Progress on Agricultural Synthetic Biology Development and Risk Management

doi:10.13304/j.nykjdb.2022.1090

Abstract

Abstract:

As a strategic high-tech in the field of agricultural science and technology， synthetic biotechnology has the characteristics of convergent research. It provides ways and schemes for solving the worldwide agricultural problems such as photosynthesis， biological nitrogen fixation and biological stress resistance， injects new kinetic energy into promoting the sustainable development of bio-economy， and has become the strategic commanding point of global scientific and technological competition. In this paper， the research and application status， potential risk points and safety management strategies of agricultural synthetic biotechnology were summarized. The review would provide reference and support for research and development and safety management of agricultural synthetic biotechnology in China.

Key words: agricultural synthetic organism, development, potential risk, safety management

摘要：

合成生物技术作为农业科技领域的战略高新技术，具有会聚研究的特性，为解决光合作用、生物固氮、生物抗逆等世界性农业难题提供了途径和方案，为促进生物经济的可持续发展注入了新动能，已成为全球科技竞争的战略制高点。本文综述了农业合成生物技术的国内外研发与应用现状、存在的潜在风险点以及安全管理策略，以期为我国农业合成生物技术研发与安全管理提供借鉴。

关键词: 农业合成生物, 研发, 潜在风险, 安全监管

CLC Number:

Xujing WANG, Yongliang YAN, Youhua WANG, Qiaoling TANG, Yue JIAO, Zhixing WANG. Progress on Agricultural Synthetic Biology Development and Risk Management[J]. Journal of Agricultural Science and Technology, 2022, 24(12): 160-166.

王旭静, 燕永亮, 王友华, 唐巧玲, 焦悦, 王志兴. 农业合成生物研发与风险管理[J]. 中国农业科技导报, 2022, 24(12): 160-166.

