减氮配施聚天冬氨酸对烤烟氮素利用及产质量的影响

doi:10.13304/j.nykjdb.2023.0553

中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (3): 227-238.DOI: 10.13304/j.nykjdb.2023.0553

• 生物制造资源生态 • 上一篇

减氮配施聚天冬氨酸对烤烟氮素利用及产质量的影响

段俊雅¹(), 赵园园¹(), 王婷婷¹, 韦建玉², 王政², 王德勋³, 李娟³, 史宏志¹()

^1.河南农业大学烟草学院，烟草农业减害研究中心，烟草行业烟草栽培重点实验室，郑州 450046
^2.广西中烟工业有限责任公司，南宁 530001
^3.云南省烟草公司大理州公司，云南大理 671000

收稿日期:2023-07-18 接受日期:2023-09-12 出版日期:2025-03-15 发布日期:2025-03-14
通讯作者: 赵园园,史宏志
作者简介:段俊雅 E-mail：15713888578@163.com
基金资助:
广西中烟工业有限责任公司项目(2021450000340021);中国烟草总公司云南省公司科技项目(2022530000241015)

Effects of Nitrogen Reduction Combined with Polyaspartic Acid on Nitrogen Utilization， Yield and Quality of Flue-cured Tobacco

Junya DUAN¹(), Yuanyuan ZHAO¹(), Tingting WANG¹, Jianyu WEI², Zheng WANG², Dexun WANG³, Juan LI³, Hongzhi SHI¹()

^1.Tobacco Cultivation Key Laboratory of China Tobacco，Research Center for Tobacco Harm Reduction，College of Tobacco Science，Henan Agricultural University，Zhengzhou 450046，China
^2.China Tobacco Guangxi Industrial Co. ，Ltd. ，Nanning 530001，China
^3.Dali Branch of Yunnan Provincial Tobacco Company，Yunnan Dali 671000，China

Received:2023-07-18 Accepted:2023-09-12 Online:2025-03-15 Published:2025-03-14
Contact: Yuanyuan ZHAO,Hongzhi SHI

摘要/Abstract

摘要：

为明确烤烟生产上施用聚天冬氨酸（polyaspartic acid，PASP）对减施氮肥条件下的增效提质作用，以‘红花大金元’为供试材料进行裂区试验，设置常规施氮（N1）及在常规施氮的基础上减氮10%（N2）、15%（N3）、20%（N4）4个施氮量处理，同时分别配施0.0%（P1）、2.5%（P2）、5.0%（P3）、7.5%（P4）的PASP，研究减氮条件下施用PASP对烤烟氮素利用及产质量的影响。结果表明，随着施氮量的减少，烟株生长发育变弱，叶片SPAD值和氮素吸收积累量降低，产量减少，氮肥表观利用率下降，生理利用率升高，烤后叶片糖含量升高，烟碱和总氮含量降低，劲头变小。等氮条件下，随着配施PASP比例的增加，烟株农艺指标明显改善，与不添加PASP相比，P4处理的SPAD值提高10.08%~18.44%，产量增加7.54%~9.44%，氮肥表观利用率和农学效率分别提高56.21%~72.59%和21.61%~28.38%，减氮条件下添加PASP烤后烟叶的吸食品质更好。综合来看，减氮15%条件下配施5.0% PASP处理烤后烟叶的产量和产值与常规施氮不添加PASP处理相当，同时氮肥利用率提高，烤烟质量较优。

关键词: 烤烟, 减氮, 聚天冬氨酸（PASP）, 氮肥利用率, 产质量

Abstract:

