电子加速器诱变育种材料进展初报

doi:10.13304/j.nykjdb.2024.0726

中国农业科技导报 ›› 2024, Vol. 26 ›› Issue (12): 210-216.DOI: 10.13304/j.nykjdb.2024.0726

• 方法与技术创新 • 上一篇

电子加速器诱变育种材料进展初报

翟虎渠¹(), 曹兵²(), 顾爽², 夏志强², 夏志辉², 龚家建², 尹航³, 李立⁴, 李卫⁴, 任杰⁴

^1.中国农业科学院，北京 100081
^2.海南大学南繁学院（三亚南繁研究院），海南三亚 572025
^3.淮阴农业科学研究所，江苏淮安 223001
^4.三亚鸣遥种质创新科技有限公司，海南三亚 572000

收稿日期:2024-09-04 接受日期:2024-10-30 出版日期:2024-12-15 发布日期:2024-12-17
通讯作者: 曹兵
作者简介:翟虎渠 E-mail：maxiao@caas.cn；
基金资助:
热带作物生物育种全国重点实验室科研专项(NKLTCB202338)

Preliminary Report on Breeding Materials Induced by Electron Accelerator

Huqu ZHAI¹(), Bing CAO²(), Shuang GU², Zhiqiang XIA², Zhihui XIA², Jiajian GONG², Hang YIN³, Li LI⁴, Wei LI⁴, Jie REN⁴

^1.Chinese Academy of Agricultural Sciences，Beijing 100081，China
^2.Sanya Indtitute of Southern Breeding，Hainan University，Hainan Sanya 572025，China
^3.Huaiyin Institute of Agricultural Sciences，Jiangsu Huaian 223001，China
^4.Sanya Mingyao Germplasm Innovation Technology CO. ，Ltd. ，Hainan Sanya 572000，China

Received:2024-09-04 Accepted:2024-10-30 Online:2024-12-15 Published:2024-12-17
Contact: Bing CAO

摘要/Abstract

摘要：

种质创新是育种的关键，利用电子加速器产生的X射线作为诱变工具，探索并优化这一技术在育种材料中的应用策略。首先，通过精细调控确定了针对不同处理对象的半致死/半致矮剂量阈值，随后逐步微调辐照强度，旨在诱发遗传变异的同时保持处理对象的存活率。进一步探讨了处理材料的最佳处理时机与方式，以期最大化辐照诱导变异的效率。针对多种作物实施了辐照处理，初步成效显著。玉米植株在辐照后表现出显著的株高矮化现象，且矮化程度与处理强度呈正相关；大豆辐照后不仅株高降低，还展现出抗虫性增强，表明辐照诱导了有利性状的产生；藜麦在辐照后株高、叶色、花色及穗形等多个性状上展现出广泛的遗传变异；通过特定辐照处理并筛选，成功培育出具有显著抗除草剂特性的水稻植株，为杂草管理提供了新策略。以上研究结果不仅丰富了诱变育种的理论体系，也为作物遗传改良与品种创新开辟了新途径。

关键词: 电子加速器, 辐照诱变, 半致死剂量, 育种

Abstract:

Germplasm innovation is the cornerstone of breeding， so the X-rays generated by electron accelerators was as a mutagenic tool to explore and optimize the application strategies of breeding materials. Initially， through meticulous adjustments， the semi-lethal/semi-dwarf dose thresholds were established for diverse treatment subjects. Subsequently， fine-tuning of irradiation intensities was performed to elicit genetic variations， and the treated materials maintained high survival rates. Furthermore， the optimal timing and approach for material treatment were explored to maximize the efficiency of irradiation-induced mutations. The irradiation treatments were implemented on a wide range of crops， achieving notable preliminary successes. Maize plants exhibited pronounced dwarfing after irradiation， and the degree of dwarfing was positively correlated with the treatment intensity. After irradiation， soybean plants exhibited reduced plant height and enhanced insect resistance， indicating the induction of favorable traits through irradiation. Quinoa treated by irradiation manifested extensive genetic variations of multiple traits， including plant height， leaf color， flower color and panicle morphology. Through specific irradiation treatments and screening， rice plants with significant herbicide resistance were successfully bred， offering a novel strategy for weed management. Above results not only enriched the theoretical framework of mutagenic breeding， but also opened up new avenues for crop genetic improvement and variety innovation.

Key words: electron accelerator, irradiation mutagenesis, semi-lethal dose, breeding

中图分类号:

Q319+.33

翟虎渠, 曹兵, 顾爽, 夏志强, 夏志辉, 龚家建, 尹航, 李立, 李卫, 任杰. 电子加速器诱变育种材料进展初报[J]. 中国农业科技导报, 2024, 26(12): 210-216.

Huqu ZHAI, Bing CAO, Shuang GU, Zhiqiang XIA, Zhihui XIA, Jiajian GONG, Hang YIN, Li LI, Wei LI, Jie REN. Preliminary Report on Breeding Materials Induced by Electron Accelerator[J]. Journal of Agricultural Science and Technology, 2024, 26(12): 210-216.

