Effects of Zinc Fertilizer Application on Physiological Characteristics and Grain Zn Content of Maize in Saline-alkali Soil

doi:10.13304/j.nykjdb.2021.0385

Abstract

Abstract:

In order to study the effects of different zinc application rates on the physiological characteristics and grain zinc content of maize in saline-alkali land， a field experiment was conducted with Kehe 699 as the test material by random block design. 6 treatments were set up： T0（0 kg·hm^-2）， T1（7.5 kg·hm^-2）， T2（15 kg·hm^-2）， T3（22.5 kg·hm^-2）， T4（30 kg·hm^-2）， T5（37.5 kg·hm^-2）， and the physiological characteristics， grain zinc content and yield of maize under different treatments were measured. The results showed that zinc application decreased the relative conductivity， proline and MDA content of maize leaves in saline-alkali soil. Compared with T0 treatment， the relative conductivity， proline content and malondialdehyde content under T3 treatment were decreased by 22.3%， 24.7% and 15.9%， respectively in the filling stage. Zinc application increased the protective enzyme activity of maize leaves， and the superoxide dismutase（SOD）， peroxidase（POD） and catalase（CAT） activities in T3 treatment at big trumpet stage were increased by 68.7%， 152.1 % and 72.6% respectively， compared with in T0 treatment. At the same time， the zinc content in maize grain first increased and then decreased with the increase of zinc application rate， and reached the maximum value under T3 treatment. T3 treatment could improve the transfer rate and contribution rate of zinc in leaves and stems， reduce the bare tip of maize， increase ear length， grain number and 100-grain weight， thus increasing the zinc content and yield of maize grains. The results of this study showed that appropriate application of zinc fertilizer could improve the physiological activity of maize leaves and enhance the resistance of maize in saline-alkali environment， and the maize yield and grain zinc content were the highest when the zinc application rate was 22.5 kg·hm^-2.

Key words: maize, zinc fertilizer, physiological characteristics, grain zinc content

摘要：

为研究不同施锌水平对盐碱地玉米生理特性及籽粒锌含量的影响，以科河699为供试材料，采用随机区组设计进行田间试验，根据不同施锌肥方式设置6个处理：T0（0 kg·hm^-2）、T1（7.5 kg·hm^-2）、T2（15 kg·hm^-2）、T3（22.5 kg·hm^-2）、T4（30 kg·hm^-2）、T5（37.5 kg·hm^-2），测定不同处理下玉米生理特性、籽粒锌含量及产量。结果表明，施锌降低了盐碱地玉米叶片相对电导率、脯氨酸含量、丙二醛含量，灌浆期T3处理的叶片相对电导率、脯氨酸含量、丙二醛含量分别较T0处理降低了22.3%、24.7%、15.9%；施锌提高了玉米叶片保护性酶活性，大喇叭口期T3处理的超化物歧化酶（superoxide dismutase，SOD）、过氧化物酶（peroxidase，POD）和过氧化氢酶（catalase，CAT）活性分别较T0处理提高了68.7%、152.1%和72.6%；同时，玉米籽粒锌含量和产量随施锌量的增加呈先增加后降低的趋势，均在T3处理下达到最大值；T3处理能提高玉米叶片及茎秆中锌素的转移率及贡献率，减少玉米秃尖，增加穗长、穗粒数和百粒重，从而提高玉米籽粒锌含量和产量。以上结果表明，适量施锌肥可以提高玉米叶片生理活性，增强玉米对盐碱环境的抵抗能力，且当施锌量为22.5 kg·hm^-2时玉米产量及籽粒锌含量均最高。

关键词: 玉米, 锌肥, 生理特性, 籽粒锌含量

CLC Number:

S318

Qian YANG, Na WU, Cong ZHAO, Yu HAN, Zhonghua MA, Yongsen YANG, Jili LIU. Effects of Zinc Fertilizer Application on Physiological Characteristics and Grain Zn Content of Maize in Saline-alkali Soil[J]. Journal of Agricultural Science and Technology, 2022, 24(9): 166-176.

