Effect of Chitosan on Alfalfa’s Cold Resistance and Content of Cold-resistant Protective Substances

doi:10.13304/j.nykjdb.2021.0622

Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (2): 192-198.DOI: 10.13304/j.nykjdb.2021.0622

• BIO-MANUFACTURING & RESOURCE AND ECOLOGY • Previous Articles

Effect of Chitosan on Alfalfa’s Cold Resistance and Content of Cold-resistant Protective Substances

Ze TENG¹(), Yuxia ZHANG¹^,²(), Weidong CHEN¹, Baiming CONG³, Yonglei TIAN⁴, Qingxin ZHANG¹, Yongliang ZHANG¹, Dongru WANG⁵

^1.College of Agriculture，Inner Mongolia University for Nationalities，Inner Mongolia Tongliao 028041，China
^2.Institute of Ecological Environment and Green Development，Inner Mongolia University for Nationalities，Inner Mongolia Tongliao 028000，China
^3.Institute of Animal Husbandry and Veterinary Science of Tongliao City，Inner Mongolia Autonomous Region，Inner Mongolia Tongliao 028000，China
^4.Inner Mongolia Autonomous Region Academy of Industrial Sciences，Hohhot 010010，China
^5.Agricultural and Animal Husbandry Technology Extension Center of Inner Mongolia Autonomous Region，Hohhot 010010，China

Received:2021-07-28 Accepted:2021-12-09 Online:2023-02-15 Published:2023-05-17
Contact: Yuxia ZHANG

壳聚糖对苜蓿抗寒性及抗寒保护物质含量的影响

滕泽¹(), 张玉霞¹^,²(), 陈卫东¹, 丛百明³, 田永雷⁴, 张庆昕¹, 张永亮¹, 王东儒⁵

^1.内蒙古民族大学农学院，内蒙古通辽 028041
^2.内蒙古民族大学生态环境与绿色发展研究所，内蒙古通辽 028000
^3.内蒙古自治区通辽市畜牧兽医科学研究所，内蒙古通辽 028000
^4.内蒙古自治区农牧业科学院，呼和浩特 010010
^5.内蒙古自治区农牧技术推广中心，呼和浩特 010010

通讯作者: 张玉霞
作者简介:滕泽 E-mail：tengzzze@qq.com；
基金资助:
内蒙古自治区自然科学研究基金项目(2020MS03081);国家自然科学基金项目(31960352);内蒙古自治区科技计划项目(2021GG0109);国家牧草产业技术体系(CARS-34)

Abstract

Abstract:

In order to explore the response of alfalfa cold resistance to chitosan application level in Horqin sandy land， taking ‘Gongnong 1’ alfalfa variety as the material， a single factor randomized block experiment design was adopted， and 0， 50，100， 150， 200 μg·g^-1 amount of chitosan were sprayed in autumn. The alfalfa overwintering materials were treated under simulated low-temperature freezing conditions （–20 ℃） and cold storage （4 ℃）， and the changes of relative conductivity of alfalfa root collar and the contents of soluble protein， proline， sucrose and fructose were detected. The results showed that under the condition of low temperature freezing， when treated with 100 μg·g^-1 chitosan， the relative conductivity of alfalfa root collar was the lowest， which was 31.49%， while the contents of proline， sucrose and fructose were the highest， which were 8.05，88.17^{and 86.32 mg·g^-1， respectively.When treated with 150 μg·g^-1 chitosan， the soluble protein content of alfalfa root collar was the highest， which was 27.19 mg·g^-1. The results of correlation analysis showed that the relationships between soluble protein， proline， sucrose， fructose and relative conductivity were negative under different low temperature stress treatments. Under the condition of low temperature freezing stress， chitosan could reduce the relative conductivity and improve the cold resistance of alfalfa by increasing the contents of soluble protein， proline， sucrose and fructose in the root collar of alfalfa. Spraying 100~150 μg·g^-1 chitosan in Horqin sandy land in autumn was conducive to the safe overwintering of alfalfa.}

Key words: chitosan, alfalfa, low temperature stress, cold resistance

摘要：

为探究科尔沁沙地紫花苜蓿抗寒性对壳聚糖施用水平的响应，以‘公农1号’紫花苜蓿为材料，采用单因素随机区组试验设计，于秋季喷施含量为0、50、100、150、200 μg·g^-1的壳聚糖，将紫花苜蓿越冬材料模拟低温冷冻条件（-20 ℃）和冷藏（4 ℃）处理，检测紫花苜蓿根颈的相对电导率以及可溶性蛋白、脯氨酸、蔗糖和果糖含量的变化。结果表明，在低温冷冻条件下，100 μg·g^-1壳聚糖处理时，紫花苜蓿根颈的相对电导率最低，为31.49%，而脯氨酸、蔗糖和果糖含量均为最高，分别为8.05、88.17和86.32 μg·g^-1；150 μg·g^-1壳聚糖处理时，紫花苜蓿根颈的可溶性蛋白含量最高，为27.19 mg·g^-1。相关性分析结果表明，不同低温胁迫处理下紫花苜蓿根颈中可溶性蛋白、脯氨酸、蔗糖、果糖与相对电导率的关系均为负相关。在低温冷冻胁迫条件下，壳聚糖通过提高苜蓿根颈的可溶性蛋白、脯氨酸、蔗糖、果糖含量，可以降低相对电导率，提高苜蓿抗寒性。在科尔沁沙地秋季喷施100~150 μg·g^-1壳聚糖有利于苜蓿安全越冬。

