A New Rapid Method for Estimating Rice Seed Alkaline Lipase and its Application

›› 2009, Vol. 11 ›› Issue (S2): 43-45.

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A New Rapid Method for Estimating Rice Seed Alkaline Lipase and its Application

ZHANG Ying1,2, WU Yue-jin1, HE Cheng-fang1, LIU Jie1, JIANG Jia-yue1

(1.Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031|2.Anhui Academy of Agricultural Sciences, Institute of Rice Research, Key Laboratory of Rice Genetics &|Breeding of Anhui Province, |Hefei |230031, China)

Received:2009-08-04 Revised:2009-11-04 Online:2009-12-31 Published:2009-12-31

一种新的水稻种子碱性脂肪酶活性快速检测方法及其应用

张瑛1,2,吴跃进1,何成芳1,刘洁1,蒋家月1

(1.中国科学院等离子体物理研究所,安徽省离子束生物工程学重点实验室, 合肥 230031|2.安徽省农业科学院水稻研究所, 安徽省水稻遗传育种重点实验室, 合肥230031)

作者简介:张瑛,副研究员,博士,主要从事植物生理生化研究。E-mail:zhangying56888@sina.com
基金资助:
“十一五”国家科技支撑计划资助项目(2006BAD01A01-5);安徽省优秀青年基金项目资助。

Abstract

Abstract:

It is extremely widespread about the research on microbial alkaline lipase and its application, but the rice seed alkaline lipase are poorly understood. The paper introduced a new plate examination method for estimating rice seed alkaline lipase activity. Using this method, rice seed materials with different alkaline lipase activity was screened, and used to study the function on alkaline lipase activity in an accelerated aging experiment. The result indicated that the method for eatimating alkaline lipase and its application in grain artificial aging are of great value to characters of grain storage.

Key words: rice, seed, alkaline lipase, plate examination method, accelerated aging

摘要：

微生物碱性脂肪酶研究及其应用均非常广泛,而关于水稻种子碱性脂肪酶却知之甚少。介绍一种新的水稻种子碱性脂肪酶活性平板检测方法,并利用该方法筛选碱性脂肪酶活性高低不同的材料以研究碱性脂肪酶在稻谷加速老化过程中的作用。结果表明碱性脂肪酶检测方法及其在稻谷人工老化过程中的作用对揭示稻谷储藏特性具有重要研究价值。

关键词: 水稻;种子;碱性脂肪酶;检测方法;加速老化

CLC Number:

S339.3

ZHANG Ying1,2, WU Yue-jin1, HE Cheng-fang1, LIU Jie1, JIANG Jia-yue1. A New Rapid Method for Estimating Rice Seed Alkaline Lipase and its Application[J]. , 2009, 11(S2): 43-45.

张瑛1,2,吴跃进1,何成芳1,刘洁1,蒋家月1. 一种新的水稻种子碱性脂肪酶活性快速检测方法及其应用[J]. , 2009, 11(S2): 43-45.

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A New Rapid Method for Estimating Rice Seed Alkaline Lipase and its Application

一种新的水稻种子碱性脂肪酶活性快速检测方法及其应用

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