Effects of Arbuscular Mycorrhizal Fungi on Growth and Cadmium Enrichment of Setaria viridis Under Cadmium Stress

doi:10.13304/j.nykjdb.2023.0960

Abstract

Abstract:

In order to study the effects of arbuscular mycorrhizal（AM） fungi on plant growth under heavy metal stress， the effects of AM fungus Funneliformis mosseae on the growth， photosynthetic characteristics， Cd absorption and enrichment of Setaria viridis at different levels（0， 10， 30， 50 mg·kg^-1）of Cd were studied. The results showed that under the same level of Cd stress， AM fungi significantly increased the plant height， chlorophyll content， net photosynthetic rate， transpiration rate， stomatal conductance， apparent quantum efficiency and biomass of S. viridis. When the level of Cd was 50 mg·kg^-1， the plant height， chlorophyll content， net photosynthetic rate， transpiration rate， stomatal conductance， apparent quantum efficiency and biomass of S. viridis inoculated with AM were significantiy increased by 51%， 24%， 33%， 33%， 26%， 45% and 51%， respectively， compared with those treated without AM （P<0.05）. Under the same level of Cd stress， AM fungi significantly increased Cd contents in roots， aboveground enrichment， root enrichment and Cd enrichment coefficient of S. viridis. The level of Cd was 50 mg·kg^-1， compared with the control without inoculation， the Cd contents in roots， aboveground enrichment and root enrichment of S. viridis inoculated with AM increased by 39%， 18% and 100%， respectively， which significantly promoted the absorption and enrichment of Cd by S. viridis. Above results could provide reference for the combined repair of Cd contaminated soil by AM fungi and S. viridis.

Key words: cadmium, Setaria viridis, arbuscular mycorrhizal fungi, photosynthesis

摘要：

为研究丛枝菌根（arbuscular mycorrhizal，AM）真菌对重金属胁迫下植物生长的影响，在不同镉（Cd）水平（0 、10、30、50 mg·kg^-1）下用AM真菌摩西斗管囊霉（Funneliformis mosseae）对狗尾草进行接种处理，探讨重金属污染对宿主与AM真菌共生的影响，以及AM真菌对狗尾草生长、光合特性和Cd吸收富集的影响。结果表明，同一Cd水平胁迫下，AM真菌显著提高了狗尾草的株高、叶绿素含量、净光合速率、蒸腾速率、气孔导度、表观量子效率和生物量。当Cd水平为50 mg·kg^-1时，与未接种对照相比，接种AM处理的狗尾草株高、总叶绿素含量、净光合速率、蒸腾速率、气孔导度、表观量子效率和生物量分别显著提高51%、24%、33%、33%、26%、45%和51%（P<0.05）。同一Cd水平胁迫下，AM真菌显著增加了狗尾草根部Cd含量、地上部Cd富集量、根部Cd富集量及Cd富集系数（P<0.05）。当Cd为50 mg·kg^-1时，与未接种对照相比，接种AM处理的狗尾草根部Cd含量、地上部Cd富集量、根部Cd富集量分别提高39%、18%、100%，显著促进了狗尾草对Cd的吸收和富集。研究结果为AM真菌-狗尾草联合修复Cd污染土壤提供参考。

关键词: 镉, 狗尾草, 丛枝菌根真菌, 光合作用

CLC Number:

S182

Xin ZHAO, Zilong WU, Chao HAN, Hao ZHANG, Wei SONG, Ziyi LI. Effects of Arbuscular Mycorrhizal Fungi on Growth and Cadmium Enrichment of Setaria viridis Under Cadmium Stress[J]. Journal of Agricultural Science and Technology, 2025, 27(5): 193-202.

赵昕, 吴子龙, 韩超, 张浩, 宋炜, 李子怡. 丛枝菌根真菌对镉胁迫下狗尾草生长及镉富集的影响[J]. 中国农业科技导报, 2025, 27(5): 193-202.

Figures/Tables 7

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处理 Treatment	菌根侵染率 Mycorrhizal infection rate/%		株高 Plant height/cm	生物量/（g·盆^-1） Biomass/（g·pot^-1）
处理 Treatment	Cd处理前 Before Cd treatment	Cd处理后 After Cd treatment	株高 Plant height/cm	地上部 Aboveground	根 Root	全株 Whole plant
NM0	0.00±.0.00	0.00±0.00 d	41.93±1.12 b	19.40±0.62 b	12.77±0.65 b	32.17±1.26 b
NM10	—	0.00±0.00 d	41.50±0.80 b	18.57±0.57 b	11.87±0.65 bc	30.43±0.76 bc
NM30	—	0.00±0.00 d	30.50±1.35 c	10.47±0.40 d	8.13±0.40 c	18.60±0.80 c
NM50	—	0.00±0.00 d	20.80± 0.56 d	6.33± 0.06 e	5.50±0.36 d	11.83±0.42 d
AM0	43.80±1.87	53.70±1.28 a	53.47±0.78 a	26.50±1.59 a	15.83±0.40 a	42.33±1.96 a
AM10	—	52.60±0.82 a	52.70±1.73 a	25.30±1.31 a	15.63±0.42 a	40.93±1.42 a
AM30	—	38.80±1.59 b	40.57±1.19 b	17.13±0.85 c	11.23±0.85 b	28.37±1.68 c
AM50	—	25.40±0.89 c	31.50±1.11 c	10.00±0.36 d	7.90± 0.46 c	17.90±0.82 c
菌根菌AM	**	**	**	**	**	**
镉Cd	**	**	**	**	**	**
菌根菌×镉AM×Cd	**	**	ns	**	ns	*

