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Table of Content

    15 November 2018, Volume 20 Issue 11
    Research Progress in Unintended Effects of Genetically Modified Crops on Intestinal Tract of Livestock and Poultrys
    ZHANG Lilan, CHEN Liang, ZHANG Hongfu*
    2018, 20(11):  1-13.  DOI: 10.13304/j.nykjdb.2017.0841
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    With the development of genetic engineering technology, the global planting areas for genetically modified (GM) crops are increasing sustainably. More than 75% of GM crops and their by-products are used as animal feed. It is very necessary to evaluate the safety of GM crops. Intestinal tract is the largest organ in immune system and plays an important role in maintening normal physiological functions of animals. Studies on unintended effects of GM crops on intestinal tract are the hot spots in safety assessment of GM crops as animal feed. This paper introduced the particularity and necessity of safety evaluation of GM crops as animal feed; analyzed the potential digestion and transfer rules of the transgenic ingredients in animal feed; and summarized the research progress on unintended effects of GM crops in intestinal morphology and microecology balance of the livestock and poultry, which was expected to provide references for the establishment and perfect of GM crops evaluation system.
    Study on the Operating Mechanism of CGIAR CRPs and the Cooperation with China
    HAN Xinru, YAN Yan, WANG Xiudong*
    2018, 20(11):  14-21.  DOI: 10.13304/j.nykjdb.2018.0529
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    After reform, CGIAR adjusted its strategy and organization, and the core research project (CRPs) has become the main organization form. How to cooperate with CRPs and CGIAR to improve the efficiency of cooperation and better serve the development of agricultural science and technology in China has become an urgent practical problem. On the basis of elaborating the reform of CGIAR organization mechanism and the operation mechanism of CRPs, this paper comprehensively analyzed the current situation of cooperation between Chinese Academy of Agricultural Sciences and National Natural Science Foundation Committee (NSFC) and CGIAR, and put forward the cooperation mechanism and key areas between China and CGIAR CRPs in the future.
    Construction of Insect and Herbicide Resistant Expression Vector Based on GR79 EPSPS Screening Marker and Identification of Resistance
    YANG Yang1,2, LIANG Chengzhen2, MENG Zhigang2, CHEN Quanjia1, LUO Shuping1, ZHANG Rui2, GUO Sandui2*
    2018, 20(11):  22-28.  DOI: 10.13304/j.nykjdb.2017.0819
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    Pests and weeds are 2 main hazards in agricultural production. At present, although transgenic insect and herbicide resistant crops have been reported and applied, cultivation of crops with resistance both to insect and herbicide in China is still lagging behind. The traditional hybrid breeding means are mainly adopted, which are energy/time consuming with long cycle. According to the binding and toxicity domain of Bacillus thuringiensis (Bt) Cry1Ac protein, this study synthesized the insecticidal gene cry1Ac-2.5; used GR79-EPSPS gene to replace the kanamycin screening marker; and build the insect and herbicide resistant plant expression vector based on glyphosate herbicide screening marker, then transformed to tobacco. The results of real-time fluorescence quantitative PCR (qRT-PCR) showed that transgenic tobacco with cry1Ac-2.5 and GR79-EPSPS gene were expressed successfully at transcription level. The further Cry1Ac and GR79-EPSPS strips test showed that the above genes were translated correctly at the protein level. The resistance experiment showed that the transgenic tobacco callus could be screened by glyphosate. The positive rate was 80%, and transgenic tobacco was tolerant to treatment with 100 mg/L herbicide. The insect-resistant experiments showed that the mortality of Helicoverpa armigera larvae was about 90% 4 d after feeding Cry1Ac-2.5 transgenic tobacco for 4 d, indicating that the artificial gene cry1Ac-2.5 was significantly insect-resistant. All above results showed that the insecticidal herbicide plant expression vector (Cry1Ac-2.5+GR79 EPSPS) constructed in this study had combined the plant screening markers and herbicide resistance effectively. This study was of guiding significance for rapid cultivation of insect and herbicide resistant crops.
