盐碱胁迫下燕麦蛋白组及代谢组联合分析

doi:10.13304/j.nykjdb.2024.0118

中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (5): 61-71.DOI: 10.13304/j.nykjdb.2024.0118

盐碱胁迫下燕麦蛋白组及代谢组联合分析

凌磊¹^,²(), 蒋慧欣²(), 李铭婧², 殷亚杰¹^,², 陈乃钰¹^,², 赵晓菊¹^,²()

^1.大庆师范学院黑龙江省油田应用化学与技术重点实验室，黑龙江大庆 163000
^2.大庆师范学院生物工程学院，黑龙江大庆 163712

收稿日期:2024-02-20 接受日期:2024-05-21 出版日期:2025-05-15 发布日期:2025-05-20
通讯作者: 赵晓菊
作者简介:凌磊 E-mail：13644602460@163.com
蒋慧欣 E-mail：dqsfxyjhx@126.com第一联系人：凌磊和蒋慧欣为共同第一作者。
基金资助:
黑龙江省自然科学项目基金(LH2021C001);大学生创新创业训练项目(202310235A001);大庆市指导科技计划项目(zd-2023-12);黑龙江省省属高等学校基本科研业务费项目(2023-KYYWF-0026)

Proteinome and Metabolome Combined to Analyze the Response Mechanism of Oat to Saline-alkali Stress

Lei LING¹^,²(), Huixin JIANG²(), Mingjing LI², Yajie YIN¹^,², Naiyu CHEN¹^,², Xiaoju ZHAO¹^,²()

^1.Heilongjiang Provincial Key Laboratory of Oilfield Applied Chemistry and Technology，Daqing Normal University，Heilongjiang Daqing 163000，China
^2.School of Biological Engineering，Daqing Normal University，Heilongjiang Daqing 163712，China

Received:2024-02-20 Accepted:2024-05-21 Online:2025-05-15 Published:2025-05-20
Contact: Xiaoju ZHAO

摘要/Abstract

摘要：

盐碱胁迫是限制植物生长的主要非生物胁迫之一。在盐碱地种植燕麦，可以使燕麦茎吸收土壤中的盐，并储存在燕麦秸秆中，以修复盐碱地。为明确盐碱胁迫对燕麦蛋白组及代谢组的影响，对4周龄燕麦进行6、12、24、48 h盐碱胁迫，测定不同胁迫后燕麦的生理生化指标，并对其蛋白组和代谢组进行分析。结果表明，随着盐碱胁迫时间的延长，燕麦的丙二醛含量总体上升，脯氨酸含量及超氧化物歧化酶、过氧化物酶、过氧化氢酶活性总体呈上升趋势。蛋白组和代谢组分析共鉴定到7 603个差异蛋白，其中上调3 217个、下调4 386个；差异代谢物855个。它们主要参与糖代谢、氨基酸代谢、光合作用和苯丙烷类生物合成等途径，包括番茄红素β-环化酶、环氧化酶和玉米黄质等与光合作用相关的关键酶。综上，盐碱胁迫增强了燕麦糖类、核苷酸、氨基酸等代谢物的积累，叶片中核糖体、苯丙烷类生物合成、乙醛酸和二羧酸代谢、光合生物中的碳固定是燕麦响应盐碱胁迫的主要途径。以上研究结果为盐碱土壤的恢复以及生态系统的重建提供了有效信息，为燕麦抗逆遗传改良和栽培奠定了理论基础。

关键词: 燕麦, 蛋白质组, 代谢组, 联合分析, 盐碱胁迫

Abstract:

