Table of Content

15 August 2014, Volume 16 Issue 4

Previous Issue    Next Issue

Review of Studies on Innovation System of Agricultural Science and Technology in China

JIANG He\|ping1, LIU Xue\|yu2

2014, 16(4):  1-9.  DOI: 10.13304/j.nykjdb.2014.418

Asbtract ( )   PDF (1046KB) ( )  

Related Articles | Metrics

As an important suport for developing agriculture in China, agricultural science and technology innovation is a key link for realizing the strategy of developing agriculture by  science and technology. This paper seriousely sorted the recent research progress about agricultural science and technology innovation from domestic scholars, and carried out systematic analysis in 4 aspects including agricultural technology innovation, innovation system of agricultural science and technology, innovative ability and efficiency of agricultural science and technology, innovative subject of agricultural science and technology. Then, the paper summarized its views about agricultural science and technology innovation research and reviewed the research achievements. On this basis, the paper put forward  characteristics, deficiencies and problems existing in this research area.

Technological Innovation of Agricultural Information Service in the Age of Big Data

LI Xiu\|feng, CHEN Shou\|he, GUO Lei\|feng

2014, 16(4):  10-15.  DOI: 10.13304/j.nykjdb.2014.363

Asbtract ( )   PDF (1239KB) ( )  

Related Articles | Metrics

Agricultural data is a kind of typical big data. If big data technology is applied in the field of agriculture information service, it will not only bring revolutionary progress for the agricultural information technology service, but also can promote the overall progress of agricultural industry. This paper summarized several problems for agricultural information service relating big data. We regarded that agricultural big data requires technological innovation of agricultural information service, mainly including three aspects: intelligent processing technology of agricultural big data, decision ontology technology of agricultural big data, human\|machine interactive technology of agricultural informationization cloud service. And the technology schemes and key research tasks were provided for each of the three aspects. The development and application prospects of big data on the field of agricultural information service are bright, which will accelerate the agricultural informationization, and promote the effective combination of industry\|university\|research, and is expected to realize breakthroughs in technology and application soon. National agricultural big data sharing alliance should be established, and big data\|based agricultural information service demonstrations should be carried out. It is necessary to support the fundamental researches vigorously by public funds.

Balance and Trend Analysis Between Supply and Demand of Barley in China

ZHANG Lin1, LI Xian\|de1, SUN Dong\|sheng2*

2014, 16(4):  16-22.  DOI: 10.13304/j.nykjdb.2014.068

Asbtract ( )   PDF (1331KB) ( )  

Related Articles | Metrics

This paper analyzed barley production, consumption and trade condition in different uses by using food balance sheet method, based on FAO statistics and data released by National Grain and Oil Information Center. The result showed: Chinese barley supply composed by domestic production and imports, both of them were equal. Chinese barley had high import dependence. Beer barley was accounting for a large proportion in Chinese barley consumption therefore food and feed barley consumption were relatively less. China barley trade was dominated by imports. Barley import was sensitive to the domestic market price, international market price and domestic beer barley demand, Trade environment and policy have small impact on barley imports.

Construction Path on Ecological Sustainability of Agricultural Resources in China

SHEN Yu\|jun, ZHAO Li-xin, MENG Hai-bo*

2014, 16(4):  23-33.  DOI: 10.13304/j.nykjdb.2013.619

Asbtract ( )   PDF (1245KB) ( )  

Related Articles | Metrics

Due to the rapid economy progress and the intensive agriculture development, the problems still exist in China  such as utilization for water, soil, fertilizer and other agricultural resources, and serious ecological and environmental pollution etc.. The domestic scholars have carried out some studies on the efficient utilization of agricultural resources and ecological protection mode at specific areas. However, these studies were incomplete and lack of systematicness. This paper put forward the concept of ecological sustainability of agricultural resources and its construction path; summarized 5 factors affecting the establishment of ecological sustainability of agricultural resources, namely regional differences, industrial structure, economic development, policy conditions and technical support. The construction path included core model, supporting technology, executor, organization form, operating mechanism. On the basis of the influencing factors and building up the construction path, this paper analyzed the function of each factor and their interaction; clarified that the path was constructed by taking the applicable model as the core, key technology as support, and carried out through specific organization form and mechanism under the pushing of executor. The paper also summarized the construction paths of ecological sustainability of agricultural resources for different regions, and provided references for the government and the public to understand the operation situation and development of this construction path.