    Optimization of Fermentation Condition and Application Method of VIGS Biocontrol Agent Against Potato Virus Y
    LIU Tianbo1,3, CAI Hailin2, ZENG Weiai2, WEI Runjie1, LIU Jiayu1, LI Fan2, ZHOU Zhicheng3, TANG Qianjun1*
    2018, 20(11):  29-35.  DOI: 10.13304/j.nykjdb.2017.0871
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    Virus-induced gene silencing (VIGS) is a widely applied natural mechanism for plant defense against virus, which can effectively prevent potato virus Y disease. Taking the control effect of VIGS expression vector fermentation broth on potato virus Y disease as detection index, this study screened the culture medium needed by fermenting the best fermentation broth, fermentation conditions and application methods. The results showed that the optimal medium was YEP (yeast extract broth peptone), optimal fermentation conditions were medium volume 60 mL/200 mL, initial pH 7.0, fermentation temperature 30℃ and shaking culture time 24 h. The optimal application methods were dilution 100 times, “leaf spraying + transplanting root irrigation”, and “mixture of VIGS biologics containing PVY CP and HC-Pro gene fragments”. The above results provided a theoretical basis for fermenting VIGS biocontrol agent, and layed a foundation for the field application.
    Correlation Analysis Between Expression Levels of Biosynthetic Genes for Secondary Metabolites and Their Contents in Rehmannia glutinosa
    ZHU Yunhao1,2, LI Lu1, ZHAO Le1,2, DONG Chengming1,2*
    2018, 20(11):  36-43.  DOI: 10.13304/j.nykjdb.2017.0664
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    In order to study the relative expression of the biosynthetic genes for secondary metabolites in different materials of Rehmannia glutinosa, and analyze the relationship between gene expression and secondary metabolites accumulation, this paper extracted total RNA from leaves, leaves of tissue culture seeding and hairy roots of R. glutinosa, respectively. TIP41 was used as a reference gene, and the relative expression of the biosynthesis-related enzymes genes for catalpol and verbascoside were analyzed by real-time quantitative PCR. The content of catalpol and verbascoside in 3 kinds of material were measured by HPLC, respectively, while the content of total iridoid glycoside was measured by UV spectrophotometry, and then the correlation between their contents and relative expressions of the genes related to biosynthesis enzymes were analyzed. The results indicated that there were more differences in the expression levels of the genes related to biosynthesis enzyme for secondary metabolites and their contents among the 3 different kinds of R. glutinosa materials. According to relative correlation analysis, the content of catalpol was significantly and positively correlated with the expression quantity of GGPPS2, and the content of total iridoid glycoside was also significantly correlated with the expression quantities of GGPPS1 and G10H, indicating that GGPPS2 was related with the synthesis of catalpa, GGPPS1 and G10H might be related to total iridoid glycoside biosynthesis. While, there were no significant correlation between the content of verbascoside with the expression levels of ADT, ASP5, 4CL, C3H, HCT and GOT2. These results provided references for relative studies on secondary metabolites biosynthetic pathways of R. glutinosa.
