薰衣草 JAZ和MYC2-like基因家族生物信息学及表达模式分析

doi:10.13304/j.nykjdb.2024.0423

摘要/Abstract

摘要：

JAZ蛋白和MYC2-like转录因子是茉莉酸（jasmonate，JA）类物质信号转导途径的关键调节因子，在植物的生长发育和次级代谢中发挥重要作用。以拟南芥13个JAZ蛋白和11个MYC2-like蛋白的氨基酸序列为参考序列，检索薰衣草基因组中的LaJAZ和LaMYC2-like基因家族成员，并对其进行生物信息学分析。结果表明，在薰衣草基因组中共鉴定到26个LaJAZ和26个LaMYC2-like基因，这些基因不均匀的分布在22条染色体上。LaJAZ和LaMYC2-like基因启动子的顺式作用元件涉及植物对光、茉莉酸、脱落酸、防御和胁迫反应的响应。qRT-PCR显示，选择的6个LaJAZ基因在深紫色萼片中表达较高，而LaMYC2-like基因在浅紫色萼片中表达较高。此外，有15个LaJAZ和LaMYC2-like基因受茉莉酸甲酯（methyl jasmonate，MeJA）诱导。以上研究结果为薰衣草LaJAZ和LaMYC2-like基因的功能分析及其对JA信号的响应机理研究奠定了基础。

关键词: 薰衣草, JAZ家族, MYC2-like家族, 生物信息学, 表达模式, MeJA

Abstract:

JAZ protein and MYC2-like transcription factor， which act as key regulators of the jasmonate （JA） signal transduction pathway， play important roles on plant growth and development and specialized metabolisms. Using the amino acid sequences of 13 JAZ proteins and 11 MYC2-like proteins from Arabidopsis thaliana as reference sequences， the LaJAZ and LaMYC2-like genes in the Lavandula angustifolia genome were retrieved and subjected to bioinformatics analysis. The results showed that a total of 26 LaJAZ and 26 LaMYC2-like genes were identified in whole genome of Lavandula angustifolia， which were unevenly distributed on 22 chromosomes. The promoter cis-acting elements of LaJAZ and LaMYC2-like genes involved plant responses to light， jasmonate， abscisic acid， defense and stress reactions. qRT-PCR showed that the selected 6 LaJAZ genes had higher express level in deep purple sepals， while the selected LaMYC2-like genes had higher express level in light purple sepals. In addition， 15 LaJAZ and LaMYC2-like genes were induced by methyl jasmonate （MeJA）. Above results laid the foundation for the functional analysis of LaJAZ and LaMYC2-like genes in Lavandula angustifolia and their response mechanism to JA signaling.

Key words: Lavandula angustifolia, JAZ family, MYC2-like family, bioinformatics, expression pattern, MeJA

中图分类号:

S682.39

尼格拉·阿布都外力, 魏修振, 门婧婕, 陈凌娜, 陈永坤, 黄耿青. 薰衣草 JAZ和MYC2-like基因家族生物信息学及表达模式分析[J]. 中国农业科技导报, 2024, 26(11): 79-90.

Nigela Abuduwaili, Xiuzhen WEI, Jingjie MEN, Lingna CHEN, Yongkun CHEN, Gengqing HUANG. Bioinformatics and Expression Pattern Analysis of JAZ and MYC2-like Gene Family in Lavandula angustifolia[J]. Journal of Agricultural Science and Technology, 2024, 26(11): 79-90.