杨茜, 吴娜, 赵匆, 韩羽, 麻仲花, 杨永森, 刘吉利. 施锌对盐碱地玉米生理特性及籽粒锌含量的影响[J]. 中国农业科技导报, 2022, 24(9): 166-176.

Figures/Tables 10

References 33

1	沈汀兰.我国玉米产业发展与粮食安全[J].农经,2021(5): 44-49.
2	李新,许志斌,佘奎军,等.宁夏玉米产业的现状和发展[J].种子,2009,28(9):104-106.
	LI X, XU Z B, SHE K J, et al.. Status and development of maize industry in Ningxia province [J]. Seed, 2009, 28(9): 104-106.
3	李成军,吴宏亮,康建宏,等.宁夏中部干旱区玉米种植保护性耕作及覆盖措施研究[J].农业科学研究,2009,30(4):6-10.
	LI C J, WU H L, KANG J H, et al.. Study on protective tilling and plastic mulching technique to maize planting in the drought zone of middle Ningxia [J]. J.Agric. Sci., 2009,30(4): 6-10.
4	黄建成,陈国栋,李鹏.宁夏引黄灌区土壤盐渍化现状与改良[J].水土保持研究,2008,15(6):256-258.
	HANG J C, CHEN G D, LI P. Present situation and improvement of soil Salinity in directly yellow-irrigated areas of Ningxia [J]. Res. Soil Water Convers., 2008, 15(6): 256-258.
5	刘学军,刘平,蒋正文.宁夏扬黄灌区土壤盐渍化防治对策[J].宁夏农林科技,2018,59(9): 54-58.
	LIU X J, LIU P, JIANG Z W. Control of soil salinization in irrigation area of yellow river in Ningxia [J]. J. Ningxia For. Agric. Sci. Technol., 2018, 59(9):54-58.
6	孙文涛,汪仁,安景文,等.平衡施肥技术对玉米产量影响的研究[J].玉米科学,2008(3):109-111.
	SUN W T, WANG R, AN J W, et al.. Study on effect of balanced fertilization technology on the yield of corn [J].J. Maize Sci., 2008, 16(3):109-111.
7	孙建华.玉米施锌吸收积累及有效化调控机理的研究[D].长春:吉林农业大学,2013.
	SUN J H. Studies on influence of zinc fertilizer on the absorption and accumulation of zinc content and the regulatory mechanism of zinc availability [D].Changchun: Jilin Agricultual University, 2013.
8	张金尧,汪洪.锌肥施用与人体锌素营养健康[J].肥料与健康,2020,47(1):11-16, 36.
	ZHANG J Y, WANG H. Zinc fertilizer application and human Zinc nutrition health [J]. Fert. Health, 2020, 47(1): 11-16, 36.
9	李淑云,董小坤.论锌对人体健康的重要性[J].胜利油田师范专科学校学报,2000(4):52-53.
10	ISMAIL C. Tansley review No. 111: possible roles of zinc in protecting plant cells from damage by reactive oxygen species [J]. New Phytol., 2000, 146(2): 185-205.
11	LAMBERS H, BRUNDRETT M C, RAVEN J A, et al.. Plant mineral nutrition in ancient landscapes: high plant species diversity on infertile soils is linked to functional diversity for nutritional strategies [J]. Plant Soil, 2011, 348(1-2):7-27.
12	李文宗,张兰,徐妙云,等.富锌玉米的筛选及叶面喷施锌肥对玉米籽粒中矿物元素的影响分析[J]. 中国农业科技导报, 2018,20(1):47-54.
	LI W Z, ZHANG L, XU M Y, et al.. Screening of zinc rich corn and the investigation of mineral element in corn grain sprayed with zinc fertilizer [J]. J. Agric. Sci. Tech., 2018, 20(1):47-54.
13	ALLOWAY B J. Soil factors associated with zinc deficiency in crops and humans[J]. Environ. Geochem. Health, 2009, 31(5):537-548.
14	王子腾,耿元波.国内外主要粮食作物对施用锌肥响应的研究进展[J].植物营养与肥料学报,2018,24(3):805-816.
	WANG Z T, GENG Y B. Research advances on the response of main food crops to zinc fertilization at China and abroad [J]. J. Plant Nutr. Fert., 2018, 24(3): 805-816.
15	SHIVAY Y S, KUMAR D, PRASAD R, et al.. Relative yield and zinc uptake by rice from zinc sulphate and zinc oxide coatings onto urea [J]. Nutr. Cycl. Agroecosyst.,2008,80(2): 181-188.
16	尚清芳,董莉丽,张建明,等.宁夏银北平原灌淤土耕层土壤微量元素的空间变异性研究[J].安徽农业科学,2010,38(32): 18173-18176.
	SHANG Q F, DONG L F, ZHANG J M, et al.. Spatial variability of trace elements in irrigation-silted soil in north of yinchuan plain of Ningxia [J]. J. Anhui Agric. Sci., 2010, 38(32): 18173-18176.
17	曹婷,代惠萍.锌胁迫对4种紫花苜蓿叶片生理特性的影响[J].湖北农业科学,2014(10):2365-2367.
