Effects of Modified Biochar on Soil Nematode Community in Cadmium and Lead Contaminated Soil

doi:10.13304/j.nykjdb.2023.0321

Abstract

Abstract:

To study the effects of repeated application of modified biochar on cadmium and lead contaminated agricultural soil and nematode community， using modified biochar as the material， a located experiment was conducted for 2， 3， 4 and 5 years of continuous application of modified biochar. Cadmium and lead in wheat grains and soil were determined to explore the sustainability of its effect. Meanwhile， the effect of modified biochar on soil nematode community was studied by high-throughput sequencing technology. The results showed that the repeated application of modified biochar significantly reduced cadmium and lead in wheat grains and the available heavy metal in soil. The cadmium content in wheat grains decreased by 21%~35%， and lead content in grains declined by 27%~39%. For available cadmium and lead in soil， the contents decreased by 21%~43% and 15%~28%， respectively. The application of modified biochar could significantly reduce abundance of the fungivorous nematode Aphelenchoides， and the proportion of plant-parasitic soil nematodes Merlinius and Paratylenchus both increased to over 40%. The proportion of the c-p2 nematode group in soil decreased，while the c-p3 group increased. The free living nematode maturity index （MI） decreased， but the plant parasitic nematode maturity index （PPI） increased. It was indicated that the response of soil nematodes from different trophic groups or life histories to heavy metal stress were different. Overall， the community of nematodes among the different modified biomass treatments was relatively similar. The cadmium content in wheat grains could reach the minimum after 4 years of repeated application of modified biochar， and the lead content in wheat grains after 2 year biochar application could be lower than the limit of 0.2 mg·kg^-1 in GB 2762—2017. The application of modified biochar could affect the soil environment through the change of the nematode community structure， and it had strong application value in comprehensive utilization and remediation of heavy metal contaminated soil.

Key words: modified biochar, heavy metals in soil, soil nematodes, high throughput sequencing

摘要：

为研究连续施用改性生物质炭对农田镉铅污染土壤修复及线虫群落的影响，以改性生物质炭为材料，开展连施改性生物质炭2、3、4和5年的定位试验。通过测定小麦籽粒和土壤有效态镉铅含量，探究改性生物质炭持续钝化土壤镉铅的能力，并利用高通量测序技术，探究改性生物质炭对土壤线虫群落的影响。结果表明，改性生物质炭能显著降低小麦籽粒和土壤有效态镉铅含量，其中，小麦籽粒镉含量降幅为21%~35%，小麦籽粒铅含量降幅为27%~39%；土壤有效态镉铅含量降幅分别为21%~43%和15%~28%。施用改性生物质炭降低食真菌土壤线虫滑刃属（Aphelenchoides）丰度，而植物寄生性土壤线虫默林属（Merlinius）、针属（Paratylenchus）等占比均增至40%以上。土壤中线虫c-p2类群的比例大幅降低，而c-p3类群占比增加；自由生活线虫成熟指数减少，植物寄生类线虫成熟指数增加，表明不同营养类群、不同生活史的土壤线虫对重金属污染的反应特性不同。整体而言，连施不同年限处理间线虫群落变化不明显。以上表明，小麦籽粒镉含量在连续施用改性生物质炭4年达到最低，小麦籽粒铅含量施用改性生物质炭2年即可低于GB 2762—2017限值0.2 mg·kg^-1。施入改性生物质炭可调节线虫群落结构，从而影响土壤环境，在重金属污染土壤综合利用与修复方面具有较强的应用价值。

关键词: 改性生物质炭, 土壤重金属, 土壤线虫, 高通量测序

CLC Number:

S154

Zhenhua MA, Qianru SHI, Xinjie NING, Hongyang WEI, Can WANG, Jingjing ZHANG, Biao ZHANG, Suqin YANG. Effects of Modified Biochar on Soil Nematode Community in Cadmium and Lead Contaminated Soil[J]. Journal of Agricultural Science and Technology, 2025, 27(2): 201-210.

