Physiological Response and Cold Resistance Evaluation of Cyperus esculentus Germplasms Under Low Temperature Stress

doi:10.13304/j.nykjdb.2022.0132

Abstract

Abstract:

Low temperature is one of the important environmental factors affecting the cultivation of Cyperus esculentus in the agro-pastoral region of northern China. In order to clarify the main identification indexes and screen out the germplasm resources of Cyperus esculentus with strong low temperature tolerance， 18 Cyperus esculentus germplasms from different ecological regions were used to identify and evaluate their low temperature tolerance. The effect of low temperature stress on seedling emergence was studied in 3 consecutive years of late spring chilling. 3， 5， 7 and 9 ℃ were set to simulate the minimum temperature of late spring chilling， and seed germination related indexes and seedling stress resistance physiological indexes were measured to study the cold resistance. The results showed that with the decrease of temperature， germination index， seed vigor index and chlorophyll content in leaves decreased generally， relative membrane permeability increased， the contents of malonaldehyde， soluble sugar and free proline were gradually accumulated， and the activities of superoxide dismutase and peroxidase increased in the mass. Based on field test， laboratory germination test and physiological and biochemical indexes of seedlings， 18 germplasms were classified into 5 categories： strong cold resistance （HLJ105， RY126， BY156 and HLJ106）， cold resistance （RY102 and BY141）， moderate cold resistance （RY114， NM103， XJ101， JL154， LN181 and SX100）， weak cold resistance （HB122 and HN162）， and no cold resistance （QY186， GD187， XY109 and ZYS01）. The results provided basic resources and theoretical basis for the germplasms innovation and cultivars breeding of low temperature resistant Cyperus esculentus.

Key words: Cyperus esculentus, late spring chilling, low temperature stress, seedling physiological responses, cold resistance evaluation

摘要：

低温是影响我国北方农牧交错区油莎豆种植的重要环境因子之一。为明确油莎豆耐低温主要鉴定指标并筛选耐低温较强的油莎豆种质资源，对不同生态区的18份油莎豆种质资源进行耐低温鉴定和评价。大田试验连续3年研究了倒春寒天气导致的低温胁迫对油莎豆出苗的影响，室内试验模拟倒春寒日最低温度范围设定了3、5、7、9 ℃共4个温度处理，测定低温处理后种子发芽相关指标和幼苗抗逆生理指标。结果表明，随着处理温度的逐渐降低，发芽指数、种子活力指数和叶片中叶绿素含量大致呈降低趋势，丙二醛不断积累，相对膜透性逐渐增大，可溶性糖、游离脯氨酸含量逐渐积累，超氧化物歧化酶和过氧化物酶活性总体上不断增强。通过大田试验、室内发芽试验及幼苗生理生化指标测定，综合评价将18份种质资源分为5组：HLJ105、RY126、BY156和HLJ106为强抗寒性种质材料，RY102和BY141为抗寒性材料，RY114、NM103、XJ101、JL154、LN181和SX100为中抗寒性材料，HB122和HN162为弱抗寒性材料，QY186、GD187、XY109和ZYS01为不抗寒性材料。研究结果为耐低温油莎豆种质资源创新和新品种培育提供了基础材料和理论依据。

关键词: 油莎豆, 倒春寒, 低温胁迫, 幼苗生理响应, 抗寒性评价

CLC Number:

S565

Peng ZHONG, Lili MIAO, Jianli WANG, Jie LIU, Xiaolong WANG. Physiological Response and Cold Resistance Evaluation of Cyperus esculentus Germplasms Under Low Temperature Stress[J]. Journal of Agricultural Science and Technology, 2023, 25(9): 83-96.

钟鹏, 苗丽丽, 王建丽, 刘杰, 王晓龙. 油莎豆种质资源低温胁迫生理响应与耐寒性评价[J]. 中国农业科技导报, 2023, 25(9): 83-96.

