg	3.68±0.050 bc	0.67±0.045 ab	0.85±0.034 abc	0.82±0.057 b
Jyys	0.96±0.069 d	1.51±0.091 bc	1.20±0.066 fg	1.25±0.033 fgh	1.24±0.008 d	0.90±0.021 def	3.36±0.109 c	0.70±0.057 ab	0.89±0.033 abc	0.85±0.005 b
Msjp313	1.59±0.091 b	1.21±0.086 defg	0.80±0.029 hi	1.86±0.036 bc	1.03±0.033 d	1.17±0.070 bcd	1.05±0.037 e	0.60±0.049 bc	0.87±0.037 abc	0.86±0.037 b
均值Mean	1.13	1.34	1.50	1.55	1.35	1.04	3.25	0.65	0.87	0.87
标准差SD	0.43	0.44	0.78	0.38	0.58	0.28	1.49	0.08	0.06	0.07
变异系数CV/%	38.05	32.84	52.00	24.52	42.96	26.92	45.85	12.31	6.90	8.04

Table 3 Correlation analysis of 10 wet?cold tolerance identification indexes

指标Index	过氧化氢酶CAT	苯丙氨酸解氨酶PAL	多酚氧化酶PPO	超氧阴离子O $2 -$	谷胱甘肽GSH	可溶性糖SS	脯氨酸 Pro	株高 SH	茎粗 SD
苯丙氨酸解氨酶PAL	-0.175
多酚氧化酶PPO	-0.258	0.504^**
超氧阴离子O $2 -$	0.397^*	-0.053	0.178
谷胱甘肽GSH	-0.395^*	v0.414^*	-0.564^**	-0.519^**
可溶性糖SS	-0.379^*	-0.181	-0.022	-0.141	0.16
脯氨酸Pro	-0.794^**	0.036	-0.144	-0.579^*	0.442^**	0.125
株高SH	-0.661^**	0.032	0.070	-0.505^**	0.457^**	0.269	0.796^**
茎粗SD	-0.528^**	0.274	-0.228	0.055	0.411^*	0.303	0.524^**	0.498^*
叶长LL	-0.524^**	0.304	0.312	0.259	0.407^*	0.286	0.204	0.407^*	0.462^*

Table 3 Correlation analysis of 10 wet?cold tolerance identification indexes

指标Index	过氧化氢酶CAT	苯丙氨酸解氨酶PAL	多酚氧化酶PPO	超氧阴离子O $2 -$	谷胱甘肽GSH	可溶性糖SS	脯氨酸 Pro	株高 SH	茎粗 SD
苯丙氨酸解氨酶PAL	-0.175
多酚氧化酶PPO	-0.258	0.504^**
超氧阴离子O $2 -$	0.397^*	-0.053	0.178
谷胱甘肽GSH	-0.395^*	v0.414^*	-0.564^**	-0.519^**
可溶性糖SS	-0.379^*	-0.181	-0.022	-0.141	0.16
脯氨酸Pro	-0.794^**	0.036	-0.144	-0.579^*	0.442^**	0.125
株高SH	-0.661^**	0.032	0.070	-0.505^**	0.457^**	0.269	0.796^**
茎粗SD	-0.528^**	0.274	-0.228	0.055	0.411^*	0.303	0.524^**	0.498^*
叶长LL	-0.524^**	0.304	0.312	0.259	0.407^*	0.286	0.204	0.407^*	0.462^*