Expression Regulation of DREB Transcription Factor

GUO Yi\|liang1,2, DUAN Liu\|sheng1*, HUANG Rong\|feng2

2014, 16(4):  34-40.  DOI: 10.13304/j.nykjdb.2013.542

Asbtract ( )   PDF (1177KB) ( )  

Related Articles | Metrics

Plant DREB transcription factor, one subfamily member of AP2/ERF family, can specially bind to DRE/CRT cis\|acting element. They play important roles in plant resistant to abiotic stresses, such as cold, drought, salt, through regulating the expression of stress\|responsive genes. This paper reviewed the regulating and controlling role of DREB transcription factor, emphasized in introducing the research findings made in regulating and controlling mechanism during the process of its expression, function, degradation; and also prospected the future orientation for transcription factor research.

Origin, Structure and Function of Plastid-associated Fibrillin Protein Family in Plants

ZHOU Hui-mei, XIE De-ying, ZOU Juan-zi, LI Jia-jia, XIE Guo-sheng*

2014, 16(4):  41-49.  DOI: 10.13304/j.nykjdb.2013.462

Asbtract ( )   PDF (4522KB) ( )  

Related Articles | Metrics

Plastid is an important organelle for photosynthesis and storing organic matter in plant cells. Fibrillins are the nuclear gene-encoding plastid lipid\|binding proteins originated from cyanobacteria and are prevalent in plastids of plants. Their molecular weight and pI are 21~42 kDa and 4~9, respectively. The sequence of fibrillins contains amino\|terminal transit peptides, lipid\|binding motif DLDKLQGKWRLLY, cellular adhesion motif RGD, and a protein kinase C domain. Previous reports have showed that fibrillins can improve the structural stability of plasmids under adverse environmental stresses, regulate the biosynthesis of plastoquinone (PQ), triacylglycerol (TAG) and jasmonic acid (JA), mediate the stress hormone abscisic acid (ABA) signal transduction pathways in the stress tolerance, and more importantly in plant growth, development and stress tolerance. In this study, fibrillin family genes have been reviewed in their protein structure and genetic functions in higher plants. The future key issues and research directions in this field have also been proposed.


Research Advances in Plant Saponins Biosynthesis and Its Regulation

LEI Cai-yan, LI Jing-jing, YAN Feng-ming*

2014, 16(4):  50-58.  DOI: 10.13304/j.nykjdb.2014.033

Asbtract ( )   PDF (1252KB) ( )  

Related Articles | Metrics

Saponins are a group of secondary metabolites occurring constitutively in most higher plant species, which have wide potential application in development of medicine, plant\|derived pesticides, health  products and nourishment. However, the contents of saponins are low and unstable in plants, which seriously affects the development and utilizations of these compounds. Therefore, regulation biosynthesis of saponins and improvement of its production have attracted more and more attention. This paper expounded the biosynthetic pathway, cultivation technology, genetic engineering, cell engineering and hairy root cultivation, and the research progress made in its synthesis of regulation. In addition, this paper also summarised the problems existing in this area and prospects the future research orientation.

Progress on Plant Heavy Metal Cadmium Resistance and Accumulative Mechanism Based on Mutant

DENG Xiao\|juan, HUANG Yi\|an, WANG Peng, FANG Xiao\|long, ZHANG Jie, YANG Cun\|

2014, 16(4):  59-64.  DOI: 10.13304/j.nykjdb.2014.131

Asbtract ( )   PDF (1011KB) ( )  

Related Articles | Metrics

Cadmium (Cd) is one of the toxic pollutants, which can not only influence plant growth and development, but also threat human health by cadmium accumulation in plants through food chain. Mutant is an important resource for crop improvement and functional genomics research. This paper summerized the research work and achievemrnt by using various mutants to the molecular mechanism of plant cadmium resistance and accumulation. Based on these studies, the paper put forward the strategy of carring out research on crop cadmium resistance, low\|accumulation mechanism, and molecular breeding by using mutants, so as to provide theoretical guidance for breeding crop cultivars with high cadmium resistance and low\|accumulation.