Figures/Tables 12

References 30

1	路战远, 刘和, 张建中, 等. 油莎豆产业发展现状、问题与建议[J]. 现代农业, 2019(6):11-13.
	LU Z Y, LIU H, ZHANG J Z, et al.. Current situation, problems and suggestions on the industrial development of Cyperus esculentus [J]. Mod. Agric., 2019 (6):11-13.
2	CODINA-TORRELLA I, GUAMIS B, TRUJULLOA J. Characterization and comparison of tiger nuts (Cyperus esculentus L.) from different geographical origin: physico-chemical characteristics and protein fractionation [J]. Ind. Crops Prod., 2015, 65( 93): 406-414.
3	刘奕含. 油莎豆种植密度试验[J]. 农村科技, 2019(2):16-18.
4	张学昆. 我国油莎豆产业研发进展报告[J]. 中国农村科技, 2019 (4):67-69.
5	王小丽, 裴玉贺, 郭新梅, 等. 低温胁迫下玉米幼苗的几种生理生化指标的变化[J]. 植物生理学通讯, 2009,45 (5):487-490.
	WANG X L, PEI Y H, GUO X M, et al.. Effect of low temperature stress on some physiological and biochemical indexes of maize seedlings [J]. Plant Physiol. Commun., 2009, 45(5): 487-490.
6	唐月异, 王传堂, 高华援, 等. 花生种子吸胀期间耐低温性及其与品质性状的相关研究 [J]. 核农学报, 2011, 25(3):436-442.
	TANG Y Y, WANG C T, GAO H Y, et al.. Low temperature tolerance during seed imbibition and its relationship to main quality traits in peanut [J]. Acta Agric. Nucl. Sin., 2011, 25(3): 436-442.
7	常博文, 钟鹏, 刘杰, 等. 低温胁迫和赤霉素对花生种子萌发和幼苗生理响应的影响[J]. 作物学报, 2019, 45(1):118-130.
	CHANG B W, ZHONG P, LIU J, et al.. Effect of low-temperature stress and gibberellin on seed germination and seedling physiological responses in peanut [J]. Acta Agron. Sin., 2019, 45(1):118-130.
8	王宏燕, 陈琳, 马晓伟, 等. 低温胁迫下木醋液对水稻幼苗抗寒性的影响[J]. 东北农业大学学报, 2019, 50(2): 1-8.
	WANG H Y, CHEN L, MA X W, et al.. Effect of wood vinegar on cold resistance of rice seedlings under low temperature stress [J]. J. Northeast Agric. Univ., 2019, 50(2): 1-8.
9	董万鹏, 罗充, 龙秀琴, 等. 低温胁迫对西番莲抗寒生理指标的影响[J]. 植物生理学报, 2015, 51(5):771-777.
	DONG W P, LUO C, LONG X Q, et al.. Effects of low temperature stress on physiological indexes of cold resistance of passifl ora edulis [J]. Plant Physiol. J., 2015, 51 (5):771-777.
10	田雲, 蒋景龙, 沈季雪, 等. 低温胁迫对5个栽培品种黄瓜生长及生理指标的影响[J]. 分子植物育种, 2017, 15(5):1938-1944.
	TIAN Y, JIANG J L, SHEN J X, et al.. Effect of low temperature stress on growth and physiological indexes of five cultivars of cucumber [J]. Mol. Plant Breeding, 2017, 15(5):1938-1944.
11	杨帆, 朱文学. 油莎豆研究现状及展望[J]. 粮食与油脂, 2020, 33(7):4-6.
	YANG F, ZHU W X. Research status and prospect of Cyperus esculentus [J].Grain Fats, 2020, 33(7): 4-6.
12	王瑞元, 王晓松, 相海. 一种多用途的新兴油料作物——油莎豆[J]. 中国油脂, 2019, 44(1): 1-4.
13	陈星, 陈滴, 刘蕾. 油莎豆全成分分析[J]. 食品科技, 2009, 34(3):165-168.
	CHEN X, CHEN D, LIU L. Analysis of total components in Cyperus esculentus [J]. Food Technol., 2009,34(3):165-168.
14	王盈希, 吴苏喜, 周利平, 等. 油莎豆品质分析及加工利用研究进展[J]. 食品工业, 2020, 41(10):273-276.
	WANG Y X, WU S X, ZHOU L P, et al.. Research progress on quality analysis, processing and utilization of Cyperus esculentus [J]. Food Ind.,2020, 41(10): 273-276.
15	于海英, 柴守权, 周玉江, 等. 倒春寒与杨树烂皮病发病的关系[J]. 浙江农林大学学报, 2011, 28(6): 927-930.
	YU H Y, CHAI S Q, ZHOU Y J, et al.. Relationship between valsa sordida and abnormal coldness in spring [J]. J. Zhejiang A&F Univ., 2011, 28(6): 927-930.
16	杨文刚, 黄永学, 刘可群, 等. 倒春寒气象指标及等级划分研究[J]. 湖北农业科学, 2018, 57(15):49-53.