    Effects of Nitrogen Application on Glutamine Synthetase Isozymes and Nitrogen Metabolism of Flue-cured Tobacco in Nanyang Tobacco-growing Areas
    ZHOU Jianfei, WU Yunjie, XUE Gang, XU Shixiao, YANG Tiezhao*
    2018, 20(11):  44-53.  DOI: 10.13304/j.nykjdb.2017.0762
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    In order to investigate the appropriate nitrogen application amount for fragrant-flavor tobacco in Nanyang tobacco-growing areas, this paper studied the expression characteristics of glutamine synthase (GS) isozyme protein and the changes of related physiological indexes at mature stages of different cultivars using split zone test design and treated by 3 nitrogen application treatments. The results showed that the activities of GS1, GS2 and NR at maturity stage of NC71 were lower, GDH activity was higher, nitrogen assimilation and reutilization ability were also lower. At the same time, the content of NH+4 in apoplast and ammonia volatilization were both high, thus having high nitrogen efficiency in mature stage, and 52.5~60.0 kg/hm2 pure nitrogen application was reasonable. Yunyan 99 had higher GS1 activity at mature stage, nitrogen degraded by aging was reused in a large quantities, and the ammonia volatilization was also low, therefore, the total nitrogen content in leaves was higher, aging could be easily delayed. It was ideal to apply 25~30 kg/hm2 of pure nitrogen. The nitrogen efficiency of “Qushou 1” was between the other 2 varieties. It was suit to apply 40~45 kg/hm2 of pure nitrogen. Correlation analysis showed that there was a significant or extremely significant correlation between GS isoenzyme activity and leaf nitrogen metabolic index, which could be used as an important basis for studying nitrogen metabolism characteristics of flue-cured tobacco.
    Detection and Analysis of Florfenicol-resistant Genes and Their Genetic Components in Staphylococcus From Different Parts of Pig
    YAO Xiaohui§, CAI Jianxing§, SU Zhanqiang, GAO Chao, XUAN Huiyong, XIA Lining*
    2018, 20(11):  54-61.  DOI: 10.13304/j.nykjdb.2017.0883
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    Florfenicol is a special antibacterial agent for animals. However, with the increase of clinical dosage, the number of florfenicol-resistant strains has also been increasing year by year. Therefore, its transmission mechanism needs to be studied. This study collected samples of pig rectum and nasal cavity in the surrounding farms in Urumqi of Xinjiang, and used agar dilution method to test the susceptibility of 9 antimicrobial drugs to Staphylococcus isolated from the samples. The florfenicol-resistant test was performed by PCR to detect the resistance genes cfr, fexA and fexB, and identifie the species of Staphylococcust. The moving component IS21-558 and Tn558 transpose genes causing their transmission were detected and analyzed to explore the possible causes of the above-mentioned drug resistance genes among the bacteria. The results showed that Staphylococcus from nasal cavity and rectum had higher resistance rates to clindamycin and ofloxacin, while the resistance rate to gentamicin was lower; Staphylococcus from nasal cavity was mainly resistant to 6 (19.9%) and 7 (19.9%); while Staphylococcus from rectum was mainly resistant to 8 (27.1%); Staphylococcus from nasal cavity was more resistant than Staphylococcus from rectum. Two strains carrying cfr and fexA genes were Staphylococcus sciuri and Staphylococcus xylose, respectively. One strain carrying fexA gene was Staphylococcus saprophyticus. The fexB gene was not detected. All of the positive strains were carrying part of IS21-558 moving component and transpose Tn558, which may increase the risk of horizontal transmission of cfr and fexA genes, making it possible to produce multidrug-resistant bacteria. This study reveals that the monitoring and detection of cfr and fexA genes should be strengthened in animal husbandry and veterinary clinic, and the basic data should be provided for the further analysis of the spread and epidemic of positive resistant strains.
    Life Lifting Scheme for Plant Protection UAV Based on Solar
    XU Weicheng1, CHEN Kai1, ZHANG Ming1, LI Zhengfeng2, ZHANG Tianshun1*
    2018, 20(11):  62-68.  DOI: 10.13304/j.nykjdb.2017.0768
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    For the needs of UAV in agriculture field, limited battery life is undoubtedly one of the important issues facing the plant protection unmanned aerial vehicle (UAV). Due to the bottleneck in battery producing technology, the effective operating time of plant protection UAV is mostly limited to about 12 min which is difficult to break through. As an inexhaustible “non source” power, the sun has gained special attention. This paper designed a scheme for flight duration of plant protection UAV based on solar energy. Feasibility of this scheme was tested on the six rotor UAV platform. The results showed that the flight life time of UAV installed on solar module was averagely increased 70 s compared with that before the installation. This design based on multi-axis UAV flight platform, which converted the solar energy into electricity to charge lithium batteries during UAV flight, thereby reducing the lithium battery consumption, and prolonging the effective operating time of the plant protection UAV. To a certain extent, it alleviated the problem existing in current unmanned aerial vehicle (UAV), and could be extend to application of other platforms based on the UAV.