Salt-alkali stress is one of the main abiotic stresses limits plant growth. Planting oats in saline-alkali land allows the oat stems to absorb salt from the soil and store it in oat straw to repair the saline-alkali land. In order to clarify the effects of salt-alkali stress on oat proteome and metabolome， four-week-old oats were treated with 6， 12， 24 and 48 h salt-alkali stress， and the physiological and biochemical properties of oats after salt-alkali stress were measured， and its proteome and metabolome were analyzed. The results showed that， with the prolongation of salt-alkali stress time， malondialdehyde content of oat increased overall， the proline content and the activities of superoxide dismutase， peroxidase， catalase generally showed an upward trend. Proteomic and metabolomic sequencing identified a total of 7 603 differential proteins， including 3 217 up-regulated， 4 386 down-regulated and 855 differential metabolites. They were mainly involved in pathways such as sugar metabolism， amino acid metabolism， photosynthesis and phenylpropanoid biosynthesis. Among them， lycopeneβ-cyclase， cyclooxygenase and zeaxanthin were key enzymes related to photosynthesis. In summary， salt-alkali stress enhanced the accumulation of metabolites such as sugars， nucleosides and amino acids. Ribosomes， phenylpropanoid biosynthesis， glyoxylic acid and dicarboxylic acid metabolism， and carbon fixation in photosynthetic organisms of leaves were the main pathways responsed salt-alkali stress. Above resltus provided effective informations for the restoration of saline-alkali soil and the reconstruction of ecosystems， and laid a theoretical foundation for the genetic improvement and cultivation of oat stress resistance.

Key words: oat, proteomics, metabolomics, joint analysis, saline-alkali stress

中图分类号:

S512.6

凌磊, 蒋慧欣, 李铭婧, 殷亚杰, 陈乃钰, 赵晓菊. 盐碱胁迫下燕麦蛋白组及代谢组联合分析[J]. 中国农业科技导报, 2025, 27(5): 61-71.

Lei LING, Huixin JIANG, Mingjing LI, Yajie YIN, Naiyu CHEN, Xiaoju ZHAO. Proteinome and Metabolome Combined to Analyze the Response Mechanism of Oat to Saline-alkali Stress[J]. Journal of Agricultural Science and Technology, 2025, 27(5): 61-71.

图/表 6

图 1 燕麦盐碱胁迫后生化指标

Fig. 1 Biochemical indicators after salinity stress

图 2 盐碱胁迫后燕麦蛋白组测序结果A：样本相关性热图，_1~_3分别表示3次重复；B：PCA分析；C：差异蛋白Venn图；D：差异蛋白统计

Fig. 2 Oat proteome sequencing results after saline stressA： Heat map of sample correlation，_1~_3 indicate 3 repetitions； B： PCA analysis； C： Venn diagram of differential proteins； D： Statistics of differential proteins

图 3 差异蛋白KEGG富集分析

Fig. 3 Differential protein KEGG enrichment analysis

图 4 不同盐碱胁迫处理下燕麦代谢组分析A：相关性热图，_1~_6分别表示6次重复；B：PCA分析；C：差异代谢物Venn图；D：KEGG富集

Fig. 4 Metabolome analysis of oat under different saline stress treatmentsA： Heat map of correlation，_1~_6 indicate 6 repetitions； B： PCA analysis； C： Venn diagram of differential metabolites； D： KEGG enrichment

图5 差异蛋白和差异代谢物OPLS荷载图注：pq［1］表示主成分与代谢物（蛋白）的协方差；pq［2］表示主成分与代谢物（蛋白）的相关系数。

Fig. 5 Loplots of differential protein and differential metabolite OPLSNote：Pq［1］ indicates covariance between principal component and metabolite （protein）；pq［2］ indicates corretation coefficient between principal component and metabolite （protein）.