References 64

1	MENG F K, ELLIS T. The second decade of synthetic biology: 2010—2020 [J/OL]. Nat. Commun., 2020,11(1): 5174 [2022-11-13]. .
2	赵国屏. 合成生物学: 开启生命科学“会聚”研究新时代[J]. 中国科学院院刊, 2018, 33(11): 1135-1149.
	ZHAO G P. Synthetic biology: unsealing the convergence era of life science research [J]. Bull. Chin. Acad. Sci., 2018, 33(11): 1135-1149.
3	赵国屏. 合成生物学:从“造物致用”到产业转化[J].生物工程学报, 2022, 38(11):4001-4011.
	ZHAO G P. Synthetic biology: from “build-for-use” to commercialization [J]. Chin. J. Biotech., 2022, 38(11): 4001-4011.
4	林敏, 姚斌. 加强合成生物技术创新, 引领现代农业跨越发展[J].生物技术进展,2022,12(3):321-324.
	LIN M, YAO B. Strengthening innovation in synthetic biotechnology and leading the leapfrog development of modern agriculture [J]. Curr. Biotech., 2022, 12(3): 321-324.
5	HOBOM B. Gene surgery: on the threshold of synthetic biology [J]. Med. Klin., 1980,75(24): 834-841.
6	刘海龙. 人工合成生命:有机生命的机械制造[J].山东科技大学学报(社会科学版), 2022, 24(4):1-11
	LIU H L. Synthesizing life: the mechanical manufacture of organic life [J]. J. Shandong. Univ. Sci. Technol. (Soc. Sci.), 2022, 24(4): 1-11.
7	MCADAMS H H, ARKIN A. Towards a circuit engineering discipline [J]. Curr. Biol., 2000, 10(8):318-320.
8	DIPINTE G, GAURI S, POOJA S, et al.. Synthetic biology in plants, a boon for coming decades [J]. Mol. Biotech., 2021, 63(12):1138-1154.
9	MARTIN V J, PITERA D J, WITHERS S T, et al.. Engineering a mevalonate pathway in escherichia coli for production of terpenoids [J]. Nat. Biotech., 2003, 21(7):796-802.
10	RO D K, PARADISE E M, OUELLET M, et al.. Production of the antimalarial drug precursor artemisinic acid in engineered yeast [J]. Nature, 2006, 440(7086):940-943.
11	GIBSON D G, GLASS J I, LARTIGUE C, et al.. Creation of a bacterial cell controlled by a chemically synthesized genome [J]. Science, 2010, 329(5987):52-56.
12	PADDON C J, WESTFALL P J, PITERA D J, et al.. High-level semi-synthetic production of the potent antimalarial artemisinin [J]. Nature, 2013, 496(7446):528-532.
13	CHEN G Q. Synthetic biology in China, UK and US [J]. Synth. Syst. Biotech., 2016, 1(4): 215.
14	周光明, 陈大明, 熊燕, 等. 英国合成生物学规划及其影响与启示[J].中国细胞生物学学报, 2019, 41(11): 2091-2100.
	ZHOU G M, CHEN D M, XIONG Y, et al.. UK synthetic biology strategic planning and its enlightenment [J]. Chin. J. Cell. Biol., 2019, 41(11): 2091-2100.
15	SHENDURE J, BALASUBRAMANIAN S, CHURCH G M, et al.. DNA sequencing at 40: past, present and future [J]. Nature, 2017, 550(7676): 345-353.
16	YU W, YAU Y, BIRCHLER J A. Plant artificial chromosome technology and its potential application in genetic engineering [J]. Plant Biotech. J., 2016, 14(5):1175-1182.
17	PALLUK S, ARLOW D H, DE ROND T, et al.. De novo DNA synthesis using polymerase-nucleotide conjugates [J]. Nat. Biotech., 2018, 36(7): 645-650.
18	VENTER J C, GLASS J I, HUTCHISON C A, et al.. Synthetic chromosomes, genomes, viruses, and cells [J]. Cell, 2022, 185(15): 2708-2724.
19	SCHMID B, ZELLER S. Faculty opinions recommendation of creation of a bacterial cell controlled by a chemically synthesized genome [J]. Science, 2010, 329(5987): 52-56.
20	JONES A M, DANIELSON J Å, MANOJKUMAR S N, et al.. Abscisic acid dynamics in roots detected with genetically encoded FRET sensors [J/OL]. Elife, 2014, 3: e01741 [2022-11-13]. .
21	MUKHERJEE P, BANERJEE S, WHEELER A, et al.. Live imaging of inorganic phosphate in plants with cellular and subcellular resolution [J]. Plant. Physiol., 2015, 167(3): 628-638.
22	WONG M H, GIRALDO J P, KWAK S, et al.. Nitroaromatic detection and infrared communication from wild-type plants using plant nanobionics [J]. Nat. Materials, 2017, 16(2): 264-272.
23	BLANCQUAERT D, VAN DAELE J, STROBBE S, et al.. Improving folate (vitamin B9) stability in biofortified rice through metabolic engineering [J]. Nat. Biotech., 2015, 33(10): 1076-1078.
24	SHEN B R, WANG L M, LIN X L, et al.. Engineering a new chloroplastic photorespiratory bypass to increase photosynthetic efficiency and productivity in rice [J]. Mol. Plant, 2019, 2 (12): 199-214.