图/表 10

表1 薰衣草JAZ 基因的基本信息

Table 1 Basic information of JAZ genes in Lavandula angustifolia

基因名称 Gene name	基因ID Gene ID	染色体 Chromosome	起始位置 Initial position	终止位置 Termination position	氨基酸数量 Amino acids/aa	分子量 MW/kD	等电点 pI
LaJAZ1	La01G01624	Chr1	14 191 132	14 192 638	334	35.531 14	9.73
LaJAZ2	La01G02122	Chr1	22 315 757	22 318 426	279	29.241 02	7.64
LaJAZ3	La01G02748	Chr1	29 933 237	29 935 361	233	25.500 76	9.58
LaJAZ4	La01G02775	Chr1	30 304 246	30 305 952	270	29.719 64	8.74
LaJAZ5	La02G01205	Chr2	27 444 500	27 446 515	252	26.659 79	9.34
LaJAZ6	La04G00443	Chr4	23 907 703	2 908 702	207	21.913 95	8.46
LaJAZ7	La10G01957	Chr10	37 391 837	37 394 501	255	27.540 92	9.12
LaJAZ8	La11G01369	Chr11	30 835 601	30 838 263	297	31.243 32	8.31
LaJAZ9	La12G01015	Chr12	20 257 088	20 259 717	275	29.305 02	9.43
LaJAZ10	La14G00862	Chr14	9 169 268	9 170 127	211	22.548 44	9.44
LaJAZ11	La15G00121	Chr15	866 936	870 632	345	37.302 14	8.46
LaJAZ12	La15G00153	Chr15	1 075 151	1 079 126	212	22.390 18	9.80
LaJAZ13	La15G00189	Chr15	1 330 626	1 333 013	283	30.562 52	9.45
LaJAZ14	La15G01076	Chr15	25 198 964	25 199 826	213	22.829 80	9.93
LaJAZ15	La16G00168	Chr16	1 435 513	1 440 135	156	17.603 23	10.38
LaJAZ16	La17G00791	Chr17	17 083 687	17 085 577	226	24.465 89	9.93
LaJAZ17	La18G00414	Chr18	4 272 331	4 275 830	330	34.990 72	9.87
LaJAZ18	La19G01387	Chr19	22 164 844	22 167 383	136	15.788 10	9.52
LaJAZ19	La20G01484	Chr20	16 672 832	16 673 901	214	22.483 34	9.08
LaJAZ20	La20G02795	Chr20	29 446 294	29 450 179	190	20.995 00	10.19
LaJAZ21	La21G00568	Chr21	6 010 126	6 014 598	413	45.401 64	8.20
LaJAZ22	La21G00790	Chr21	16 075 757	16 078 401	278	29.511 56	9.81
LaJAZ23	La24G00748	Chr24	6 890 348	6 890 710	121	13.522 38	9.31
LaJAZ24	La00G00334	scaffold171	144 787	145 649	213	22.829 80	9.93
LaJAZ25	La00G00563	scaffold214	58 478	62 950	413	45.391 54	8.27
LaJAZ26	La00G03185	scaffold362	51 131	53 153	244	25.608 65	10.10

表2 薰衣草MYC2-like基因的基本信息

Table 2 Basic information of MYC2-like genes in Lavandula angustifolia

基因名称 Gene name	基因ID Gene ID	染色体 Chromosome	起始位置 Initial position	终止位置 Termination position	氨基酸数 Amino acids/aa	分子量 MW/kD	等电点 pI
LaGL1	La03G00379	Chr3	2 443 649	2 447 074	537	59.435 64	4.74
LaGL2	La13G01461	Chr13	28 879 003	28 882 769	564	62.550 64	5.89
LaGL3	La22G02008	Chr22	19 300 412	19 303 804	615	68.438 72	6.34
LaGL4	La23G02048	Chr23	15 406 101	15 409 527	615	68.475 87	7.08
LaGL5	La00G04274	scaffold463	39 967	46 925	1 260	139.787 86	7.35
LaJAM1	La04G02776	Chr4	19 290 825	19 295 838	488	54.222 49	6.37
LaJAM2	La04G01048	Chr4	6 403 237	6 405 850	607	67.699 30	7.29
LaJAM3	La09G00555	Chr9	10 822 064	10 824 378	586	65.382 98	8.34
LaJAM4	La09G02346	Chr9	26 062 346	26 064 746	500	56.370 18	7.70

图1 LaJAZ和LaMYC2-like基因在薰衣草染色体上的分布

Fig. 1 Chromosomal distribution of the LaJAZ and LaMYC2-like genes in Lavandula angustifolia

表2 薰衣草MYC2-like基因的基本信息 ( 续表Continued)

Table 2 Basic information of MYC2-like genes in Lavandula angustifolia

基因名称 Gene name	基因ID Gene ID	染色体 Chromosome	起始位置 Initial position	终止位置 Termination position	氨基酸数 Amino acids/aa	分子量 MW/kD	等电点 pI
LaJAM5	La11G00149	Chr11	952 209	952 997	214	23 742.54	6.64
LaJAM6	La14G01835	Chr14	35 018 055	35 020 479	487	53 926.27	5.73
LaJAM7	La15G00077	Chr15	455 788	457 275	496	54 676.16	6.17
LaJAM8	La20G01069	Chr20	13 368 557	13 371 145	488	54 015.28	6.39
LaJAM9	La23G01124	Chr23	8 221 465	8 223 969	604	67 064.71	7.03
LaJAM10	La26G00764	Chr26	4 697 504	4 699 730	509	57 161.84	7.48
LaJAM11	La27G00171	Chr27	2 153 629	2 156 648	571	63 635.30	8.47
LaJAM12	La00G05647	scaffold88	71 229	74 222	604	67 036.69	7.03
LaJAM13	La00G04428	scaffold479	84 467	87 484	571	63 579.26	8.52
LaMYC1	La01G01976	Chr1	19 882 771	19 885 227	659	71 878.19	5.25
LaMYC2	La11G01192	Chr11	26 776 504	26 778 951	655	71 246.38	5.11
LaMYC3	La13G00765	Chr13	12 863 123	12 864 978	476	52 637.39	6.80
LaMYC4	La13G00767	Chr13	13 043 198	13 044 704	476	52 623.36	6.80
LaMYC5	La14G01082	Chr14	19 151 335	19 158 157	678	75 041.97	6.39
LaMYC6	La14G01770	Chr14	34 640 706	34 642 877	617	66 918.74	5.00
LaMYC7	La15G00833	Chr15	16 057 347	16 059 330	598	66 439.99	5.93
LaMYC8	La19G01463	Chr19	25 336 517	25 338 519	474	52 241.72	6.06