	YANG W G, HUANG Y X, LIU K Q, et al.. Study on the meteorological index and grading of late spring coldness [J]. J. Hubei Agric. Sci., 2018, 57(15):49-53.
17	李春娟, 闫彩霞, 张廷婷, 等. 温度对不同花生品种种子活力的影响[J]. 花生学报, 2012, 41(1): 21-25.
	LI C J, YAN C X, ZHANG T T, et al.. Effect of temperature on vigor of peanut seed and quality components [J]. J. Peanut Sci., 2012, 41(1): 21-25.
18	黄金堂, 陈海玲, 李清华, 等. 春花生与秋花生种子活力比较研究[J]. 花生学报, 2007,36(3): 30-33.
	HUANG J T, CHEN H L, LI Q H, et al.. The comparative study of seed vigor between spring planted and autumn planted peanuts [J]. J. Peanut Sci., 2007, 36(3): 30-33.
19	张宪政. 植物叶绿素含量测定——丙酮乙醇混合液法[J]. 辽宁农业科学, 1986(3):26-28.
20	李锦树, 王洪春, 王文英, 等. 干旱对玉米叶片细胞透性及膜脂的影响[J]. 植物生理学报, 1983, 9(3): 9-15.
	LI J S, WANG H C, WANG W Y, et al.. Effect of drought on the permeability and membrane lipid composition from maize leaves [J]. Acta Phytophysiol. Sin., 1983, 9(3): 9-15.
21	GUO X R, CHANG B W, ZU Y G, et al.. The impacts of increased nitrate supply on catharanthus roseus growth and alkaloid accumulations under ultraviolet-B stress [J]. J. Plant Interact, 2014, 9: 640-646.
22	GIANNOPOLITIS C N, RIES S K. Superoxide dismutases: Ⅰ. occurrence in higher plants [J]. Plant Physiol.,1977, 59(2) : 309-314.
23	KLAPHECK S, ZIMMER I, COSSSE H. Scavenging of hydrogen peroxidein the endosperm of ricinus communis by ascorbate peroxidase [J]. Plant Cell Physiol., 1990,31(7) : 1005-1013.
24	刘慧民, 仉茜, 苏青, 等. 18种绣线菊苗期抗寒性评价与筛选[J]. 园艺学报, 2014, 41(12):2427-2436.
	LIU H M, ZHANG Q, SU Q, et al.. Studies on evaluation and screening of cold resistance during seedling stage in eighteen species or varieties of spiraea [J]. Acta Hortic. Sin., 2014, 41(12):2427-2436.
25	张文娥, 王飞, 潘学军. 应用隶属函数法综合评价葡萄种间抗寒性[J]. 果树学报, 2007, 24(6):849-853.
	ZHANG W E, WANG F, PAN X J. Comprehensive evaluation on cold hardiness of vitis species by subordinate function (SF) [J]. J. Fruit Sci., 2007, 24(6):849-853.
26	陈涛, 张华, 安黎哲. ICE1-CBF信号通路在植物抵御低温胁迫和调控发育过程中的作用研究进展[J]. 西北植物学报, 2019, 39(8):1513-1520.
	CHEN T, ZHANG H, AN L Z. Research progress of ICE1-CBF pathway and its function in plant cold tolerance and development [J]. Acta Bot. Bor-Occid. Sin., 2019, 39(8):1513-1520.
27	王芳, 王淇, 赵曦阳. 低温胁迫下植物的表型及生理响应机制研究进展[J]. 分子植物育种, 2019, 17(15):5144-5153.
	WANG F, WANG Q, ZHAO X Y. Research progress of phenotype and physiological response mechanism of plants under low temperature stress [J]. Mol. Plant Breeding, 2019, 17(15):5144-5153.
28	史普想, 王铭伦, 于洪波, 等. 不同成熟度花生种子萌动期低温对苗期生长发育的影响[J]. 作物杂志, 2009(1):78-81.
	SHI P X, WANG M L, YU H B, et al.. Effects of low temperature at germination stage on seedlings of peanuts with different maturation degree [J]. Crops,2009(1):78-81.
29	钟鹏, 刘杰, 王建丽,等. 花生对低温胁迫的生理响应及抗寒性评价[J]. 核农学报, 2018, 32(6): 1195-1202.
	ZHONG P, LIU J, WANG J L, et al.. Physiological responses and cold resistance evaluation of peanut under low-temperature stress [J]. Acta Agric. Nucl. Sin., 2018, 32(6): 1195-1202.
30	全怡吉, 樊仙, 李如丹,等. 不同甘蔗品种对低温胁迫的生理响应及耐寒性综合评价[J]. 热带作物学报, 2020, 41(1):63-68.
	QUAN Y J, FAN X, LI R D, et al.. Integrative assessments on physiological response and cold tolerance of different sugarcane varieties to low temperature [J]. Chin. J. Trop. Crops, 2020, 41(1):63-68.