25	MIETTINEN K, DONG L, NAVROT N, et al.. The seco-iridoid pathway from catharanthus roseus [J/OL]. Nat. Commun., 2014, 5(1):3606 [2022-11-13]. .
26	LAU W, SATTELY E S. Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone [J]. Science, 2015, 349(6253):1224-1228.
27	WANG X J, ZHANG X, YANG J T, et al.. Genetic variation assessment of stacked-trait transgenic maize via conventional breeding [J/OL]. BMC Plant Biol., 2019, 19(1):346 [2022-11-13]. .
28	WANG X J, ZHANG X, YANG J T, et al.. Effect on transcriptome and metabolome of stacked transgenic maize containing insecticidal cry and glyphosate tolerance epsps genes [J]. Plant J., 2018, 93(6):1007-1016.
29	LIU Q S, YANG X W, TZIN V, et al.. Plant breeding involving genetic engineering does not result in unacceptable unintended effects in rice relative to conventional crossbreeding [J]. Plant J., 2020, 103(6): 2236-2249.
30	LIU W X, ZHAO H, MIAO C H, et al.. Integrated proteomics and metabolomics analysis of transgenic and gene-stacked maize line seeds [J]. GM Crops Food, 2021, 12(1):361-375.
31	LI K, LUO Y B, HUANG K L, et al.. Single universal primer recombinase polymerase amplification-based lateral flow biosensor (SUP-RPA-LFB) for multiplex detection of genetically modified maize [J]. Anal. Chim. Acta, 2020, 1127: 217-224.
32	LIU F, LI K, ZHANG Y, et al.. An electrochemical DNA biosensor based on nitrogen-doped graphene nanosheets decorated with gold nanoparticles for genetically modified maize detection [J/OL]. Mikrochim. Acta, 2020, 187(10): 574 [2022-11-13]. .
33	梁晋刚,张开心,张旭冬,等.中国农业转基因生物环境安全检测标准体系现状与展望[J].中国油料作物学报, 2021, 43(1):1-14.
	LIANG J G, ZHANG K X, ZHANG X D, et al.. Current status and prospects of environmental safety standard system for agricultural genetically modified organisms in China [J]. Chin. J. Oil Crop Sci., 2021,43(1):1-14.
34	张晓磊,章秋艳,熊炜,等.转基因植物检测方法及标准化概述[J].中国农业大学学报, 2020, 25(9):1-12.
	ZHANG X L, ZHANG Q Y, XIONG W, et al.. Overview of detection methods and standardization for transgenic plants [J]. J. Chin. Agric. Univ., 2020,25(9):1-12.
35	WEI S B, LI X, LU Z F, et al.. A transcriptional regulator that boosts grain yields and shortens the growth duration of rice [J/OL]. Science., 2022, 377(6604): 8455 [2022-11-13]. .
36	SONG T Z, SHI Y Y, SHEN L K, et al.. An endoplasmic reticulum-localized cytochrome b5 regulates high-affinity K⁺ transport in response to salt stress in rice [J/OL]. Proc. Nat. Acad. Sci. USA, 2021, 118(50):e2114347118 [2022-11-13]. .
37	WU X, FENG H, WU D, et al.. Using high-throughput multiple optical phenotyping to decipher the genetic architecture of maize drought tolerance [J/OL]. Genome. Biol., 2021, 22(1):185 [2022-11-13]. .
38	JIANG S S, LI J, WANG Q Y, et al.. Maize growth promotion by inoculation with an engineered ammonium-excreting strain of nitrogen-fixing Pseudomonasstutzeri [J/OL]. Microorganisms, 2022, 10(10):1986 [2022-11-13]. .
39	WU K, WANG S S, SONG W Z, et al.. Enhanced sustainable green revolution yield via nitrogen-responsive chromatin modulation in rice [J/OL]. Science, 2020, 367(6478): 2046 [2022-11-13]. .
40	YANG J Q, XIE X Q, XIANG N, et al.. Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology [J]. Proc. Natl. Acad. Sci. USA, 2018, 115(36):8509-8517.
41	YANG J G, XIE X Q, WANG X, et al.. Reconstruction and minimal gene requirements for the alternative iron-only nitrogenase in escherichia coli [J]. Proc. Natl. Acad. Sci. USA, 2014, 111(35):3718-3725.
42	WANG C, WANG X J, ZHANG L W, et al.. Intrinsic and extrinsic programming of product chain length and release mode in fungal collaborating iterative polyketide synthases [J]. J. Am. Chem. Soc., 2020, 142(40):17093-17104.
43	WANG X J, WANG C, DUAN L X, et al.. Rational reprogramming of O-methylation regioselectivity for combinatorial biosynthetic tailoring of benzenediol lactone scaffolds [J]. J. Am. Chem. Soc., 2019, 141(10):4355-4364.
44	CAI T, SUN H, QIAO J, et al.. Cell-free chemoenzymatic starch synthesis from carbon dioxide [J]. Science, 2021, 373(6562):1523-1527.