图6 蛋白、代谢共同富集前10通路

Fig. 6 Protein and metabolism are jointly enriched in the top ten pathways

参考文献 32

1	YANG Y, GUO Y. Unraveling salt stress signaling in plants [J]. J. Integr. Plant Biol., 2018, 60(9):796-804.
2	SETIA R, GOTTSCHALK P, SMITH P,et al..Soil salinity decreases global soil organic carbon stocks [J].Sci. Total Environ., 2013, 465:267-272.
3	GUTIERREZ-GONZALEZ J J, GARVIN D F.Subgenome-specific assembly of vitamin E biosynthesis genes and expression patterns during seed development provide insight into the evolution of oat genome [J]. Plant Biotechnol. J., 2016,14(11):2147-2157.
4	HAN L P, WANG W H, ENEJI A E, et al.. Phytoremediating coastal saline soils with oats:accumulation and distribution of sodium,potassium,and chloride ions in plant organs [J]. J. Clean. Prod., 2015, 90:73-81.
5	WANG X S, REN H L, WEI Z W, et al.. Effects of neutral salt and alkali on ion distributions in the roots,shoots,and leaves of two alfalfa cultivars with differing degrees of salt tolerance [J]. J. Integr. Agric., 2017, 16(8):1800-1807.
6	SHEN Q, FU L, QIU L, et al.. Time-course of ionic responses and proteomic analysis of a tibetan wild barley at early stage under salt stress [J]. Plant Growth Regulation, 2016, 81(1):1-11
7	LI H, ZHANG W, HAN M, et al.. H₂S enhanced the tolerance of Malus hupehensis to alkaline salt stress through the expression of genes related to sulfur-containing compounds and the cell wall in roots [J]. Int. J. Mol. Sci., 2022, 23(23):1484-1493 .
8	李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:1-324.
9	SLAMA I, M’RABET R, KSOURI R, et al.. Effects of salt treatment on growth, lipid membrane peroxidation, polyphenol content, and antioxidant activities in leaves of Sesuvium portulacastrum L [J]. Arid Land Res. Manage., 2017, 31(4):404-417.
10	郭巧生,吴友根,林尤奋,等.广藿香苗期生长及其抗氧化酶活性对盐胁迫的响应[J].中国中药杂志,2009,34(5):530-534.
	GUO Q S, WU Y G, LIN Y F,et al.. Effect of NaCl stress on growth and antioxidant systems of Pogostemon cablin [J]. China J. Chin. Mater. Med., 2009, 34(5):530-534.
11	KUMARI A,DAS P, PARIDA A K,et al.. Proteomics,metabolomics,and ionomics perspectives of salinity tolerance in halophytes [J/OL]. Front.Plant Sci., 2015,6:537 [2024-01-20]. .
12	WANG Y, FENG Y F, YAN M, et al.. Effect of saline-alkali stress on sugar metabolism of jujube fruit [J/OL]. Horticulturae, 2022, 8(6):474 [2024-01-20]. .
13	XU Z S, CHEN X J, LU X P,et al..Integrative analysis of transcriptome and metabolome reveal mechanism of tolerance to salt stress in oat (Avena sativa L.) [J]. Plant Physiol.Biochem., 2021,160:315-328.
14	JIA X M, ZHU Y F, HU Y,et al.. Integrated physiologic,proteomic,and metabolomic analyses of Malus halliana adaptation to saline-alkali stress [J/OL]. Hortic.Res., 2019,6:91 [2024-01-20]. .
15	GHABOOLI M, KABOOSI E. Alleviation of the adverse effects of drought stress using a desert adapted endophytic fungus and glucose in tomato [J/OL]. Rhizosphere, 2022,21:481-493.
16	CHEN H Y, LI X E, LI Z G. Sugar signaling and its role in plant response to environmental stress [J]. Biol Bull-US, 2022, 38(7): 80-92.
17	HEINEMANN B, HILDEBRANDT T M. The role of amino acid metabolism in signaling and metabolic adaptation to stress-induced energy deficiency in plants [J]. J.Exp.Bot., 2021,72(13):4634-4645.