种质材料 Germplasm	种质来源 Origin	出苗率 Seedling emergence rate/%
种质材料 Germplasm	种质来源 Origin	2018	2019	2020
HLJ105	黑龙江Heilongjiang	95.18±1.33 a	96.94±2.26 a	93.52±3.34 a
RY126	俄罗斯Russia	93.22±1.68 a	97.82±1.57 a	92.84±3.18 a
BY156	保加利亚Bulgaria	93.14±1.52 a	95.73±2.04 a	91.08±1.69 ab
HLJ106	黑龙江Heilongjiang	90.16±1.69 a	92.48±3.38 a	88.87±4.52 ab
RY102	俄罗斯Russia	89.44±1.87 a	91.92±2.11 b	85.52±5.64 abc
BY141	保加利亚Bulgaria	88.02±2.26 ab	86.61±3.18 bcd	83.16±1.87 bc
RY114	俄罗斯Russia	86.69±2.84 bc	85.16±4.97 bcd	82.15±6.62 cd
NM103	内蒙古Inner Mongolia	83.17±1.89 bcd	83.94±1.49 cd	85.91±3.58 abc
XJ101	新疆Xinjiang	80.35±3.34 cd	78.82±3.37 de	86.13±2.49 abc
JL154	吉林Jilin	80.11±2.66 cd	82.25±4.42 cde	72.15±5.47 d
LN181	辽宁Liaoning	78.10±2.15 de	75.48±3.55 ef	70.38±6.11 d
SX100	山西Shanxi	72.64±1.51 e	70.63±4.77 f	73.64±3.42 d
HB122	河北Hebei	65.54±4.43 f	66.12±2.58 f	62.04±4.97 e
HN162	河南Henan	53.13±3.37 g	55.47±6.83 g	50.35±7.74 f
ZYS01	湖北Hubei	45.87±5.52 hi	48.96±7.24 h	41.18±7.26 g
XY109	湖南Hunan	44.69±3.96 i	43.32±6.08 h	43.51±6.83 g
GD187	广东Guangdong	42.35±6.65 i	43.17±5.56 h	40.12±5.44 g
QY186	海南Hainan	40.06±7.84 i	41.66±6.84 h	38.68±8.85 g

种质材料 Germplasm	种质来源 Origin	出苗率 Seedling emergence rate/%
种质材料 Germplasm	种质来源 Origin	2018	2019	2020
HLJ105	黑龙江Heilongjiang	95.18±1.33 a	96.94±2.26 a	93.52±3.34 a
RY126	俄罗斯Russia	93.22±1.68 a	97.82±1.57 a	92.84±3.18 a
BY156	保加利亚Bulgaria	93.14±1.52 a	95.73±2.04 a	91.08±1.69 ab
HLJ106	黑龙江Heilongjiang	90.16±1.69 a	92.48±3.38 a	88.87±4.52 ab
RY102	俄罗斯Russia	89.44±1.87 a	91.92±2.11 b	85.52±5.64 abc
BY141	保加利亚Bulgaria	88.02±2.26 ab	86.61±3.18 bcd	83.16±1.87 bc
RY114	俄罗斯Russia	86.69±2.84 bc	85.16±4.97 bcd	82.15±6.62 cd
NM103	内蒙古Inner Mongolia	83.17±1.89 bcd	83.94±1.49 cd	85.91±3.58 abc
XJ101	新疆Xinjiang	80.35±3.34 cd	78.82±3.37 de	86.13±2.49 abc
JL154	吉林Jilin	80.11±2.66 cd	82.25±4.42 cde	72.15±5.47 d
LN181	辽宁Liaoning	78.10±2.15 de	75.48±3.55 ef	70.38±6.11 d
SX100	山西Shanxi	72.64±1.51 e	70.63±4.77 f	73.64±3.42 d
HB122	河北Hebei	65.54±4.43 f	66.12±2.58 f	62.04±4.97 e
HN162	河南Henan	53.13±3.37 g	55.47±6.83 g	50.35±7.74 f
ZYS01	湖北Hubei	45.87±5.52 hi	48.96±7.24 h	41.18±7.26 g
XY109	湖南Hunan	44.69±3.96 i	43.32±6.08 h	43.51±6.83 g
GD187	广东Guangdong	42.35±6.65 i	43.17±5.56 h	40.12±5.44 g
QY186	海南Hainan	40.06±7.84 i	41.66±6.84 h	38.68±8.85 g