Progress on Molecular Biology of Salinity Tolerance in Sorghum

HAN Yu\|cui1, YE Kai2, HOU Sheng\|lin1, TU Zhen\|dong2, LV Peng1, DU Rui\|heng1,

2014, 16(4):  65-70.  DOI: 10.13304/j.nykjdb.2014.026

Asbtract ( )   PDF (985KB) ( )  

Related Articles | Metrics

Salinity stress is a main factor affecting plant growth and crop yield. It is a simple and effective way to solve salt damage by genetic improvement. This paper reviewed the salt\|tolerant germplasm resources, localization of salt\|tolerant genes (QTLs), cloning, transferring and utilization of salt\|tolerant genes through gene engineering technology. The paper also prospected the future of research  on sorghum and salt tolerance, so as to provide theoretical basis for genetic improvement of salt\|tolerant ability of sorghum and other crops. It could also solve the salinity stress problem, limitting crop yield and quality in agricultural production.

Cloning and Analysis of Anti\|stress Transcription Factor Gene
HhDREB2 from Halimodendron halodendron. Voss

LEI Zhi1, YIN Cui\|cui2, LI Yong\|liang3, SUN Zhan\|min1, WU Yan\|min1*

2014, 16(4):  71-78.  DOI: 10.13304/j.nykjdb.2014.083

Asbtract ( )   PDF (7072KB) ( )  

Related Articles | Metrics

In this study, a full\|length cDNA sequence of DREB transcription factor gene, HhDREB2 (Genbank No.: EU872018), was isolated from Halimodendron halodendron. Voss by homology\|based cloning and RACE (rapid amplification of cDNA end) technology. Sequence analysis showed that the cDNA sequence is 873 bp without introns and has a maximum open reading frame from 108 to 626 bp, encoding 172 amino acid residues. Molecular weight of the gene encoding protein is 19.702 kDa, isoelectric point is 8.92. Phylogenetic tree and homologous sequence alignment analyses indicated that this protein contained a typical AP2/ERF conservative domain, and was most closely related to soybean GmDREB2, belonging to the A5 subgroups. Yeast one\|hybrid experiment demonstrated that HhDREB2 gene encodes a transcription activator. In addition, subcellular localization analysis of a construct with the transient expression vector of the HhDREB2 gene showed that the HhDREB2 protein was localized in the nucleus. The expression level of HhDREB2 gene was greatly induced by high salt, drought and low temperature through semi\|quantitative RT\|PCR, and had no significant change with GA3, NAA, ABA and 6BA treatments.

Research Progress on the Function and Regulatory Mechanism of CsrA, a Global Regulator in Bacteria

SHANG Li\|guo, ZHAN Yu\|hua, GONG Pai, YAN Yong\|liang*

2014, 16(4):  79-86.  DOI: 10.13304/j.nykjdb.2014.172

Asbtract ( )   PDF (2834KB) ( )  

Related Articles | Metrics

CsrA was initially found and named during research on the carbon storage regulatory system. CsrA affects stability and translation of target genes by binding mRNA as a widespread post\|transcriptional regulator in Enterobacterium, Pseudomonas, Bacillus and other microorganisms. Studies indicated that CsrA not only regulated a number of physiological processes such as central carbon metabolism, motility, biofilm formation, quorum sensing, and pathogenicity, but also influenced the synthesis of many secondary metabolites. Recent research showed that the functions of CsrA were strictly related to two non\|coding RNAs; these three genes coordinated to form a complex system. This review introduced the various functions of CsrA in different bacteria and its regulatory mechanism, information that will be useful for learning more about the regulatory mechanism of the carbon storage system and alter it for future studies.

Effect of Bt Transgenic Corn Straw Fermentation on SFC\|2 Microbial Community

MENG Yao1, GU Wan\|rong2, LI Jing2, WEI Shi2*, YANG De\|guang2

2014, 16(4):  87-94.  DOI: 10.13304/j.nykjdb.2014.055

Asbtract ( )   PDF (2104KB) ( )  