马振华, 时倩茹, 宁欣杰, 魏宏杨, 王璨, 张静静, 张彪, 杨素勤. 生物质炭对镉铅污染土壤线虫群落的影响[J]. 中国农业科技导报, 2025, 27(2): 201-210.

Figures/Tables 7

References 47

1	黄卫, 庄荣浩, 刘辉, 等. 农田土壤镉污染现状与治理方法研究进展[J]. 湖南师范大学自然科学学报, 2022, 45(1): 49-56.
	HUANG W, ZHUANG R H, LIU H, et al.. Recent advances of the current situation and remediation methods of cadmium contamination in paddy soil [J]. J. Nat. Sci. Hunan Norm. Univ., 2022, 45(1): 49-56.
2	杨莉, 付婧, 文子伟, 等. 6种低温生物质炭的制备及结构表征[J]. 吉林农业大学学报, 2021, 43(5): 565-573.
	YANG L, FU J, WEN Z W, et al.. Preparation and structure characterization of six kinds of low temperature biochar [J]. J. Jilin Agric. Univ., 2021, 43(5): 565-573.
3	孙宇龙, 张永利, 苏有健, 等. 生物质炭对土壤物理结构性状和水分特征影响的研究进展[J]. 江苏农业科学, 2022, 50(23): 25-32.
	SUN Y L, ZHANG Y L, SU Y J, et al.. Effects of biochar on soil physical structure properties and hydrologic properties: a review [J]. Jiangsu Agric. Sci., 2022, 50(23): 25-32.
4	杨文浩, 李佩, 周碧青, 等. 生物炭缓解污染土壤中植物的重金属胁迫研究进展[J]. 福建农林大学学报(自然科学版), 2019, 48(6): 695-705.
	YANG W H, LI P, ZHOU B Q, et al.. Biochar-mediated alleviation of heavy metal stress in plants growing in contaminated soils: a review [J]. J. Fujian Agric. For. Univ.(Nat. Sci.), 2019, 48(6): 695-705.
5	左静, 陈德, 郭虎, 等. 小麦秸秆生物质炭对旱地土壤铅镉有效性及小麦、玉米吸收的影响[J]. 农业环境科学学报, 2017, 36(6): 1133-1140.
	ZUO J, CHEN D, GUO H, et al.. Effects of biochar on Cd Pb availability and uptake by maize and wheat in upland soil [J]. J. Agric. Environ. Sci., 2017, 36(6): 1133-1140.
6	施琪, 鲁然英, 常德政, 等. 增施生物质炭对镉污染土壤的修复效果研究[J]. 江西农业学报, 2019, 31(10): 83-87.
	SHI Q, LU R Y, CHANG D Z, et al.. Effects of increasing biochar application on remediation of Cd contaminated soil [J]. Acta Agric. Jiangxi, 2019, 31(10): 83-87.
7	肖和友, 朱伟, 王海军, 等. 连续施用不同生物质炭对植烟土壤特性和烤烟品质的影响[J]. 中国烟草科学, 2021, 42(3): 19-25.
	XIAO H Y, ZHU W, WANG H J, et al.. Effects of continuous application of different biochar on tobacco-planting soil, flue-cured tobacco leaf quality [J]. China Tob. Sci., 2021, 42(3): 19-25.
8	杨素勤, 魏森, 张彪, 等. 连续施用改性生物质炭对镉铅土壤修复效果及其对微生物群落结构的影响[J]. 农业环境科学学报, 2022, 41(7): 1460-1471.
	YANG S Q, WEI S, ZHANG B, et al.. Remediation effect of continuous application of modified biochar on cadmium-and Lead-contaminated soil and its effect on microbial community structure [J]. J. Agro-Environ. Sci., 2022, 41(7): 1460-1471.
9	杜晓芳, 李英滨, 刘芳, 等. 土壤微食物网结构与生态功能[J]. 应用生态学报, 2018, 29(2): 403-411.
	DU X F, LI Y B, LIU F, et al.. Structure and ecological function of soil micro-food web [J]. Acta Appl. Ecol., 2018, 29(2): 403-411.
10	LU Q F, LIU T T, WANG N Q, et al.. A review of soil nematodes as biological indicators for the assessment of soil health [J]. Front. Agric. Sci. Eng., 2020, 7(3): 275-281.
11	ZHANG X K, LI Q, LIANG W J, et al.. Soil nematode response to biochar addition in a Chinese wheat field [J]. Pedosphere, 2013, 23(1): 98-103.
12	杨贝贝, 朱新萍, 赵一, 等. 生物炭基肥施用对棉田土壤线虫群落结构的影响[J]. 中国土壤与肥料, 2020(4): 66-71.
	YANG B B, ZHU X P, ZHAO Y, et al.. Effect of biochar based application on soil nematode community structure in cotton field [J]. Soil Fert. Sci. China, 2020(4): 66-71.
13	牛亚茹. 施用生物质炭对日光温室黄瓜生长及土壤微生物和线虫群落结构的影响[D]. 南京: 南京农业大学, 2016.
