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    15 April 2015, Volume 17 Issue 2
    Research Progress in Plant Hybrid Lethality
    XUE Yu-qian, ZHUANG Mu*, FANG Zhi-yuan, LIU Yu-mei, YANG Li-mei, ZHANG Yang-yong
    2015, 17(2):  1-7.  DOI: 10.13304/j.nykjdb.2014.558
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    Plant hybrid lethality is a sub-type of reproductive isolation, which belongs to the post zygotic reproductive isolation, and plays an important role in speciation and maintaining integrity of species, but also is a challenge encountered by the breeders in the process of developing new varieties. Studying the hybrid lethality existing in multiple species can help to understand the occurance mechanism of reproductive isolation, and apply lethal gene in crop breeding program can provide theoretical guidance for crop improvement. This paper expounded the hybrid lethality cases, lethality influence factors, genetic pattern research, gene mapping, etc., and prospected the application of modern biological information technique to hybrid lethality phenomenon, so as to further reveal the occurance mechanism of hybrid lethality and the structure, and function of lethal gene.

    Progress and Prospect in Agricultural Internet of Things
    CHEN Xiao-dong1, YUAN Xiang-yang1, GUO Ping-yi1*, NING Na1, GUO Mei-jun1, LAN Ya
    2015, 17(2):  8-16.  DOI: 10.13304/j.nykjdb.2014.359
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    The internet of things (IOT) technology marks another great leap of the global information industry after computer and internet. IOT presents unprecedented opportunities for rapid development of agricultural modernization. This paper briefly introduced the concept and architecture of IOT in agriculture; analyzed the key techniques of IOT in agriculture from three levels as information awareness, network transmission and processing application; summarized the present research at home and abroad, and applying status and existing problems of IOT in agriculture; put forward the development strategy and key measures for its development in China; and pointed out that IOT in agriculture would become more thorough and comprehensive perception, with more safe and reliable connectivity, more optimization of system integration, and its development trends would be more in-depth intelligence services. Thus, IOT in agriculture would surely play more important role in promoting the fusion of informationization and agricultural modernization, and in accelerating the development of agricultural modernization.

    Progress on Jasmonic Acid Signaling in Plant Stress Resistant
    SONG Yun1, LI Lin-xuan2, ZHUO Feng-ping2, ZHANG Xue-yan1, REN Mao-zhi2, LI Fu-gu
    2015, 17(2):  17-24.  DOI: 10.13304/j.nykjdb.2014.610
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    Jasmonic acid (JA), acting as an important new endogenous hormone, fulfills the diversity of roles in plant defense against pathogens and insects. In recent years, more and more studies have shown that JA also plays an important role in plant development and osmotic stress. To advance our understanding of these novel functions of JA, this paper described how the key regulators of JA signaling pathway regulate photosynthesis and the development of root and stomata in plants in this review. The progress was expected to provide some new insights for us to understand the crosstalk between JA signaling and plant development and osmotic stress.

    Mechanism of Brassinosteroid Involved in Regulating Plant Development, Stress Resistance and its Application in Breeding
    LI Yuan-yuan1,2, CAO Qing-he1,2*
    2015, 17(2):  25-32.  DOI: 10.13304/j.nykjdb.2014.560
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    Brassinosteroids (BRs) is a major plant growth-promoting hormone regulating developmental and physiological processes. The recent studies on BRs indicated that it could increase crop yield and enhance crop resistance to stress. Protein kinase BRI1 and BAK1 initiated outgoing intracellular BRs signal transduction, and the downstream transcription factor BZR1 and BZR2/BES1 were responsible for gene regulation. There were key phosphorylation sites of receptor kinase and TFs responsible for BR signaling, which could be simply manipulated by mutagenesis. Dissect LRR-kinase signal transduction based on protein structure and gene function, and through protein modification based on site-direction mutation can benefit for elucidating BR signaling pathway and network, and eventually improve the plant adaptation for stress environment. This paper summarized studies on BRs signaling pathways and its relationship with plant development and environmental stress, hoping to provide good references for plant molecular breeding.