    Design of Biomass Carbonization Equipment for Vertical Moving Bed
    HUANG Yu, FAN Qizhou*, WANG Lie, FAN Xianhong
    2018, 20(11):  69-78.  DOI: 10.13304/j.nykjdb.2017.0792
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    China is rich in straw resources, but the phenomenon of burning straw is very severe. It not only wastes resources, but also brings about a series of environmental problem. So this paper designed a kind of upright moving bed of biomass carbonization equipment taking crush biomass granules as material for biomass carbonization, by a innovative route with step-by-step heating and carbonization process. This equipment adopted smoldering and indirect heating method to provide heat for biomass carbonization, and used the screw conveyor, rotary grate and other structures to achieve the continuous carbonization of biomass. This paper introduced in detail the design process of the equipment from the whole to the part, and analyzed its important parameters. At the same time, the paper carried out strength analysis on important structures. In the process of manufacturing and assemblying the equipment, it was found that the trial equipment was designed well, easy to assemble, operate smoothly and the modular design part was easy to install and for later stage maintenance. The paper also conducted a functional test taking rice husk as carbonization raw material. The result showed the grain husk carbonization had good effect, indicating its process route had reasonable design. The equipment had reasonable structure, easy to operate, could sustain continuous production, and had certain market prospect.
    Compensation Benefits Analysis for Oxygen Supply of Bast Fiber Seedling Film Matted on the Bottom Surface of Seedling Plate
    ZHOU Wanlai1,2,3, YI Yongjian1, TAN Zhijian1, WANG Hongying1, YANG Yuanru1, YU Wang1, NIU Yanbin1, YI Zhenxie2,3, WANG Chaoyun1*
    2018, 20(11):  79-84.  DOI: 10.13304/j.nykjdb.2018.0290
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    Bast fiber seedling film is a new film material used for raising rice seedling by machine transplanting. Great deals of application practice have indicated that matting bast fiber seedling film on the bottom surface of seedling plate of seedling plug machine could stimulate the growth and development of seedling root system and improve seedling quality. However, its action mechanism is still unclear. This paper measured and analyzed the micro morphological characteristics of bast fiber seedling film, and investigated the compensation effect of oxygen supply to bottom plate through directly testing oxygen concentration at the bottom surface of seedling plate, combined with simulated demonstration method. The results showed that bast fiber seedling film could quickly compensate for oxygen consumption at the bottom of seedling plate. After the external oxygen entered the hole, microporous diffusion occured in the seedling film. Then oxygen was transfered to entire film surface, where oxygen could directly contact rice roots, ultimately increase the number of root system and oxygen uptaken, and then an effect similar to aerobic cultivation was achieved.
    Finite Element Analysis of Stalk Cutting of Chinese Little Greens Based on ANSYS and Verification Test
    LIU Dong, XIAO Hongru*, JIN Yue, YANG Guang
    2018, 20(11):  85-93.  DOI: 10.13304/j.nykjdb.2018.0224
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    Cutting device is a key part in orderly harvesting Chinese little greens. In order to reduce the loss and damage rate during this process and improve harvest efficiency, this paper measured and analyzed a series of mechanical properties of Chinese little greens stalks under compression condition by static measurement method. The results showed that the range of stalk axial elastic modulus was 3.15~3.91 MPa, and radial elastic modulus range was 20.24~20.99 MPa. The relevant values of the mechanical properties of Chinese little greens stalks were combined with the finite element analysis software ANSYS to establish a stalk material library, and then the 3-dimensional modeling software SolidWorks was used to construct the model for cutting process, which was imported into ANSYS for displaying dynamics analysis and verifying the results obtained from finite element analysis in field experiment. The comparison results showed that when knife reciprocating speed was more than 0.40 m/s, the stalks of Chinese little greens could be effectively cut with tidy cutting stubble and smooth fractures. These analysis results provided theoretical basis for future designing and parameter optimization of the components of cutting Chinese little greens.