种质材料 Germplasm	对照 Control	处理Treatment
种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
HLJ105	21.45±1.68 ab	13.16±1.22 a	15.54±1.42 a	18.66±1.68 a	20.87±1.62 ab
RY126	22.08±2.32 ab	12.58±1.34 a	14.87±1.53 a	18.24±1.62 ab	21.56±1.77 a
BY156	21.14±1.54 ab	12.44±1.06 a	14.82±1.44 a	17.92±1.54 ab	20.69±1.39 ab
HLJ106	22.96±1.86 a	13.81±1.55 a	15.73±1.38 a	18.79±1.39 a	21.84±1.84 a
RY102	20.13±3.31 ab	11.01±1.18 b	13.20±1.32 b	17.55±1.66 ab	20.01±1.58 ab
BY141	21.54±3.27 ab	10.25±1.11 bc	13.06±1.26 b	17.23±1.52 ab	20.78±1.46 ab
RY114	21.67±2.52 ab	10.54±1.26 bc	12.11±1.24 bc	15.97±1.46 c	21.23±1.72 a
NM103	22.55±1.98 a	11.36±1.07 b	12.08±1.30 bc	17.59±1.37 ab	21.97±1.35 a
XJ101	19.94±2.11 ab	9.54±1.02 c	11.54±1.29 bcd	16.32±1.25 c	19.64±1.26 b
JL154	20.86±1.60 ab	9.68±1.02 c	10.67±1.17 de	17.69±1.44 ab	20.25±1.80 ab
LN181	20.53±2.73 ab	10.23±1.13 bc	11.34±1.43 cd	16.67±1.22 bc	20.14±1.74 ab
SX100	19.81±2.47 ab	9.94±1.07 bc	10.25±1.38 de	15.98±1.34 c	19.63±1.69 b
HB122	20.53±1.65 ab	10.23±1.15 bc	11.09±1.21cd	16.52±1.09 c	19.98±1.65 b
HN162	21.25±2.20 ab	9.06±1.14 cd	10.52±1.15 de	17.34±1.17 ab	20.85±1.52 ab
ZYS01	22.30±3.37 ab	8.87±1.09 d	9.66±1.07 e	15.50±1.58 c	21.29±1.38 a
XY109	19.11±3.05 b	7.65±1.00 d	9.51±1.18 e	16.23±1.33 c	19.08±1.22 b
GD187	19.62±1.88 ab	8.22±1.01 d	9.90±1.02 e	16.08±1.19 c	19.24±1.66 b
QY186	21.87±2.04 ab	7.54±1.05 d	9.29±1.11 e	15.91±1.84 c	21.10±1.51 a

