小麦花粉孔发育相关TaINP1基因鉴定及表达分析

doi:10.13304/j.nykjdb.2024.0620

中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (4): 22-35.DOI: 10.13304/j.nykjdb.2024.0620

小麦花粉孔发育相关TaINP1基因鉴定及表达分析

马蓓¹^,²(), 公杰²(), 杜银柯²^,³, 甘雨薇¹^,², 程蓉²^,³, 朱波¹, 易丽霞¹(), 马锦绣²(), 高世庆²()

^1.长江大学农学院，湖北荆州 434025
^2.北京市农林科学院杂交小麦研究所，北京 100097
^3.新疆农业大学草业学院，乌鲁木齐 830052

收稿日期:2024-08-02 接受日期:2024-09-18 出版日期:2025-04-15 发布日期:2025-04-15
通讯作者: 易丽霞,马锦绣,高世庆
作者简介:马蓓 E-mail：18034656852@163.com
公杰E-mail：gjrice@163.com第一联系人：马蓓和公杰为共同第一作者。
基金资助:
北京市科技新星交叉项目(20220484204);北京市农林科学院创新能力建设专项(KJCX20230203);农业生物育种国家科技重大专项(2023ZD040230204);作物表型组学协同创新中心项目(KJCX20240406)

Identification and Expression Analysis of TaINP1 Gene Related to Pollen Pore Development in Wheat

Bei MA¹^,²(), Jie GONG²(), Yinke DU²^,³, Yuwei GAN¹^,², Rong CHENG²^,³, Bo ZHU¹, Lixia YI¹(), Jinxiu MA²(), Shiqing GAO²()

^1.College of Agriculture，Yangtze University，Hubei Jingzhou 434025，China
^2.Institute of Hybrid Wheat，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China
^3.College of Prataculture，Xinjiang Agricultural University，Urumqi 830052，China

Received:2024-08-02 Accepted:2024-09-18 Online:2025-04-15 Published:2025-04-15
Contact: Lixia YI,Jinxiu MA,Shiqing GAO

摘要/Abstract

摘要：

花粉孔径调控因子在植物萌发孔径形成中发挥着核心作用。为探究小麦花粉表面萌发的孔发育调控机理，深入探索与OsINP1同源的TaINP1基因家族在小麦萌发孔径形成中的调控功能，利用生物信息学策略，在小麦基因组中鉴定出53个小麦TaINP1基因，系统分析了其理化性质、启动子顺式作用元件、基因间的共线性关系。组织表达分析表明，TaINP2.2、TaINP2.5等基因在小麦穗部显著高表达； TaINP2.5、TaINP3.2等基因经低温（10 ℃）处理后在花粉中高表达。结合花粉萌发及纳米磁珠转化技术，将RFP报告基因成功导入小麦花粉中，并利用激光共聚焦显微镜观测不同低温处理花粉的荧光强度，证实TaINP1基因在调控花粉孔开闭中起重要作用。以上研究结果为小麦花粉孔发育的调控机理奠定了基础，也为优化小麦花粉纳米磁珠高效转化体系提供了参考，为小麦分子育种遗传改良开辟了新途径。

关键词: 小麦, 花粉孔发育, INP1基因家族, 表达模式, 低温

Abstract:

Pollen pore size regulators play central roles in the formation of plant germination pore size. To study the regulation of germination pore development on the pollen surface of wheat and explore the regulatory function of the TaINP1 gene family homologous to OsINP1 in the formation of germination pore size in wheat， 53 members of wheat TaINP1 gene family were identified in the wheat genome using bioinformatics strategies， and their physicochemical properties， promoter cis acting elements and collinearity relationship between genes were systematically analyzed. Tissue expression analysis showed that TaINP2.2 and TaINP2.5 genes were significantly expressed in panicle of wheat， some TaINP1 genes， such as TaINP2.5 and TaINP3.2 etc， were highly induced in pollen after low temperature （10 ℃） treatment. Combined with pollen germination and nano-magnetic bead conversion technology， the RFP reporter gene was successfully introduced into wheat pollen， and the fluorescence intensity of pollen at different low temperatures was observed by laser confocal microscopy， the results showed that the TaINP1 genes played important roles in regulating pollen pore opening and closing. Above results provided basis for the regulation mechanism of wheat pollen pore， and also provided reference for optimizing the efficient transformation system of wheat pollen nanobeads， and also opened up a new way for genetic improvement of wheat molecular breeding.