45	ZHU Q, ZENG D, YU S, et al.. From golden rice to aSTARice: bioengineering astaxanthin biosynthesis in rice endosperm [J]. Mol. Plant., 2018, 11(12):1440-1448.
46	ZHANG T, YAN Y Y, HE S, et al.. Involvement of the ammonium transporter AmtB in nitrogenase regulation and ammonium excretion in pseudomonas stutzeri A1501 [J]. Res. Microbiol., 2012, 163(5):332-339.
47	燕永亮,田长富,杨建国,等.人工高效生物固氮体系创建及其农业应用[J].生命科学,2021,33(12):1532-1543.
	YAN Y L, TIAN C F, YANG J G, et al.. Establishment of artificial efficiency biological nitrogen fixation system and its agricultural application [J]. China Bull. Life Sci., 2021,33(12):1532-1543.
48	燕永亮,王忆平,林敏.生物固氮体系人工设计的研究进展[J].生物产业技术,2019 (1):34-40.
	YAN Y L, WANG Y P, LIN M. Advancement in artificial design of biological nitrogen fixation system [J]. Biotech. Business., 2019 (1):34-40.
49	马德英,于长磊,艾鹏飞.转C₄基因水稻的研究现状及展望[J].分子植物育种,2017,15(10):3997-4002.
	MA D Y, YU C L, AI P F. Progress and perspectives of transgenic rice overexpressing C₄ photosynthetic genes [J]. Mol. Plant. Breed., 2017, 15(10): 3997-4002.
50	雷瑞鹏,邱仁宗.合成生物学的伦理和治理问题[J].医学与哲学,2019,40(19):38-43.
	LEI R P, QIU R Z. Ethical and governance issues in synthetic biology [J]. Med. Philosophy., 2019,40(19):38-43.
51	刘旭霞,秦宇. 欧美合成生物学应用的风险治理经验及启示[J].华中农业大学学报(社会科学版),2022 (2):177-184.
	LIU X X, QIN Y. Risk governance experience and enlightenment of synthetic biology application in Europe and the US [J]. J. Huazhong. Agric. Univ (Soc. Sci.), 2022 (2):177-184.
52	焦悦,付伟,翟勇. RNAi技术在作物中的应用及安全评价研究[J].作物杂志, 2018 (1):9-15.
	JIAO Y, FU W, ZHAI Y. Application of RNAi in crop breeding and its safety assessment [J]. Crops, 2018 (1):9-15.
53	EPSTEIN M M, VERMEIRE T. Scientific opinion on risk assessment of synthetic biology [J]. Trends. Biotechnol., 2016, 34(8):601-603.
54	马延和,江会锋,娄春波,等.合成生物与生物安全[J].中国科学院院刊,2016,31(4):432-438.
	MA Y H, JIANG H F, LOU C B, et al.. Synthetic life and biosecurity [J]. Bull. China Acad. Sci., 2016,31(4):432-438.
55	MALARKEY T. Human health concerns with GM crops [J]. Mutat. Res., 2003, 544(2):217-221.
56	GOMEZ-TATAY L, HERNANDEZ-ANDREU J M. Biosafety and biosecurity in synthetic biology: a review [J]. Critical Rev. Environ. Sci. Technol., 2019, 49(17):1587-1621.
57	马丽丽,欧亚昆,任怡佳,等.合成生物学的生物安全风险及其管理对策研究[J].科学与社会, 2022, 12(3):15-32.
	MA L L, OU Y K, REN Y J, et al.. Research on biosafety and management countermeasures of synthetic biology [J]. Sci. Soc., 2022,12(3):15-32.
58	祁潇哲,贺晓云,黄昆仑.转基因生物食用安全评价技术体系及其发展趋势[J].中国农业大学学报,2019,24(7):71-78.
	QI X Z, HE X Y, HUANG K L. Food safety assessment technology system of genetically modified organisms and its development trend [J]. J. Chin. Agric. Univ., 2019,24(7):71-78.
59	OYE K A, ESVELT K, APPLETON E, et al.. Biotechnology begulating gene drives [J]. Science, 2014, 345(6197):626-628.
60	张维,杨敬宇,化得良.合成生物学治理的国外经验综述及启示[J].医学与哲学,2020,41(3):54-58.
	ZHANG W, YANG J Y, HUA D L. Review and implication of synthetic biology governance in foreign countries [J]. Med. Philosophy., 2020,41(3):54-58.
61	关正君,裴蕾,魏伟,等. 合成生物学概念解析、风险评价与管理[J].农业生物技术学报, 2016, 24(7): 937-945.
	GUAN Z J, PEI L, WEI W, et al.. Concept analysis, risk assessment and regulation of synthetic biology [J]. J. Agric. Biotech., 2016, 24(7): 937-945.
62	王盼娣,熊小娟,付萍,等.《生物安全法》实施背景下对合成生物学的监管[J].华中农业大学学报, 2021, 40(6):231-245.
	WANG P D, XIONG X J, FU P, et al.. Regulation of synthetic biology under background of implementing biosafety low [J]. J. Huazhong. Agric. Univ., 2021, 40(6):231-245.
63	EFSA Scientific Committee, MORE S, BAMPIDIS V, et al.. Evaluation of existing guidelines for their adequacy for the food and feed risk assessment of microorganisms obtained through synthetic biology [J/OL]. EFSA J., 2022, 20(8): e07479 [2022-11-13]. .
64	EFSA Panel on Genetically Modified Organisms GMO), MULLINS E, BRESSON J L, et al.. Evaluation of existing guidelines for their adequacy for the molecular characterisation and environmental risk assessment of genetically modified plants obtained through synthetic biology [J/OL]. EFSA J., 2021, 19(2): e06301 [2022-11-13]. .