    Expressive Characteristics of Drought-related Genes and Their Genetic Engineering Prospects in Crops
    MA Yi-wen1,2, LI Liang2, WANG Nan2, WANG Zhen-ping3, JIANG Li-yan3, HAO Zhuan-fa
    2015, 17(2):  33-40.  DOI: 10.13304/j.nykjdb.2014.707
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    Drought restricts crop growth and yield formation, and causes a series of physiological and biochemical reactions inside of crop. Drought tolerance of crops is a complex quantitative trait controlled by multiple genes. In order to cope with water deficiency, crops form multiple signaling pathways for surviving in morphology, physiology and molecular level. Under drought stress, crops activate transcription factors to regulate expression of the corresponding downstream genes, and being adapt to drought via the post-transcriptional regulation and osmotic adjustment, and maintain normal physiological and metabolic activities to reduce damage by drought stress. Stress can also induce the generation of small RNA, which regulates its target genes by inducing degradation of target gene mRNA and prevents translation, reaches the function of stress tolerance. Genetic engineering of crop drought-related genes, which can grow well under abiotic stresses, is very important. This paper summarized the function and application of genes involved in different drought response mechanisms, which will provide references for drought tolerance breeding.

    Map-based Cloning and Function Analysis of Rice Yellow Leaf Mutant yl1
    WANG Dan-xia, QUAN Rui-dang*, HUANG Rong-feng
    2015, 17(2):  41-48.  DOI: 10.13304/j.nykjdb.2014.683
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    Leaf, an crucial organ for photosynthesis, provides the basis of crop yield formation. In addition to a trait marker in breedings, leaf color mutants in crops are one of the prerequisites for investigation in chloroplast development and cultivation of new germplasms with higher photosynthetic efficiency. A yellow leaf mutant (yl1) were selected from a pool of Japonica rice Kitaake mutagenized with ethyl methanesulfonate(EMS). The yl1 mutant showed lower plant height, less grain number and less 1 000-grain weight compared with wild type. Genetic analysis showed that yellow leaf phenotype in yl1 mutant was caused by a single recessive gene. Map-based cloning demonstrated that the coding region of OsCAO1 (LOC_Os10g41780), encoding chlorophyllide a oxygenase, had a G to A point mutation site (Glycine 396 to Arginine in amino acid sequence) in yl1. Sequence analysis and GFP experiment indicated the mutation site G396R of CAO1 in yl1 is in catalytic domain, and made no change in CAO1 location in chloroplast. Therefore, G396 might be the key point for OsCAO1 enzyme activity as G396 was highly conserved in different species.

    Protein Structure and Gene Expression Pattern Analysis of Gossypium raimondii GPI-anchor Lipid Transfer LTPG Family
    DENG Ting1, WANG Jin2, WANG Jun1, ZHOU Yu-mei1, ZUO Kai-jing1*
    2015, 17(2):  49-57.  DOI: 10.13304/j.nykjdb.2014.657
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    A lot of research indicates that lipids are involved in cotton fiber initiation and elongation. LTPG (GPI-anchored lipid transfer protein) is involved in lipid transportation during cell enlargement, so the protein structure and gene expression pattern of LTPG gene family in Gossypium raimondii were analyzed. The results showed that LTPGs were classified into 8 subgroups, and the position /distribution of each subgroup protein had its own typical molecular weight and conserved cysteine motif. Promoter elements analysis indicated that LTPGs contained the conservative binding sites for MYB transcription factors and hormone response elements. RNA-seq data revealed that three expression patterns of LTPGs existed during fiber development, suggesting their roles in fiber initiation and elongation.

    Regulation Function of Honeybee Gut Microbiota and its Influence Factors
    GUO Jun1,2, WU Jie1*, LIU Shan1, LI Ji-lian1*
    2015, 17(2):  58-63.  DOI: 10.13304/j.nykjdb.2014.557
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    Recently, substantial reduction of honeybee numbers has led to big economic losses in apiculture industry worldwidely and brought about severe consequences in many areas of agriculture. The latest research findings indicated that gut microbiota was closely related to the health of the host. This paper reviewed the species and characteristic of honeybee gut microbiota, expounded the regulation function of gut microbiota in honeybee health and factors influencing gut microbiota. The paper also prospected the future research orientation.

