基因编辑猪的研究现状

doi:10.13304/j.nykjdb.2021.0671

中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (8): 25-34.DOI: 10.13304/j.nykjdb.2021.0671

基因编辑猪的研究现状

翟利敏¹(), 李文通², 冯政¹, 李华¹, 裴杨莉¹()

^1.佛山科学技术学院生命科学与工程学院，广东省动物分子设计与精准育种重点实验室，广东普通高校动物分子设计与精准育种重点实验室，广东佛山 528225
^2.中国农业科学院深圳农业基因组研究所，广东深圳 518120

收稿日期:2021-08-08 接受日期:2022-01-18 出版日期:2022-08-15 发布日期:2022-08-22
通讯作者: 裴杨莉
作者简介:翟利敏 E-mail： 1772726298@qq.com；
基金资助:
广东省动物分子设计与精准育种重点实验室项目(2019B030301010);广东普通高校动物分子设计与精准育种重点实验室项目(2019KSYS011);广东省基础与应用基础研究基金项目(2020B1515120016)

Current Status of Gene-edited Pigs

Limin ZHAI¹(), Wentong LI², Zheng FENG¹, Hua LI¹, Yangli PEI¹()

^1.Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding，Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes，School of Life Science and Engineering，Foshan University，Guangdong Foshan 528225，China
^2.Agricultural Genomics Institute at Shenzhen，Chinese Academy of Agricultural Sciences，Guangdong Shenzhen 518120，China

Received:2021-08-08 Accepted:2022-01-18 Online:2022-08-15 Published:2022-08-22
Contact: Yangli PEI

摘要/Abstract

摘要：

猪是重要的农业经济动物，猪肉是人类获得蛋白质营养物质的主要途径之一。与此同时，猪在解剖学、生理学及遗传背景、疾病特征等方面与人类极为相似。因此，猪既是农业动物生产性状改良的重要对象，又是人类疾病、异种器官移植等生物医学领域的研究对象。随着基因编辑技术的飞速发展，出现了越来越多操作简单、运用广泛且安全的新型编辑技术，可以快速获得单碱基编辑、基因敲除或敲入的细胞系，并通过体细胞克隆等技术获得基因编辑猪。综述了基因编辑猪的制备及其在农业及医学领域中的研究进展，以期为猪的农业生产性状改良和医学研究提供参考。

关键词: 基因编辑, 猪, 生产性能, 疾病模型, 器官供体

Abstract:

Pigs are important agricultural economic animals， and pork is one of the main protein source for human population. At the same time， pigs closely resemble humans in terms of anatomy， and genetic background， physiology， disease characteristics. Therefore， pigs are not only important objects for improvement of production traits， but also biomedical research objects in the field of human diseases and xenotransplantation. With the rapid advances in genome editing technology， there are new editing technologies that are widely available， simple to perform and safe. These new gene editing techniques can be used to quickly obtain cell lines with single-base editing， gene knock-in or knock-out， then gene-edited pigs can be obtained by somatic cloning and other techniques with those edited cell line. This paper reviewed the preparation of gene-edited pig， and the research progress of gene-edited pigs in the fields of agriculture and medicine， which was expected to provide reference for the agricultural production traits improvement and medical research of pigs.

Key words: gene-edited, pig, production performance, disease model, organ donor

中图分类号:

S828

翟利敏, 李文通, 冯政, 李华, 裴杨莉. 基因编辑猪的研究现状[J]. 中国农业科技导报, 2022, 24(8): 25-34.

Limin ZHAI, Wentong LI, Zheng FENG, Hua LI, Yangli PEI. Current Status of Gene-edited Pigs[J]. Journal of Agricultural Science and Technology, 2022, 24(8): 25-34.

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基因编辑猪的研究现状

Current Status of Gene-edited Pigs

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