关键词: 壳聚糖, 紫花苜蓿, 低温胁迫, 抗寒性

CLC Number:

S541

Ze TENG, Yuxia ZHANG, Weidong CHEN, Baiming CONG, Yonglei TIAN, Qingxin ZHANG, Yongliang ZHANG, Dongru WANG. Effect of Chitosan on Alfalfa’s Cold Resistance and Content of Cold-resistant Protective Substances[J]. Journal of Agricultural Science and Technology, 2023, 25(2): 192-198.

滕泽, 张玉霞, 陈卫东, 丛百明, 田永雷, 张庆昕, 张永亮, 王东儒. 壳聚糖对苜蓿抗寒性及抗寒保护物质含量的影响[J]. 中国农业科技导报, 2023, 25(2): 192-198.

Figures/Tables 6

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喷施量 Spray amount/（μg·g^-1）	相对电导率 Relative conductivity/%
喷施量 Spray amount/（μg·g^-1）	低温冷藏 Low temperature refrigeration	低温冷冻 Low temperature freezing
0	16.82±1.28 aβ	38.77±2.85 bcα
50	16.46±1.09 abβ	40.70±0.79 bα
100	15.06±3.21 abcβ	31.49±1.15 dα
150	12.60±0.17 bcβ	35.80±0.93 cα
200	11.93±1.53 cβ	44.61±2.00 aα

喷施量 Spray amount/（μg·g^-1）	相对电导率 Relative conductivity/%
喷施量 Spray amount/（μg·g^-1）	低温冷藏 Low temperature refrigeration	低温冷冻 Low temperature freezing
0	16.82±1.28 aβ	38.77±2.85 bcα
50	16.46±1.09 abβ	40.70±0.79 bα
100	15.06±3.21 abcβ	31.49±1.15 dα
150	12.60±0.17 bcβ	35.80±0.93 cα
200	11.93±1.53 cβ	44.61±2.00 aα

喷施量 Spray amount/（μg·g^-1）	可溶性蛋白 The soluble protein/（mg·g^-1）
喷施量 Spray amount/（μg·g^-1）	低温冷藏 Low temperature refrigeration	低温冷冻 Low temperature freezing
0	19.70±0.98 cα	21.27±0.47 bα
50	20.15±0.15 bcβ	22.95±0.61 bα
100	24.25±0.48 aα	25.53±0.99 aα
150	20.36±1.26 bcβ	27.19±1.24 aα
200	21.81±1.05 bα	22.06±0.67 bα

喷施量 Spray amount/（μg·g^-1）	可溶性蛋白 The soluble protein/（mg·g^-1）
喷施量 Spray amount/（μg·g^-1）	低温冷藏 Low temperature refrigeration	低温冷冻 Low temperature freezing
0	19.70±0.98 cα	21.27±0.47 bα
50	20.15±0.15 bcβ	22.95±0.61 bα
100	24.25±0.48 aα	25.53±0.99 aα
150	20.36±1.26 bcβ	27.19±1.24 aα
200	21.81±1.05 bα	22.06±0.67 bα

喷施量 Spray amount/（μg·g^-1）	脯氨酸含量 Proline content/（μg·g^-1）
喷施量 Spray amount/（μg·g^-1）	低温冷藏 Low temperature refrigeration	低温冷冻 Low temperature freezing
0	5.88±0.55 bα	6.04±0.26 cα
50	5.91±0.45 bβ	6.86±0.64 bcα
100	7.55±0.26 aα	8.05±0.60 aα
150	7.17±0.25 aα	7.93±0.43 aα
200	7.03±0.28 aα	7.05±0.24 bα

Effect of Chitosan on Alfalfa’s Cold Resistance and Content of Cold-resistant Protective Substances

壳聚糖对苜蓿抗寒性及抗寒保护物质含量的影响

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Figures/Tables 6

References 22

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温度处理 Temperature treatment	测定指标 Determination index	相对电导率Relative conductivity	可溶性蛋白Soluble protein	脯氨酸 Proline	蔗糖 Sucrose
低温冷藏 Low temperature refrigeration	相对电导率Relative conductivity	1.00
	可溶性蛋白Soluble protein	-0.23	1.00
	脯氨酸 Proline	-0.69	0.79	1.00
	蔗糖 Sucrose	-0.65	0.72	0.95^**	1.00
	果糖 Fructose	-0.05	-0.04	0.32	0.31
低温冷冻 Low temperature freezing	相对电导率Relative conductivity	1.00
	可溶性蛋白Soluble protein	-0.70	1.00
	脯氨酸 Proline	-0.71	0.85^*	1.00
	蔗糖 Sucrose	-0.78	0.69	0.86^*	1.00
	果糖 Fructose	-0.72	0.49	0.77	0.97^**

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