    Cloning of Laccase Lac13H9 Gene from Soil Metagenome and Analysis of its Enzymatic Properties
    HU Bin-bin1,2§, WU Ping2§, LIU Xiao-qing2, DING Wei1*, WU Ning-feng2*
    2015, 17(2):  64-71.  DOI: 10.13304/j.nykjdb.2014.743
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    Laccase widely exists in soil and has potential industrial value in degradation of organic pesticide residues and lignin. In this study, a laccase gene, lac13H9, with total length of 1 389 bp was obtained from the soil macro genome. It encoded 462 amino acids polypeptide with a calculated molecular mass of 50 kDa and contained a signal peptide composed with 17 amino acid residues. The deduced amino acid sequence was a new protein through aligning with available protein sequences held in the NCBI. The recombinant lac13H9 heterologously expressed in Escherichia coli Transseta (DE3) and purified by the affinity chromatography on a Ni-NTA column. The maximal activity of laccase was observed at 40℃, the optimum pH was 6.0, and it had good thermal stability. About 60% enzyme activity was remained under 50℃ for 90 min. At the same time, low concentration of Fe2+ could promote laccase enzyme activity, while high concentration could inhibit enzyme activity. Fine enzymatic property of Lac13H9 has laid foundation for its application.

    Heterologous Expression of 6-phosphofructo Kinase Gene pfkA from Escherichia coli in Pseudomonas stutzeri and Phenotype Analysis of Recombinant Strain
    HU Tao1,2, MA Yao2, ZHAN Yu-hua2, LU Wei2, HUANG He1, LIN Min2, YAN Yong-liang2*
    2015, 17(2):  72-80.  DOI: 10.13304/j.nykjdb.2015.088
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    Sugar metabolism is the process operated by nearly all organisms for the supplement of nutrition and energy. As one of the most widely used monosaccharides in nature, glucose is an ideal carbon source for microorganisms. Pseudomonas stutzeri A1501 has the canonica microbial metabolic pathways. However, it has a very low rate for glucose utilization. Genome analysis of P. stutzeri A1501 suggested that A1501 could not use glucose by the Embden-Meyerhof-Parnas (EMP) pathway, because it lacked the key glycolytic enzyme 6-phosphofructokinase encoded by pfkA. An Escherichia coli pfkA gene was cloned and transferred to P. stutzeri A1501 to accomplish the reconstruction in vivo of the complete set of enzymes for enabling a functional EMP pathway in A1501. The expression of pfkA in P. stutzeri A1501 failed to glucose utilization by the EMP pathway, suggesting that there were other systems that might affect the glucose utilization except for the pfkA gene. It was also observed that the recombinant A1501-pfkA strain became more sensitive to hydrogen peroxide, compared to the wild type. We further hypothesized that the major reason for pfkA confers decreased oxidative tolerance to P. stutzeri A1501 was due to the influence of its NADPH formation.

    Observation on the Infection Cycle of Caenorhabditis elegans by Nematophagous Fungus Drechmeria coniospora Using Scanning Electron Microscope
    GUO Qian-nan, ZHOU Zheng-fu, XU Yu-quan, ZHANG Wei*
    2015, 17(2):  81-86.  DOI: 10.13304/j.nykjdb.2014.714
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    Nematophagous fungus plays a key role in the area of nematode biological control, and has received increasing attention in recent years. Drechmeria coniospora is a typical obligate endoparasitic fungus, which specifically infects and parasites in its host nematodes. This study observed the entire process of D. coniospora infecting Caenorhabditis elegans by using high resolution scanning electron microscope (SEM). Thus, a direct and objective evidence was obtained for further studies on infection by D. coniospora and relevent genes involved in infection. The SEM images showed that D. coniospora recognized nematode by conidia, then adsorbed nematodes mouth cavity or vulva, and got into nematode. Conidia initially germinated and formed trophic hyphae inside the nematode. When trophic hyphae growth to certain extent, mycelia penetrated nematode cuticle, which led to the death of the host. Aerial mycelia produced new conidia. After these conidia were matured, another infection cycle began. The specific infection pattern and efficient infection ability of D. coniospora made it as a potential and superior strain for biological control against nematode. D. coniospora has a broad application prospect.