	NIU Y R. Effect of biochar on cucumber growth and soil microbial and nematode community structure in sunlight greenhouse [D]. Nanjing: Nanjing Agricultural University, 2016.
14	DOMENE X, MATTANA S, SÁNCHEZ-MORENO S. Biochar addition rate determines contrasting shifts in soil nematode trophic groups in outdoor mesocosms: an appraisal of underlying mechanisms [J/OL]. Appl. Soil Ecol., 2021, 158: 103788 [2023-03-23]. .
15	RENČO M, ČEREVKOVÁ A, HLAVA J, et al.. Life in a contaminated environment: how soil nematodes can indicate long-term heavy-metal pollution [J/OL]. J. Nematol., 2022, 54(1): 20220053 [2023-03-23]. .
16	邓普荣, 姚志, 刘勇波. 基于线虫生物多样性的稻田镉污染土壤修复成效研究[J]. 环境科学研究, 2022, 35(10): 2378-2387.
	DENG P R, YAO Z, LIU Y B. Effects of cadmium contamination remediation on the biodiversity of soil nematodes in paddy fields [J]. Res. Environ. Sci., 2022, 35(10): 2378-2387.
17	中华人民共和国生态环境部, 国家市场监督管理总局. 土壤环境质量农用地土壤污染风险管控标准(试行): [S]. 北京: 中国标准出版社, 2018.
18	鲍士旦. 土壤农化分析[M]. 第三版. 北京: 中国农业出版社, 2000: 163-165.
19	刘凤枝,刘铭,蔡彦明,等. 土壤质量有效态铅和镉的测定原子吸收法: [S]. 北京: 中国标准出版社, 2009.
20	中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准食品中镉的测定: [S]. 北京: 中国标准出版社, 2014.
21	李琪, 梁文举, 姜勇. 农田土壤线虫多样性研究现状及展望[J]. 生物多样性, 2007, 15(2): 134-141.
	LI Q, LIANG W J, JIANG Y. Present situation and prospect of soil nematode diversity in farmland ecosystems [J]. Biodiversity Sci., 2007,15(2): 134-141.
22	YEATES G W, BONGERS T, DE GOEDE R G M, et al.. Feeding habits in nematode families and genera:an outline for soil ecologists [J]. J. Nematol., 1993, 25(3): 315-331.
23	BONGERS T. The maturity index:an ecological measure of environmental disturbance based on nematode species composition [J]. Oecologia, 1990, 83(1): 4-19.
24	中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局. 食品安全国家标准食品中污染物限量: [S]. 北京: 中国标准出版社, 2017.
25	汪鹏, 王静, 陈宏坪, 等. 我国稻田系统镉污染风险与阻控[J]. 农业环境科学学报, 2018, 37(7): 1409-1417.
	WANG P, WANG J, CHEN H P, et al.. Cadmium risk and mitigation in paddy systems in China [J]. J. Agro-Environ. Sci., 2018, 37(7): 1409-1417.
26	ZHAO F J, WANG P. Arsenic and cadmium accumulation in rice and mitigation strategies [J]. Plant Soil, 2020, 446(1): 1-21.
27	CLEMENS S, AARTS M G M, THOMINE S, et al.. Plant science: the key to preventing slow cadmium poisoning [J].Trends Plant Sci.,2013, 18(2): 92-99.
28	周志云. 磷酸改性生物炭和氯化物混施对小麦吸收铅镉的影响[D]. 郑州: 河南农业大学, 2018.
	ZHOU Z Y. Effects of phosphoric-acid modified biochar combined with chlorine on lead and cadmium absorption in wheat [D]. Zhengzhou: Henan Agricultural University, 2018.
29	景鑫鑫. 几种钝化剂修复铅镉污染石灰性土壤的效果研究[D]. 郑州: 河南农业大学, 2015.
	JING X X. Effects of different stabilizers on the remediation for heavy metal contaminated alkaline soil [D]. Zhengzhou: Henan Agricultural University, 2015.
30	付煜恒, 张惠灵, 王宇, 等. 磷酸盐对铅镉复合污染土壤的钝化修复研究[J]. 环境工程, 2017, 35(9): 176-180.
	FU Y H, ZHANG H L, WANG Y, et al.. Immobilization of soil contaminated by lead and cadmium using phosphate [J]. Environ. Eng., 2017, 35(9): 176-180.
31	孙新, 李琪, 姚海凤, 等. 土壤动物与土壤健康[J]. 土壤学报, 2021, 58(5): 1073-1083.
	SUN X, LI Q, YAO H F, et al.. Soil fauna and soil health [J]. Acta Pedol. Sin., 2021, 58(5): 1073-1083.
