Journal of Agricultural Science and Technology ›› 2025, Vol. 27 ›› Issue (1): 72-79.DOI: 10.13304/j.nykjdb.2023.0266

• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles     Next Articles

Physiological Regulation of Growth of Cotton Seedlings Under Salt Stress by Rhamnolipids

Xuewen XU1,2(), Xingpeng WANG1,2,3,4, Hongbo WANG1,2,3, Zhenxi CAO1,2,3()   

  1. 1.College of Water Hydraulic and Architectural Engineering,Tarim University,Xinjiang Alar 843300,China
    2.Modern Agricultural Engineering Key Laboratory at Universities of Education Department of Xinjiang Uygur Autonomous Region,Tarim University,Xinjiang Alar 843300,China
    3.Institute of Western Agriculture,Chinese Academy of Agricultural Sciences,Xinjiang Changji 831100,China
    4.Key Laboratory of Northwest Oasis Water-Saving Agriculture of Ministry of Agriculture and Rural Affairs,Xinjiang Academy of Agricultural and Reclamation Sciences,Xinjiang Shihezi 832000,China
  • Received:2023-04-06 Accepted:2023-06-19 Online:2025-01-15 Published:2025-01-21
  • Contact: Zhenxi CAO

鼠李糖脂对盐胁迫下棉花幼苗根系生长的调控作用

徐雪雯1,2(), 王兴鹏1,2,3,4, 王洪博1,2,3, 曹振玺1,2,3()   

  1. 1.塔里木大学水利与建筑工程学院,新疆 阿拉尔 843300
    2.塔里木大学,新疆维吾尔自治区教育厅现代农业工程重点实验室,新疆 阿拉尔 843300
    3.中国农业科学院西部农业研究所,新疆 昌吉 831100
    4.新疆农垦科学院,农业农村部西北绿洲节水农业重点实验室,新疆 石河子 832000
  • 通讯作者: 曹振玺
  • 作者简介:徐雪雯 E-mail: 1726477971@qq.com
  • 基金资助:
    塔里木大学校长基金项目(TDZKSS202248);国家重点研发计划项目(2022YFD1900505);新疆维吾尔自治区普通高等学校现代农业工程重点实验室开放课题项目(TDNG2023105)

Abstract:

To investigate the mitigating effect of rhamnolipids (RLS) on the physiological growth of cotton seedlings under salt stress, 2 salt gradients including low salt (3 g·L-1 NaCl, T1) and high salt (6 g·L-1 NaCl, T2) and 4 RLS gradients including 0(R0), 200(R1), 300(R2) and 400 mg·L-1 (R3)were set up to analysis the effects of RLS on the root morphology, antioxidant enzymes and osmoregulatory substances of cotton seedlings under salt stress. The results showed that the appropriate level of RLS under salt stress could promote the growth of total root length, root surface area and root tip number of cotton seedlings. Under low salt stress, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and contents of proline (Pro), soluble sugars (SS) in the roots of cotton seedlings firstly increased and then decreased with the increase of RLS content,with T1R2 showing an increase of 14.0%, 15.2%, 28.8%, 28.5% and 19.5%, respectively, compared with T1R0. The activities of SOD, POD, CAT and contents of Pro,SS in the roots of cotton seedlings under high salt stress showed a gradual decrease under high salt stress, with T2R1 treatment increasing 10.5%, 12.6%, 13.9%, 21.7% and 4.8%, respectively, compared with T2R0. Principal component analysis showed that the response of cotton seedling root surface area, SOD and Pro to the regulation of RLS under salt stress was high, and could be used as the main control indicators for the physiological growth regulation of cotton seedlings by RLS under salt stress. The optimum content of RLS applied under low salt stress to enhance the salt tolerance of cotton seedlings was 300 mg·L-1, while the optimum content of RLS applied under high salt stress was 200 mg·L-1. Above results provided a more suitable exogenous application strategy for cotton salt tolerance and stress tolerance planting.

Key words: salt stress, rhamnolipids(RLS), root morphology, physiological and biochemical properties, comprehensive evaluation

摘要:

为探究鼠李糖脂(rhamnolipids,RLS)对盐胁迫下棉花幼苗生理生长的缓解作用,设置低盐(3 g·L-1 NaCl,T1)和高盐(6 g·L-1 NaCl, T2)2个盐梯度,0(R0)、200(R1)、300(R2)和400 mg·L-1(R3)4个RLS梯度,分析盐胁迫下施加RLS对棉花幼苗根系形态、抗氧化酶及渗透调节物质的影响。结果表明,盐胁迫下适宜水平的RLS可促进棉花幼苗根系总长度、表面积和根尖数的增长。在低盐胁迫下,棉花幼苗根系超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)活性和脯氨酸(proline,Pro)、可溶性糖(soluble sugars,SS)含量随RLS含量升高呈先增大后减小的趋势,其中T1R2处理各项指标较T1R0分别提高14.0%、15.2%、28.8%、28.5%和19.5%;在高盐胁迫下,棉花幼苗根系SOD、POD、CAT活性和Pro、SS含量随RLS含量升高呈逐渐减小的趋势,其中T2R1较T2R0处理分别提高10.5%、12.6%、13.9%、21.7%和4.8%。主成分分析表明,盐胁迫下棉花幼苗根系表面积、SOD和Pro对RLS的调控响应度较高,可作为盐胁迫下RLS对棉花幼苗生理生长调控的主要控制指标。综合评价得出,低盐胁迫下施用RLS提升棉花幼苗耐盐性的最适宜量为300 mg·L-1,而高盐胁迫下RLS的最佳施用量为200 mg·L-1。以上研究结果为棉花耐盐抗逆种植提供了适宜的外源施用策略。

关键词: 盐胁迫, 鼠李糖脂, 根系形态, 生理特性, 综合评价

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