Microbial Community Structure of Potato Rhizosphere Soil and Its Response to Drought Stress

doi:10.13304/j.nykjdb.2021.0692

Journal of Agricultural Science and Technology ›› 2022, Vol. 24 ›› Issue (6): 58-69.DOI: 10.13304/j.nykjdb.2021.0692

• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles

Microbial Community Structure of Potato Rhizosphere Soil and Its Response to Drought Stress

Lili WANG¹^,⁵(), Congpei YIN²(), Feng LI³, Zhimin YANG³, Fangming LIU¹, Baisong LIN¹, Xiaojing LIU¹, Haijun LIU⁴, Jing SUN⁴, Dongdong SHAN⁴, Jianghui CUI²^,⁵(), Zhenqing ZHANG⁴^,⁵()

^1.Weichang Manchu and Mongolian Autonomous County Potato Research Institute，Hebei Chengde 068450，China
^2.College of Agronomy，Hebei Agricultural University，Hebei Baoding 071000，China
^3.Zhangjiakou Academy of Agricultural Sciences，Hebei Zhangjiakou 075000，China
^4.Weichang Manchu and Mongolian Autonomous County Potato Industry Service Center，Hebei Chengde 068450，China
^5.Hebei Seed Potato Industry Technology Research Institute，Hebei Chengde 068450，China

Received:2021-08-12 Accepted:2021-11-15 Online:2022-06-15 Published:2022-06-21
Contact: Jianghui CUI,Zhenqing ZHANG

马铃薯根际土壤细菌群落结构及其对干旱胁迫的响应

王莉莉¹^,⁵(), 殷丛培²(), 李峰³, 杨志敏³, 刘芳明¹, 林柏松¹, 刘晓静¹, 刘海军⁴, 孙靖⁴, 单东东⁴, 崔江慧²^,⁵(), 张振清⁴^,⁵()

^1.围场满族蒙古族自治县马铃薯研究所, 河北承德 068450
^2.河北农业大学农学院, 河北保定 071000
^3.张家口市农业科学院, 河北张家口 075000
^4.围场满族蒙古族自治县马铃薯产业服务中心, 河北承德 068450
^5.河北省马铃薯种薯产业技术研究院, 河北承德 068450

通讯作者: 崔江慧,张振清
作者简介:王莉莉 E-mail：546156736@qq.com
殷丛培 E-mail： ycongpei2015@163.com第一联系人：王莉莉和殷丛培为共同第一作者。
基金资助:
河北省（承德）马铃薯种薯产业技术研究院项目(SC2021028);承德国家可持续发展议程示范区建设科技专项计划项目(202104F014)

Abstract

Abstract:

To study the microbial community structure of potato rhizosphere soil and its response to drought stress， the pot experiment was performed， and the rhizosphere soils of potatoes with different drought stress were as materials. The potatoes rhizosphere soil （planted soil） and CK soil were collected at flowering stages. Then the microbial DNA was isolated， and the V3~V4 region of bacteria was deep sequenced by Illumina Miseq. The characteristics of microbial community structure was analyzed. The results showed that a total of 1 263 889 effective sequences were identified， among which 6 785 OTUs belonged to 33 phyla， 94 classes， 267 orders， 462 families， 919 genera， and 1 930 species. Compared with CK soil before planted potato， Proteobacteria， Actinobacteria， Acidobacteria， Chloroflexi， Bacteroidetes， Firmicutes and Gemmatimonadetes were the dominant phyla in the rhizosphere soil after planted potato， and the relative abundance of Actinobacteria increased significantly. Drought stress increased the relative abundances of Proteobacteria， Bacteroidetes， Gemmatimonadetes and Pseudomonas. Principal coordinates analysis （PCoA） showed that potatoes planted and drought stress were the main causes of variation in soil bacterial community structure. Phylogenetic investigation of communities by reconstruction of unobserved states （PICRUSt） profiling indicated that sequences related to metabolism， signaling transduction， defense mechanism and basic vital activity were enriched in the drought-treated rhizosphere soil， which might be important for plant survival and drought tolerance， and provided new insights for the researches of improving drought tolerance of potatoes by soil microorganisms.