    Study on Saline-alkaline Tolerance Ability and Evaluation Index Selection for Corn
    JING Yupeng1,2, LIAN Haifei1, LI Huanchun1, SHI Pei1, DU Chao3, LIU Mei4, CHANG Xinjuan5
    2018, 20(11):  94-104.  DOI: 10.13304/j.nykjdb.2018.0036
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    In order to explore the ability of saline-alkaline tolerance and the evaluation indexes of 6 maize varieties in the Tumochuan plain, this paper used indoor germination and outdoor sand training test to study the effects of mixed saline-alkali stress on the physiological and biochemical characteristics of maize seed germination and seedling growth. The results showed that: Under mixed salt and alkali stress, the germination time was delayed and the germination process was prolonged. With the increase of saline-alkali stress intensity, the seed germination rate and embryo growth of maize showed a significant trend of decrease; the salt damage index gradually increased; the degree of inhibition on the growth of maize plants also gradually increased; the content of malondialdehyde (MDA), proline and superoxide dismutase (SOD) in young leaf increased gradually; soluble protein content decreased gradually. The salt resistance of 6 maize varieties were: Yulong 2 > Jidan 535 > Yulong 8 > Dongdan 969 > Nongfu 9 > Nanbei 1. According to the principal component analysis results of 11 evaluation indexes of saline-alkali resistance, seed germination rate, embryo growth, plant height, biomass and proline content were finally selected as the characteristic factors to evaluate the saline-alkali resistance of maize.
    Research on High Yield Cultivation of Morus alba and Its Pulping and Papermaking
    JI Liuyang1, FAN Yongming2, ZHAO Tianbang1*
    2018, 20(11):  105-111.  DOI: 10.13304/j.nykjdb.2018.0522
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    In order to explore the high yield cultivation method of Morus alba, the effects of fertilizer application, planting density, the age of foundation pile and the number of remaining strips on the yield of Morus alba were studied with Husang 32 as the material. The results showed that under the condition of organic fertilizer application 30 000 kg/hm2 and planting density 30 000 plants/hm2, the yield of Morus alba was relatively high. Meanwhile, in order to analyze the utilization value of Morus alba in the papermaking industry, the physical strength of pulping and papermaking was tested with 5 species of Morus alba, Populus tomentosa, Populus canadensis cv. Sacrau 79, Populus daknensis, and Paulownia. It was found that most of the physical strength indexes of Morus alba were excellent, and the physical and chemical properties of Morus alba slurry met the national standards through the improvement of chemical machine slurry making andthe Morus alba bleaching pulp had excellent performance. Above results laid a foundation for the high-yield cultivation of Morus alba and the development and utilization of paperrmaking resources.