Related Articles | Metrics

Nowadays, genetic engineering make it possible for maize with insect resistance by transferring Bt gene. Satety and effects of  Bt transgenic maize in its influence on the composite microbial system of SFC\|2 during the fermentation process was studied. The change of pH, the speed of cellulose decomposing, the qualitative and quantitative analysis of decomposing outcome (GCMS), the content of Bt toxin were surveyed entirely. DGGE (degeneration grad gel electrophoresis) method was applied to provide a test in molecule level microorganism genetic material in the process of fermentation. The effect of SFC\|2 on straw fermentation was described by comparing the difference between the transgenic corn and non\|transgenic corn. The results showed that the lactobacillus community SFC\|2 was inoculated into the corn straw and its fermentation effects were good. The smell of fragrance was also good. The quality of material was loose and the luster was bright. In the initial period, pH value dropped quickly and reached the minimum value at the 7th day, then increased slowly. There is no great significant pH value difference between transgenic and non\|transgenic corn. Soluble sugar content showed the same trend with pH value, which reached the lowest value in the 13th day. There was also no great significant difference. Except the crude protein content increased a little, the content of crude fat and ash did not change during the fermentation time. The differences between these characteristics were also not significant. The measurement of Bt toxin content showed that the 2 transgenic corns descended significantly in the 7th day. Bt toxin content reduced gradually during the whole fermentation period. GCMS showed that there were no great differances in these 4 corns, such as the content of ethanol, acetic acid, lactic acid and glycerin. Our results could confirm that Bt transgenic maize has no positive effect on microorganism, but offer the means to evaluate the potential non\|target effects of transgenic crops. Hence, this study has provided preliminary evidence for the rational use and safety evaluation of Bt transgenic maize.

Studies and Analysis on Agricultural Machinery Development in China Based on Factor Analysis

HUANG Yong-bao1, JI Yan-guang2*

2014, 16(4):  95-101.  DOI: 10.13304/j.nykjdb.2014.127

Asbtract ( )   PDF (1165KB) ( )  

Related Articles | Metrics

Under the background of putting forward land transfer and moderate scale land management by the government, based on the previous consecutive 23 years data, this paper carries out an empirical analysis about several hypothetical indicators that may be correlated to the development of agricultural mechanism, and extracts the factors affecting the development of agricultural machinery, using factor analysis method. Then regression analysis is conducted to find out some rules of  agricultural machinery development in China. The results showed that in the next few years, agricultural machinery in China will develope even faster. Agricultural machinery with intelligent, high power, high efficient environment protection will replace the aging and energy consuming  machinery. Before ending, the paper gives some policy suggestions and provides certain meaningfull references for the development of agricultural machinery in China.

Effect of Different Packing on Quality Changes of Hot Boning Beef During Storage

LIU Fei1,2, LEI Yuan\|hua1, ZHANG Wen\|jie1, SHA Kun1, LI Hai\|peng1, ZHANG Zhi\

2014, 16(4):  102-108.  DOI: 10.13304/j.nykjdb.2014.224

Asbtract ( )   PDF (1319KB) ( )  

Related Articles | Metrics

People has been paid more concern  to the packing way of hot\|boning beef and how to preserve beef quality during storage. This research studied the quality changes of hot boning beef during storage under 4℃ with different packaging methods, and tested the quality related characteristics with different storaging times. The results showed that the loss difference of 0~14 d vacuum heat shrink packaging and film packaging was remarkable (P<005), and the changing trend of water loss rate in beef was similar, while the water loss rate in film packaging was higher than that in vacuum heat shrink packaging. The beef tenderness of these 2 methods was all improved. The beef  L* of film packaging was higher than that of the other one. Beef  a* of film packaging dropped significantly （P<005）, while b* increased remarkably（P<0.05） after 72 h. The total aerobic bacteria count in film packaging was beyond the national health standards and that in vacuum heat shrink packaging showed an increasing trend, but did not exceed the national health standards during 72 h~14 d period. Therefore  vacuum heat shrink packaging could better guarantee the quality of hot boning beef and its shelf life is longer than that of film packaging.