处理 Treatment	菌根侵染率 Mycorrhizal infection rate/%		株高 Plant height/cm	生物量/（g·盆^-1） Biomass/（g·pot^-1）
处理 Treatment	Cd处理前 Before Cd treatment	Cd处理后 After Cd treatment	株高 Plant height/cm	地上部 Aboveground	根 Root	全株 Whole plant
NM0	0.00±.0.00	0.00±0.00 d	41.93±1.12 b	19.40±0.62 b	12.77±0.65 b	32.17±1.26 b
NM10	—	0.00±0.00 d	41.50±0.80 b	18.57±0.57 b	11.87±0.65 bc	30.43±0.76 bc
NM30	—	0.00±0.00 d	30.50±1.35 c	10.47±0.40 d	8.13±0.40 c	18.60±0.80 c
NM50	—	0.00±0.00 d	20.80± 0.56 d	6.33± 0.06 e	5.50±0.36 d	11.83±0.42 d
AM0	43.80±1.87	53.70±1.28 a	53.47±0.78 a	26.50±1.59 a	15.83±0.40 a	42.33±1.96 a
AM10	—	52.60±0.82 a	52.70±1.73 a	25.30±1.31 a	15.63±0.42 a	40.93±1.42 a
AM30	—	38.80±1.59 b	40.57±1.19 b	17.13±0.85 c	11.23±0.85 b	28.37±1.68 c
AM50	—	25.40±0.89 c	31.50±1.11 c	10.00±0.36 d	7.90± 0.46 c	17.90±0.82 c
菌根菌AM	**	**	**	**	**	**
镉Cd	**	**	**	**	**	**
菌根菌×镉AM×Cd	**	**	ns	**	ns	*

处理 Treatment	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b	总叶绿素 Total chlorophyll	类胡萝卜素 Carotenoids
NM0	1.42±0.02 b	0.29±0.01 ab	1.71±0.03 c	0.56±0.02 b
NM10	1.40±0.02 b	0.28±0.01 b	1.68±0.03 c	0.54±0.01 b
NM30	1.22±0.04 c	0.21±0.02 d	1.43±0.05 d	0.44±0.01 d
NM50	1.00±0.03 d	0.13±0.02 e	1.13±0.03 e	0.36±0.02 e
AM0	1.75±0.02 a	0.33±0.02 a	2.08±0.02 a	0.63±0.02 a
AM10	1.70±0.02 a	0.31±0.01 ab	2.01±0.02 b	0.62±0.01 a
AM30	1.42±0.02 b	0.26±0.01 c	1.67±0.03 a	0.55±0.01 ab
AM50	1.20±0.03 c	0.19±0.02 d	1.40±0.04 d	0.47± 0.01 c
菌根菌AM	**	**	**	**
镉Cd	**	**	**	**
菌根菌×镉 AM×Cd	**	ns	**	*

处理 Treatment	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b	总叶绿素 Total chlorophyll	类胡萝卜素 Carotenoids
NM0	1.42±0.02 b	0.29±0.01 ab	1.71±0.03 c	0.56±0.02 b
NM10	1.40±0.02 b	0.28±0.01 b	1.68±0.03 c	0.54±0.01 b
NM30	1.22±0.04 c	0.21±0.02 d	1.43±0.05 d	0.44±0.01 d
NM50	1.00±0.03 d	0.13±0.02 e	1.13±0.03 e	0.36±0.02 e
AM0	1.75±0.02 a	0.33±0.02 a	2.08±0.02 a	0.63±0.02 a
AM10	1.70±0.02 a	0.31±0.01 ab	2.01±0.02 b	0.62±0.01 a
AM30	1.42±0.02 b	0.26±0.01 c	1.67±0.03 a	0.55±0.01 ab
AM50	1.20±0.03 c	0.19±0.02 d	1.40±0.04 d	0.47± 0.01 c
菌根菌AM	**	**	**	**
镉Cd	**	**	**	**
菌根菌×镉 AM×Cd	**	ns	**	*

处理 Treatment	净光合速率 P_n/（μmol·m^-2·s^-1）	蒸腾速率 T_r/（mmol·m^-2·s^-1）	气孔导度 G_s/（mol·m^-2·s^-1）	胞间CO₂浓度 C_i/（μmol·mol^-1）
NM0	11.00±0.36 b	1.70±0.04 b	159.33±1.53 b	257.33±1.53 d
NM10	10.63±0.15 bc	1.66±0.03 b	157.00±3.00 b	260.67±2.52 d
NM30	9.43±0.50 c	1.39±0.05 c	132.33±3.06 c	273.00±1.00 c
NM50	7.63±0.15 d	1.06±0.04 d	104.33±4.16 d	313.33±4.16 a
AM0	12.97±0.60 a	1.97±0.06 a	181.67±6.03 a	233.67±2.52 d
AM10	12.47±0.55 a	1.92±0.05 a	181.33±3.05 a	238.67±2.08 d
AM30	11.10±0.26 b	1.69±0.02 b	153.00±4.00 b	257.67±4.04 d
AM50	10.13±0.29 bc	1.41±0.09 c	131.77±4.73 c	284.33±6.43 b
菌根菌AM	**	**	**	**
镉Cd	**	**	**	**
菌根菌×镉 AM×Cd	ns	ns	ns	*