Table 4 Principal component analysis and eigenvalue

指标 Index	第一主成分PC1 （X₁）	第二主成分PC2（X₂）	第三主成分PC3（X₃）	第四主成分PC4（X₄）
株高SH（I₁）	0.876	0.275	-0.067	-0.173
茎粗SD（I₂）	0.751	0.274	0.431	0.200
叶长LL（I₃）	0.671	-0.015	0.146	0.387
过氧化氢酶CAT（I₄）	-0.481	0.112	0.623	-0.512
谷胱甘肽GSH（I₅）	0.292	0.784	0.017	-0.084
脯氨酸Pro（I₆）	0.840	0.242	-0.137	-0.206
多酚氧化酶PPO（I₇）	0.406	-0.464	-0.593	-0.155
可溶性糖SS（I₈）	-0.433	0.437	-0.430	0.571
超氧阴离子O $2 -$ （I₉）	0.140	-0.395	0.520	0.572
苯丙氨酸解氨酶PAL（I₁₀）	0.621	-0.616	0.047	-0.124
特征值Eigenvalue	3.560	1.779	1.429	1.224
贡献率Contribution rate/%	36.601	17.786	14.285	12.240
累计贡献率Accumulative contribution rate/%	36.601	54.387	68.672	80.912

Table 4 Principal component analysis and eigenvalue

指标 Index	第一主成分PC1 （X₁）	第二主成分PC2（X₂）	第三主成分PC3（X₃）	第四主成分PC4（X₄）
株高SH（I₁）	0.876	0.275	-0.067	-0.173
茎粗SD（I₂）	0.751	0.274	0.431	0.200
叶长LL（I₃）	0.671	-0.015	0.146	0.387
过氧化氢酶CAT（I₄）	-0.481	0.112	0.623	-0.512
谷胱甘肽GSH（I₅）	0.292	0.784	0.017	-0.084
脯氨酸Pro（I₆）	0.840	0.242	-0.137	-0.206
多酚氧化酶PPO（I₇）	0.406	-0.464	-0.593	-0.155
可溶性糖SS（I₈）	-0.433	0.437	-0.430	0.571
超氧阴离子O $2 -$ （I₉）	0.140	-0.395	0.520	0.572
苯丙氨酸解氨酶PAL（I₁₀）	0.621	-0.616	0.047	-0.124
特征值Eigenvalue	3.560	1.779	1.429	1.224
贡献率Contribution rate/%	36.601	17.786	14.285	12.240
累计贡献率Accumulative contribution rate/%	36.601	54.387	68.672	80.912

Table 5 Index of each variety and ranking

品种 Variety	X₁	X₂	X₃	X₄	U（X₁）	U（X₂）	U（X₃）	U（X₄）	D	排序Ranking
权重 W	—	—	—	—	0.446	0.223	0.179	0.152	—	—
QsF1	6.989	2.041	-1.475	-0.335	0.369	0.663	0.659	0.664	0.531	8
Syz	5.887	0.006	0.030	-0.195	0.371	0.340	0.369	0.342	0.359	13
Hl35	6.663	-1.327	-1.609	0.131	0.445	0.340	0.357	0.709	0.446	10
Bn5	7.375	1.813	-0.969	0.133	0.666	0.646	0.666	0.336	0.611	1
Zn26	8.234	2.245	-0.743	-0.468	0.584	0.351	0.660	0.646	0.555	6
Sy4	6.026	1.694	0.487	0.921	0.361	0.436	0.397	0.608	0.422	11
Jy1	2.826	1.601	0.880	-0.959	0.592	0.625	0.639	0.450	0.586	3
Jy64	1.770	2.087	-0.233	1.597	0.617	0.594	0.646	0.509	0.601	2
Jyys	4.958	1.305	1.817	0.289	0.663	0.543	0.438	0.445	0.563	4
Ymt2188	4.449	-0.530	-0.490	1.043	0.426	0.597	0.657	0.666	0.542	7
Ccmc	7.477	-0.774	-0.562	0.110	0.654	0.430	0.663	0.346	0.559	5
Msjp313	2.073	1.079	1.618	0.948	0.350	0.480	0.347	0.358	0.380	12
FyF1	5.425	0.445	-0.400	0.120	0.506	0.395	0.625	0.548	0.508	9

Fig. 1 Cluster analysis of 13 cucumber varieties based on D valueNote： The abbreviations of varieties are same to Table 1.