Key words: wheat, pollen development, INP1 gene family, expression patterns, conversion solution, low temperature

中图分类号:

S512.1

马蓓, 公杰, 杜银柯, 甘雨薇, 程蓉, 朱波, 易丽霞, 马锦绣, 高世庆. 小麦花粉孔发育相关TaINP1基因鉴定及表达分析[J]. 中国农业科技导报, 2025, 27(4): 22-35.

Bei MA, Jie GONG, Yinke DU, Yuwei GAN, Rong CHENG, Bo ZHU, Lixia YI, Jinxiu MA, Shiqing GAO. Identification and Expression Analysis of TaINP1 Gene Related to Pollen Pore Development in Wheat[J]. Journal of Agricultural Science and Technology, 2025, 27(4): 22-35.

图/表 11

参考文献 22

1	HUANG J B, YANG L, YANG L, et al.. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae [J].Nature, 2023,614(7947):303-308.
2	YU X B, ZHANG X C, ZHAO P, et al.. Fertilized egg cells secrete endopeptidases to avoid polytubey [J]. Nature, 2021,592:433-437.
3	ZHANG Z B, SUN M K, XIONG T, et al.. Development and genetic regulation of pollen intine in Arabidopsis and rice [J/OL].Gene, 2024,893:147936[2024-07-20]..
4	SHI J X, CUI M H, YANG L, et al.. Genetic and biochemical mechanisms of pollen wall development [J]. Trends Plant Sci., 2015,20(11):741-753.
5	LI F S, PHYO P, JACOBOWITZ J, et al.. The molecular structure of plant sporopollenin [J]. Nat. Plants, 2019,5(1):41-46.
6	QUILICHINI T D, GRIENENBERGER E, DOUGLAS C J. The biosynthesis,composition and assembly of the outer pollen wall:a tough case to crack [J]. Phytochemistry, 2015,113:170-182.
7	FURNESS C A, RUDALL P J. Pollen aperture evolution:a crucial factor for eudicot success? [J]. Trends Plant Sci., 2004,9(3):154-158.
8	EDLUND A F, SWANSON R, PREUSS D. Pollen and stigma structure and function:the role of diversity in pollination [J].Plant Cell, 2004,16(Sl):S84-S97.
9	ZHANG X, ZHAO G C, TAN Q, et al.. Rice pollen aperture formation is regulated by the interplay between OsINP1 and OsDAF1 [J]. Nat. Plants, 2020,6(4):394-403.
10	DOBRITSA A A, REEDER S H. Formation of pollen apertures in Arabidopsis requires an interplay between male meiosis,development of INP1-decorated plasma membrane domains,and the callose wall [J/OL]. Plant Signal. Behav., 2017,12(12):e1393136 [2024-07-20]. .
11	DOBRITSA A A, COERPER D. The novel plant protein INAPERTURATE POLLEN1 marks distinct cellular domains and controls formation of apertures in the Arabidopsis pollen exine [J]. Plant cell, 2012,24(11):4452-4464.
12	LI P, BEN-MENNI SCHULER S, REEDER S H, et al.. INP1 involvement in pollen aperture formation is evolutionarily conserved and may require species-specific partners [J]. J. Exp.Bot., 2018,69(5):983-996.
13	DOBRITSA A A, KIRKPATRICK A B, REEDER S H, et al.. Pollen aperture factor INP1 acts late in aperture formation by excluding specific membrane domains from exine deposition [J]. Plant Physiol., 2018,176(1):326-339.
14	DE SOUSA T, RIBEIRO M, SABENÇA C, et al.. The 10,000-year success story of wheat! [J/OL]. Foods, 2021,10(9):2124 [2024-07-20]. .
15	CARRILLO-BARRAL N, DEL CARMEN RODRÍGUEZ-GACIO M, MATILLA A J. Delay of germination-1 (DOG1):a key to understanding seed dormancy [J/OL]. Plants (Basel), 2020,9(4):480 [2024-07-20]. .
16	LESCOT M, DÉHAIS P, THIJS G, et al.. PlantCARE,a database of plant Cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences [J]. Nucleic Acids Res., 2002,30(1):325-327.
17	ZHANG J P, QI Y N, WANG L M, et al.. Genomic comparison and population diversity analysis provide insights into the domestication and improvement of flax [J/OL]. iScience, 2020,23(4):100967 [2024-07-20]. .
18	SONG S Q, TANG C F, JIANG X C, et al.. Research progress on DOG1, a key regulator of seed dormancy and germination [J]. Plant Sci., 2024, 42(2): 254-266.
19	崔荣秀,张议文,陈晓倩,等.植物bZIP参与胁迫应答调控的最新研究进展[J].生物技术通报,2019,35(2):143-155.
	CUI R X, ZHANG Y W, CHEN X Q, et al.. The latest research progress on the stress responses of bZIP involved in plants [J].Biotechnol. Bull., 2019,35(2):143-155.
20	杜朝金,张汉尧,罗心平,等.基因调控植物花器官发育的研究进展[J].植物遗传资源学报,2024,25(2):151-161.
	DU C J, ZHANG H R, LUO X P, et al.. Progress in gene regulation of plant floral organ development [J]. J. Plant Genetic Resour., 2024, 25(2):151-161
21	WANG Z P, ZHANG Z B, ZHENG D Y, et al.. Efficient and genotype independent maize transformation using pollen transfected by DNA-coated magnetic nanoparticles [J]. J. Integr. Plant Biol., 2022,64(6):1145-1156.
22	WILKINS M R, GASTEIGER E, BAIROCH A, et al.. Protein identification and analysis tools in the ExPASy server [J]. Methods Mol. Biol., 1999,112:531-552.