In order to determine the effect of polyaspartic acid （PASP） on the efficiency and quality of flue-cured tobacco production under the condition of reduced nitrogen application， a split-plot experiment was conducted with ‘Honghua Dajinyuan’ as the test material. 4 nitrogen application treatments were set up， including the conventional nitrogen application （N1） and nitrogen reduction rates of 10% （N2）， 15% （N3） and 20% （N4） on the basis of conventional nitrogen application. At the same time， 0.0% （P1）， 2.5% （P2）， 5.0% （P3） and 7.5% （P4） PASP were applied， respectively. The effects of PASP on nitrogen utilization， yield and quality of flue-cured tobacco under nitrogen reduction were studied. The results showed that， with the decrease of nitrogen application rate， the growth and development of tobacco plants became weaker， the SPAD value， nitrogen absorption and accumulation， yield and nitrogen apparent utilization decreased， the nitrogen physiological utilization increased， the sugar content of flue-cured tobacco leaves increased， the nicotine and total nitrogen content decreased， and the smoking power became smaller. Under same nitrogen conditions， with the increase of PASP ratio， the agronomic indexes of tobacco plants were significantly improved. Compared with no PASP， SPAD value and yield of P4 treatment increased by 10.08%~18.44% and 7.54%~9.44%， respectively， and the apparent utilization rate and agronomic utilization rate of nitrogen fertilizer were increased by 56.21%~72.59% and 21.61%~28.38%， respectively. The smoking quality of tobacco leaves of PASP treatments under nitrogen reduction condition was better. In conclusion， the yield and output value of flue-cured tobacco leaves treated with 5.0% PASP under the condition of 15% nitrogen reduction were similar to the conventional nitrogen application without adding PASP treatment， and the nitrogen utilization rate increased， the quality of flue-cured tobacco was better.

Key words: flue-cured tobacco, reducing nitrogen application, polyaspartic acid （PASP）, nitrogen use efficiency, yield and quality

中图分类号:

S572

段俊雅, 赵园园, 王婷婷, 韦建玉, 王政, 王德勋, 李娟, 史宏志. 减氮配施聚天冬氨酸对烤烟氮素利用及产质量的影响[J]. 中国农业科技导报, 2025, 27(3): 227-238.

Junya DUAN, Yuanyuan ZHAO, Tingting WANG, Jianyu WEI, Zheng WANG, Dexun WANG, Juan LI, Hongzhi SHI. Effects of Nitrogen Reduction Combined with Polyaspartic Acid on Nitrogen Utilization， Yield and Quality of Flue-cured Tobacco[J]. Journal of Agricultural Science and Technology, 2025, 27(3): 227-238.

图/表 8

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处理 Treatment		施氮量 Nitrogen application rate/（kg·hm^-2）	PASP用量 PASP application rate/（g·hm^-2）
N1	P1	75.00	0.00
	P2		1 875.00
	P3		3 750.00
	P4		5 625.00
N2	P1	67.50	0.00
	P2		1 687.50
	P3		3 375.00
	P4		5 062.50
N3	P1	63.75	0.00
	P2		1 593.75
	P3		3 187.50
	P4		4 781.25
N4	P1	60.00	0.00
	P2		1 500.00
	P3		3 000.00
	P4		4 500.00

处理 Treatment		施氮量 Nitrogen application rate/（kg·hm^-2）	PASP用量 PASP application rate/（g·hm^-2）
N1	P1	75.00	0.00
	P2		1 875.00
	P3		3 750.00
	P4		5 625.00
N2	P1	67.50	0.00
	P2		1 687.50
	P3		3 375.00
	P4		5 062.50
N3	P1	63.75	0.00
	P2		1 593.75
	P3		3 187.50
	P4		4 781.25
N4	P1	60.00	0.00
	P2		1 500.00
	P3		3 000.00
	P4		4 500.00