32	张晓珂, 梁文举, 李琪. 我国土壤线虫生态学研究进展和展望[J]. 生物多样性, 2018, 26(10): 1060-1073.
	ZHANG X K, LIANG W J, LI Q. Recent progress and future directions of soil nematode ecology in China [J]. Biodiversity Sci., 2018, 26 (10): 1060-1073.
33	NEHER D A. Role of nematodes in soil health and their use as indicators [J]. J. Nematol., 2001, 33(4): 161-168.
34	HAN D C, ZHANG X K, TOMAR V V S, et al.. Effects of heavy metal pollution of highway origin on soil nematode guilds in North Shenyang, China [J]. J. Environ. Sci., 2009, 21(2): 193-198.
35	LI H X, HU F. Effect of bacterial-feeding nematode inoculation on wheat growth and N and P uptake [J]. Pedosphere, 2001, 11(1): 57-62.
36	XIAO H F, GRIFFITHS B, CHEN X Y, et al.. Influence of bacterial-feeding nematodes on nitrification and the ammonia-oxidizing bacteria (AOB) community composition [J]. Appl. Soil Ecol., 2010, 45(3): 131-137.
37	王赢利, 王宏洪, 廖金铃, 等. 电子垃圾拆解地重金属污染对稻田土壤线虫群落结构的影响[J]. 农业环境科学学报, 2015, 34(5): 874-881.
	WANG Y L, WANG H H, LIAO J L, et al.. Effects of heavy metal contamination on nematode communities in paddy soils of an e-waste recycling area [J]. J. Agro-Environ. Sci., 2015, 34(5): 874-881.
38	成碧君, 刘良坡, 张红梅, 等. 重金属对煤矿区土壤线虫群落结构的影响[J]. 山西农业科学, 2022, 50(12): 1680-1688.
	CHENG B J, LIU L P, ZHANG H M, et al.. Effects of heavy metals on soil nematode community structure in coal mine areas [J]. Shanxi Agric. Sci., 2022, 50(12): 1680-1688.
39	吕莹. 线虫群落对大连滨海石油与土壤重金属污染及绿地生态系统恢复的指示作用研究[D]. 大连: 辽宁师范大学, 2012.
	LYU Y. The study of responses of nematode communites to crude oil contamination, soil heavy metal pollution and recovery of urban greenland in Dalian [D]. Dalian: Liaoning Normal University, 2012.
40	NAGY P, BAKONYI G, BONGERS T, et al.. Effects of microelements on soil nematode assemblages seven years after contaminating an agricultural field [J]. Sci. Total Environ., 2003, 320(2): 131-143.
41	ZHANG W D, XIAO Y, WANG X F, et al.. Soil nematode community characteristics around the Gangue hill of Fushun West Open-pit mine [J]. Helminthologia, 2011, 48(2): 116-123.
42	DOROSZUK A. Populations under stress:analysis on the interface between ecology and evolutionary genetics in nematodes [D]. Wageningen: Wageningen University, 2007.
43	李孟洁. 小麦-玉米轮作体系中长期施肥对土壤线虫群落结构的影响[D]. 郑州: 郑州大学, 2018.
	LI M J. Effects of long-term fertilization on soil nematode community structure in wheat-maize rotation system [D]. Zhengzhou: Zhengzhou University, 2018.
44	杨盼盼,黄菁华,张欣玥,等.长期施肥对渭北旱塬麦田土壤线虫群落特征的影响[J]. 干旱地区农业研究, 2022, 40(5): 242-251.
	YANG P P, HUANG J H, ZHANG X Y, et al.. Effects of long-term fertilization on soil nematode community of wheat field in Weibei dryland, China [J]. Agric. Res. Arid Areas, 2022, 40(5):242-251.
45	金娜, 刘倩, 简恒. 植物寄生线虫生物防治研究新进展[J]. 中国生物防治学报, 2015, 31(5): 789-800.
	JIN N, LIU Q, JIAN H. Advances on biological control of plant-parasitic nematodes [J]. Chin. J. Biol. Control, 2015, 31(5): 789-800.
46	段玉玺. 植物线虫学[M]. 北京: 科学出版社, 2011: 52-53.
47	JORÉ A R M， CARMEN G， MIGUEL E， et al.. Effect of mine tailing on the spatial variability of soil nematodes from lead pollution in La Union （Spain） [J]. Sci. Total Environ.， 2014， 473-474：518-529.