Table 6 Relative values of indexes of 7 tested varieties

指标 Index	Jy41	Jy4	Zn28	Jy35	Rg2	Sy11	Drt8-9F
株高SH	0.532	0.62	0.530	0.623	0.515	0.667	0.243
茎粗SD	0.759	0.814	0.746	0.793	0.790	0.855	0.608
叶长LL	0.892	0.814	0.277	0.462	0.368	0.781	0.663
过氧化氢酶活性CAT	0.418	0.155	0.468	0.488	0.467	0.580	3.574
谷胱甘肽含量GSH	0.627	1.804	1.074	1.069	1.341	2.908	1.569
脯氨酸含量Pro	2.069	2.489	3.685	1.783	3.798	3.629	1.322
多酚氧化酶活性PPO	1.258	0.743	2.507	0.657	1.117	1.003	0.958
可溶性糖含量SS	1.896	2.703	1.311	1.480	2.021	1.781	1.996
超氧阴离子含量O $2 -$	1.524	2.252	1.339	0.977	1.016	2.949	0.744
苯丙氨酸解氨酶活性PAL	1.602	0.716	0.883	0.978	0.985	1.401	2.687

Table 6 Relative values of indexes of 7 tested varieties

指标 Index	Jy41	Jy4	Zn28	Jy35	Rg2	Sy11	Drt8-9F
株高SH	0.532	0.62	0.530	0.623	0.515	0.667	0.243
茎粗SD	0.759	0.814	0.746	0.793	0.790	0.855	0.608
叶长LL	0.892	0.814	0.277	0.462	0.368	0.781	0.663
过氧化氢酶活性CAT	0.418	0.155	0.468	0.488	0.467	0.580	3.574
谷胱甘肽含量GSH	0.627	1.804	1.074	1.069	1.341	2.908	1.569
脯氨酸含量Pro	2.069	2.489	3.685	1.783	3.798	3.629	1.322
多酚氧化酶活性PPO	1.258	0.743	2.507	0.657	1.117	1.003	0.958
可溶性糖含量SS	1.896	2.703	1.311	1.480	2.021	1.781	1.996
超氧阴离子含量O $2 -$	1.524	2.252	1.339	0.977	1.016	2.949	0.744
苯丙氨酸解氨酶活性PAL	1.602	0.716	0.883	0.978	0.985	1.401	2.687

Table 7 Evaluation accuracy of equation

品种Variety	原始值D	预测值P	差值Difference	准确度Accuracy/%
Jy41	0.439	0.457	0.018	95.9
Jy4	0.528	0.537	0.009	98.3
Zn28	0.560	0.598	0.038	93.2
Jy35	0.515	0.490	-0.025	95.1
Rg2	0.494	0.522	0.028	94.3
Sy11	0.460	0.430	-0.030	93.5
Drt8-9F	0.556	0.526	-0.030	94.6