    Effects of Exogenous Hydrogen Sulfide on Physiological and Biochemical Characteristics of Tobacco Seedlings under Drought Stress
    ZAHNG Jie, MA Xiaohan, CHEN Biao, XU Zicheng*, WANG Yinghui
    2018, 20(11):  112-119.  DOI: 10.13304/j.nykjdb.2017.0818
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    In order to clarify the alleviating effect of exogenous hydrogen sulfide (H2S) on tobacco seedlings under drought stress, using tobacco variety Yuyan 10 as research object, and sodium hydrogen sulfide (NaHS) as donor of exogenous hydrogen sulfide, this paper studied the effects of different NaHS concentration (0.01 mmol/L, 0.1 mmol/L, 0.4 mmol/L, 0.7 mmol/L, 1 mmol/L) on the physiological and biochemical characteristics of tobacco seedlings under 15% PEG-6000 simulated drought stress. The results showed that: compared with the control, the growth of tobacco seedling under drought stress was significantly affected. Compared with drought stress, the moisture content, chlorophyⅡcontent of tobacco seedling and root vigor, etc. indexes were significantly increased after the pretreatment of adding exogenous hydrogen sulfide, especially the proline content increased significantly. The water absorption and retention of seedlings were strengthened. At the same time, the activities of many antioxidants in body(SOD, POD, CAT, APX)were significantly strengthened. The leaf electrical conductivity, the contents of hydrogen peroxide ( H2O2) and malondialdehyde (MDA) in tobacco seedlings were all decreased, thus reducing the damage to tobacco seedlings caused by rising content of reactive oxygen species (ROS) under drought stress. After pretreating tobacco seedlings with exogenous hydrogen sulfide, the response of various physiological indexes had obvious gradient effect. Results showed that 0.4 mmol/L NaHS treatment had the best effect. Exogenous hydrogen sulfide enhanced the adaptability of tobacco seedlings to drought stress at certain extent, thus providing some basis for improving the drought resistance of tobacco seedlings.
    Influences of Different Stem-drying Temperature and Time on Quality and Sensory Evaluation of Toasted Sweet Aroma of Flue-cured Tobacco Leaf in Southern Anhui
    LIU Yaoxu1,2, CAI Xianjie3, CHENG Sen3, ZHU Qifa4, MA Chengxin4, HUANG Weiwei4, XU Xiuhong1, ZHANG Yuqin1,2, LI Qingshan1,2, WANG Chuanyi1*, LU Xiaohua3*
    2018, 20(11):  120-126.  DOI: 10.13304/j.nykjdb.2017.0612
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    In order to explore the relationship between different stem-drying temperature and time with quality and sensory evaluation of toasted sweet aroma of flue-cured tobacco leaf in southern Anhui,this paper studied the effects of different stem-drying temperature and time on aroma components and smoking quality of flue-cured tobacco, taking the middle leaf of Yunyan 97 —— a main cultivated variety as test material in Xuancheng-tobacco growing area of Anhui province. The results showed that with the increasing of stem-drying temperature, the appearance of cured tobacco and smoking quality tended to improve first and then became worse, and the main aroma components and toasted sweet aroma components showed the same trend. With the prolonging of stem-drying time, the appearance and smoking quality of cured tobacco showed a worsening trend, the contents of main aroma components and toasted sweet aroma components showed a reducing trend. The suitable stem-drying temperature and time (dry bulb temperature about 68℃, wet bulb temperature 41℃ and curing time about 24 h) were conducive to the display of flue-cured tobacco caramel flavor style.
    Environmental Impact Analysis of Biogas Slurry Generated by Pig Manure Anaerobic Treatment Based on CSTR
    LIU Jieyou1,2, QU Liang1,3*
    2018, 20(11):  127-134.  DOI: 10.13304/j.nykjdb.2017.0837
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    In order to explore the main components of biogas slurry and environmental impact of biogas slurry returning based on continuous stirred tank reactor (CSTR), this paper collected pig manure from 3 large-scale farms, biogas slurry produced by pig manure anaerobic fermentation treatment, and soil samples irrigated with biogas slurry, tested and analyzed the COD (chemical oxygen demand), BOD5, heavy-metal elements and nutritive elements in the samples. The results showed that the removal rates of COD and ammonia nitrogen in biogas slurry were 63%~82% and 37%~75%, respectively, after treating pig manure anaerobic fermentation based on CSTR. The contents of Zn and Cu in biogas slurry were relatively higher, which were 2.1×104 μg/L and 3.8×103 μg/L, respectively. The correlation coefficients for heavy-metal content between biogas slurry and fodder were 0.93~0.95. The increase of nutritive element in soil after applying biogas slurry was 0.3~14 times and heavy-metal content in soil could basically reached Ⅰ level soil standard. Rational application of biogas slurry could increase nutritive contents in soil, and there was no significant heavy metal pollution to soil. The heavy metal contents in edible part of crops planted in soil fertilized by biogas slurry could meet food safety requirement.
