中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (7): 54-71.DOI: 10.13304/j.nykjdb.2024.0101

• 生物技术 生命科学 • 上一篇    下一篇

大豆种质资源苗期耐碱筛选及评价分析

郑焕斌1,2(), 李明3, 杨素欣2(), 吴委林1()   

  1. 1.延边大学农学院,吉林 延吉 133002
    2.中国科学院东北地理与农业生态研究所,大豆分子设计育种 重点实验室,长春 130102
    3.吉林农业大学生命科学学院,长春 130118
  • 收稿日期:2024-02-04 接受日期:2024-03-20 出版日期:2025-07-15 发布日期:2025-07-11
  • 通讯作者: 杨素欣,吴委林
  • 作者简介:郑焕斌 E-mail:zhenghuanbin2024@163.com
  • 基金资助:
    国家重点研发计划项目(2022YFD1500503)

Alkaline Tolerance Screening and Evaluation Analysis of Soybean Germplasm Resources at Seedling Stage

Huanbin ZHENG1,2(), Ming LI3, Suxin YANG2(), Weilin WU1()   

  1. 1.College of Agriculture,Yanbian University,Jilin Yanji 133002,China
    2.Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China
    3.College of Life Sciences,Jilin Agricultural University,Changchun 130118,China
  • Received:2024-02-04 Accepted:2024-03-20 Online:2025-07-15 Published:2025-07-11
  • Contact: Suxin YANG,Weilin WU

摘要:

为探究不同大豆种质资源苗期对于碱胁迫处理的耐受性,建立大豆苗期耐碱性评价体系,以326份育成大豆品种作为试验材料,在pH 9.0、150 mmol·L-1 的混合碱(NaHCO3∶Na2CO3=5∶1)溶液处理7 d后,测定处理和对照植株地上部分干/鲜重、地下部分干/鲜重、植株总干/鲜重、完整根系数量和长度、根尖数量、根冠比、叶绿素相对含量和植株水分含量共12个生长指标。结果表明,除根冠比外,碱胁迫使各参试大豆品种苗期生长指标显著降低。利用主成分分析和模糊隶属函数法计算得到耐碱综合评价值(D值),根据D值将326份大豆品种划分为4个类群,分别为极端耐碱型(18份)、耐碱型(140份)、敏碱型(130份)和极端敏碱型(38份),同时建立了大豆苗期耐碱性综合评价体系。以上研究结果为耐碱性大豆品种的筛选和评价提供了理论依据。

关键词: 大豆, 耐碱性, 种质资源筛选, 综合评价

Abstract:

In order to explore the tolerance of different soybean germplasm resources to alkali stress treatment at seedling stage, and establish the evaluation system of soybean alkaline tolerance at seedling stage, 326 soybean varieties were as materials. They were treated with the pH 9.0, 150 mmol·L-1 mixed alkali solution (NaHCO3∶Na2CO3=5∶1) for 7 d. The changes of 12 growth-related indexes under different treatments, such as dry/fresh weight of shoot or root, total dry/fresh weight, intact roots number intact roots length, root tips number, root/shoot ratio, relative chlorophyll content, and water content of plants were determined. The results showed that alkali stress significantly reduced the growth indexes of soybean varieties at seedling stage except root/shoot ratio. The comprehensive evaluation value (D value) of alkali tolerance was obtained by principal component analysis and fuzzy membership function method. According to the D value, 326 soybean varieties were classified into 4 groups: extremely alkali tolerant varieties (18 varieties), alkali tolerant varieties (140 varieties), alkali sensitive varieties (130 varieties) and extremely alkali sensitive varieties (38 varieties), and the comprehensive evaluation system were established. Above results provided theoretical basis for the selection and evaluation of alkaline resistant soybean varieties.

Key words: soybeans, alkali tolerance, germplasm screening, comprehensive evaluation

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