处理Treatment		株高 Plant height	茎围 Stem girth	节距 Pitch	最大叶长 Maximum leaf length	最大叶宽 Maximum leaf width
N1	P1	59.73±1.97 g	8.97±0.12 cde	4.51±0.14 efg	64.13±0.82 efg	32.13±0.70 cdefg
	P2	64.60±1.37 de	9.20±0.08 abc	4.60±0.01 def	65.77±0.17 cde	32.47±0.26 bcdef
	P3	69.33±0.94 bc	9.37±0.12 ab	4.89±0.09 bc	68.27±2.88 abc	33.33±0.29 bc
	P4	72.67±0.47 a	9.50±0.14 a	5.34±0.08 a	70.77±0.54 a	35.13±0.76 a
	均值Mean	66.58	9.26	4.84	67.24	33.27
N2	P1	57.33±1.25 h	8.50±0.08 fg	4.31±0.13 g	62.83±0.59 efgh	30.97±0.73 efgh
	P2	62.17±1.31 f	8.77±0.31 defg	4.50±0.05 fg	64.70±0.91 def	31.10±0.22 efgh
	P3	68.17±1.03 c	9.23±0.21 abc	4.76±0.09 bcd	67.17±1.43 bcd	32.60±0.85 bcde
	P4	70.67±1.25 ab	9.30±0.08 abc	4.93±0.07 b	68.83±0.62 ab	33.80±0.91 ab
	均值Mean	64.59	8.95	4.63	65.88	32.12
N3	P1	54.33±0.47 i	8.47±0.17 g	4.03±0.08 h	61.23±0.61 gh	30.50±0.57 gh
	P2	60.67±0.47 fg	8.73±0.21 defg	4.40±0.05 fg	63.90±0.42 efg	30.90±0.29 fgh
	P3	65.17±0.62 d	9.03±0.12 bcd	4.60±0.15 def	65.27±2.30 cdef	32.40±1.13 bcdef
	P4	69.67±1.25 bc	9.27±0.05 abc	4.85±0.03 bc	67.67±1.20 bcd	32.80±0.51 bcd
	均值Mean	62.46	8.88	4.47	64.52	31.65
N4	P1	51.63±0.85 j	8.43±0.17 g	3.87±0.13 h	60.03±0.99 h	28.33±0.97 i
	P2	59.67±1.25 g	8.67±0.05 efg	4.31±0.07 g	62.50±0.93 fgh	30.10±1.10 h
	P3	62.67±0.47 ef	8.83±0.12 def	4.54±0.03 ef	64.07±1.92 efg	31.57±0.37 defgh
	P4	68.00±0.82 c	9.23±0.05 abc	4.71±0.10 cde	65.90±0.71 bcde	32.10±0.37 cdefg
	均值Mean	60.49	8.79	4.36	63.13	30.53
	N	**	**	**	**	**
	P	**	**	**	**	**
	N×P	*	ns	ns	ns	ns

处理Treatment		株高 Plant height	茎围 Stem girth	节距 Pitch	最大叶长 Maximum leaf length	最大叶宽 Maximum leaf width
N1	P1	59.73±1.97 g	8.97±0.12 cde	4.51±0.14 efg	64.13±0.82 efg	32.13±0.70 cdefg
	P2	64.60±1.37 de	9.20±0.08 abc	4.60±0.01 def	65.77±0.17 cde	32.47±0.26 bcdef
	P3	69.33±0.94 bc	9.37±0.12 ab	4.89±0.09 bc	68.27±2.88 abc	33.33±0.29 bc
	P4	72.67±0.47 a	9.50±0.14 a	5.34±0.08 a	70.77±0.54 a	35.13±0.76 a
	均值Mean	66.58	9.26	4.84	67.24	33.27
N2	P1	57.33±1.25 h	8.50±0.08 fg	4.31±0.13 g	62.83±0.59 efgh	30.97±0.73 efgh
	P2	62.17±1.31 f	8.77±0.31 defg	4.50±0.05 fg	64.70±0.91 def	31.10±0.22 efgh
	P3	68.17±1.03 c	9.23±0.21 abc	4.76±0.09 bcd	67.17±1.43 bcd	32.60±0.85 bcde
	P4	70.67±1.25 ab	9.30±0.08 abc	4.93±0.07 b	68.83±0.62 ab	33.80±0.91 ab
	均值Mean	64.59	8.95	4.63	65.88	32.12
N3	P1	54.33±0.47 i	8.47±0.17 g	4.03±0.08 h	61.23±0.61 gh	30.50±0.57 gh
	P2	60.67±0.47 fg	8.73±0.21 defg	4.40±0.05 fg	63.90±0.42 efg	30.90±0.29 fgh
	P3	65.17±0.62 d	9.03±0.12 bcd	4.60±0.15 def	65.27±2.30 cdef	32.40±1.13 bcdef
	P4	69.67±1.25 bc	9.27±0.05 abc	4.85±0.03 bc	67.67±1.20 bcd	32.80±0.51 bcd
	均值Mean	62.46	8.88	4.47	64.52	31.65
N4	P1	51.63±0.85 j	8.43±0.17 g	3.87±0.13 h	60.03±0.99 h	28.33±0.97 i
	P2	59.67±1.25 g	8.67±0.05 efg	4.31±0.07 g	62.50±0.93 fgh	30.10±1.10 h
	P3	62.67±0.47 ef	8.83±0.12 def	4.54±0.03 ef	64.07±1.92 efg	31.57±0.37 defgh
	P4	68.00±0.82 c	9.23±0.05 abc	4.71±0.10 cde	65.90±0.71 bcde	32.10±0.37 cdefg
	均值Mean	60.49	8.79	4.36	63.13	30.53
	N	**	**	**	**	**
	P	**	**	**	**	**
	N×P	*	ns	ns	ns	ns

