Journal of Agricultural Science and Technology ›› 2022, Vol. 24 ›› Issue (6): 82-89.DOI: 10.13304/j.nykjdb.2021.0139
• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles
Yuhong WU1,2(), Rongjun GUO2(), Guizhen MA1(), Shidong LI2
Received:
2021-02-10
Accepted:
2021-04-15
Online:
2022-06-15
Published:
2022-06-21
Contact:
Rongjun GUO,Guizhen MA
吴玉洪1,2(), 郭荣君2(), 马桂珍1(), 李世东2
通讯作者:
郭荣君,马桂珍
作者简介:
吴玉洪 E-mail:1750435224@qq.com
基金资助:
CLC Number:
Yuhong WU, Rongjun GUO, Guizhen MA, Shidong LI. Characteristics of the Growth of Rhodococcuspyridinivorans Rp3 and Ability to Degrade Skatole[J]. Journal of Agricultural Science and Technology, 2022, 24(6): 82-89.
吴玉洪, 郭荣君, 马桂珍, 李世东. 嗜吡啶红球菌Rp3生长及降解粪臭素特性研究[J]. 中国农业科技导报, 2022, 24(6): 82-89.
Fig.1 Growth of strain Rp3 cultured in broth with skatole as sole carbonNote:The different lowercase letters indicate significant difference between different treatments at P<0.05 level.
实验编号 Experiment number | A:蔗糖 Sucrose | B:牛肉膏 Beef extract | C:酵母浸粉 Yeast extract powder | 活菌数对数值 Logarithm of cultivable bacteria/ (lg CFU·mL-1) |
---|---|---|---|---|
1 | A3 | B2 | C1 | 9.55±0.02 d |
2 | A2 | B2 | C3 | 9.79±0.01 a |
3 | A1 | B2 | C2 | 9.69±0.01 b |
4 | A2 | B1 | C2 | 9.65±0.02 bc |
5 | A2 | B3 | C1 | 9.62±0.02 c |
6 | A3 | B1 | C3 | 9.81±0.02 a |
7 | A3 | B3 | C2 | 9.77±0.02 a |
8 | A1 | B3 | C3 | 9.81±0.02 a |
9 | A1 | B1 | C1 | 9.50±0.04 d |
K1 | 29.00 | 28.96 | 28.67 | — |
K2 | 29.06 | 29.03 | 29.11 | — |
K3 | 29.13 | 29.20 | 29.41 | — |
k1 | 9.67 | 9.65 | 9.56 | — |
k2 | 9.69 | 9.68 | 9.70 | — |
k3 | 9.71 | 9.73 | 9.80 | — |
极差Ri | 0.04 | 0.08 | 0.24 | — |
主次顺序 Primary and secondary order | C>B>A | |||
最优组合 Excellent combination | A3B3C3 |
Table 1 Statistical results of orthogonal test for the optimization of the medium components
实验编号 Experiment number | A:蔗糖 Sucrose | B:牛肉膏 Beef extract | C:酵母浸粉 Yeast extract powder | 活菌数对数值 Logarithm of cultivable bacteria/ (lg CFU·mL-1) |
---|---|---|---|---|
1 | A3 | B2 | C1 | 9.55±0.02 d |
2 | A2 | B2 | C3 | 9.79±0.01 a |
3 | A1 | B2 | C2 | 9.69±0.01 b |
4 | A2 | B1 | C2 | 9.65±0.02 bc |
5 | A2 | B3 | C1 | 9.62±0.02 c |
6 | A3 | B1 | C3 | 9.81±0.02 a |
7 | A3 | B3 | C2 | 9.77±0.02 a |
8 | A1 | B3 | C3 | 9.81±0.02 a |
9 | A1 | B1 | C1 | 9.50±0.04 d |
K1 | 29.00 | 28.96 | 28.67 | — |
K2 | 29.06 | 29.03 | 29.11 | — |
K3 | 29.13 | 29.20 | 29.41 | — |
k1 | 9.67 | 9.65 | 9.56 | — |
k2 | 9.69 | 9.68 | 9.70 | — |
k3 | 9.71 | 9.73 | 9.80 | — |
极差Ri | 0.04 | 0.08 | 0.24 | — |
主次顺序 Primary and secondary order | C>B>A | |||
最优组合 Excellent combination | A3B3C3 |
Fig.3 Metal ions for strain Rp3 growth and skatole degradationNote: Different lowercase letters indicate significant difference among different treatments at P<0.05 level.
Fig.4 Optical temperature for strain Rp3 growth and skatole degradationNote: Different lowercase letters indicate significant difference among different treatments at P<0.05 level.
培养时间 Incubation time/h | 处理 Treatment | Rp3菌株生长量 Growth of strain Rp3(OD600) | 粪臭素含量 Skatole content/(mg·L-1) | 降解率 Degradation rate/% |
---|---|---|---|---|
24 | Rp3-0 | 0.37±0.015 | 14.8±1.0 | 82.9±0.3 a |
CK-0 | — | 86.3±7.1 | — | |
Rp3-5 | 0.37±0.014 | 13.4±0.5 | 84.0±0.6 a | |
CK-5 | — | 83.7±0.9 | — | |
Rp3-10 | 0.36±0.014 | 13.7±0.4 | 83.2±1.3 a | |
CK-10 | — | 82.1±7.1 | — | |
48 | Rp3-0 | 0.78±0.017 | 0 | 100.0 a |
CK-0 | — | 78.0±2.3 | — | |
Rp3-5 | 0.80±0.012 | 0 | 100.0 a | |
CK-5 | — | 78.7±0.92 | — | |
Rp3-10 | 0.80±0.018 | 0 | 100.0 a | |
CK-10 | — | 78.7±0.5 | — |
Table 2 Comparison of the growth and skatole degrading ability between the initial and subculture strain Rp3
培养时间 Incubation time/h | 处理 Treatment | Rp3菌株生长量 Growth of strain Rp3(OD600) | 粪臭素含量 Skatole content/(mg·L-1) | 降解率 Degradation rate/% |
---|---|---|---|---|
24 | Rp3-0 | 0.37±0.015 | 14.8±1.0 | 82.9±0.3 a |
CK-0 | — | 86.3±7.1 | — | |
Rp3-5 | 0.37±0.014 | 13.4±0.5 | 84.0±0.6 a | |
CK-5 | — | 83.7±0.9 | — | |
Rp3-10 | 0.36±0.014 | 13.7±0.4 | 83.2±1.3 a | |
CK-10 | — | 82.1±7.1 | — | |
48 | Rp3-0 | 0.78±0.017 | 0 | 100.0 a |
CK-0 | — | 78.0±2.3 | — | |
Rp3-5 | 0.80±0.012 | 0 | 100.0 a | |
CK-5 | — | 78.7±0.92 | — | |
Rp3-10 | 0.80±0.018 | 0 | 100.0 a | |
CK-10 | — | 78.7±0.5 | — |
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