Key words: potato, rhizosphere, microbial community structure, drought stress

摘要：

为研究马铃薯根际土壤细菌的群落结构及其对干旱胁迫的响应，采用盆栽试验，以开花期马铃薯根际土壤为研究对象，提取其总DNA，通过Illumina Miseq对细菌 V3~V4区进行高通量测序，对干旱处理下马铃薯根际微生物的群落结构进行分析。结果表明，共鉴定有效序列1 263 889条，6 785个OTUs归属33个门、94个纲、267个目、462个科、919个属、1 930个种。与对照土壤相比，种植马铃薯后根际土壤中的变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）、绿弯菌门（Chloroflexi）、拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）和芽单胞菌门（Gemmatimonadetes）为优势菌门，其中放线菌门（Proteobacteria）的相对丰度显著增加。干旱处理提高了马铃薯根际土壤中变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、芽单胞菌门（Gemmatimonadetes）和假单胞菌属（Pseudomonas）的相对丰度。PCoA（principal coordinates analysis）分析显示，种植马铃薯和干旱胁迫是造成土壤细菌群落结构变异的重要因素。PICRUSt（phylogenetic investigation of communities by reconstruction of unobserved states）分析表明，干旱处理后根际土壤细菌的变化与马铃薯植株的生理代谢、信号转导、防御机制和基本生命活性等密切相关，对植物的生存和耐旱性具有重要意义，为通过改良土壤微生物环境来提高植物胁迫耐受性提供了重要参考。

关键词: 马铃薯, 根际, 微生物群落结构, 干旱胁迫

CLC Number:

S532

Lili WANG, Congpei YIN, Feng LI, Zhimin YANG, Fangming LIU, Baisong LIN, Xiaojing LIU, Haijun LIU, Jing SUN, Dongdong SHAN, Jianghui CUI, Zhenqing ZHANG. Microbial Community Structure of Potato Rhizosphere Soil and Its Response to Drought Stress[J]. Journal of Agricultural Science and Technology, 2022, 24(6): 58-69.

王莉莉, 殷丛培, 李峰, 杨志敏, 刘芳明, 林柏松, 刘晓静, 刘海军, 孙靖, 单东东, 崔江慧, 张振清. 马铃薯根际土壤细菌群落结构及其对干旱胁迫的响应[J]. 中国农业科技导报, 2022, 24(6): 58-69.

Figures/Tables 10

References 36

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品种Variety	处理Treatment	净光合速率P_n/（µmol·m^-2·s^-1）	蒸腾速率 T_r/（mmol·m^-2·s^-1）	气孔导度 G_s/（mol·m^-2·s^-1）	胞间CO₂浓度 C_i/（µmol·mol^-1）
冀张薯8号 Jizhangshu 8	T0	22.00±2.12 a	2.35±0.33 a	13.83±1.10 a	170.33±10.66 a
	T1	24.80±9.04 a	2.27±0.21 a	10.33±0.74 b	178.53±16.03 a
	T2	17.44±3.10 ab	2.01±0.21 ab	8.50±0.89 bc	182.67±9.18 a
	T3	13.91±1.08 b	1.79±0.05 ab	6.00±0.47 c	195.41±9.50 a
夏波蒂 Shepody	T0	28.01±2.22 a	2.79±0.16 a	13.76±1.35 a	137.42±11.03 b
	T1	18.63±3.66 a	2.17±0.45 b	11.67±1.06 ab	159.00±3.65 ab
	T2	14.73±2.00 b	1.58±0.32 b	8.87±1.51 bc	173.14±7.11 a
	T3	8.97±1.82 c	1.03±0.11 c	4.80±1.08 c	180.00±13.12 a

品种Variety	处理Treatment	净光合速率P_n/（µmol·m^-2·s^-1）	蒸腾速率 T_r/（mmol·m^-2·s^-1）	气孔导度 G_s/（mol·m^-2·s^-1）	胞间CO₂浓度 C_i/（µmol·mol^-1）
冀张薯8号 Jizhangshu 8	T0	22.00±2.12 a	2.35±0.33 a	13.83±1.10 a	170.33±10.66 a
	T1	24.80±9.04 a	2.27±0.21 a	10.33±0.74 b	178.53±16.03 a
	T2	17.44±3.10 ab	2.01±0.21 ab	8.50±0.89 bc	182.67±9.18 a
	T3	13.91±1.08 b	1.79±0.05 ab	6.00±0.47 c	195.41±9.50 a
夏波蒂 Shepody	T0	28.01±2.22 a	2.79±0.16 a	13.76±1.35 a	137.42±11.03 b
	T1	18.63±3.66 a	2.17±0.45 b	11.67±1.06 ab	159.00±3.65 ab
	T2	14.73±2.00 b	1.58±0.32 b	8.87±1.51 bc	173.14±7.11 a
	T3	8.97±1.82 c	1.03±0.11 c	4.80±1.08 c	180.00±13.12 a

品种Variety	处理 Treatment	超氧化物歧化酶活性 SOD activity/（U·min^-1）	过氧化物酶活性 POD activity /（U·min^-1）	过氧化氢酶活性 CAT activity /（U·min^-1）
冀张薯8号 Jizhangshu 8	T0	118.04±8.77 b	73.50±2.45 c	5.38±0.20 b
	T1	123.74±6.62 b	93.76±4.71 b	6.70±0.61 ab
	T2	145.98±7.02 b	98.35±5.03 b	8.01±0.30 a
	T3	240.01±9.22 a	119.98±6.73 a	5.30±0.40 b
夏波蒂 Shepody	T0	44.98±5.66 c	74.03±2.07 c	4.66±0.31 a
	T1	58.06±4.08 b	94.03±3.99 b	4.76±0.58 a
	T2	67.02±5.31 ab	97.77±3.22 b	4.72±0.57 a
	T3	87.96±6.33 a	118.03±4.14 a	3.13±0.71 b