基因名称 Gene name	正向引物 Forward primer （5’-3’）	反向引物 Reverse primer （5’-3’）
TaINP2.2	TCGTAGATGGGATAATGGGGC	CAAGCCACAGGAAGCACCTT
TaINP2.5	ATTGGGTTTCAGACGGAGCA	CAAGGGCTTGTCAGACCTGT
TaINP2.8	CCGCTGGAATCTTGTCCGTTA	TTCCTTGCAGCCTCACGATT
TaINP3.2	TCCATGAGTGGAAATGGGGC	ACTACGCAGCTCAGTATCGC
TaINP3.7	TTTTAAAGCTTGAACCCCTAACTGA	ACCCAGCCAATGTTTCTGCC
TaINP3.12	TTTTAAAGCTTGAACCCCTAACTGA	CCGATAGACCCAGCCAACG
TaINP3.4	GCAGTTGATCCTCAGGCACT	AGGGTTTGTAGCCGCAGATT
TaINP3.9	CTCAGCACACCATCCCAACA	TCTTCCACATTCCCGACAGC
TaINP3.14	CAGGCTGATAATCTTCGTCTGC	GTGCAGATTGGCGAGTGGTT
TaINP4.1	CCAAAAGGTGGTATTGCGGA	ACTGCTGCTCTGTTAGAGGC
TaINP4.4	ACTCCAGAAAGCTCGACAGC	CAGAGAGAACTGAGGGCACG
18s	CGCGCGCTACGGCTTTGACCTA	CGGCAGATTCCCACGCGTTACG