营养类型 Nutritional type	属名 Genus name	c-p值 c-p value	比例Proportion/%
营养类型 Nutritional type	属名 Genus name	c-p值 c-p value	CK	Y_15-16	Y_15-17	Y_15-18	Y_15-19
食细菌线虫 Ba	丽突属Acrobeles	2	0.03	0.11	0.00	0.01	0.03
	拟丽突属Acrobeloides	2	9.69	13.86	8.46	5.36	10.99
	无咽属Alaimus	4	0.00	0.01	0.01	0.01	0.00
	头叶属Cephalobus	2	0.01	0.07	0.00	0.00	0.00
	真头叶属Eucephalobus	2	0.04	0.08	0.09	0.00	0.05
	真单宫属Eumonhystera	2	0.02	0.02	0.01	0.01	0.00
	Oscheius	1	0.61	0.06	0.01	0.05	0.00
	盆咽属Panagrolaimus	1	0.05	0.01	0.58	0.01	0.02
	绕线属Plectus	2	0.00	0.00	0.00	0.00	0.00
	棱咽属Prismatolaimus	3	0.22	0.08	0.10	0.05	0.05
食真菌线虫Fu	滑刃属Aphelenchoides	2	72.54	10.31	0.23	2.08	6.34
	茎属Ditylenchus	2	0.07	0.15	0.17	0.01	0.56
	丝尾垫刃属Filenchus	2	0.00	0.00	0.00	0.00	0.00
	细齿属Leptonchus	4	0.00	0.00	0.01	0.00	0.00
捕食/杂食性线虫Om	Allodorylaimus	4	0.00	0.05	0.03	0.03	0.02
	孔咽属Aporcelaimellus	5	2.37	5.28	5.60	5.47	5.74
	狭咽属Discolaimium	5	0.14	0.00	0.00	0.00	0.00
	盘咽属Discolaimus	5	0.00	0.00	0.02	0.00	0.00
	锯齿属Prionchulus	4	0.01	0.00	0.00	0.03	0.00
	Trischistoma	3	0.01	0.00	0.00	0.00	0.00
植物寄生性线虫Pp	刺属Belonolaimus	5	0.01	0.00	0.00	0.00	0.01
	环属Criconema	3	0.00	0.00	0.00	0.00	0.00
	居中属Geocenamus	3	0.00	0.00	0.01	0.01	0.01
	螺旋属Helicotylenchus	3	0.20	0.02	0.04	0.00	0.03
	Irantylenchus	2	0.04	0.06	0.01	0.02	0.01
	默林属Merlinius	3	2.93	37.79	43.45	42.67	37.86
	针属Paratylenchus	2	2.02	26.48	33.34	41.88	34.70
	短体属Pratylenchus	3	0.06	0.10	0.08	0.02	0.06
	Sakia	2	0.01	0.00	0.01	0.00	0.00
	矮化属Tylenchorhynchus	3	0.25	0.01	0.62	0.00	0.02