18	SADAK M S, SEKARA A, AL-ASHKAR I,et al.. Exogenous aspartic acid alleviates salt stress-induced decline in growth by enhancing antioxidants and compatible solutes while reducing reactive oxygen species in wheat [J/OL]. Front.Plant Sci., 2022,13:987641 [2024-01-20]. .
19	GUO Z F, YANG N, ZHU C H, et al.. Exogenously applied poly-γ-glutamic acid alleviates salt stress in wheat seedlings by modulating ion balance and the antioxidant system [J]. Environ. Sci. Pollut. Res., 2017, 24(7):6592-6598.
20	KHAN M N, MOBIN M, ABBAS Z K, et al.. Nitric oxide-induced synthesis of hydrogen sulfide alleviates osmotic stress in wheat seedlings through sustaining antioxidant enzymes,osmolyte accumulation and cysteine homeostasis [J]. Nitric Oxide, 2017,68: 91-102.
21	MEHAK G, AKRAM N A, ASHRAF M, et al.. Methionine-induced regulation of growth,secondary metabolites and oxidative defense system in sunflower (Helianthus annuus L.) plants subjected to water deficit stress [J/OL]. PLoS One,2021,16(12):e0259585 [2024-01-20]. .
22	XU Y, IBRAHIM I M, WOSU C I, et al.. Potential of new isolates of Dunaliella salina for natural β-carotene production [J/OL]. Biology,2018,7(1):E14 [2024-01-20]. .
23	CROFT H, CHEN J M, LUO X, et al.. Leaf chlorophyll content as a proxy for leaf photosynthetic capacity [J]. Glob.Change Biol., 2017, 23(9):3513-3524.
24	MORENO J C, MARTINEZ-JAIME S, KOSMACZ M, et al.. A multi-OMICs approach sheds light on the higher yield phenotype and enhanced abiotic stress tolerance in tobacco lines expressing the carrot lycopene β-cyclase1 gene [J/OL]. Front.Plant Sci.,2021,12:624365 [2024-01-20]. .
25	AHMADI I F, RASHID Y M. The impacts of salt stress on growth factors and photosynthesis pigments in wheat (Triticum aestivum L.) [J]. EAJSE, 2017, 3(2):58-67.
26	ANSARI H H, SIDDIQUI A, WAJID D, et al.. Profiling of energy compartmentalization in photosystem II (PSⅡ),light harvesting complexes and specific energy fluxes of primed maize cultivar (P1429) under salt stress environment [J]. Plant Physiol. Biochem., 2022, 170:296-306.
27	TANG C N, XIE J M, LYU J, et al.. Alleviating damage of photosystem and oxidative stress from chilling stress with exogenous zeaxanthin in pepper (Capsicum annuum L.) seedlings [J]. Plant Physiol. Biochem., 2021,162:395-409.
28	GAN T, LIN Z, BAO L, et al.. Comparative proteomic analysis of tolerant and sensitive varieties reveals that phenylpropanoid biosynthesis contributes to salt tolerance in mulberry [J/OL]. Int. J. Mol. Sci., 2021,22(17):9402 [2024-01-20]. .
29	KIM D, JEON S J, YANDERS S, et al.. MYB3 plays an important role in lignin and anthocyanin biosynthesis under salt stress condition in Arabidopsis [J]. Plant Cell Rep., 2022,41(7):1549-1560.
30	MA D Y, SUN D X, WANG C Y, et al.. Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress [J]. Plant Physiol. Biochem., 2014, 80:60-66.
31	JAYARAMAN K, VENKAT RAMAN K, SEVANTHI A M, et al.. Stress-inducible expression of chalcone isomerase2 gene improves accumulation of flavonoids and imparts enhanced abiotic stress tolerance to rice [J/OL]. Environ.Exp.Bot., 2021,190:104582 [2024-01-20]. .
32	WANG X, DAI W W, LIU C, et al.. Evaluation of physiological coping strategies and quality substances in purple sweet potato under different salinity levels [J]. Genes,2022,13(8):1350-1362.

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盐碱胁迫下燕麦蛋白组及代谢组联合分析

Proteinome and Metabolome Combined to Analyze the Response Mechanism of Oat to Saline-alkali Stress

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