基因名称 Gene name	正向引物 Forward primer （5’-3’）	反向引物 Reverse primer （5’-3’）
TaINP2.2	TCGTAGATGGGATAATGGGGC	CAAGCCACAGGAAGCACCTT
TaINP2.5	ATTGGGTTTCAGACGGAGCA	CAAGGGCTTGTCAGACCTGT
TaINP2.8	CCGCTGGAATCTTGTCCGTTA	TTCCTTGCAGCCTCACGATT
TaINP3.2	TCCATGAGTGGAAATGGGGC	ACTACGCAGCTCAGTATCGC
TaINP3.7	TTTTAAAGCTTGAACCCCTAACTGA	ACCCAGCCAATGTTTCTGCC
TaINP3.12	TTTTAAAGCTTGAACCCCTAACTGA	CCGATAGACCCAGCCAACG
TaINP3.4	GCAGTTGATCCTCAGGCACT	AGGGTTTGTAGCCGCAGATT
TaINP3.9	CTCAGCACACCATCCCAACA	TCTTCCACATTCCCGACAGC
TaINP3.14	CAGGCTGATAATCTTCGTCTGC	GTGCAGATTGGCGAGTGGTT
TaINP4.1	CCAAAAGGTGGTATTGCGGA	ACTGCTGCTCTGTTAGAGGC
TaINP4.4	ACTCCAGAAAGCTCGACAGC	CAGAGAGAACTGAGGGCACG
18s	CGCGCGCTACGGCTTTGACCTA	CGGCAGATTCCCACGCGTTACG

基因名称 Gene name	基因号 Gene ID	染色体Chromosome	蛋白长度Number of amino acid/aa	分子量Molecular weight/Da	等电点 pI	不稳定指数Instability index	脂肪系数Aliphatic index	总亲水性指数 GRAVY	亚细胞定位Subcellular location
TaINP1.4	TraesCS1B02G127400	1B	333	36 564.21	6.14	54.41	83.60	-0.416	细胞核Nucleus
TaINP6.2	TraesCS6A02G248300	6A	232	25 301.97	9.61	67.01	93.15	-0.088	细胞核Nucleus
TaINP3.6	TraesCS3B02G120900	3B	260	27 937.63	5.43	54.74	86.58	-0.181	细胞核Nucleus
TaINP2.4	TraesCS2A02G526300	2A	419	46 270.21	6.24	59.92	71.89	-0.536	细胞核Nucleus
TaINP3.3	TraesCS3A02G306200	3A	296	31 824.06	5.63	49.12	88.34	-0.210	细胞核Nucleus
TaINP3.11	TraesCS3D02G105800	3D	260	27 976.71	5.46	55.33	85.85	-0.202	细胞核Nucleus
TaINP1.9	TraesCS1D02G400300	1D	226	2 449.19	5.65	46.52	98.14	-0.078	线粒体Mitochondria
TaINP1.6	TraesCS1B02G420300	1B	226	24 449.82	5.65	47.19	96.86	-0.098	线粒体Mitochondria
TaINP5.3	TraesCS5B02G265300	5B	525	58 620.02	6.58	70.75	76.63	-0.560	细胞核Nucleus
TaINP4.2	TraesCS4A02G183400	4A	539	58 911.63	6.44	54.62	69.13	-0.568	细胞核Nucleus
TaINP5.2	TraesCS5A02G265600	5A	518	57 856.21	6.51	68.93	77.30	-0.550	细胞核Nucleus
TaINP1.1	TraesCS1A02G096300	1A	335	36 675.16	5.71	57.88	82.27	-0.438	细胞核Nucleus
TaINP1.8	TraesCS1D02G276100	1D	481	53 546.29	5.95	62.72	74.39	-0.503	细胞核Nucleus
TaINP4.1	TraesCS4A02G126300	4A	335	37 349.28	7.87	51.81	84.00	-0.491	细胞核Nucleus
TaINP7.2	TraesCS7B02G114300	7B	448	48 464.72	7.36	56.89	82.01	-0.285	细胞核Nucleus
TaINP1.5	TraesCS1B02G285800	1B	526	58 065.31	5.79	61.88	73.23	-0.471	细胞核Nucleus
TaINP6.4	TraesCS6B02G276400	6B	232	25 314.02	9.46	68.12	92.72	-0.100	叶绿体Chloroplast
TaINP4.5	TraesCS4D02G129900	4D	537	58 671.34	6.41	54.16	68.47	-0.567	细胞核Nucleus
TaINP3.13	TraesCS3D02G295800	3D	292	31 490.63	5.37	49.44	89.55	-0.191	叶绿体Chloroplast
TaINP3.9	TraesCS3B02G365100	3B	477	51 918.14	6.78	50.58	75.68	-0.494	细胞核Nucleus
TaINP4.3	TraesCS4B02G135000	4B	566	6 217.82	6.40	56.40	66.70	-0.614	细胞核Nucleus
TaINP2.9	TraesCS2D02G529000	2D	425	46 843.83	5.86	57.18	73.39	-0.507	细胞核Nucleus
TaINP5.1	TraesCS5A02G174200	5A	467	51 251.99	7.02	53.54	77.41	-0.473	细胞核Nucleus
TaINP2.6	TraesCS2B02G512700	2B	240	26 178.71	9.20	44.06	86.33	-0.215	细胞质Cytoplasm