	CAO T, DAI H P. Biochemical characteristics of different alfalfa leaves under zinc stress [J]. Hubei Agric. Sci., 2014(10): 2365-2367.
18	王兴义.锌、铁肥种子处理对玉米生长发育及光合特性的影响[D].杨凌:西北农林科技大学,2016.
	WANG X Y. Effect of seed treatment of zinc and iron fertilizer on the growth and photosynthetic characteristics of maize [D]. Yangling: Northwest A & F University, 2016.
19	杨建肖,杨国航,孙世贤,等.硫酸锌处理对玉米种子萌发的生理效应[J].植物营养与肥料学报,2009(2): 410-415.
	YANG J X, YANG G H, SUN S X, et al.. Physiological effects of zinc sulphate on maize seed germination [J]. J. Plant Nutr. Fert., 2009(2): 410-415.
20	孙建华,李志洪,李辛,等.高量施锌肥对玉米Zn吸收和积累及产量的影响[J].水土保持学报,2012,26(4):212-215.
	SUN J H, LI Z H, LI X, et al.. Influence of high-application of Zinc fertilizers on absorption and accumulation of Zn content and maize yield [J]. J. Soil Water Conser., 2012, 26(4): 212-215.
21	鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000:335-336.
22	李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-178.
23	李超, 唐海明, 汪柯,等. 栽培模式对南方丘陵红壤旱地春玉米生理生化特性及产量的影响[J].华南农业大学学报,2016,37(4): 13-17.
	LI C H, TANG H M, WANG K, et al.. Effects of cultivation models on physiological and biochemical characteristics and yield of spring maize in hilly red soil upland of southern China [J]. J. South China Agric. Univ., 2016, 37(4): 13-17.
24	黄骥,王建飞,张红生.植物C₂H₂型锌指蛋白的结构与功能[J].遗传,2004(3): 414-418.
	HUANG J, WANG J F, ZHANG H S.Structure and function of plant C₂H₂ type zinc finger protein [J]. Hereditas, 2004(3): 414-418.
25	蔡鑫鑫,杨克军,王玉凤,等.锌肥对寒地春玉米生理生化指标的影响[J].耕作与栽培,2013(5):13-15.
	CAI X X, YANG K J, WANG Y F, et al.. Effects of zinc on physiological and biochemical indexes of spring maize in cold areas [J]. Tillage Cultiv., 2013(5): 13-15.
26	黄伟东.锌-硼喷施对寒地玉米生理特性及产量品质的影响[D].大庆:黑龙江八一农垦大学,2020.
	HUANG W D. Effects of zinc-boron spraying on physiological characteristics, yield and quality of maize in cold region [D]. Daqing: Heilongjiang Bayi Agriculture University, 2020.
27	李萌.不同栽培模式和锌肥对干旱半干旱地区夏玉米抗旱性的影响[D].杨凌:西北农林科技大学,2008.
	LI M. Effect of different cultivation patterns and zinc fertilization on the drought resistance of summer maize in arid and semiarid region [D]. Yangling: Northwest A & F University, 2008.
28	李靖,张玉喜,姜雯.不同基因型旱稻早期锌吸收与利用差异研究[J].中国农学通报,2014,30(27): 229-233.
	LI J, ZHANG Y X, JIANG W. Study on difference of zinc uptake and utilization in early stage of upland rice with different genotypes [J].Chin. Agric. Sci. Bull., 2014, 30(27): 229-233.
29	ERENOGLU B, NIKOLIC M, ROMHELD V, et al... Uptake and transport of foliar applied zinc (65Zn) in bread and durum wheat cultivar differing in zinc efficiency [J]. Plant Soil, 2002, 241: 251-257.
30	CAKMANK I, PFEIFFER W H, MCCLAFFERTY B. Bio-fortification of durum wheat with zinc and iron [J]. Cereal Chem., 2010, 87: 10-20.
31	毛晖.锌肥与水分对旱地缺锌区玉米生长及品质的影响[D].杨凌:西北农林科技大学,2013.
	KANG H. Effect of zinc fertilizer and water on maize growth and quality in zinc different area of dryland [D]. Yangling: Northwest A & F University, 2013.
32	康蓉,牛建彪,陈政仁,等.不同锌肥组合处理对玉米农艺性状和产量的影响[J].大麦与谷类科学,2019,36(4): 35-38.
	KANG R, NIU J B, CHEN Z R, et al.. Effects of different zinc-fertilizer combinations on the agronomic characters and yield of maize [J]. Barley Cereal Sci., 2019, 36(4): 35-38.
33	曹殿云.硫、锌肥对玉米生长发育、元素吸收及产量的影响[D].沈阳:沈阳农业大学,2017.
	CAO D Y. Effect of sulfur and zinc fertilizer on growth, element absorption and yield of maize [D]. Shenyang: Shenyang Agricultural University, 2017.