处理 Treatment		产量 Yield/（kg·hm^-2）	均价/（元·kg^-1） Mean price/（yuan·kg^-1）	产值/（元·hm^-2） Output value/（yuan·hm^-2）	中上等烟比例 Proportion of medium and high quality tobacco/%
N1	P1	2 027.70±28.29 efg	39.19±0.71 efg	79 465.50±640.79 g	93.81±1.45 e
	P2	2 110.05±32.70 cd	40.55±0.57 a	85 562.55±638.83 c	95.60±0.63 a
	P3	2 188.05±89.90 ab	40.25±0.79 ab	88 069.05±672.51 a	95.43±1.37 a
	P4	2 219.10±42.38 a	39.36±0.42 def	87 343.80±645.56 b	93.98±0.86 de
	均值Mean	2 136.23	39.84	85 110.23	94.71
N2	P1	1 985.40±32.46 fgh	37.90±0.69 i	75 246.60±552.36 i	92.27±0.72 g
	P2	2 075.25±32.46 de	38.73±0.32 gh	80 374.50±412.62 f	92.78±1.40 g
	P3	2 102.25±23.64 cd	39.96±0.29 bc	84 005.85±682.18 d	94.75±1.19 bc
	P4	2 146.80±36.62 bc	39.82±0.19 bcd	85 485.60±1 216.91 c	94.64±1.36 bcd
	均值Mean	2 077.43	39.10	81 278.14	93.61
N3	P1	1 906.80±26.33 ij	37.75±0.15 i	71 981.70±685.24 k	92.21±1.12 g
	P2	1 988.10±23.39 fgh	38.61±0.36 h	76 760.55±700.68 h	92.40±1.05 g
	P3	2 014.80±15.06 efg	39.54±0.25 cde	79 665.15±993.76 g	94.13±1.11 cde
	P4	2 050.50±27.56 def	40.17±0.73 ab	82 368.60±1 077.29 e	95.13±1.61 ab
	均值Mean	1 990.05	39.02	77 694.00	93.47
N4	P1	1 808.70±31.23 k	37.72±0.16 i	68 224.20±834.17 l	92.18±1.54 g
	P2	1 901.10±38.58 j	38.59±0.81 h	73 363.50±618.13 j	92.30±1.18 g
	P3	1 944.45±28.29 hij	38.75±0.20 gh	75 347.40±641.64 i	92.84±0.66 fg
	P4	1 969.65±25.84 ghi	38.82±0.76 fgh	76 461.75±1 040.67 h	93.45±1.37 ef
	均值Mean	1 905.98	38.47	73 349.21	92.69
	N	**	**	**	**
	P	**	**	**	**
	N×P	ns	**	**	**

