中国农业科技导报 ›› 2023, Vol. 25 ›› Issue (10): 54-64.DOI: 10.13304/j.nykjdb.2022.0936

• 生物技术 生命科学 • 上一篇    下一篇

烤烟种质资源叶片收缩及经济性状表型遗传多样性分析

吴兴富(), 焦芳婵, 陈学军, 冯智宇, 张光海, 张谊寒, 李永平()   

  1. 云南省烟草农业科学研究院,烟草行业烟草生物技术育种重点实验室,昆明 650021
  • 收稿日期:2022-10-31 接受日期:2022-12-26 出版日期:2023-10-15 发布日期:2023-10-27
  • 通讯作者: 李永平
  • 作者简介:吴兴富 E-mail:ynyxwxf@163.com
  • 基金资助:
    中国烟草总公司云南省公司科技计划项目(2017YN05)

Phenotypic Genetic Diversity Analysis of Leaf Shrinkage and Economic Traits of Flue-cured Tobacco for Germplasm Resources

Xingfu WU(), Fangchan JIAO, Xuejun CHEN, Zhiyu FANG, Guanghai ZHANG, Yihan ZHANG, Yongping LI()   

  1. Key Laboratory of Tobacco Biotechnological Breeding,Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650021,China
  • Received:2022-10-31 Accepted:2022-12-26 Online:2023-10-15 Published:2023-10-27
  • Contact: Yongping LI

摘要:

为了解烤烟种质资源叶片收缩性状和经济性状的遗传多样性,对158份烤烟资源的叶片收缩性状(中部8~10叶位)和经济性状进行遗传多样性分析、相关性分析、因子分析和聚类分析。结果表明,参试资源16个性状的变异系数为7.41%~73.36%,平均22.01%;遗传多样性指数为1.912 3~2.090 9,平均2.022 4。烤烟叶片收缩率表现为叶面积(33.21%)>叶宽(24.82%)>叶长(11.21%)。相关性分析表明,叶长、叶宽、叶面积的收缩值和收缩率与产量相关性不显著,与产值和均价呈极显著负相关;叶宽、叶面积的收缩值和收缩率与上等烟比例呈显著或极显著负相关。因子分析表明,叶片收缩因子与烟叶等级质量及产值因子具有负相关关系。158份参试资源在相似距离35.0处可聚为4个类群,类群间性状差异极显著,其中Ⅲ类群资源烘烤后叶片较大,收缩率小,经济性状好,聚在Ⅲ类群的6份资源‘云烟97’‘长脖黄’‘云烟116’‘峨山烤烟’‘红花云烟85’‘GL939’,其叶片收缩率中等至小,烟叶等级质量和种植效益突出。综上所述,158份烤烟资源在叶片收缩性状及经济性状上具有丰富的遗传多样性,叶片收缩过大对烟叶等级质量及产值有不利影响;6份优异资源可用于烟叶生产或优质品种选育。

关键词: 烤烟, 种质资源, 叶片收缩性状, 经济性状, 表型遗传多样性

Abstract:

To understand phenotypic genetic diversities of leaf shrinkage and economic traits,158 germplasm resources of flue-cured tobacco were as materials. The genetic diversity, correlation analysis, factor analysis and cluster analysis were carried out. The results showed that the coefficient of variation on 16 traits of the germplasms ranged from 7.41% to 73.36% with mean value 22.01%; the genetic diversity ranged from 1.912 3 to 2.090 9, with mean value 2.022 4. The shrinkage rate of flue-cured tobacco leaf was as follows: leaf area (33.21%)>leaf width (24.82%)>leaf length (11.21%). The correlation analysis showed that, similar to leaf length, no significant correlations were found between leaf width, leaf area shrinkage value, shrinkage rate and yield, however an extremely significant negative correlation was shown between output value and average price. As expected, leaf width, leaf area shrinkage value and shrinkage rate were significantly or extremely significantly negatively correlated with the proportion of high grade cured leaf. Factor analysis revealed that both leaves grade quality and output value factors were negatively correlated with leaf shrinkage factors. These 158 germplasms were clustered into 4 groups at a similar distance of 35.0 and there were extremely significant differences on the traits between the groups. Also, the leaves of group Ⅲ were larger after curing, the smaller leaf shrinkage rate, and the better economic traits. Leaf shrinkage rates of 6 excellent resources (‘Yunyan 97’‘Changbohuang’‘Yunyan 116’‘Eshankaoyan’‘Honghuayunyan 85’ and ‘GL939’)clustered in group Ⅲ were moderate to small, the outstanding grade quality and planting benefit. In conclusion, the 158 flue-cured tobacco germplasm resources showed high phenotypic genetic diversity of leaf shrinkage and economic traits. Excessive leaf shrinkage had adverse effects on flue-cured tobacco leaf grade quality and output value. Six excellent resources could be promising candidates for tobacco production or variety selection.

Key words: flue-cured tobacco, germplasm resources, leaf shrinkage traits, economic traits, phenotypic genetic diversity

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