基因名称 Gene name	基因号 Gene ID	染色体Chromosome	蛋白长度Number of amino acid/aa	分子量Molecular weight/Da	等电点 pI	不稳定指数Instability index	脂肪系数Aliphatic index	总亲水性指数 GRAVY	亚细胞定位Subcellular location
TaINP1.4	TraesCS1B02G127400	1B	333	36 564.21	6.14	54.41	83.60	-0.416	细胞核Nucleus
TaINP6.2	TraesCS6A02G248300	6A	232	25 301.97	9.61	67.01	93.15	-0.088	细胞核Nucleus
TaINP3.6	TraesCS3B02G120900	3B	260	27 937.63	5.43	54.74	86.58	-0.181	细胞核Nucleus
TaINP2.4	TraesCS2A02G526300	2A	419	46 270.21	6.24	59.92	71.89	-0.536	细胞核Nucleus
TaINP3.3	TraesCS3A02G306200	3A	296	31 824.06	5.63	49.12	88.34	-0.210	细胞核Nucleus
TaINP3.11	TraesCS3D02G105800	3D	260	27 976.71	5.46	55.33	85.85	-0.202	细胞核Nucleus
TaINP1.9	TraesCS1D02G400300	1D	226	2 449.19	5.65	46.52	98.14	-0.078	线粒体Mitochondria
TaINP1.6	TraesCS1B02G420300	1B	226	24 449.82	5.65	47.19	96.86	-0.098	线粒体Mitochondria
TaINP5.3	TraesCS5B02G265300	5B	525	58 620.02	6.58	70.75	76.63	-0.560	细胞核Nucleus
TaINP4.2	TraesCS4A02G183400	4A	539	58 911.63	6.44	54.62	69.13	-0.568	细胞核Nucleus
TaINP5.2	TraesCS5A02G265600	5A	518	57 856.21	6.51	68.93	77.30	-0.550	细胞核Nucleus
TaINP1.1	TraesCS1A02G096300	1A	335	36 675.16	5.71	57.88	82.27	-0.438	细胞核Nucleus
TaINP1.8	TraesCS1D02G276100	1D	481	53 546.29	5.95	62.72	74.39	-0.503	细胞核Nucleus
TaINP4.1	TraesCS4A02G126300	4A	335	37 349.28	7.87	51.81	84.00	-0.491	细胞核Nucleus
TaINP7.2	TraesCS7B02G114300	7B	448	48 464.72	7.36	56.89	82.01	-0.285	细胞核Nucleus
TaINP1.5	TraesCS1B02G285800	1B	526	58 065.31	5.79	61.88	73.23	-0.471	细胞核Nucleus
TaINP6.4	TraesCS6B02G276400	6B	232	25 314.02	9.46	68.12	92.72	-0.100	叶绿体Chloroplast
TaINP4.5	TraesCS4D02G129900	4D	537	58 671.34	6.41	54.16	68.47	-0.567	细胞核Nucleus
TaINP3.13	TraesCS3D02G295800	3D	292	31 490.63	5.37	49.44	89.55	-0.191	叶绿体Chloroplast
TaINP3.9	TraesCS3B02G365100	3B	477	51 918.14	6.78	50.58	75.68	-0.494	细胞核Nucleus
TaINP4.3	TraesCS4B02G135000	4B	566	6 217.82	6.40	56.40	66.70	-0.614	细胞核Nucleus
TaINP2.9	TraesCS2D02G529000	2D	425	46 843.83	5.86	57.18	73.39	-0.507	细胞核Nucleus
TaINP5.1	TraesCS5A02G174200	5A	467	51 251.99	7.02	53.54	77.41	-0.473	细胞核Nucleus
TaINP2.6	TraesCS2B02G512700	2B	240	26 178.71	9.20	44.06	86.33	-0.215	细胞质Cytoplasm