品种Variety	处理 Treatment	超氧化物歧化酶活性 SOD activity/（U·min^-1）	过氧化物酶活性 POD activity /（U·min^-1）	过氧化氢酶活性 CAT activity /（U·min^-1）
冀张薯8号 Jizhangshu 8	T0	118.04±8.77 b	73.50±2.45 c	5.38±0.20 b
	T1	123.74±6.62 b	93.76±4.71 b	6.70±0.61 ab
	T2	145.98±7.02 b	98.35±5.03 b	8.01±0.30 a
	T3	240.01±9.22 a	119.98±6.73 a	5.30±0.40 b
夏波蒂 Shepody	T0	44.98±5.66 c	74.03±2.07 c	4.66±0.31 a
	T1	58.06±4.08 b	94.03±3.99 b	4.76±0.58 a
	T2	67.02±5.31 ab	97.77±3.22 b	4.72±0.57 a
	T3	87.96±6.33 a	118.03±4.14 a	3.13±0.71 b

处理 Treatment	有效序列数 Sequence number	碱基数 Base number	平均长度 Mean length/bp	最短序列长度 Min. length/bp	最长序列长度 Max. length/bp
对照土CK soil	173 462	72 743 138	419.36	257	480
S-T0	150 075	62 852 665	418.84	276	472
S-T1	125 549	52 301 567	416.60	289	455
S-T2	159 305	66 521 294	417.57	243	476
S-T3	178 086	74 359 015	417.53	271	471
JZS-T0	171 403	71 496 233	417.13	214	468
JZS-T1	157 105	65 548 212	417.20	271	474
JZS-T2	161 144	67 378 509	418.09	240	504
JZS-T3	161 222	67 470 566	418.48	235	490

Microbial Community Structure of Potato Rhizosphere Soil and Its Response to Drought Stress

马铃薯根际土壤细菌群落结构及其对干旱胁迫的响应

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处理 Treatment	测序深度 Sequencing depth coverage	丰度指数 Richness index			多样性指数 Diversity index		均匀度指数 Evenness index
处理 Treatment	测序深度 Sequencing depth coverage	Sobs	Chao	ACE	Shannon	Simpson	Shannon	Simpson
对照土CK soil	0.956 3	2 871.00±46.89 a	4 019.46±124.69 a	4 226.19±454.79 a	6.30±0.04 e	0.007±0.001 b	0.791±0.003 e	0.049±0.005 cd
S-T0	0.963 4	3 034.00±126.57 a	4 300.03±193.64 a	4 285.13±178.17 a	6.64±0.04 b	0.004±0.001 bcd	0.828±0.007 b	0.076±0.009 b
S-T1	0.962 7	2 914.33±332.18 a	4 168.09±393.31 a	4 306.80±129.02 a	6.75±0.03 a	0.003±0.000 d	0.846±0.008 a	0.117±0.016 a
S-T2	0.969 5	2 891.67±143.07 a	4 154.81±341.77 a	4 412.31±699.81 a	6.49±0.04 cd	0.004±0.000 bcd	0.813±0.001 bcd	0.078±0.002 b
S-T3	0.972 9	3 093.67±146.73 a	4 318.33±190.86 a	4 331.86±175.96 a	6.53±0.02 cd	0.005±0.000 bcd	0.813±0.002 cd	0.066±0.001 bc
JZS-T0	0.967 6	3 123.00±187.28 a	4 409.18±296.88 a	4 391.73±254.61 a	6.46±0.12 d	0.010±0.004 a	0.802±0.015 de	0.036±0.018 d
JZS-T1	0.970 9	2 999.00±184.52 a	4 260.66±277.70 a	4 209.69±243.27 a	6.58±0.03 bc	0.004±0.000 cd	0.822±0.003 bc	0.085±0.010 b
JZS-T2	0.970 0	3 011.67±127.44 a	4 273.25±239.49 a	4 240.67±228.24 a	6.52±0.06 cd	0.005±0.001 bcd	0.814±0.009 bcd	0.075±0.015 b
JZS-T3	0.969 6	3 015.67±100.95 a	4 256.61±110.02 a	4 253.03±136.65 a	6.45±0.07 d	0.006±0.001 bc	0.804±0.010 de	0.054±0.009 cd

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分组Group	R值R value	P值P value
A	0.416 7	0.001
B	0.774 4	0.001
C	0.907 2	0.001

分组Group	R值R value	P值P value
A	0.416 7	0.001
B	0.774 4	0.001
C	0.907 2	0.001