Marginal Land Resources Development and Biomass Energy Research in Yellow River Delta

WU Cong\|wen1,2, CHEN Xiao\|bing2*, WEI Cai\|bo3, XU Hua\|ling5, ZHANG Li\|bin5,

2014, 16(4):  109-119.  DOI: 10.13304/j.nykjdb.2014.034

Asbtract ( )   PDF (1463KB) ( )  

Related Articles | Metrics

The Yellow River Delta has abundant reserve of land resources. Combining the marginal land development with biomass energy development is conducive to the rational utilization of land resources and the efficient and sustainable supply of biomass feedstock in this area. On the basis of depth analysis of soil and water resources and rural biomass energy utilization status in the Yellow River Delta, combining the domestic and international present situation on biological energy research and the latest progress, this paper discussed the necessity and feasibility of large\|scale cultivation of energy plants like sweet sorghum, jerusalem artichoke. ricinus communis and switchgrass in the saline alkali land, and proposed several key suggestions to promote the biomass energy development and marginal land utilization from the technical, policy, market, etc.,  which study has guiding significance for the scientific development of land and water resources in the Yellow River Delta.

Progress and Perspectives of Nitrogen Rhizodeposition in Crops

ZANG Hua\|dong1, QIAN Xin1, YANG Xue\|chao1, REN Chang\|zhong2, ZENG Zhao\|hai1,

2014, 16(4):  120-126.  DOI: 10.13304/j.nykjdb.2014.046

Asbtract ( )   PDF (1141KB) ( )  

Related Articles | Metrics

Nitrogen (N) rhizodeposition is an important source of available nutrient for soil microorganisms, and also a crucial connection between plant, soil and microorganism. Recently, along with the in\|depth studies on crop above\|ground parts, increasing attention has been paid to crop below\|ground parts, and N rhizodeposition is becoming one of the most important research fields. This paper briefly presented the latest research progress on N rhizodeposition both at home and abroad, including quantity assessment, influence factors, research and calculate methods, etc. for reference of relative research. Additionally, the paper put forward suggestions as strengening in the future the studies on rhizosphere sediment nitrogen and composition under field in\|situ conditions, getting a better understanding of N rhizodeposition regulatory mechanism, and its synergy and mechanism with carbon rhizodeposition.

Effects of Nitrogen Fertilizer on Different Japonica Rice Varieties Planted in the Hilly Land of Central Hubei Province

HOU Wen\|feng1, LI Xiao\|kun1*, ZHUANG Guang\|quan2, GUO Hong\|yan2, YUAN Ying\|

2014, 16(4):  127-132.  DOI: 10.13304/j.nykjdb.2014.017

Asbtract ( )   PDF (1122KB) ( )  

Related Articles | Metrics

A field trial was conducted in Dangyang City to compare the yield and nitrogen (N) use efficiency of 2 japonica rice varieties (Zhongdao 1 and Huaidao 5) in the hilly land of central Hubei Province (single\|cropping indica rice area) under different N levels. The results showed that the grain yield of the treatments with N were increased averagely by 2 179 kg/hm2 and 3 209 kg/hm2, and the increase rates were 45.6% and 53.5%, respectively.  Linear and platform correlations had been shown between yield and N fertilizer amount. The platform yields of “Zhongdao 1” and “Huaidao 5” were 7 517 kg/hm2 and 10 234 kg/hm2, respectively. The most suitable N application amount were 221.0 kg/hm2 and 235.5 kg/hm2, respectively. Compared with N0 treatment, the N uptakes by these 2 japonica rice varieties were averagely increased  58.7 kg/hm2 and 71.8 kg/hm2, and the increase rates were 80.9% and 85.2%. When the N application amount was 248 kg/hm2, the N recovery efficiency (NRE), N agronomic efficiency (NAE), N contribution rate (NCR) and partial factor productivity of applied N (PFPN) of the 2 varieties were 29.4% and 37.6%, 10.6 kg/kg and 18.0 kg/kg, 35.4% and 42.6%, 29.8 kg/kg and 42.3 kg/kg. The result of comprehensive analysis  believed that “Huaidao 5” could get a higher yield and high N use efficiency when planted in the hilly land of central Hubei Province.