    Design and Implementation of Knowledge Map on Cloud Platform of Agricultural Technology Extension
    WANG Liang, LI Xiu-feng*, WANG Wen-shen
    2015, 17(2):  87-93.  DOI: 10.13304/j.nykjdb.2014.639
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    The Cloud Platform of Agricultural Technology Extension is a comprehensive knowledge platform serving for the agricultural technicians all over China. It provides various knowledge services, such as knowledge learning, exchange, sharing and creating opportunities of interacting with agricultural experts. Along with the implementation of various projects, the data quantity increased rapidly. The platform urgently needs an effective knowledge management tool to help the technicians in finding the most needed knowledge in a short time. Knowledge map (K-Map), as a knowledge management tool, has been applied in many fields. These maps can provide users an intuitive and graphical knowledge guide, reveal the relationship between knowledges, and promote knowledge exchange and sharing. This paper introduces the Cloud Platform of Agricultural Technology Extension, and the development and characteristics of K-Map. On these basis, this paper put up an idea about applying K-Map to the fields of agricultural science, and designed a scheme of constructing K-Map in the Cloud, including design of K-Map total structure, extract of position and content attributes among static knowledge nodes, social tagging of dynamic knowledge nodes, design of knowledge network correlation algorithm. A strategy of showing K-Map was illustrated in detail. Besides,K-Map on Cloud Platform of Agricultural Technology Extension had been initially realized according to the scheme.

    Design and Realization of a High-throughput Maize Kernel Trait Extraction System
    WANG Ke, LIANG Xiu-ying, ZONG Li, YANG Wan-neng*
    2015, 17(2):  94-99.  DOI: 10.13304/j.nykjdb.2015.008
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    To achieve high-throughput measurement of maize kernel phenotypic traits, a maize kernel traits system was designed based on line-scan technology combined with automatic control technology. The maize kernels were fed successively with vibrating feeder, and the undistorted images of corn kernels were acquired by matching the conveyor speed and line-scan speed via servo drive technology. Through image processing technique, the maize kernel phynotypic traits parameter measurement were realized. The experimental results showed that the measurement accuracy (mean absolute percentage error, MAPE) of total kernel number, long axis, short axis, and length-width ratio were 0.50%, 1.22%, 3.34%, and 4.22%, respectively. The measurement efficiency of the system was 12 s per ear. This study is of great significance for promoting the high-throughput identification of maize kernel traits.

    Review on Studying Image Segment Algorithms of Apple Trees
    HU Lin, ZHOU Guo-min, QIU Yun, FAN Jing-chao, GUO Xiu-ming
    2015, 17(2):  100-108.  DOI: 10.13304/j.nykjdb.2014.498
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    Based on the studies about the application of image processing and computer vision on apple trees, the paper summarized the research progress made in the image segmentation algorithms for apple tree images. According to the technical system of image segmentation, this paper analyzed the main progress made in studying the apple tree image segmentation; systematically summarized the image segmentation algorithms and characteristics of apple tree application. The paper also drew an evaluation map for apple tree image segmentation algorithms, and prospected the development trend of studying apple tree image segmentation algorithms. All those provided references for further studies on the apple informatization theory and technology.

    Research Progress on Effect of Nitrogen Form on Plant Growth
    XING Yao, MA Xing-hua*
    2015, 17(2):  109-117.  DOI: 10.13304/j.nykjdb.2014.574
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    Ammonium and nitrate, as the main inorganic nitrogen forms absorbed from the soil, had different effects on morphological and physiological processes in higher plants. This paper reviewed the mechanism of different nitrogen forms absorption and utilization in plants, how nitrogen form regulating nutrient uptake, root system development, photosynthesis physiology, yield and quality formation, and nitrogen transporter protein genes expression etc.. The paper also elaborated the mechanism of nitrogen forms regulating plant growth, and put forward problems needed to be clarified in studying nitrogen form.

