Strategy and Application for Constructing Tobacco Mutant Library

doi:10.3969/j.issn.1008-0864.2010.06.06

›› 2010, Vol. 12 ›› Issue (6): 28-35.DOI: 10.3969/j.issn.1008-0864.2010.06.06

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Strategy and Application for Constructing Tobacco Mutant Library

LIU Xiao-bei1, WU Geng1, ZHANG Qian2, LIU Guan-shan3, LU Tie-gang2, WANG Yuan-ying3

(1.Capital Normal University, Beijing 100048|2.Biotechnology Research Institute, National Key Facility |for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081; |3.Tobacco Research Institute, CAAS, Shandong Qingdao 266101, China)

Received:2010-04-29 Revised:2010-06-03 Online:2010-12-15 Published:2010-09-19

烟草突变体库的创建策略及其应用

刘晓蓓1,吴赓1,张芊2,刘贯山3,路铁刚2,王元英3

(1.首都师范大学, 北京 100048|2.中国农业科学院生物技术研究所, 国家基因资源与遗传改良重大科学工程, 北京 100081|3.中国农业科学院烟草研究所, 山东青岛 266101)

通讯作者: 路铁刚,研究员,博士,研究方向为植物功能基因组学、植物生物技术。E-mail:tiegang@caas.net.cn。王元英,研究员,博士,研究方向为烟草遗传育种学、生物技术。E-mail:wyytob@126.com
作者简介:刘晓蓓,硕士研究生,主要从事植物功能基因组学研究。E-mail:casablanca_007lx@163.com。吴赓,硕士研究生,主要从事植物生物技术研究。E-mail:mamaolu@163.com。刘晓蓓与吴赓为本文共同第一作者。
基金资助:
国家烟草专卖局重点科技项目(110200701022)资助。

Abstract

Abstract:

Tobacco (Nicotiana tabacum) is one of the most important cash crops in the world and it is also a model plant for biotechnology research. At present, tobacco mutants has been widely used in functional genomics study and breeding, which will be further promoted by constructing an efficient and large-scale tobacco mutant library. This paper reviews the applications of chemical mutagenesis, ion radiation-induced mutagenesis and insertion mutagenesis in mutant library construction. In addition, considering the character of tobacco genome, new strategies of tobacco mutant library construction were described in combination with new methods for functional genomics study, including whole genome overexpression strategy based on Gateway technology and EST-RNA interference strategy based on RMHR technology. How to utilize them to study tobacco functional genome is also discussed in this paper.

Key words: tobacco, mutant library, functional genomics, Gateway technology, RMHR (rolling-circle amplification-mediated hpRNA) technology

摘要：

烟草(Nicotiana tabacum)是重要的经济作物及研究植物生命活动的模式植物。目前烟草突变体已广泛应用于烟草功能基因组研究及遗传育种,大规模高效烟草突变体库的创建将进一步推动烟草功能基因组研究,加快新品种的培育。综述了化学诱变、电离辐射和插入标签法在烟草突变体库创建中的应用;此外,针对烟草基因组的特点,结合近年来一些植物功能基因组研究的新技术、新方法,提出了烟草突变体库创建的新策略,即基于Gateway技术的烟草全基因组过量表达策略和基于RMHR技术的烟草EST-RNA干涉策略,并就如何应用这些突变体库进行烟草功能基因组研究进行了探讨。

关键词: 烟草;突变体库;功能基因组;Gateway技术;RMHR(rolling-circle amplification-mediated hpRNA)技术

CLC Number:

S572.035.3

LIU Xiao-bei1, WU Geng1, ZHANG Qian2, LIU Guan-shan3, LU Tie-gang2, WANG Yuan-yi. Strategy and Application for Constructing Tobacco Mutant Library[J]. , 2010, 12(6): 28-35.

刘晓蓓1,吴赓1,张芊2,刘贯山3,路铁刚2,王元英3. 烟草突变体库的创建策略及其应用[J]. , 2010, 12(6): 28-35.

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Strategy and Application for Constructing Tobacco Mutant Library

烟草突变体库的创建策略及其应用

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