小麦盐胁迫响应的代谢组学分析

doi:10.13304/j.nykjdb.2022.0340

摘要/Abstract

摘要：

盐胁迫极大地限制了小麦的稳产、增收，解析小麦应答盐胁迫的代谢机制，有助于优化小麦育种和栽培措施，提高盐碱地小麦产量。运用超高效液相色谱-串联质谱（UPLC-MS/MS）技术分析小麦根和叶在NaCl处理24 h后代谢谱的变化，解析其盐胁迫响应机制。正常生长和盐胁迫处理24 h的根、叶比较共检测到759种差异积累代谢物。根和叶响应盐胁迫处理的过程中，分别有207和147个代谢物质发生变化，其中有56个相同的差异积累代谢物。氨基酸及其衍生物、黄酮类化合物、有机酸及其衍生物、核苷酸及其衍生物这几类代谢物在小麦根和叶响应盐胁迫差异积累代谢物中占比共约50%，除此之外，脂类物质在叶的差异积累代谢物中占比约20%。这些物质在叶中主要参与代谢途径、光合作用、碳固定作用、糖代谢等通路，在根中主要参与次生代谢、氨基酸合成等通路。多种代谢物质间相互关联，组成小麦盐胁迫应答的调控网络，可能通过缓解盐胁迫带来的渗透胁迫和氧化胁迫等，提高小麦对盐胁迫的适应性。

关键词: 小麦, 盐胁迫, 代谢组学, 调控网络, 超高效液相色谱-串联质谱

Abstract:

The stable yield and income of wheat （Triticum aestivum L.） were greatly limited by salt stress. Elucidating the metabolic mechanism of wheat under salt stress will help to further optimize breeding and cultivation， thereby increasing wheat yields in saline-alkali soils. In this study， ultra performance liquid chromatography-tandem mass spectrometer（UPLC-MS/MS） was used to analyze metabolic profiling of wheat roots and leaves treated with salt stress， and to analyze their response mechanism. A total of 759 metabolites differentially accumulated in roots and leaves were identified under normal growth and 24 h salt treatment conditions. In response to salt stress， 207 and 147 metabolites were changed in roots and leaves， respectively， among which 56 were the same differentially accumulated metabolites. Amino acids and their derivatives， flavonoids， organic acids and their derivatives， nucleotides and their derivatives accounted for about 50% of the total differentially accumulated metabolites of wheat roots and leaves in response to salt stress. In addition， lipids accounted for about 20% of the differentially accumulated metabolites of wheat leaves. These differentially accumulated metabolites were mainly involved in metabolic pathways， photosynthesis， carbon fixation and glucose metabolism etc in leaves， and secondary metabolism and amino acid synthesis etc in roots. They were interrelated and constitute a regulatory network of salt stress response， which may improve the adaptability to salt stress in wheat by alleviating osmotic and oxidative stress caused by salt stress.

Key words: wheat, salt stress, metabolomics, regulation network, UPLC-MS/MS

中图分类号:

S512

姜雪敏, 陈向前, 李红燕, 姜奇彦. 小麦盐胁迫响应的代谢组学分析[J]. 中国农业科技导报, 2023, 25(9): 43-56.

Xuemin JIANG, Xiangqian CHEN, Hongyan LI, Qiyan JIANG. Metabolomic Analysis of Wheat Response to Salt Stress[J]. Journal of Agricultural Science and Technology, 2023, 25(9): 43-56.

图/表 10

参考文献 38

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编号Number	物质类别Class	数量Quantity	百分比Percentage/%
1	有机酸及其衍生物Organic acids and its derivatives	75	9.88
2	黄酮Flavone	68	8.96
3	氨基酸衍生物Amino acid derivatives	65	8.56
4	核苷酸及其衍生物Nucleotide and its derivates	58	7.64
5	黄酮碳糖苷Flavone C-glycosides	52	6.85
6	脂质-甘油磷脂Lipids_glycerophospholipids	37	4.87
7	羟基肉桂酰衍生物Hydroxycinnamoyl derivatives	36	4.74
8	其他Others	34	4.48
9	黄酮醇Flavonol	30	3.95
10	氨基酸Amino acids	29	3.82
11	酚胺Phenolamides	26	3.43
12	奎宁酸及其衍生物Quinate and its derivatives	22	2.90
13	植物激素Phytohormones	21	2.77
14	黄烷酮Flavanone	21	2.77
15	脂质-脂肪酸Lipids-fatty acids	20	2.64
16	脂质-甘油酯Lipids-glycerolipids	18	2.37