    Characteristics of Different Humic Acid and its Effects on Soil and Crops
    LUO Yu1, LI Xiao-liang2, ZHANG Shan-shan1,3, ZHU Ming1, LV Yi-zhong3, DAI Zhong-
    2015, 17(2):  118-125.  DOI: 10.13304/j.nykjdb.2014.474
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    Based on the different types of humic acids, including mineral-humic acid, bio-humic acid, the study compared the characteristics and the effects of difference humic acid fertilizers on plant growth and soil fertility with the chemical fertilizer and organic fertilizer. The results showed that, compared with the chemical fertilizer,  the soil organic matter increased 11.14% and 12.03%, total N concentration in soil increased 20.62% and 22.26%, rapid available K concentration in soil increased 6.02% and 9.28%, rapid available P concentration in soil increased 2805% and 29.49% of the mineral-humic acid and bio-humic acid fertilizer treatment. Soil physical and chemical properties was also improved. The bio-humic acid fertilizers could achieve a better effect than humic acid and other organic fertilizer with inorganic fertilizer. The humic acid fertilizer could increase the yield of rape, and N,P,K concentration in plant. After 60d pot experiment, the dry matter increased 9.43% and 16.38% of the mineral-humic acid and bio-humic acid fertilizer treatment. Compared with the organic fertilizer, humic acid had better effects on crop nutrient content. Compared with mineral-humic acid, bio-humic acids even had broad prospects in soil remediation and water purification in China, saline alkali soil improvement, for example.

    Research Progress on Preparing Biochar and its Effect on Soil Physio-Chemical Properties
    WANG Rui-feng1,2, ZHAO Li-xin1, SHEN Yu-jun1, MENG Hai-bo1*, YANG Hong-zhi2
    2015, 17(2):  126-133.  DOI: 10.13304/j.nykjdb.2014.624
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    Recently, biochar as a new soil conditioner and adsorbent has become a hot spot concerned in agriculture, environment, energy and other fields. Biochar is produced by organic matter pyrolysis in absence of oxygen. Different raw materials and production conditions will produce biochar properties. Biochar can improve soil fertility, bulk density, total porosity and water retention, and also can adjust soil pH, enhance soil cation exchange capacity (CEC), improve soil nutrients. This paper summarized biochar producing method, its physical and chemical properties and its effect on soil physical and chemical properties, thus provided theoretical support to optimize biochar, upgrade the added value of it products, and promote the soil improvement.

    Structural Analysis of Seawater Bacterial Community from Culture Pond of Apostichopus japonicus at Different Periods
    WANG Jiao-jiao, LI Dan, SONG Jian, LIU Yan-ping, CHANG Ya-qing*, WANG Yi-nan*
    2015, 17(2):  134-140.  DOI: 10.13304/j.nykjdb.2014.382
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    This paper analyzed the bacteria community composition and variation of seawater from culture ponds of Apostichopus japonicus at Wafangdian, Dalian City, Liaoning Province. Seawater of different periods from culture ponds of Apostichopus japonicus was collected, and variable region fragments of 16S rDNA V3 were amplified by specific primers of bacteria, and analyzed with deformation gradient gel electrophoresis (DGGE). The main bands were cut, recovered, and sequenced. The results showed that the diversity index of samples from different periods varied from 1.994 5 to 2.230 5, and there were not big differences in evenness. The sample richness was between 42 and 48. But there were differences between bacterial community compositions of those samples. In March and September seawater shared the highest similarity(80%), and in June and other months, there was the biggest difference in cluster distance. Results of 39 main bands of sequences indicated that 12 bands of them were close to uncultured bacteria, 1 band was close to Actinobacteridae, and 26 bands were identified as Flavobacteria, α-proteobacteria and γ-proteobacteria. Flavobacteria, α-proteobacteria and γ-proteobacteria appeared in seawater at all periods, and among them Flavobacteria was dominant with proportion of 23.8%~50.0%. Seawater bacterial community compositions in cultured ponds at different periods were relatively stable, but there were differences in specific strains.

    Phospholipid Profiling Method and Progress on its Application Research
    WANG Xiang, WEI Fang*, LV Xin, DONG Xu-yan, CHEN Hong*
    2015, 17(2):  141-150.  DOI: 10.13304/j.nykjdb.2014.678
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    Phospholipid is a main component of all living cell membranes, and has important structural and functional features. Phospholipid high efficient profiling is helpful for deeper understanding the role of particular phospholipid played in maintaining healthy or sick state of a cell; and it also provides a technology platform for in-depth study on the biological and nutritional characteristics of phospholipid. Analyzing phospholipid has become more difficult, because of its complex structure and species in nature and in vivo. With the development of analyzing technology, many studies are carried out on the analyzing methods. This paper summarized the structure and biological function of phospholipid, methods for its extraction, separation, mass spectrum identification, quantitative analysis, and application in lipidomics. The development direction of phospholipid analisis was prospected, so as to provide references for establishing new phospholipid analyzing method, and for further studying its biological and nutritional characteristics.