营养类型 Nutritional type	属名 Genus name	c-p值 c-p value	比例Proportion/%
营养类型 Nutritional type	属名 Genus name	c-p值 c-p value	CK	Y_15-16	Y_15-17	Y_15-18	Y_15-19
食细菌线虫 Ba	丽突属Acrobeles	2	0.03	0.11	0.00	0.01	0.03
	拟丽突属Acrobeloides	2	9.69	13.86	8.46	5.36	10.99
	无咽属Alaimus	4	0.00	0.01	0.01	0.01	0.00
	头叶属Cephalobus	2	0.01	0.07	0.00	0.00	0.00
	真头叶属Eucephalobus	2	0.04	0.08	0.09	0.00	0.05
	真单宫属Eumonhystera	2	0.02	0.02	0.01	0.01	0.00
	Oscheius	1	0.61	0.06	0.01	0.05	0.00
	盆咽属Panagrolaimus	1	0.05	0.01	0.58	0.01	0.02
	绕线属Plectus	2	0.00	0.00	0.00	0.00	0.00
	棱咽属Prismatolaimus	3	0.22	0.08	0.10	0.05	0.05
食真菌线虫Fu	滑刃属Aphelenchoides	2	72.54	10.31	0.23	2.08	6.34
	茎属Ditylenchus	2	0.07	0.15	0.17	0.01	0.56
	丝尾垫刃属Filenchus	2	0.00	0.00	0.00	0.00	0.00
	细齿属Leptonchus	4	0.00	0.00	0.01	0.00	0.00
捕食/杂食性线虫Om	Allodorylaimus	4	0.00	0.05	0.03	0.03	0.02
	孔咽属Aporcelaimellus	5	2.37	5.28	5.60	5.47	5.74
	狭咽属Discolaimium	5	0.14	0.00	0.00	0.00	0.00
	盘咽属Discolaimus	5	0.00	0.00	0.02	0.00	0.00
	锯齿属Prionchulus	4	0.01	0.00	0.00	0.03	0.00
	Trischistoma	3	0.01	0.00	0.00	0.00	0.00
植物寄生性线虫Pp	刺属Belonolaimus	5	0.01	0.00	0.00	0.00	0.01
	环属Criconema	3	0.00	0.00	0.00	0.00	0.00
	居中属Geocenamus	3	0.00	0.00	0.01	0.01	0.01
	螺旋属Helicotylenchus	3	0.20	0.02	0.04	0.00	0.03
	Irantylenchus	2	0.04	0.06	0.01	0.02	0.01
	默林属Merlinius	3	2.93	37.79	43.45	42.67	37.86
	针属Paratylenchus	2	2.02	26.48	33.34	41.88	34.70
	短体属Pratylenchus	3	0.06	0.10	0.08	0.02	0.06
	Sakia	2	0.01	0.00	0.01	0.00	0.00
	矮化属Tylenchorhynchus	3	0.25	0.01	0.62	0.00	0.02