    Research on Adsorption Effect of Ammonia-nitrogen from Biogas Slurry by Modified Biochar
    MA Yanru1,2, MENG Haibo2, SHEN Yujun2, DING Jingtao2, WANG Liming1*
    2018, 20(11):  135-144.  DOI: 10.13304/j.nykjdb.2017.0764
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    Aiming at the problem of excessive nitrogen pollution in biogas slurry, and in order to screen out the effective adsorption method for maximum recovery of nitrogen, this study took corn stalk, scorn cob and sawdust as raw material, and produced biochar by pyrolysis at 550℃, 600℃, 650℃ and modified them with NaOH+microwave, FeCl3, KOH and HNO3, separately. The biochar was characterized by scanning electron microscopy and mercury injection. The adsorption effect of biochar on NH+4-N was investigated by adsorption kinetics, adsorption isotherm and influencing factor experiment. The results showed that the adsorption equilibrium time of corn stalk modified by NaOH+microwave of 550℃(A-550-NaM), corn stalk modified by KOH of 550℃(A-550-K), sawdust modified by NaOH+microwave of 600℃(C-600-NaM)and scorn cob modified by FeCl3 of 550℃(B-550-Fe) were between 60~150 min, and their equilibrium adsorption quantities were 8.58 mg/g, 8.30 mg/g, 7.95 mg/g and 8.01 mg/g, respectively. Langmuir model was more suitable for describing the adsorption behaviors of NH+4-N by B-550-Fe, A-550-NaM and A-550-K than Freundlich model. The maximum adsorption capacity of these 3 kinds of modified biochars on NH+4-N were 200.24 mg/g, 101.86 mg/g and 94.82 mg/g, respectively.
    Expression of Seven Genes Involved in Salinity Adaption in Apostichopus japonicus under Low Salinity Stress
    SHANG Yanpeng, TIAN Yi*, LI Xiaoyu, JIANG Yanan, CHANG Yaqing
    2018, 20(11):  145-153.  DOI: 10.13304/j.nykjdb.2017.0789
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    Seven genes involved in salinity adaption, including Tenascin-R1 (TN-R1), Tenascin-R2 (TN-R2), Neuronal acetylcholine receptor subunit alpha-3 (CHRNA3), Fatty acid-binding protein 6(FABP6), Monocarboxylate transporter 2 (SLC16A7), Ficolin-1(FCN1), and Melanotransferrin (Mfi2) were selected based on salty transcriptome database of Apostichopus japonicus. The expression profiles of these 7 genes in different tissues and different periods under low salt stress were analyzed by qRT-PCR. The results showed that the expression levels of TN-R1, SLC16A7, FABP6 and Fcn1 was the highest in the coelomic fluid, followed by respiratory tree and intestinal tissue. No expression of TN-R2 was detected in respiratory tree. The expression levels of CHRNA3 and Mfi2 were the highest in coelomic fluid, followed by intestinal tissue and respiratory tree. The expression levels of these 7 genes increased or decreased during low salt stress. Gene Mfi2 was up-regulated in the coelomic fluid and significantly down-regulated in the respiratory tree and intestinal tissue. The expression of FCN1 in the coelomic fluid reached the highest level after low salt stress for 1.5 h, 669 times higher than that of the control group. The changes of these 7 genes under low salt stress showed that these genes were either directly involved in the metabolic regulation as functional proteins or as a regulated protein to regulate the expression and activity of functional proteins to improve adaption to low salt stress. The results suggest that the salinity adaptation process of sea cucumber is a stress response signal transduction network requiring multiple genes. These results laid a foundation for studying the salinity adaptation mechanism of Apostichpus japonicus.