References 30

1	陈启林,山仑,程智慧,等.低温弱光对黄瓜类囊体膜耦联状况的影响[J].西北农业大学学报,2000,28(6):6-11.
	CHEN Q L, SHAN L, CHENG Z H, et al.. Effect of low temperature and low light treatment on the coupling state of cucumber thylakoid membrane [J]. Acta Univ. Agric. Boreali-Occident, 2000, 28(6):6-11.
2	IKKONEN E N, SHIBAEVA T G, TITOVITOV A F. Influence of daily short term temperature drops on respiration to photosynthesis ratio in chilling sensitive plants [J]. Russ. J. Plant Physiol., 2018, 65(1):78-83.
3	徐艳玲.不同湿度条件对温室黄瓜二氧化碳施肥状态下光合效率的影响[J].中国科技信息,2009,11(2):72-73.
	XU Y L. Effects of different humidity conditions on photosynthetic efficiency of greenhouse cucumber under carbon dioxide fertilization [J]. China Sci. Tech. Inform., 2009, 11(2):72-73.
4	SUZUKI M, UMEDA H, MATSUO S, et al.. Effects of relative humidity and nutrient supply on growth and nutrient uptake in greenhouse tomato production [J]. Sci. Hortic., 2015, 187(10):44-49.
5	SERNA L, FENOLL C. Reinforcing the idea of signaling in the stomatal pathway [J]. Trends Genetics, 2002, 18(12):597-600.
6	CASSON S A, FRANKLIN K A, GRAY J E, et al.. Phytochrome b and pif4 regulate stomatal development in response to light quantity [J]. Curr. Biol., 2008, 19(3):229-234.
7	KUDOYAROVA G R, VESELOV D S, FAIZOV R G, et al.. Stomatal response to changes in temperature and humidity in wheat cultivars grown under contrasting climatic conditions [J]. Russ. J. Plant Physiol., 2007, 54(1):46-49.
8	张爽,董然,董妍.不同空气相对湿度对槭叶草生长及光合生理特性的影响[J].东北林业大学学报,2014,42(3):24-36.
	ZHANG S, DONG R, DONG Y. Effects of Relative air humidity on the growth and photosynthesis of Mukdenia rossii [J]. J. Northeast For. Univ., 2014, 42(3):24-36.
9	FRANKS P J, FARQUHAR G D. The mechanical diversity of stoma and its significance in gas exchange control [J]. Plant Physiol., 2007, 143(1):78-87.
10	ALINIAEIFARD S, VAN MEETEREN U. Can prolonged exposure to low VPD disturb the aba signaling in stomatal guard cells? [J]. J. Exp. Bot., 2014, 64(12):3551-3566.
11	王慧,李梅兰,许建平,等.基于冠层温湿度模型的日光温室黄瓜霜霉病预警方法[J].应用生态学报,2015,26(10):3027-3034.
	WANG H, LI M L, XU J P, et al.. An early warning method of cucumber downy mildew in solar greenhouse based on canopy temperature and humidity modeling [J]. Chin. J. Appl. Ecol., 2015, 26(10):3027-3034.
12	GU W, HUANG L, WEI J, et al.. Comparative proteomics analysis of developing seed of preharvest seed deterioration resistant soybean cultivar under high temperature and humidity stress [J]. Curr. Proteomics, 2015, 12(3):168-184.
13	徐超,杨再强,王明田,等.高温环境下空气湿度对花期番茄生长及根系分泌的调节作用[J].中国农业气象,2020,41(9):552-563.
	XU C, YANG Z Q, WANG M T, et al.. Regulating effect of air humidity on growth and root secretion of tomato at flowering stage in high temperature environment [J]. Chin. J. Agrometeorol., 2020, 41(9):552-563.
14	蒋洪涛,蒋夏兰,商贺阳,等.利用多元统计分析方法评价甘蔗新品系的抗旱性[J]. 中国糖料,2021,43(3):1-7.
	JIANG H T, JIANG X L, SHANG H Y, et al.. Evaluation of drought resistance of new sugarcane strains using multivariate statistical analysis method [J]. Sug. Crops of Chin., 2021, 43(3):1-7.
15	杨燕君,杨复康,宋永宏,等.山西不同杏品种抗寒性主成分分析[J].山西农业科学,2020,48(12):1913-1915, 1963.
	YANG Y J, YANG F K, SONG Y H, et al.. Principal component analysis of cold resistance of different apricot varieties in Shanxi [J]. J. Shanxi Agric. Sci., 2020, 48(12):1913-1915, 1963.
16	王焱,蔡伟,兰剑,等. 12个苜蓿品种抗旱性综合评价[J].草原与草坪,2018,38(2):80-88.