图/表 10

图 1 玉米浸泡后的种子含水量

Fig. 1 Water content of maize seed after soaking

图2 不同辐照处理下玉米幼苗的表型及株高注：**表示在P<0.01水平差异显著。

Fig. 2 Phenotypes and plant height of maize seedlings under different irradiation treatmentsNote： ** indicates significant difference at P<0.01 level.

图3 30 Gy处理玉米株系全基因组突变密度图注：a—GC含量；b—基因数量；c—SNP数量；d—插入数量；e—删除数量；INS—插入；DEL—缺失。

Fig. 3 Whole genome mutation density map of maize line treated with 30 GyNote： a—GC content； b—Number of genes； c—Number of SNPs； d—Number of insertions； e—Number of deletions； INS—Insert； DEL—Delete.

图 4 大豆浸泡后含水量曲线

Fig. 4 Water content curve of soybean after soaking

图5 不同辐照处理下大豆的表型及株高注：**表示在P<0.01水平差异显著。

Fig. 5 Phenotypes and plant height of soybean under different irradiation treatmentsNote： ** indicates significant at P<0.01 level.

图6 不同辐照处理下黑豆81的SNP变异位点A：40 Gy； B：60 Gy； C：80 Gy

Fig. 6 SNP variation sites of Black Bean 81 under different radiation treatments

图7 辐照处理后大豆苗期黄化突变株

Fig. 7 Soybean yellowing mutant at seedling stage after irradiation treatment

图8 辐照处理后M1代淮藜3号突变位点富集分析注：a—GC含量；b—基因数量；c—SNP数量；d—插入数量；e—删除数量；INS—插入；DEL—缺失。

Fig. 8 Enrichment analysis of mutation site of Huaili 3 M1 generation after irradiationNote： a—GC content； b—Number of genes； c—Number of SNPs； d—Number of insertions； e—Number of deletions； INS—Insert； DEL—Delete.

图9 部分藜麦辐照突变体的表型

Fig. 9 Phenotype of some quinoa irradiated mutants

图10 辐照处理黄华占的M1代喷洒除草剂后的表现

Fig. 10 Performance of irradiated Huanghuazhan M1 generation after herbicide spraying

参考文献 11

1	刘志宏,田媛,陈红娜,等.水稻转基因育种的研究进展与应用现状[J].中国种业,2023(9):11-17.
	LIU Z H, TIAN Y, CHEN H N,et al..Research progress and application status of rice transgenic breeding [J].China Seed Ind., 2023(9):11-17.
2	杨大兵,胡亮,杜雪树,等.CRISPR/Cas9基因编辑技术创制水稻雄性不育系的研究进展[J/OL].中国农业科技导报,2024:1-11[2024-08-20]..
	YANG D B, HU L, DU X S, et al.. Progress in creation of rice male sterile lines by CRISPR/Cas9 gene editing technology [J/OL]. J. Agric. Sci. Technol., 2024:1-11 [2024-08-20]. .
3	金瑾.生物育种对中国粮食安全的贡献和挑战[J].分子植物育种,2024,22(17):5911-5916.
	JIN J. The contribution and challenges of biobreeding to China’s food security [J]. Mol. Breed., 2024,22(17): 5911-5916.
4	刘录祥,郭会君,赵林姝,等.植物诱发突变技术育种研究现状与展望[J].核农学报, 2009,23(6):1001-1007.
	LIU L X, GUO H J, ZHAO L S, et al.. Current status and outlook perspectives of induced mutations for plant improvement [J]. J. Nucl. Agric. Sci., 2009, 23(6):1001-1007.
5	MA L Q, KONG F Q, SUN K, et al.. From classical radiation to modern radiation:past,present,and future of radiation mutation breeding [J/OL]. Front.Public Health,2021,9:768071 [2024-08-20]. .
6	顾爽,曹兵,王鹿,等.利用热带生态的特殊性开展辐射诱变育种条件分析与展望[J].种子,2024,43(5):64-69.
	GU S, CAO B, WANG L, et al.. Analysis and prospect of radiation induced breeding conditions utilizing the special characteristics of tropical ecology [J]. Seed, 2024, 43(5):64-69.
7	刘蕴韬,安世忠,梁积新.核技术应用现状及发展趋势[J].科技导报,2022,40(11):88-97.
	LIU Y T, AN S Z, LIANG J X. Current status and prospect of nuclear technology and application [J].Sci. Technol. Rev., 2022,40(11):88-97.
8	张子涛,张进.一种全自动电子加速器生物辐照育种诱变装置:CN116616172A[P].2023-08-22.
9	瞿建国,李立,李宇轩.一种安全无污染的种子辐照装置:CN220308041U[P].2024-01-09.
10	瞿建国,李立,李宇轩.一种通过电子束轰击金属靶产生X射线的装置:CN219811459U[P].2023-10-10.
11	黄桂丹. ⁶⁰Co-γ射线辐射育种研究进展[J].林业与环境科学,2016,32(2):107-111.
	HUANG G D. Research progress for ⁶⁰Co-γ ray radiation breeding [J]. For. Environ. Sci., 2016, 32(2):107-111.

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电子加速器诱变育种材料进展初报

Preliminary Report on Breeding Materials Induced by Electron Accelerator

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