减氮配施聚天冬氨酸对烤烟氮素利用及产质量的影响

Effects of Nitrogen Reduction Combined with Polyaspartic Acid on Nitrogen Utilization， Yield and Quality of Flue-cured Tobacco

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Metrics

处理 Treatment		烟株氮素积累量 Nitrogen accumulation in tobacco plants/（kg·hm^-2）	氮肥表观利用率 Nitrogen apparent efficiency/%	氮肥农学效率 Nitrogen agronomic efficiency/（kg·kg^-1）	氮肥生理利用率 Nitrogen physiological efficiency/%
N1	P1	85.19±2.92 d	37.04±1.51 f	10.51±0.29 g	28.36±0.22 i
	P2	90.56±2.42 c	44.20±0.89 d	11.60±0.10 e	26.25±0.99 j
	P3	95.93±2.02 b	51.36±0.90 b	12.64±0.03 c	24.62±0.35 k
	P4	101.11±1.98 a	58.27±1.98 a	13.06±0.22 b	22.41±0.33 l
	均值Mean	93.20	47.72	11.95	25.41
N2	P1	77.22±1.15 h	29.35±1.00 i	11.05±0.30 f	37.64±0.55 d
	P2	82.41±0.48 f	37.04±1.46 f	12.38±0.22 d	33.42±1.29 g
	P3	84.63±0.10 de	40.33±1.49 e	12.78±0.26 c	31.69±0.74 h
	P4	90.19±1.04 c	48.56±1.41 c	13.44±0.29 a	27.67±1.02 i
	均值Mean	83.61	38.82	12.41	32.61
N3	P1	73.89±1.01 i	25.85±0.29 j	10.46±0.33 g	40.48±1.27 b
	P2	77.78±0.07 h	31.95±1.06 h	11.74±0.02 e	36.74±0.26 e
	P3	81.85±0.13 fg	38.34±0.25 f	12.16±0.33 d	31.71±1.01 h
	P4	83.15±2.38 ef	40.38±0.27 e	12.72±0.14 c	31.50±0.92 h
	均值Mean	79.17	34.13	11.77	35.11
N4	P1	70.61±1.54 j	22.00±0.07 k	9.48±0.04 h	43.10±0.82 a
	P2	74.42±1.87 i	28.35±0.91 i	11.02±0.30 f	38.88±0.12 c
	P3	77.63±2.24 h	33.70±0.64 g	11.75±0.30 e	34.85±0.81 f
	P4	80.19±1.50 g	37.97±0.58 f	12.17±0.25 d	32.04±0.32 h
	均值Mean	75.71	30.51	11.11	37.22
	N	**	**	**	**
	P	**	**	**	**
	N×P	**	**	**	**