	WANG Y, CAI W, LAN J, et al.. Comprehensive evaluation of drought resistance of 12 alfalfa varieties [J]. Grassland Turf., 2018, 38(2):80-88.
17	李彦慧,咚爱民,刘冬云.廊坊杨抗寒性研究[J].河北农业大学学报,2005,28(4):23-26.
	LI Y H, DONG A M, LIU D Y. Studies on cold resistance of popular Langfang [J]. J. Agric. Univ. Hebei, 2005, 28(4):23-26.
18	苏秀红,强胜,宋小玲.不同地理种群紫茎泽兰耐热性差异的比较分析[J].西北植物学报,2005,25(9):1766-1771.
	SU X H, QIANG S, SONG X L. Comparison of Eupatorium adenophorum heat tolerances of different geographical populations [J]. Acta Bot. Bor-Occid. Sin., 2005, 25(9):1776-1771.
19	柴守玺.小麦抗早生态分类中适合性聚类方法的研究[J].应用生态学报,2000,11(6):833-838.
	CHAI S X. Cluster analysis methods appropriate for classification of drought-resistant wheat ecotypes [J]. Chin. J. Appl. Ecol., 2000, 11(6):833-838.
20	周广生,梅方竹,周竹青.小麦不同品种耐湿性生理指标综合评价及其预测[J].中国农业科学,2003,36(11):1378-1382.
	ZHOU G S, MEI F Z, ZHOU Z Q. Comprehensive evaluation and forecast on physiological indices of waterlogging resistance of different wheat varieties [J]. Sci. Agric. Sin., 2003, 36(11):1378-1382.
21	曹翠玲,麻鹏达.植物生理学教学实验指导[M].杨凌:西北农林科技大学出版社,2016:20-25.
	CAO C L, MA P D. The Instruction of Plant Physiology Teaching Experiment [M]. Yangling: Northwest Agriculture and Forestry University Press, 2016:20-25.
22	李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:1-49.
	LI H S. Principles and Techniques of Plant Physiology and Biochemistry Experiments [M]. Beijing: Higher Education Press, 2000:1-49.
23	刘雪梅.黄瓜,辣椒品种早期低温弱光耐受性评价方法研究[D].北京:中国农业科学院,2010.
	LIU X M. Evaluation method of low temperature and poor light tolerance of cucumber and pepper cultivars at early development stage [D]. Beijing: Chinese Academy of Agricultural Sciences, 2010.
24	刘宏宇,姚雪,栾非时,等.厚皮甜瓜种子萌芽期耐冷性评价方法研究[J].东北农业大学学报,2016,47(7):24-31.
	LIU H Y, YAO X, LUAN F S, et al.. Study on evaluation method of chilling tolerance at germination stage in Cucumis melo [J]. J. Northeast Agric. Univ., 2016, 47(7):24-31.
25	XU Z Z, ZHOU G S, HAN G X, et al. Photosynthetic potential and its association with lipid peroxidation in response to high temperature at different leaf ages in Maize [J]. J. Plant Growth Regul., 2011, 30(1): 41-50.
26	李合生.现代植物生理学[M].北京:高等教育出版社,2012:1-74.
	LI H S. Modern Plant Physiology [M]. Beijing: Higher Education Press, 2012:1-74.
27	向婷颖,田茂燕,钟川,等.砧用茄子种质耐冷性鉴定及耐性生理响应[J].南方农业学报,2021,52(1):163-171.
	XIANG T Y, TIAN M Y, ZHONG C, et al.. Identification of cold resistance and resistance physiological response of eggplant germplasms for rootstock [J]. J. Southern Agric., 2021, 52(1):163-171.
28	张红颖,向春阳,曹高燚,等.玉米杂交种芽苗期耐寒性的鉴定[J].天津农学院学报,2015,22(1):15-18.
	ZHANG H Y, XIANG C Y, CAO G Y, et al.. Identification of chilling tolerance in maize hybrids at germination and seedling growth stage [J]. J. Tianjin Agric. Coll., 2015, 22(1):15-18.
29	郑昀晔,胡晋,张胜,等.玉米自交系发芽期和苗期耐寒性的鉴定[J].浙江大学学报(农业与生命科学版),2006,32(1):41-45.
	ZHENG J Y, HU J, ZHANG S, et al.. Identification of chilling tolerance in maize inbred lines at germination and seedling growth stage [J]. J. Zhejiang Univ. (Agric. Life Sci.), 2006, 32(1):41-45.
30	但忠,木万福,苏银玲.欧洲型黄瓜成株期耐热性鉴定指标的筛选及预测方程的建立[J].西南农业学报,2015,28(5):2213-2217.
	DAN Z, MU W F, SU Y L. Determination of heat-tolerance evaluation and establishment of mathematical evaluation model in adult stage of European type cucumber under natural high temperature [J]. Southwest China J. Agric. Sci., 2015, 28(5):2213-2217.

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