Table of Content

15 February 2011, Volume 13 Issue 1

Previous Issue    Next Issue

Studies on Genetic Transformation Bt cry1Ⅰa Gene into Cauliflower by Agrobacterium-mediated Transformation

ZHANG Yong-xia1,2, LANG Zhi-hong1, ZHU Li1, YANG Li-mei3, ZHANG Jie4,

2011, 13(1):  15-19.  DOI: 10.3969/j.issn.1008-0864.2011.01.03

Asbtract ( )   PDF (609KB) ( )  

Related Articles | Metrics

The double T-DNA border expression vector pCS1Ⅰa-LR harboring Bacillus thuringiensis cry1Ⅰa gene was introduced into the cotyledon petioles and hypocotyls of cauliflower（Brassica oleracea var. botrytis) mediated by Agrobacterium. Thirty regenerated plants were obtained, 18 of them were positive by PCR detection, which demonstrated that cry1Ⅰa gene was integrated into the cauliflower genome. The PCR positive rate is 60%. Moreover, RT-PCR and Western blot analysis showed that cry1Ⅰa gene were transcripted and translated, respectively in transgenic cauliflower. Bioassay results showed that the transgenic cauliflowers harboring cry1Ⅰa gene had the high toxicity to diamondback moth and the mortality of insect larvae was accounted for 100％. The transgenic cauliflower can be used for further insect resistant cauliflower breeding.

Experiment on Greenhouse Solar Energy Associated with Air-source Heat Pump Heating System

CHEN Bing, LUO Xiao-lin, BI Fang-lin, LI Jie

2011, 13(1):  55-59.  DOI: 10.3969/j.issn.1008-0864.2011.01.09

Asbtract ( )   PDF (433KB) ( )  

Related Articles | Metrics

In order to explore the application methods and development potential of solar energy and air source heat pump (ASHP) technology in facility agriculture, and to seek a solution to resolve problems existing in greenhouse production, such as high heating cost and environment pollution etc., an experiment was carried out on a solarair source heat pump heating system used for greenhouse. In this paper, the overall design and operation method for the system were introduced, and an experiment on the system's operation performance and heating efficiency in greenhouse was tested in Kunming. The results showed that in January, the coldest month in Kunming, the average temperature of a heat storage tank could reach up to 41.1℃，and the average of COP were over 3 when the ASHP was running. Whether it is sunny or cloudy, the temperature inside of greenhouse can meet the needs for crop development. Thus, a theoretical basis is provided for the research on solar energy associated with airsource heat pump heating system.

Studies on Groundwater Nitrate Nitrogen Distribution and its Affecting Factors in Chao Lake Watershed

GU Ying, SUN Dong-bao, WANG Qing-suo

2011, 13(1):  68-74.  DOI: 10.3969/j.issn.1008-0864.2011.01.11

Asbtract ( )   PDF (641KB) ( )  

Related Articles | Metrics

To investigate the spatial distribution of groundwater nitrate nitrogen (NO-3N) in the Chao Lake watershed, 253 water samples were collected from wells in the watershed from November to December, 2009 and their NO-3N concentrations were analyzed. The results showed that the samples NO-3N concentrations were averaged 7.13 mg/L, and 15.81% of them exceeded 10 mg/L, the WHO limit for NO-3N of drinking water, and 7.11% of them exceeded 20 mg/L, the criterion for serious NO-3N pollution. The NO-3N concentrations were different in different land types, with the pattern of villages>vegetable fields>fruit gardens>dry lands>towns>ricerape (or wheat) fields>singleseason rice fields>livestock farms. The NO-3N concentration of groundwater in Green RiceProducing Area was lower than that in NonGreen RiceProducing Area in Chao Lake watershed. The NO-3N concentration of groundwater in farmlands was positively related to the application rate of nitrogen chemical fertilizer, the population density and the proportion of cultivated area. The  NO-3N concentrations were declined with the lowering of the groundwater table, but there was no significant correlation between them. When the application of chemical fertilizer nitrogen exceeds 100 kg/hm2, or the groundwater table is less than 9 m, the NO-3N concentration of groundwater has the potential risk of exceeding the WHO limit.