[1]	Zhaoli AN, Dongqun YANG. Innovative Mechanism and Model of Cambodia-China Tropical Eco-agriculture Cooperation Demonstration Zone [J]. Journal of Agricultural Science and Technology, 2022, 24(8): 1-8.
[2]	Shuxin QI, Xiaolei WEN, Tingfeng JI, Zengzhi SI, Chunming ZHAO, Yake QIAO, Yanmin WANG, Aijun CAI, Haihua ZHANG, Zhixin JI. Effect of Fox Raccoon Manure on the Growth and Development of Black Soldier Fly [J]. Journal of Agricultural Science and Technology, 2022, 24(8): 201-206.
[3]	Ruixue ZHAO, Tan SUN, Xiaorong YANG, Jian WANG. Research on the Development Path of Agricultural Scientific Informatization Under Data-intensive Environments [J]. Journal of Agricultural Science and Technology, 2022, 24(7): 1-7.
[4]	Shuai WANG, Wei SONG, Ronghuan WANG, Jiuran ZHAO. Progress of Maize Biology Research in China [J]. Journal of Agricultural Science and Technology, 2022, 24(7): 23-31.
[5]	Nan WU, Jun YANG, Yan ZHANG, Zhengwen SUN, Dongmei ZHANG, Lihua LI, Jinhua WU, Zhiying MA, Xingfen WANG. Overexpression of a Cotton Glucuronokinase Gene GbGlcAK Promotes Cell Elongation in Arabidopsis thaliana [J]. Journal of Agricultural Science and Technology, 2022, 24(6): 36-46.
[6]	Jianhua ZHENG, Ruixue ZHAO, Hua ZHAO, Sijing LI, Guogang WANG. International Experience and Enlightenment on Development of Smart Agricultural Information Services [J]. Journal of Agricultural Science and Technology, 2022, 24(6): 9-18.
[7]	Yuqin XIAO, Xiao LEI, Mingjin ZHANG, Yuangai ZHANG, Shan TANG, Hongfei JI, Chuan WANG, Cuiling MA, Yanqiu JING. Effects of Three Bacillus Agents on Seedling Growth of Flue-cured Tobacco [J]. Journal of Agricultural Science and Technology, 2022, 24(5): 85-92.
[8]	Fangling WANG, Mingyue ZHANG, Yaru ZHOU, Qinglin GUAN, Xinyan LI, Qiu ZHONG, Mingqin ZHAO. Effect of TS-PAA Water Retaining Agent on Growth and Photosynthetic Characteristics of Cigar under Drought Stress [J]. Journal of Agricultural Science and Technology, 2022, 24(4): 162-172.
[9]	Xuezhen XIONG, Chun YANG, Xiaoping MA. Situation of China's Animal Husbandry Development and High-quality Development Strategy Selection [J]. Journal of Agricultural Science and Technology, 2022, 24(3): 1-10.
[10]	Yiping DUAN, Mingliang WU, Jiangnan LYU, Wei XIANG, Bo YAN, Lan MA, Jiajie LIU. Research Status and Development Suggestions of No-tillage Seeding Anti-blocking Technology [J]. Journal of Agricultural Science and Technology, 2022, 24(2): 124-135.
[11]	Zhiyong WU, Hong GU, Dawei CHENG, Lan LI, Shasha HE, Ming LI, Jinyong CHEN. Advances in Regulatory Mechanism of Brassinolide on Plant Root Development [J]. Journal of Agricultural Science and Technology, 2022, 24(2): 68-76.
[12]	Hua XIANG, Yan YAN, Xiudong WANG. Evaluation of Industrial Chain Integration Driven by Technology Integration of Characteristic Tropical Crops [J]. Journal of Agricultural Science and Technology, 2022, 24(11): 1-12.
[13]	Lei SHI, Yanyan SUN, Yunlei LI, Hao BAI, Jilan CHEN. Research Progress on Crossed Beak in Chicken [J]. Journal of Agricultural Science and Technology, 2022, 24(11): 35-42.
[14]	Pei XIE, Zihan SONG, Mingmin XIONG. Achievements and Prospects on China’s Seed Industry Development at Different Stages [J]. Journal of Agricultural Science and Technology, 2022, 24(10): 1-5.
[15]	LIU Zhengwen, WANG Xingfen, MENG Chengsheng, ZHANG Yan, SUN Zhengwen, WU Liqiang, MA Zhiying, ZHANG Guiyin. Genome-Wide Identification and Analysis of GH9 Gene Family in Gossypium barbadense L. [J]. Journal of Agricultural Science and Technology, 2021, 23(9): 30-45.