处理 Treatment	丰富度指数Abundance index		均匀度指数 Evenness index		多样性指数 Diversity index		自由生活线虫成熟指数MI	植物寄生性线虫成熟指数 PPI
处理 Treatment	Chao指数Chao index	Ace指数 Ace index	Simpsoneven指数Simpsoneven index	Shannoneven指数Shannoneven index	Simpson指数Simpson index	Shannon指数Shannon index	自由生活线虫成熟指数MI	植物寄生性线虫成熟指数 PPI
CK	22.8 a	24.0 a	0.085 b	0.34 b	0.55 a	1.06 b	1.79 a	0.14 b
Y_15-16	22.1 a	22.8 a	0.177 a	0.51 a	0.27 b	1.55 a	0.76 b	1.67 a
Y_15-17	21.5 a	23.6 a	0.149 a	0.43 ab	0.36 b	1.29 ab	0.47 b	1.99 a
Y_15-18	22.2 a	22.4 a	0.141 ab	0.41 ab	0.38 b	1.21 ab	0.43 b	2.12 a
Y_15-19	19.7 a	20.5 a	0.172 a	0.48 a	0.31 b	1.43 ab	0.65 b	1.83 a

处理 Treatment	丰富度指数Abundance index		均匀度指数 Evenness index		多样性指数 Diversity index		自由生活线虫成熟指数MI	植物寄生性线虫成熟指数 PPI
处理 Treatment	Chao指数Chao index	Ace指数 Ace index	Simpsoneven指数Simpsoneven index	Shannoneven指数Shannoneven index	Simpson指数Simpson index	Shannon指数Shannon index	自由生活线虫成熟指数MI	植物寄生性线虫成熟指数 PPI
CK	22.8 a	24.0 a	0.085 b	0.34 b	0.55 a	1.06 b	1.79 a	0.14 b
Y_15-16	22.1 a	22.8 a	0.177 a	0.51 a	0.27 b	1.55 a	0.76 b	1.67 a
Y_15-17	21.5 a	23.6 a	0.149 a	0.43 ab	0.36 b	1.29 ab	0.47 b	1.99 a
Y_15-18	22.2 a	22.4 a	0.141 ab	0.41 ab	0.38 b	1.21 ab	0.43 b	2.12 a
Y_15-19	19.7 a	20.5 a	0.172 a	0.48 a	0.31 b	1.43 ab	0.65 b	1.83 a

[1]	Danyi SHI, Yu QIU, Chengzhen HUANG, Juan WANG. Effect of Acid Modified Biochar on Infiltration Characteristics of Coastal Saline Soil [J]. Journal of Agricultural Science and Technology, 2024, 26(9): 183-192.
[2]	Shegang SHAO, Ting LI, Yong LIU, Lanwen LIN, Dong ZHANG, Dong NI, Junjie LI, Li’an ZHU. Effects of Exogenous Promoting Bacteria Agent on Decomposition Characteristics and Microbial Community Structure of Rice Straw [J]. Journal of Agricultural Science and Technology, 2023, 25(9): 166-177.
[3]	SU Yumeng§, ZHANG Xuting§, Terigele, TIAN Min, SHANG Xiaorui, LI Guojing, WANG Ruigang*. Identification of microRNAs in Caragana intermedia Kuang by High Throughput Sequencing Under Drought Stress [J]. Journal of Agricultural Science and Technology, 2021, 23(3): 51-57.
[4]	MA Yanru1,2, MENG Haibo2, SHEN Yujun2, DING Jingtao2, WANG Liming1*. Research on Adsorption Effect of Ammonia-nitrogen from Biogas Slurry by Modified Biochar [J]. Journal of Agricultural Science and Technology, 2018, 20(11): 135-144.