    Research Progress of Aptamer in Detecting Harmful Substances in Food
    LU Jun-li1,2, GE Wei-dong3, SUN Jian-xia4*, YANG Yong2, JIAO Rui1, OU Shi-yi1, B
    2015, 17(2):  151-158.  DOI: 10.13304/j.nykjdb.2014.588
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    Aptamer is an oligonucleotide that can recognize specificity and bind to corresponding targets. It has strong affinity and is highly stable and easier to modify. It has been widely used in detection, separation and purification, medical treatment. The detection assay based on nucleic acid aptamer technology has developed rapidly, and been applied in detecting small molecule, protein, bacteria and viruses, etc.. This paper reviewed the research progress in detecting harmful substances in food by nucleic acid aptamer.

    Studies on Protected Vegetable Production Efficiency in China
    WANG Ya-kun1, WANG Hui-jun1,2*
    2015, 17(2):  159-166.  DOI: 10.13304/j.nykjdb.2015.019
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    As one of the major path to realize agricultural modernization, protected vegetables have been rapidly developed in China due to its good economic and social benefits. In order to comprehend the national production efficiency of protected vegetables, figure out the reasons of high or low production efficiency at different regions, and realize the macroeconomic regulation and control of vegetable production resources allocation, we adopted super efficiency DEA and Malmquist index method to ecalculate from static and dynamic perspective the efficiency of protected vegetables production in China. The results were obtained as follows: ① There were 23 DEA efficiency among all 63 DMUs in 2011-2013, when the DEA inefficiency proportion was 63.49% and problem about the excessive investments on protected vegetables was serious. ② Protected vegetables TFP(total factor productivity) in China increased by 0.8% in 2011-2013, technical efficiency was the power of TFP growth, technological advances had the negative effect on TFP growth. In order to improve the production efficiency of protected vegetables, the scientific research and achievement transformation of protected vegetables should be strengthened, the agricultural technology extension should be further promoted, and attention should be paid to learning and communication, so as to improve the production efficiency of protected vegetables.

    Research Progress on Economic Impacts of Animal Disease
    HUANG Ze-ying, WANG Ji-min*
    2015, 17(2):  167-173.  DOI: 10.13304/j.nykjdb.2014.690
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    Animal diseases have excerted bad influence on farmers, consumers, domestic and animal industries. Study the economic impact of animal disease is of great importance to evaluate the extent of loss, and develop effective prevention measures. Current studies mainly involved the economic impact of animal disease on producers, consumers, animal industries, international trade, and regional economy. But studies about its influence on international trade and regional economy were less, and application of inferential statistics is not much. Compared with the international society, studies in China is lag behind. So future research should lay emphasis on influence of animal diseases to consumers, animal industries, nation and the world. We should also break through the present partial, static, theoraticle research method, and try to adhere to inter-temporal and general equilibrium theories in accordance with animal disease characteristics; and apply econometric models including spatial econometrics and epidemiology; and also pay attention to simulate and forecast economic impacts of animal diseases.

    Problems in Agricultural Informationization Investment and Countermeasure ——Taking Chongqing as an Example
    ZHANG Jian-hua, WU Jian-zhai*, WANG Sheng-wei, LIU Jia-jia, ZHU Meng-shuai, ZHAO
    2015, 17(2):  174-181.  DOI: 10.13304/j.nykjdb.2014.413
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    Informationization is an important character of modern agriculture. It is not only a key driving force for sustainable agriculture development in China, but also a strategic measure for accelerating agriculture modernization in China. Taking Chongqing as an example, the paper cited the basic situation of the city in recent years, including agricultural information policy support, fund input, project application and social participation. This paper also analyzed the existing problems in agricultural informationization investment direction, mechanism and effects. On this basis, the paper studied the countermeasures for future agricultural informatization development from investment perspective, and put forward suggestions for perfecting agricultural informatization investment direction, mechanism, and improving the effectiveness of agricultural informatization investment. All these were expected to provide references for formulating relevant policies for agricultural informatization.