基因名称 Gene name	基因号 Gene ID	染色体Chromosome	蛋白长度Number of amino acid/aa	分子量Molecular weight/Da	等电点 pI	不稳定指数Instability index	脂肪系数Aliphatic index	总亲水性指数 GRAVY	亚细胞定位Subcellular location
TaINP3.4	TraesCS3A02G334100	3A	475	51 766.93	6.53	52.32	74.36	-0.514	细胞核Nucleus
TaINP4.4	TraesCS4B02G178600	4B	334	37 268.18	7.87	50.04	82.22	-0.513	细胞核Nucleus
TaINP4.5	TraesCS3B02G220400	3B	327	36 381.83	7.79	59.51	76.51	-0.622	细胞核Nucleus
TaINP3.5	TraesCS3A02G372400	3A	557	60 348.69	6.31	59.06	70.09	-0.472	细胞核Nucleus
TaINP6.6	TraesCS6D02G230400	6D	232	25 274.05	9.97	65.71	96.08	-0.029	叶绿体Chloroplast
TaINP1.3	TraesCS1A02G392200	1A	226	24 449.82	5.47	43.61	98.14	-0.073	线粒体Mitochondria
TaINP6.3	TraesCS6B02G193200	6B	458	50 063.37	7.30	52.50	76.05	-0.464	细胞核Nucleus
TaINP3.14	TraesCS3D02G327600	3D	477	51 979.22	6.53	53.94	75.47	-0.495	细胞核Nucleus
TaINP1.2	TraesCS1A02G276600	1A	481	53 549.34	6.29	63.78	73.97	-0.512	细胞核Nucleus
TaINP1.7	TraesCS1D02G105300	1D	338	37 042.89	6.56	58.57	84.97	-0.371	细胞核Nucleus
TaINP3.12	TraesCS3D02G194800	3D	327	36 386.85	8.49	59.18	76.51	-0.626	细胞核Nucleus
TaINP2.1	TraesCS2A02G010800	2A	198	21 663.49	9.56	54.16	83.54	-0.271	细胞核Nucleus
TaINP3.1	TraesCS3A02G103500	3A	260	27 872.59	5.60	50.35	85.85	-0.191	叶绿体Chloroplast
TaINP4.6	TraesCS4D02G180200	4D	329	36 682.46	7.87	52.80	81.40	-0.527	胞核Nucleus
TaINP5.5	TraesCS5D02G273500	5D	517	57 757.09	6.55	68.94	77.43	-0.544	细胞核Nucleus
TaINP2.8	TraesCS2D02G096800	2D	294	32 644.01	6.01	60.35	78.10	-0.431	细胞核Nucleus
TaINP3.2	TraesCS3A02G190700	3A	350	38 979.69	6.36	59.18	75.11	-0.647	细胞核Nucleus

小麦花粉孔发育相关TaINP1基因鉴定及表达分析

Identification and Expression Analysis of TaINP1 Gene Related to Pollen Pore Development in Wheat