种质材料 Germplasm	对照 Control	处理Treatment
种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
HLJ105	21.45±1.68 ab	13.16±1.22 a	15.54±1.42 a	18.66±1.68 a	20.87±1.62 ab
RY126	22.08±2.32 ab	12.58±1.34 a	14.87±1.53 a	18.24±1.62 ab	21.56±1.77 a
BY156	21.14±1.54 ab	12.44±1.06 a	14.82±1.44 a	17.92±1.54 ab	20.69±1.39 ab
HLJ106	22.96±1.86 a	13.81±1.55 a	15.73±1.38 a	18.79±1.39 a	21.84±1.84 a
RY102	20.13±3.31 ab	11.01±1.18 b	13.20±1.32 b	17.55±1.66 ab	20.01±1.58 ab
BY141	21.54±3.27 ab	10.25±1.11 bc	13.06±1.26 b	17.23±1.52 ab	20.78±1.46 ab
RY114	21.67±2.52 ab	10.54±1.26 bc	12.11±1.24 bc	15.97±1.46 c	21.23±1.72 a
NM103	22.55±1.98 a	11.36±1.07 b	12.08±1.30 bc	17.59±1.37 ab	21.97±1.35 a
XJ101	19.94±2.11 ab	9.54±1.02 c	11.54±1.29 bcd	16.32±1.25 c	19.64±1.26 b
JL154	20.86±1.60 ab	9.68±1.02 c	10.67±1.17 de	17.69±1.44 ab	20.25±1.80 ab
LN181	20.53±2.73 ab	10.23±1.13 bc	11.34±1.43 cd	16.67±1.22 bc	20.14±1.74 ab
SX100	19.81±2.47 ab	9.94±1.07 bc	10.25±1.38 de	15.98±1.34 c	19.63±1.69 b
HB122	20.53±1.65 ab	10.23±1.15 bc	11.09±1.21cd	16.52±1.09 c	19.98±1.65 b
HN162	21.25±2.20 ab	9.06±1.14 cd	10.52±1.15 de	17.34±1.17 ab	20.85±1.52 ab
ZYS01	22.30±3.37 ab	8.87±1.09 d	9.66±1.07 e	15.50±1.58 c	21.29±1.38 a
XY109	19.11±3.05 b	7.65±1.00 d	9.51±1.18 e	16.23±1.33 c	19.08±1.22 b
GD187	19.62±1.88 ab	8.22±1.01 d	9.90±1.02 e	16.08±1.19 c	19.24±1.66 b
QY186	21.87±2.04 ab	7.54±1.05 d	9.29±1.11 e	15.91±1.84 c	21.10±1.51 a

种质材料 Germplasm	对照 Control	处理Treatment
种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
HLJ105	116.42±10.24 a	72.16±5.87 a	85.47±5.45 a	105.81±8.24 a	114.19±8.24 a
RY126	115.37±9.68 ab	73.54±4.62 a	84.12±6.17 a	106.11±9.12 a	111.58±9.92 a
BY156	106.83±9.36 ab	70.08±5.42 a	84.87±5.54 a	101.54±7.37 ab	103.92±6.88 ab
HLJ106	109.58±8.47 ab	71.14±6.03 a	83.95±6.35 a	104.32±6.24 a	106.55±10.37 ab
RY102	112.46±6.69 ab	63.44±5.29 b	83.01±5.81 a	103.36±7.08 ab	109.87±9.75 ab
BY141	102.17±10.55 b	60.12±4.67 b	82.16±4.68 a	98.45±6.51 abc	99.82±5.38 c
RY114	98.84±11.64 b	62.94±4.33 b	73.66±5.22 b	91.24±6.33 c	95.46±6.51 c
NM103	100.25±10.23 b	58.93±3.69 bc	70.18±4.91 bc	96.52±4.48 c	96.17±5.84 c
XJ101	97.89±8.64 b	61.88±5.22 b	72.64±6.14 bc	89.95±5.29 c	93.61±8.26 c
JL154	98.15±8.87 b	56.81±4.14 cd	70.88±6.03 bc	91.23±6.17 c	93.54±7.95 c
LN181	114.37±9.22 ab	54.27±4.61 cd	69.87±5.29 bc	102.39±4.62 ab	108.57±8.83 ab
SX100	104.28±10.05 b	52.39±3.85 d	71.03±5.07 bc	94.67±5.83 c	100.63±9.07 bc
HB122	106.42±9.06 ab	53.97±4.07 c	68.24±6.23 bc	96.58±6.19 b	104.87±8.15 ab
HN162	101.09±9.94 b	57.04±3.15 cd	66.57±5.16 c	94.66±4.55 c	97.85±4.58 c
ZYS01	111.04±8.98 ab	45.51±4.10 e	60.15±4.54 d	99.47±3.87 abc	104.52±5.09 ab
XY109	98.83±10.51 b	43.67±3.94 e	58.07±4.68 d	93.53±5.46 c	97.19±8.66 c
GD187	85.47±10.24 c	42.55±3.16 e	56.69±5.00 d	74.56±6.86 d	83.14±6.10 d
QY186	95.33±9.76 b	43.90±2.68 e	57.32±4.27 d	91.12±5.04 c	94.07±5.94 c