Effects of Spraying Uniconazole and Seeding Age on Seedling Quality and Grain Yield in Paddy Field for Mechanical Transplanting Rice

WANG Bing1,2, WU Wen\|ge1*, ZHOU Yong\|jin1, CHEN Gang1, XU You\|zun1, LI Xia\|h

2014, 16(4):  133-142.  DOI: 10.13304/j.nykjdb.2014.100

Asbtract ( )   PDF (3880KB) ( )  

Related Articles | Metrics

To invest the effect of vniconazle on mechanical transplanting  rice. This study took Huiliangyou 6 as experimental material and planted at seedling raising plates with different treatments of spraying uniconazole in 1.5 leaf age, and then 4 different age seedlings were transplanted into paddy field. The seedling quality, transplanting quantity and grain yield were tested. Results indicated as follows: ① in  serious over\|optimum stage of mechanical transplanting rice seedling, the comprehensive seedling quality decreased rapidly, i.e. the plant height increased, the MDA content in seedling leaf blades increased, while roots twisting power and root vigor decreased. All these eventually led to the decrease of transplanting quantity and seedlings revived slowly after transplanting. The grain yields of 25 d, 30 d and 35 d seedling age reduced by 1 403.55 kg/hm2，1 995.75 kg/hm2 and 2 920.95 kg/hm2, respectively, compared with that of 20 d seedling age; ② seedling quality, transplanting quantity and grain yield were significantly increased among treatments with spraying uniconazole, i.e. decrease of plant height, MDA content in seedling leaf, miss planting rate, etc. and increase of base stem width, number of green leaves, roots twisting power and root vigor, etc.. Compared with the control, the rice yield increased by 9%~32%; ③ the effects of spraying uniconazole with different concentrations on different transplanting seedling age were different. In optimum stage, there was no significant difference between different concentrations, but in the over\|optimum and over\|optimum stage, uniconazole spraying effect was enhanced with increasing concentration.

omprehensive Evaluation of Tobacco Planting Soil Based on
Multi-objective Decision-making and Tobacco Chemical Component

ZHENG Dong\|fang1, HUANG Hua\|gang2, XU Zi-cheng1, ZHAI Xin2, CHEN Xue2

2014, 16(4):  143-150.  DOI: 10.13304/j.nykjdb.2014.093

Asbtract ( )   PDF (1133KB) ( )  

Related Articles | Metrics

\|cheng1, ZHAI Xin2, CHENG Xue2


(1.College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002;
2.Bijie Branch of Guizhou Provincial Tobacco Company, GuizhouBijie 551700, China)

In order to comprehensively evaluate the quality of tobacco grown on different soil type and soil texture, this study collected 149 soil samples including 5 soil types and 8 kinds of soil texture and the corresponding 149 Yunyan No.85 (C3F) tobacco leaf samples in Bijie tobacco\|growing areas, and carried out comparative analysis on 10 chemical components of tobacco leaf. Then multi\|objective decision\|making method based on entropy weight was adopted to comprehensively evaluate these chemical components. The results showed that among 10 chemical component indicators the diferences among 6 indicators were extremely significant among soil types, 5 indicators had extremely significant differences among soil textures, and one indicator was significantly different. Each chemical component indicator had different expression among soil type and doil texture.  The result of comprehensive evaluation indicated that the expressions of 5 soil types were purple soil>yellow soil > yellow brown soil> lime soil soil>fluvo\|aquic soil; the expression of 8 species of soil textures were powder soil> sticky soil> clay> silty clay >loam clay> loam> sticky silty loam> sandy clay loam.

Feedback of Ageratina adenophora Soil Microbe on A. adenophora and Native Plants

XIAO Bo1§, ZHOU Wen1§, LIU Wan\|xue1*, JIANG Zhi\|lin2, WAN Fang\|hao1

2014, 16(4):  151-158.  DOI: 10.13304/j.nykjdb.2014.069

Asbtract ( )   PDF (1815KB) ( )  

Related Articles | Metrics

The competitive succession of plants community is determined by feedback of interaction between plant and soil microbe. In order to confirm the role of soil microbe in the population establishment and expansion of Ageratina adenophora Sprengel, a vicious invasive weed in china,  the feedback effects of microorganisms in plant rhizosphere soil on growth of Ageratina adenophora Sprengel and 2 native plants were compared, Eupatorium lindleyanum DC. and Setaira viridis (L.) Beauv, by conducting the greenhouse pot experiment. A. adenophora and native plant soil were sterilized, added fungicide and no treated (control groups), respectively. The results showed that soil microorganisms in plant rhizosphere can produce a direct positive feedback effects on the growth of plants. The biomass of A. adenophora, E. lindleyanum and S. viridis decreased significantly after adding fungicide or sterilizing. There were no significant difference of AMF root colonization of E. lindleyanum and S. viridis between A. adenophora soil and native plant soil in control group, but AMF root colonization of E. lindleyanum and S. viridis in A. adenophora soil increased by 81.02% and 89.7% compared to native plant soil after adding fungicide, respectively. The PLFAs results also showed that the soil microbe communities of A. adenophora and native plants were apparently different. It was considered that the invasive plant, A. adenophora produced positive feedback to itself by affecting the soil microbe communities and function in invaded habits, subsequently contributing to its growth and population expansion.