处理Treatment		叶长 Leaf length/cm	叶宽 Leaf width/cm	单叶重 Single leaf weight/g	叶质重 Leaf density/（g·m^-2）	含梗率 Midrib ratio/%
N1	P1	64.32±0.41 cd	26.27±0.21 def	14.34±0.25 bcd	99.04±2.13 bc	27.27±1.44 h
	P2	65.17±0.62 bc	27.17±0.39 abc	14.86±0.37 bc	99.99±3.62 bc	26.83±1.23 hi
	P3	66.16±0.41 b	27.27±0.40 ab	15.02±0.36 b	101.29±1.58 b	26.75±0.79 hi
	P4	67.37±0.74 a	27.47±0.48 a	15.94±0.42 a	106.76±0.68 a	26.01±0.59 i
	均值Mean	65.76	27.05	15.04	101.77	26.71
N2	P1	62.62±0.29 ef	25.60±0.36 fgh	13.38±0.41 efg	92.16±0.20 d	28.74±1.11 efg
	P2	64.03±0.41 cd	26.13±0.26 efg	13.63±0.38 def	96.13±2.56 c	28.40±0.13 fg
	P3	65.05±0.70 bc	26.53±0.31 bcde	13.98±0.33 de	98.50±2.85 bc	28.23±0.39 g
	P4	65.83±0.68 b	26.90±0.30 abcd	14.22±0.30 cd	99.49±1.65 bc	28.11±1.93 g
	均值Mean	64.38	26.29	13.80	96.57	28.37
N3	P1	61.15±0.40 gh	25.03±0.25 hi	12.55±0.22 hi	83.80±0.09 e	29.77±1.33 abcd
	P2	63.03±0.41 de	25.48±0.33 gh	13.00±0.45 fgh	84.70±0.24 e	29.67±1.10 bcd
	P3	63.83±0.32 cde	25.77±0.34 efgh	13.25±0.36 efgh	88.57±2.23 d	29.45±0.30 cde
	P4	64.45±0.41 c	26.41±0.35 cde	13.56±0.29 defg	89.66±1.87 d	29.12±0.22 def
	均值Mean	63.12	25.67	13.09	86.68	29.50
N4	P1	60.03±0.90 h	21.47±0.18 l	10.94±0.19 j	76.17±0.36 g	30.57±1.35 a
	P2	61.60±0.54 fg	23.73±0.31 k	12.10±0.40 i	78.94±1.31 fg	30.50±1.00 ab
	P3	63.00±0.81 de	23.87±0.33 jk	12.13±0.20 i	79.50±3.01 fg	30.42±0.47 ab
	P4	64.26±0.83 cd	24.53±0.12 ij	12.78±0.38 ghi	82.17±0.88 ef	30.26±1.27 abc
	均值Mean	62.22	23.40	11.99	79.20	30.44
	N	**	**	**	**	**
	P	**	**	**	**	**
	N×P	ns	*	ns	ns	ns

处理Treatment		还原糖 Reducig sugar/%	总糖 Total sugar/%	烟碱 Nicotine/%	总氮 Total nitrogen/%	糖碱比 Sugar-nicotine ratio	氮碱比 Nitrogen-nicotine ratio
N1	P1	29.30±0.56 g	38.00±0.33 d	2.06±0.01 f	1.85±0.05 de	18.45±0.08 e	0.90±0.02 cd
	P2	28.40±0.57 h	36.70±0.76 e	2.23±0.05 e	1.86±0.01 d	16.46±0.02 f	0.83±0.01 efgh
	P3	25.30±0.63 l	34.80±0.36 f	2.69±0.02 a	2.17±0.03 a	12.94±0.02 g	0.81±0.00 fh
	P4	23.90±0.43 m	31.40±0.33 i	2.38±0.01 c	1.99±0.03 c	13.19±0.14 g	0.84±0.02 efgh
	均值Mean	26.73	35.23	2.34	1.97	15.26	0.85
N2	P1	30.90±0.16 e	40.40±0.47 b	1.82±0.05 h	1.69±0.02 g	22.42±0.51 c	0.93±0.02 c
	P2	29.60±0.63 fg	39.10±0.03 c	2.08±0.01 f	1.73±0.06 f	19.57±0.69 de	0.83±0.02 efgh
	P3	26.90±0.34 j	33.70±0.51 g	2.51±0.07 b	2.12±0.03 b	15.18±1.62 f	0.84±0.01 efg
	P4	26.40±0.69 k	33.30±0.36 h	2.32±0.04 d	1.96±0.04 c	16.34±1.71 f	0.84±0.00 ef
	均值Mean	28.45	36.63	2.18	1.88	18.38	0.86
N3	P1	32.00±0.06 c	40.80±0.07 b	1.78±0.05 h	1.59±0.06 h	22.70±0.39 c	0.89±0.01 d
	P2	31.60±0.52 d	40.70±0.46 b	1.91±0.07 g	1.69±0.01 g	20.47±0.13 d	0.89±0.04 d
	P3	29.80±0.42 f	38.10±0.51 d	2.19±0.05 e	1.86±0.03 d	15.39±1.49 f	0.85±0.00 e
	P4	27.80±0.27 i	37.90±0.11 d	2.20±0.02 e	1.82±0.03 e	15.14±1.57 f	0.83±0.01 efgh
	均值Mean	30.30	39.38	2.02	1.74	18.43	0.86
N4	P1	35.90±0.50 a	42.00±0.48 a	1.32±0.01 k	1.44±0.03 j	31.82±0.16 a	1.09±0.02 a
	P2	32.80±0.18 b	40.60±0.31 b	1.56±0.02 j	1.52±0.06 i	26.03±0.21 b	0.97±0.02 b
	P3	32.50±0.35 b	40.50±0.50 b	1.70±0.02 i	1.57±0.04 h	23.82±0.07 c	0.92±0.02 cd
	P4	30.00±0.65 f	38.10±0.19 d	1.87±0.02 g	1.66±0.03 g	20.37±0.16 d	0.89±0.01 d
	均值Mean	32.80	40.30	1.61	1.55	25.51	0.97
	N	**	**	**	**	**	**
	P	**	**	**	**	**	**
	N×P	**	**	**	**	**	**