处理 Treatment	茎中锌素/ （mg·株^-1） Zn in leaf/ （mg·plant^-1）		叶中锌素/ （mg·株^-1） Zn in stem/ （mg·plant^-1）		锌素转移量/（mg·株^-1） Zn transfer quantity/ （mg·plant^-1）		转移率 Transport rate/%		贡献率 Contribution rate/%
处理 Treatment	抽雄期Tasseling period	成熟期Maturation period	抽雄期Tasseling period	成熟期Maturation period	茎 Stem	叶 Leaf	茎 Stem	叶 Leaf	茎 Stem	叶 Leaf
T0	1.16 f	0.8 e	2.24 f	1.38 e	0.36 e	0.86 e	30.73 b	38.22 b	44.38 b	61.86 b
T1	1.39 e	0.97 d	3.04 e	1.81 d	0.42 d	1.23 d	30.10 a	40.46 ab	43.06 b	67.97 ab
T2	1.71 b	1.15 b	4.06 d	2.41 c	0.56 b	1.65 c	32.57 a	40.73 ab	48.31 a	68.98 ab
T3	1.76 a	1.18 a	5.00 a	2.90 a	0.58 a	2.11 a	32.78 a	42.06 a	48.77 a	72.67 a
T4	1.69 c	1.14 b	4.60 b	2.74 b	0.54 b	1.86 b	32.09 a	40.47 ab	47.26 a	67.99 ab
T5	1.53 d	1.04 c	4.16 c	2.54 c	0.49 c	1.62 c	31.88 a	38.92 ab	46.81 a	63.73 ab

处理 Treatment	茎中锌素/ （mg·株^-1） Zn in leaf/ （mg·plant^-1）		叶中锌素/ （mg·株^-1） Zn in stem/ （mg·plant^-1）		锌素转移量/（mg·株^-1） Zn transfer quantity/ （mg·plant^-1）		转移率 Transport rate/%		贡献率 Contribution rate/%
处理 Treatment	抽雄期Tasseling period	成熟期Maturation period	抽雄期Tasseling period	成熟期Maturation period	茎 Stem	叶 Leaf	茎 Stem	叶 Leaf	茎 Stem	叶 Leaf
T0	1.16 f	0.8 e	2.24 f	1.38 e	0.36 e	0.86 e	30.73 b	38.22 b	44.38 b	61.86 b
T1	1.39 e	0.97 d	3.04 e	1.81 d	0.42 d	1.23 d	30.10 a	40.46 ab	43.06 b	67.97 ab
T2	1.71 b	1.15 b	4.06 d	2.41 c	0.56 b	1.65 c	32.57 a	40.73 ab	48.31 a	68.98 ab
T3	1.76 a	1.18 a	5.00 a	2.90 a	0.58 a	2.11 a	32.78 a	42.06 a	48.77 a	72.67 a
T4	1.69 c	1.14 b	4.60 b	2.74 b	0.54 b	1.86 b	32.09 a	40.47 ab	47.26 a	67.99 ab
T5	1.53 d	1.04 c	4.16 c	2.54 c	0.49 c	1.62 c	31.88 a	38.92 ab	46.81 a	63.73 ab

