Studies on Drought Tolerance of IPT Transgenic Rice

doi:10.3969/j.issn.10080864.2012.06.

›› 2012, Vol. 14 ›› Issue (6): 30-35.DOI: 10.3969/j.issn.10080864.2012.06.

Studies on Drought Tolerance of IPT Transgenic Rice

MAIMAITI Yushanjiang1, XIONG Yehui1, MIJITI Maihemutijiang1, SEHROON Khan1,
ANDROW Cal2, ZENG Hongmei1, QIU Dewen1

1.Key Laboratory for Biological Control of Agricultural, Institute of Plant Protection, Chinese Academy of Agricultural
Sciences, Beijing 100081, China|2.Gene Research Lab. of Plant Breeding and Biochemistry Division, International Rice
Research Institute, Metro Manila, DAPO Box 7777, Philippines

Received:2012-05-22 Revised:2012-06-11 Online:2012-12-15 Published:2012-11-15

转IPT基因水稻的抗旱性研究

玉山江·麦麦提1,熊叶辉1,麦合木提江·米吉提1,Sehroon khan1,
Androw Cal2,曾洪梅1,邱德文1*

1.中国农业科学院植物保护研究所蛋白质农药研究室, 北京 100081|
2.国际水稻研究所作物育种和生物化学系转基因研究室, 马尼拉 DAPO Box 7777, 菲律宾

通讯作者: 邱德文，研究员，博士生导师，主要从事蛋白质药物研究。 E-mail: qiudewen@cass.net.cn
作者简介:玉山江·麦麦提,博士研究生,研究方向为药物工程。E-mail:yusanjan0418@yahoo.com.cn。通讯作者：邱德文|研究员|博士生导师|主要从事蛋白质药物研究。 E-mail: qiudewen@cass.net.cn
基金资助:
转基因生物新品种培育重大专项（2009ZX08001-018B）资助。

Abstract

Abstract:

Drought resistance has become a hot issue in rice scientific research. By Agrobacterium tumefaciens\|mediated, IPT gene was transformed to rice IR64 variety. 254 regenerated were obtained, and PCR and southern blotting analyze were carried on them to confirm integration of aim gene in the transgenic plant. Two transgenic single copy events and one wild type were treated with drought stress at flowering stage. Under the same drought stress condition, the cytokinins and chlorophyll contents of transgenic plants were obviously higher than that of the wild type. While the leaf temperature was slightly lower than that of the wild type. The stem and leaf senescence of transgenic plant was delayed. These result shows that IPT gene has positive significance on studying drought tolerance breeding and its mechanism.

Key words: rice, IPT, cytokinin, drought tolerance

摘要：

水稻抗旱性研究是当前水稻研究的热点。采用农杆菌介导法将IPT基因导入籼稻IR64，并获得了254株再生植株，并对其进行PCR检测和Southern杂交分析，确定目的基因已整合到水稻中。选择两个单拷贝的转基因株系和一个野生型株系在开花期对其进行干旱胁迫，在同等水平干旱胁迫条件下，IPT转基因植株的细胞分裂素含量和叶绿素含量明显高于野生型，叶片温度稍微低于野生型，且转基因植株茎叶衰老延缓，这些结果表明IPT基因在抗旱育种及机理研究方面有积极的研究意义。

关键词: 水稻;IPT;细胞分裂素;抗旱性

CLC Number:

S511
S188

MAIMAITI Yushanjiang1, XIONG Yehui1, MIJITI Maihemutijiang1, SEHROON Khan. Studies on Drought Tolerance of IPT Transgenic Rice[J]. , 2012, 14(6): 30-35.

玉山江·麦麦提1,熊叶辉1,麦合木提江·米吉提1,Sehroon khan1,. 转IPT基因水稻的抗旱性研究[J]. , 2012, 14(6): 30-35.

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Studies on Drought Tolerance of IPT Transgenic Rice

转IPT基因水稻的抗旱性研究

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