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 22

相关文章 15

编辑推荐

Metrics

[1]	薛振宇, 张康康, 张元元, 闫强强, 姚立蓉, 张宏, 孟亚雄, 司二静, 李葆春, 马小乐, 王化俊, 汪军成. 优质抗旱小麦种质的筛选及功能基因检测[J]. 中国农业科技导报, 2025, 27(1): 35-49.
[2]	孙宪印, 牟秋焕, 米勇, 吕广德, 亓晓蕾, 孙盈盈, 尹逊栋, 王瑞霞, 吴科, 钱兆国, 赵岩, 高明刚. 基于GT双标图对小麦新品系的分类评价[J]. 中国农业科技导报, 2024, 26(7): 14-24.
[3]	鲍新跃, 陈红敏, 王伟伟, 唐益苗, 房兆峰, 马锦绣, 汪德州, 左静红, 姚占军. 小麦TaCOBL-5基因克隆及表达分析[J]. 中国农业科技导报, 2024, 26(6): 11-21.
[4]	陈明迪, 胡桂花, 张海文, 王旺田. 水稻RR基因家族生物信息学及表达模式分析[J]. 中国农业科技导报, 2024, 26(5): 20-29.
[5]	张绥林, 李洋, 李琰, 张赟齐, 齐建勋, 侯智霞. 核桃晚霜危害特性及影响机制研究进展[J]. 中国农业科技导报, 2024, 26(4): 18-26.
[6]	赵刚, 王淑英, 李尚中, 张建军, 党翼, 王磊, 李兴茂, 程万莉, 周刚, 倪胜利, 樊廷录. 黄土旱塬区近40年降水对冬小麦耗水和产量的影响[J]. 中国农业科技导报, 2024, 26(3): 164-173.
[7]	张宏, 李卫国, 张晓东, 卢必慧, 张琤琤, 李伟, 马廷淮. 基于HJ-1星和GF-1号影像融合特征提取冬小麦种植面积[J]. 中国农业科技导报, 2024, 26(2): 109-119.
[8]	张景云, 关峰, 石博, 万新建. 小麦根系分泌物对苦瓜幼苗生长及土壤生物学环境的影响[J]. 中国农业科技导报, 2024, 26(2): 181-190.
[9]	李双, 王爱英, 焦浈, 池青, 孙昊, 焦涛. 盐胁迫下不同抗性小麦幼苗生理生化特性及转录组分析[J]. 中国农业科技导报, 2024, 26(2): 20-32.
[10]	苗宇, 王婕, 赵尧尧, 张丽佳, 刘美君. 低温胁迫后紫花苜蓿叶片光合作用的恢复特性研究[J]. 中国农业科技导报, 2024, 26(2): 80-89.
[11]	刘海霞, 张寅辉, 庄蕾, 郭梦娇, 赵李, 吴美娟, 侯健, 李甜, 刘红霞, 张学勇, 郝晨阳. 基于关联分析挖掘小麦SDS沉降值相关候选基因及KASP标记开发[J]. 中国农业科技导报, 2024, 26(12): 18-29.
[12]	杨朝阳, 徐鹏, 苑铁键, 李晓琼, 彭冬根, 张振涛, 杨俊玲, 丁闯闯, 朱纪洲. 杂交构树低温干燥特性及品质研究[J]. 中国农业科技导报, 2024, 26(11): 157-170.
[13]	胡墨明, 孟琰珺, 苗淑媛, 胡西宁, 许晴, 马红珍, 王鹏飞, 刘国芹, 康国章. 小麦淀粉合成关键基因TaAGPL1功能标记的开发[J]. 中国农业科技导报, 2024, 26(11): 43-55.
[14]	田鹏, 宋洁, 李霞, 王裕智, 武棒棒. 不同粒色小麦籽粒中叶酸及衍生物含量分析[J]. 中国农业科技导报, 2024, 26(11): 56-65.
[15]	尼格拉·阿布都外力, 魏修振, 门婧婕, 陈凌娜, 陈永坤, 黄耿青. 薰衣草 JAZ和MYC2-like基因家族生物信息学及表达模式分析[J]. 中国农业科技导报, 2024, 26(11): 79-90.