Effects of 60Co-&gamma|Ray Irradiation on Antioxidant Enzyme Activities in Tobacco

doi:10.3969/j.issn.1008-0864.2012.01.10

›› 2012, Vol. 14 ›› Issue (1): 72-75.DOI: 10.3969/j.issn.1008-0864.2012.01.10

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Effects of 60Co-&gamma|Ray Irradiation on Antioxidant Enzyme Activities in Tobacco

DU Shi-zhang1, Liu Ting-ting2, FENG Bin1| TIAN Xia2, GONG Yuan-ya2, SUN Ying-kun2, WANG Jin3

(1.Mianyang Normal College, Sichuan Mianyang 621000|2.Plant Biotechnology Research Centre, Southwest University of Science and Technology, Sichuan Mianyang 621000|3.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2011-06-24 Revised:2011-07-20 Online:2012-02-15 Published:2011-12-15

辐照对烟草抗氧化酶活性的影响

杜世章1,刘婷婷2,代其林2,奉斌2,杨娟2,谢琳2,鲜先毅2,王劲3

(1.绵阳师范学院, 四川绵阳 621000|2.西南科技大学植物生物技术研究中心, 四川绵阳 621000|3.中国农业科学院生物技术研究所, 北京 100081)

通讯作者: 王劲,教授,主要从事分子生物学方面研究。E-mail:wjdsz@vip.sina.com
作者简介:杜世章,副教授,博士,主要从事分子生物学方面的研究。
基金资助:
转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅青年基金项目(09ZA034);西南科技大学博士研究基金(11zx7104)资助。

Abstract

Abstract:

Effects of 300Gy 60Co-γ ray irradiation on the activities of 3 antioxidant enzymes in tobacco were studied in this paper. The results showed that the activities of superoxide dismutase (SOD), peroxidas (POD) and catalse (CAT) in tobacco were gradually increased. All the activities of SOD POD and CAT reached the maximums at 12 h and then gradually decreased. Similar results were also observed in the expression of sod, pod and cat genes. These results indicated that the activities of antioxidant enzymes could play an important role in tobacco tolerance against irradiation.

Key words: tobacco, 60 Co-γ, antioxidant enzymes

摘要：

以K326烟草植株为材料,研究了60Co-γ射线辐照对烟草叶片抗氧化酶活性及其基因转录水平的影响。结果表明,经300Gy 60Co-γ射线辐照后0~48 h期间,烟草叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)三种抗氧酶活性以及其相应酶基因转录水平都逐渐提高,SOD、POD和CAT酶活性及其基因转录水平在12 h均达最大值,三者的活性及其基因转录水平随后都逐渐降低。我们的实验结果表明烟草叶片的抗氧化酶活性在其耐辐射能力起了重要作用。

关键词: 烟草;60Co-γ;抗氧化酶

CLC Number:

Q945

DU Shi-zhang1, Liu Ting-ting2, FENG Bin1| TIAN Xia2, GONG Yuan-ya2, SUN Ying-ku. Effects of 60Co-&gamma|Ray Irradiation on Antioxidant Enzyme Activities in Tobacco[J]. , 2012, 14(1): 72-75.

杜世章1,刘婷婷2,代其林2,奉斌2,杨娟2,谢琳2,鲜先毅2,王劲3. 辐照对烟草抗氧化酶活性的影响[J]. , 2012, 14(1): 72-75.

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Effects of 60Co-&gamma|Ray Irradiation on Antioxidant Enzyme Activities in Tobacco

辐照对烟草抗氧化酶活性的影响

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