处理Treatment		香气质 Aroma quality	香气量 Aroma quantity	透发性 Exposure degree	含量 Content	细腻性 Delicacy	杂气 Offensive odor	劲头 Stiffness	刺激性 Irritancy	余味 After taste	甜润感 Sweetness	总分 Total score
N1	P1	6.30	6.20	6.00	6.20	6.20	6.20	5.60	6.20	6.20	6.30	61.40
	P2	6.20	6.30	6.10	6.50	6.10	6.20	5.80	6.20	6.20	6.30	61.90
	P3	6.00	6.40	6.30	6.50	6.00	6.00	5.90	6.00	6.00	6.10	61.20
	P4	5.80	6.30	6.30	6.40	5.80	5.80	6.10	5.70	5.90	6.00	60.10
	均值Mean	6.08	6.30	6.18	6.40	6.03	6.05	5.85	6.03	6.08	6.18	61.15
N2	P1	6.30	6.20	6.00	6.30	6.20	6.30	5.50	6.30	6.30	6.40	61.80
	P2	6.50	6.30	6.10	6.30	6.20	6.30	5.60	6.30	6.40	6.40	62.40
	P3	6.50	6.50	6.20	6.50	6.10	6.30	5.70	6.30	6.40	6.40	62.90
	P4	6.30	6.50	6.30	6.50	6.00	6.20	5.70	6.20	6.30	6.30	62.30
	均值Mean	6.40	6.38	6.15	6.40	6.13	6.28	5.63	6.28	6.35	6.38	62.35
N3	P1	6.40	6.00	6.00	6.30	6.30	6.30	5.40	6.50	6.40	6.40	62.00
	P2	6.50	6.30	6.20	6.50	6.30	6.30	5.60	6.50	6.40	6.50	63.10
	P3	6.50	6.50	6.30	6.50	6.30	6.30	5.70	6.50	6.50	6.50	63.60
	P4	6.40	6.50	6.30	6.50	6.20	6.20	5.70	6.40	6.50	6.40	63.10
	均值Mean	6.45	6.33	6.20	6.45	6.28	6.28	5.60	6.48	6.45	6.45	62.95
N4	P1	6.20	5.80	5.90	6.00	6.00	6.20	5.30	6.50	6.00	6.10	60.00
	P2	6.40	6.10	6.00	6.20	6.10	6.20	5.50	6.40	6.20	6.30	61.40
	P3	6.40	6.20	6.20	6.30	6.10	6.20	5.60	6.40	6.20	6.30	61.90
	P4	6.30	6.20	6.20	6.30	6.00	6.00	5.60	6.30	6.10	6.20	61.20
	均值Mean	6.33	6.08	6.08	6.20	6.05	6.15	5.50	6.40	6.13	6.23	61.13

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