图 2 JAZ 和MYC2-like蛋白的进化树分析A：JAZ蛋白的系统发育树；B：MYC2-like蛋白的系统发育树； —薰衣草；—拟南芥；—水稻

Fig. 2 Phylogenetic tree analysis of JAZ and MYC2-like proteinA： Phylogenetic tree of JAZ proteins； B： Phylogenetic tree of MYC2-like proteins； —Lavandula angustifolia； —Arabidopsis thaliana； —Oryza sativa

图3 LaJAZ 和 LaMYC2-like 基因编码蛋白的保守基序和基因结构分析A： LaJAZ 蛋白的保守基序组成；B： LaJAZ基因的结构；C： LaMYC2-like 蛋白的保守基序组成；D： LaMYC2-like 基因的结构

Fig. 3 Conserved motif and gene structure analysis of proteins encoded by LaJAZ and LaMYC2-like genesA： Conserved motif composition of LaJAZ proteins； B： Structure of LaJAZ genes； C： Conserved motif composition of LaMYC2-like proteins； D： Structure of LaMYC2-like genes

图4 LaJAZ和LaMYC2-like基因 2 000 bp上游启动子中顺式作用元件的分析A： LaJAZ启动子中顺式作用元件的分布；B： LaMYC2-like 启动子中顺式作用元件的分布

Fig. 4 Analysis of cis-elements in the 2 000 bp upstream promoter of LaJAZ and LaMYC2-like genesA： Distribution of cis-acting elements in the LaJAZ promoters； B： Distribution of cis-acting elements in the LaMYC2-like promoters

图5 LaJAZ和 LaMYC2-like基因在不同组织中的表达分析注：Petal—开花期深紫色花瓣；Sepal1—花蕾期深紫色萼片；Sepal2—开花期深紫色萼片；Sepal3—凋萎期深紫色萼片；Stem—2月龄的茎；Leaf—2 月龄的叶片。

Fig. 5 Expression analysis of LaJAZ and LaMYC2-like genes in different tissuesNote：Petal—Deep purple petals at the flowering stage； Sepal1—Deep purple sepals at the flower bud stage； Sepal2—Deep purple sepals at the blooming stage； Sepal3—Deep purple sepals at fade stage； Stem—Stems at 2-month-old stage；Leaf—Leaves at 2-month-old stage.

图 6 LaJAZ和LaMYC2-like基因在薰衣草不同组织中的表达分析A：用于基因表达分析的不同薰衣草组织；B：不同薰衣草组织中 LaJAZ 和 LaMYC2-like 基因的qRT-PCR表达分析。1—深紫色花瓣；2—花蕾期深紫色萼片；3—盛花期深紫色萼片；4—凋落期深紫色萼片；5—浅紫色花瓣；6—花蕾期浅紫色萼片；7—盛花期浅紫色萼片；8—凋落期浅紫色萼片；9—2月龄的根；10—2月龄的茎；11—2月龄的叶；不同小写字母表示在P<0.05水平差异显著。

Fig. 6 Expression analysis of LaJAZ and LaMYC2-like genes in different tissues of lavenderA： Different lavender tissues used for gene expression analysis； B： qRT-PCR analysis of expression of the LaJAZ and LaMYC2-like genes in different lavender tissues. 1—Deep purple petals； 2—Deep purple sepals in bud stage； 3—Deep purple sepals at blooming stage； 4—Deep purple sepals at fade stage； 5—Light purple petals； 6—Light purple sepals at bud stage； 7—Light purple sepals at blooming stage； 8—Light purple sepals at fade stage； 9—2-month-old roots； 10—2-month-old stems； 11—2-month-old leaves； different small letters indicate significant differences at P<0.05 level.

图 7 MeJA 处理 2 月龄薰衣草叶片后LaJAZ 和 LaMYC2-like基因的相对表达量注：*、**、***和****分别表示与0 h间在P<0.05、P<0.01、P<0.001、P<0.000 1水平差异显著。

Fig. 7 Relative expression of LaJAZ and LaMYC2-like genes was observed after MeJA treatment of 2-month-old lavender leavesNote： *， **， *** and **** indicate significant differences at P<0.05， P<0.01， P<0.001 and P<0.000 1 levels， repectively.

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