Prospect for Present Status and Application of Polysaccharides from Nostoc

LI Hai-feng1, LI Zhang-wei1,2, LIU Yong-mei1, HUANG Ze-bo1

2011, 13(1):  105-110.  DOI: 10.3969/j.issn.1008-0864.2011.01.17

Asbtract ( )   PDF (606KB) ( )  

Related Articles | Metrics

Nostoc polysaccharides are the main supporting matrix of the edible Nostoc colonies and key bioactive components. They have a number of bioactivities, such as immunomodulation, anticancer and antiviral activities, and play important roles in protecting Nostoc cells from physical and biological stresses. This paper reviewed the progress of isolation, structure and bioactivity of Nostoc polysaccharides, and prospected their potential applications in food, pharmaceutical and cosmetic industries.

Detection of Shewanella smarflavi AP629 by Double-antibody Sandwich ELISA Method

WU Qiu-xian, LI Qiang, LI Hua, WANG Yi-nan

2011, 13(1):  117-121.  DOI: 10.3969/j.issn.1008-0864.2011.01.19

Asbtract ( )   PDF (472KB) ( )  

Related Articles | Metrics

At present, skin ulceration syndrome is the most serious disease in Apostichopus japonicus aquaculture. It caused mass mortality and great economic losses for aquaculture in China. A doubleantibody sandwich enzymelinked immunosorbent assay (DAS-ELISA) was developed for rapidly detecting S.smarflavi (AP629), a pathogen of Apostichopus japonicus, using polyclonal antibody (pAb) from rabbit and monoclonal antibody 3D9 (mAb 3D9) from mouse against AP629. The optimal dilution of pAb and mAb 3D9 were 1∶400 and 1∶80, respectively. This method has strong specificity, no cross reaction with other bacteria, including Vibrio sp, Aeromonas salmonicida, Edwardsiella ictaluri, and Escherichia coli etc., and high sensitivity in detection. The lowest concentration of strain AP629 that can be detected was 104 cells/mL and 106 cells/mL, respectively using PBS and homogenate of body wall from A. japonicus as medium. 10 artificial infected A.japonicus samples were detected and 100% of them were positive. So it has better stability and repetition. This method is very helpful for rapid and accurate diagnosis of A. japonicus infected by S.smarflavi AP629.

Drug Residue Detecting Technology Based on Combing |Molecular Imprinted Polymer Film with Reflectometric Interference Spectroscopy

LIU Qiu-ming1, WANG Yong-xiang1,2, DING Yan1, RONG Fei1,2, FU De-gang1,2

2011, 13(1):  122-128.  DOI: 10.3969/j.issn.1008-0864.2011.01.20

Asbtract ( )   PDF (705KB) ( )  

Related Articles | Metrics

To measure optical thickness of a molecularly imprinted polymer film whose surface was not smooth with porous structure, a ladder model for RIfS (reflectometric interference spectroscopy) was established, and a computer program for full-spectrum fitting of RIfS spectra was written based on damped least square method. Then the optical thickness of a monolayer or bilayer film could be obtained by fitting the experimental spectrum data. Sensitive films were fabricated by spin coating the mixture of CAP (chloramphenicol) imprinted nanospheres and polystyrene (PS) onto glasses and silicon wafers, respectively. Linear relationships between variance of film optical thickness and concentrations of CAP in solution were founded. The detection limits were 0.229 1 mmol/L and 0.185 2 mmol/L, respectively. Then a real sample (1 g milk fortified with 0.2 mL 0.5 mol/M CAP) was analyzed by this method. After diluting the extracted liquid and combing the standard curve, the CAP recovery was 91%, which agreed with that determined by HPLC. This result indicated that the combination of molecularly imprinted polymer films and RIfS was feasible in analyzing drugs residue.

Studies on Simulation Technique of Tree Single Root Imaging by Ground Penetrating Radar

BAI Xue1,2, JI Yi-cai1, FANG Guang-you1

2011, 13(1):  129-136.  DOI: 10.3969/j.issn.1008-0864.2011.01.21

Asbtract ( )   PDF (873KB) ( )  

Related Articles | Metrics

Diffraction tomography (DT) algorithm is derived for the detection and imaging of tree roots using ultrawide band (UWB) ground penetrate radar (GPR) technique. A mathematical analysis model of the root in soil was built based on the finite difference time domain (FDTD) to obtain the GPR data. And the DT algorithm was used to reconstruct crosssectional image of root. Limiting factors in the detection and reconstruction of roots, such as root diameter, permittivity and intervals were also discussed in the paper. The simulation results showed that, in nearly lossless homogeneous soil, UWB GPR combining with DT algorithm leaded to a rapid and accurate reconstruction of root′s position, shape and permittivity.