编号Number	物质类别Class	数量Quantity	百分比Percentage/%
1	有机酸及其衍生物Organic acids and its derivatives	75	9.88
2	黄酮Flavone	68	8.96
3	氨基酸衍生物Amino acid derivatives	65	8.56
4	核苷酸及其衍生物Nucleotide and its derivates	58	7.64
5	黄酮碳糖苷Flavone C-glycosides	52	6.85
6	脂质-甘油磷脂Lipids_glycerophospholipids	37	4.87
7	羟基肉桂酰衍生物Hydroxycinnamoyl derivatives	36	4.74
8	其他Others	34	4.48
9	黄酮醇Flavonol	30	3.95
10	氨基酸Amino acids	29	3.82
11	酚胺Phenolamides	26	3.43
12	奎宁酸及其衍生物Quinate and its derivatives	22	2.90
13	植物激素Phytohormones	21	2.77
14	黄烷酮Flavanone	21	2.77
15	脂质-脂肪酸Lipids-fatty acids	20	2.64
16	脂质-甘油酯Lipids-glycerolipids	18	2.37

编号Number	物质类别Class	数量Number	百分比Percentage/%
17	香豆素及其衍生物Coumarins and its derivatives	18	2.37
18	维生素类Vitamins	16	2.11
19	苯甲酸衍生物Benzoic acid derivatives	15	1.98
20	糖类Carbohydrates	14	1.84
21	色胺及其衍生物Tryptamine and its derivatives	11	1.45
22	黄酮-木脂素Flavonolignan	11	1.45
23	花青素Anthocyanins	10	1.32
24	吲哚及其衍生物Indole and its derivatives	8	1.05
25	生物碱Alkaloids	8	1.05
26	儿茶素及其衍生物Catechin and its derivatives	6	0.79
27	胆碱类Cholines	6	0.79
28	异黄酮Isoflavone	5	0.66
29	烟酸及其衍生物Nicotinic acid and its derivatives	5	0.66
30	萜类Terpenoids	5	0.66
31	醇类和多元醇Alcohols and polyols	5	0.66
32	吡啶Pyridine derivatives	3	0.40
33	原花青素Proanthocyanidins	1	0.13

编号Number	物质类别Class	数量Number	百分比Percentage/%
17	香豆素及其衍生物Coumarins and its derivatives	18	2.37
18	维生素类Vitamins	16	2.11
19	苯甲酸衍生物Benzoic acid derivatives	15	1.98
20	糖类Carbohydrates	14	1.84
21	色胺及其衍生物Tryptamine and its derivatives	11	1.45
22	黄酮-木脂素Flavonolignan	11	1.45
23	花青素Anthocyanins	10	1.32
24	吲哚及其衍生物Indole and its derivatives	8	1.05
25	生物碱Alkaloids	8	1.05
26	儿茶素及其衍生物Catechin and its derivatives	6	0.79
27	胆碱类Cholines	6	0.79
28	异黄酮Isoflavone	5	0.66
29	烟酸及其衍生物Nicotinic acid and its derivatives	5	0.66
30	萜类Terpenoids	5	0.66
31	醇类和多元醇Alcohols and polyols	5	0.66
32	吡啶Pyridine derivatives	3	0.40
33	原花青素Proanthocyanidins	1	0.13

编号Index	物质类别Class	物质Compound	Log₂（fold change）
编号Index	物质类别Class	物质Compound	CKL/S24L	CKR/CKL	CKR/S24R	S24R/S24L
Tri941	其他Others	3，4-二羟基杏仁酸Dl-3，4-dihydroxymandelic acid	13.075		11.951
Tri891	其他Others	N-乙酰葡萄糖胺1-磷酸N-acetylglucosamine 1-phosphate	12.500		13.449
Tri1073	黄酮Flavone	木犀草素O-己糖基-O-葡萄糖酸 Luteolin O-hexosyl-O-gluconic acid	10.722
Tri835	氨基酸Amino acids	L-半胱氨酸L-cysteine	10.131
Tri882	核苷酸及其衍生物Nucleotide and its derivates	腺苷5’-单磷酸Adenosine 5’-monophosphate	8.822	-16.655
Tri1166	黄酮Flavone	金圣草黄素 O-乙酰基己糖苷Chrysoeriol O-acetylhexoside	8.822
Tri1231	醇类和多元醇Alcohols and polyols	肠二醇Enterodiol	8.699
Tri1356	脂质-脂肪酸Lipids_fatty acids	13-羟基十八碳三烯酸13-HOTrE	7.756
Tri74	核苷酸及其衍生物Nucleotide and its derivates	异戊烯基-7-葡糖苷iP7G	7.635