处理 Treatment	穗长 Ear length/cm	穗粗 Ear coarse/cm	秃尖 Bald tip/cm	行粒数 kernel number	穗行数 Row number	穗数 Ear number	穗粒数 Grain number	百粒重 100-grain weight/g	产量 Yield/ （kg·hm^-2）
T0	17.83 b	47.82 b	1.71 a	36.40 b	14.40 b	6.81 a	509.33 b	33.98 b	10 025.54 b
T1	18.58 ab	48.54 ab	1.37 ab	38.10 ab	15.00 ab	6.89 a	549.56 ab	34.82 ab	11 162.63 b
T2	19.23 ab	49.15 a	1.13 b	39.08 ab	15.40 ab	6.92 a	577.33 a	35.00 ab	11 874.61 b
T3	19.47 a	49.46 a	0.96 b	41.00 a	16.00 a	6.90 a	592.89 a	35.37 a	12 433.86 a
T4	18.67 ab	48.71 ab	1.16 b	39.80 ab	15.20 ab	6.86 a	552.22 ab	34.78 ab	11 222.41 ab
T5	18.53 ab	48.69 ab	1.67 a	38.60 ab	15.00 ab	6.80 a	516.22 b	34.64 ab	10 349.42 b

处理 Treatment	穗长 Ear length/cm	穗粗 Ear coarse/cm	秃尖 Bald tip/cm	行粒数 kernel number	穗行数 Row number	穗数 Ear number	穗粒数 Grain number	百粒重 100-grain weight/g	产量 Yield/ （kg·hm^-2）
T0	17.83 b	47.82 b	1.71 a	36.40 b	14.40 b	6.81 a	509.33 b	33.98 b	10 025.54 b
T1	18.58 ab	48.54 ab	1.37 ab	38.10 ab	15.00 ab	6.89 a	549.56 ab	34.82 ab	11 162.63 b
T2	19.23 ab	49.15 a	1.13 b	39.08 ab	15.40 ab	6.92 a	577.33 a	35.00 ab	11 874.61 b
T3	19.47 a	49.46 a	0.96 b	41.00 a	16.00 a	6.90 a	592.89 a	35.37 a	12 433.86 a
T4	18.67 ab	48.71 ab	1.16 b	39.80 ab	15.20 ab	6.86 a	552.22 ab	34.78 ab	11 222.41 ab
T5	18.53 ab	48.69 ab	1.67 a	38.60 ab	15.00 ab	6.80 a	516.22 b	34.64 ab	10 349.42 b

指标 Index	秃尖 Bald tip	穗长 Ear length	穗粗 Ear coarse	穗行数 Row number	行粒数 Kernel number	穗数 Ear number	穗粒数 Grain number per ear	百粒重 100-kernel weight
穗长Ear length	-0.209
穗粗Ear coarse	-0.448	0.711^**
穗行数Ear rows	-0.249	0.339	0.107					.
行粒数Line grain	-0.513^*	0.205	0.515^*	-0.070
穗数Ear number	-0.411	-0.034	0.243	0.245	0.413
穗粒数Kernel number per ear	-0.612^**	0.447	0.607^**	0.472^*	0.795^**	0.463
百粒重100-kernel weight	-0.493^*	0.549^*	0.667^**	0.321	0.337	0.198	0.521^*
产量Yield	-0.658^**	0.464	0.664^**	0.471^*	0.768^**	0.578^*	0.972^**	0.655^**