Research Progress on Vibrio harveyi and its Main Pathogenic Factors

LI Yang, LI Qiang*, ZHANG Xian\|yu

2014, 16(4):  159-166.  DOI: 10.13304/j.nykjdb.2014.031

Asbtract ( )   PDF (1184KB) ( )  

Related Articles | Metrics

Vibrio harveyi has been widely studied as the main pathogenic bacteria in aquaculture. This paper briefly expounded the hosts of V. harveyi and the symptoms caused by V. harveyi. It also summarized the research progress made in its pathogenic factors, the detecting techniques and control method, so as to provide reference for further studies on pathogenesis of V. harveyi.

Studies on Character of a Chitinase ChiC from Aeromonas veronii B565 and its Synergism with Chitin Binding Protein

HUO Feng\|min§, YANG Ya\|lin§, XU Li, ZHANG Mei\|chao, HE Su\|xu, ZHOU Zhi\|gang

2014, 16(4):  167-175.  DOI: 10.13304/j.nykjdb.2014.125

Asbtract ( )   PDF (3230KB) ( )  

Related Articles | Metrics

To investigate the characteristics of low activity ChiC from A. veronii B565 and break the bottleneck of its application, this experiment established a recombined ChiC pichia engineering bacteria GS115/PIC9\|ChiC, carried out methanol induction expression and purification, and studied its basic enzymology properties. The result indicated that  ChiC was expressed in P. pastoris with a yield of 232.6 mg/L after 48 h methanol induction. The purified ChiC showed optimal specific activity of 7.6 U/mg at pH 8.0 and 40℃.  ChiC had broad adaptability for pH and temperature. It retained over 80% optimal activity at pH 3.0~10.0 at 40℃ or the temperature 0~40℃ at pH 7.0. The chitin binding protein CBP21 from Serratia marcescens GIM1.217 was expressed in E. Coli. The affinity of CBP21 for shrimp shell chitin and colloidal chitin were different, the chitinase activity of ChiC was increased significantly from 2 (colloidal chitin as substrate) to 9 (shrimp shell chitin as substrate) fold, when acted synergistically with CBP21. This study laid a theoretical foundation for further studying chitinase gene, and provided a possible solution for breaking the bottleneck of this low enzyme activity of chitinase application.

Establishment and Application of Sandwich ELISA Method for Detecting Avian Beta-defensin 6

LIANG Rui1, LIANG Ying1, QI Ke-zong1, WANG Ai-ronng2, PENG Kai-song1*

2014, 16(4):  176-180.  DOI: 10.13304/j.nykjdb.2014.084

Asbtract ( )   PDF (1119KB) ( )  

Related Articles | Metrics

To detect avian beta-defensin 6 (AvBD6) quantitatively, a sandwich ELISA method was established and applied to detect the  concentration of AvBD6 in serum. The results showed that the optimal dilution of capture antibody (mouse anti\|B cell linear epitopes of AvBD6 polyclonal antibody), detecting antibody (rabbit anti\|N\|terminal of AvBD6 polyclonal antibody) and horseradish peroxidase (HRP)\|conjugated goat anti\|rabbit IgG was 2 500, 2 000 and 10 000, respectively. The standard curve regression equation was y=12 024x-2 583, (R2=0.984 2) in the linear detection range from 20 to 320 ng/mL. The serum AvBD6 concentration of control group was slightly higher than that of ACTH group(P>0.05) after 1 day of ACTH (25 IU/kg BW) injection, but the AvBD6 concentration of the control group was slightly lower than that of ACTH group(P>0.05) after 5 d of ACTH injection; and the effect was not significant. The serum AvBD6 concentration of infected group after 24 h of Escherichia coli exposure or 3 h and 24 h of salmonella exposure were significantly higher than that of the control group (P<0.05). Our results indicated that AvBD6 involved in the resistance to system infection of Escherichia coli and salmonella.